Journal of Hunan Normal University(Medical Science)

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Effect of Chinese Acupuncture and Moxibustion Combined with Massage on Cervical Spondylosis of Vertebral Artery Type and Its Effect on Quality of Life of Patients

Fu Yao

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 149-152.

Abstract (16289) PDF (10925)

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Objective To study the effect of Chinese acupuncture and moxibustion combined with massage therapy on cervical spondylosis of vertebral artery and its effect on the quality of life of patients. Methods 200 cases of patients with vertebral artery type cervical spondylosis who were treated in our hospital from March 2017 to March 2017 were selected as the subjects. All the patients were divided into control group and study group according to the random number table method, each group of 100 cases. The patients in the control group were received traditional Chinese medicine acupuncture treatment, and the patients in the study group were given traditional Chinese medicine acupuncture combined with massage therapy. The clinical symptom scores, quality of life improvement and clinical efficacy were compared between the two groups before and after treatment. Results There was no significant difference in the clinical symptom scores between the two groups before treatment. After treatment, the shoulder and neck pain, dizziness and headache scores of the two groups were higher than those before treatment, and the study group was higher than the control group, the difference was statistically significant; There was no significant difference in QLQC30 score between the two groups before treatment. The QLQC30 scores of the two groups were higher than those before treatment, and the study group was higher than the control group, the difference was statistically significant; The total effective rate was 95.0% (95/100) in the study group and 86.0% (86/100) in the control group, the difference was statistically significant. Conclusion Chinese Acupuncture and moxibustion combined with Massage on Cervical Spondylosis of Vertebral Artery Type effect is significant, can effectively relieve clinical symptoms, improve the quality of life of patients, it is worth promoting.

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Screening and verification of miR-148a-3p target gene

Luo Yan-hong, Zhang Shan, Peng Xiao-ning

HuNan ShiFan DaXue XueBao(YiXueBan).

Abstract (4121) PDF (27508)

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目的：筛选及验证 miR-18a-3p 的靶基因，为深入研究 miR-18a 的功能建立基础。方法：我们通过miRanda、Target Scan 和 PicTar 等多种生物信息学方法对 mir-18a-3p 的靶基因进行了预测分析，取共同交集的靶基因；运用生物素标记的 RNA 进行 RNA-RNA pull down 来验证优选得到的靶基因；构建过表达 miR-18a-3p 及干扰 miR-18a-3p 表达的稳定神经胶质瘤细胞株，用荧光定量 PCR 实验比较神经胶质瘤与正常胶质细胞中靶基因的表达水平及比较过表达和干扰表达与神经胶质瘤细胞株中靶基因的表达水平。结果：多种生物信息学网站预测 gadd5a 为其共同交集的靶基因之一；qRT-PCR 结果表明神经胶质瘤中 gadd5a 的表达较正常胶质细胞低， miR-18a mimics 及 miR-18a inhibit 转染入神经胶质瘤细胞系后，过表达 miR-18a 的神经胶质瘤细胞中 gadd5a 较未转染的表达低；RNA pull down 结果则表明GADD5a 是miR-18a-3p 的靶基因。结论：GADD5A 是神经胶质瘤中mir-18a-3p 的靶基因之一，mir-18-3p 可能通过调控靶基因gadd5a 的表达，促进神经胶质瘤细胞的迁移与侵袭。

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To discuss the clinical significance of the changes of PA and RBP’s Levels in Sepisis of Children

ZOU Li-Li, LI 　Yun

HuNan ShiFan DaXue XueBao(YiXueBan). 2015, 12(2): 31.

Abstract (2078) PDF (11427)

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Objective To discuss whether prealbumin (PA)and retinol-binding protein(RBP) in early stage of liver and renal
injury were significative to sepsis in children. Methods 100 sepsis patients were enrolled from the respiratory ward of Children’
s Medical Center in Hunan Provincial People’s Hospital from February 2014 to October 2014,and among them above 90 cases
were full compliance with the standard.98 cases were full compliance with the standard in 100 cases with non-sepsis. Took 80
healthy children in the same period by physical examination as normal control group. Measured the concentrations of the liver
function, renal function, PA and RBP using Hitachi 7600 automatic biochemical analyzer .Collected relevant clinical data of the
three groups for statistical analysis at the same time. Results The levels of PA and RBP in Sepsis group were significantly lower
than non-sepsis group and the normal group.The levels of PA and RBP in the Non-sepsis group were slightly lower than the normal
group, but the difference was not statistically significant.In sepsis group ,the levels of WBC count, N%, CRP, BS, ALT were
higher than non-sepsis group and the normal group, and ALB in sepsis group were lower than other two groups，and the differences
of them were all statistically significant.There were no significant statistical differences, in other indicators including Cr,
BUN, TBIL , dBIL. Conclusion The changes of the levels of PA and RBP may participate in the early damage of liver and renal
fμnction.The decrease in the levels of PA and RBP could be used for finding the early damage of organ in Children’s sepsis.

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The Effect of 7-Difluoromethoxy-5，4'-Dimethoxygenistein on COX-2 Expression and cell adhesion in HUVE-12 Cells

ZHANG 　Jing, ZHANG 　Yong, LIU Shi-Wei, HUANG 　He, YANG Shu-Ting, FU Xiao-Hua

HuNan ShiFan DaXue XueBao(YiXueBan).

Abstract (2068) PDF (3623)

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目的:评估7-二氟甲氧基-5,4'-二甲氧基金雀异黄素(7-difluoromethoxy-5,4’-dimethoxygenistein,DFMG) 对损伤的人脐静脉内皮细胞( HUVE-12)中环氧化酶2 (cyclooxygenase-2, C0X-2)蛋白表达的影响及对HUVE-12细胞氧化损伤的保护作用。方法:肿瘤坏死因子-α（Tumor necrosis factor-α,TNF-α)诱导建立 HUVE-12损伤模型，不同浓度的DFMG对其进行诱导；流式细胞术(Flow Cytometry,FCM)检测细胞凋亡、Western blotting检测COX-2蛋白表达、蛋白定量分析法测定内皮细胞/单核细胞的黏附率、ELISA检测E-选择素、单核趋化蛋白-1 ( Monocyte chemoattractant protein-1, MCP-1)的释放。结果: DFMG 能拮抗损伤的内皮细胞活性的下降，并呈浓度依赖性，同时DFMG也能呈浓度依赖性地下调COX-2蛋白的表达、下调内皮细胞与单核细胞的黏附率。结论: DFMG可能通过减少COX-2蛋白表达水平而减少单核细胞/内皮细胞黏附作用，进一步减少炎症渗出，拮抗炎症反应，保护血管内皮细胞。

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Efficacy and safety of celecoxib combined with methotrexate in the treatment of rheumatoid arthritis

Tang Jia

HuNan ShiFan DaXue XueBao(YiXueBan).

Abstract (1976) PDF (11753)

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Objective To study the efficacy and safety of celecoxib combined with methotrexate in the treatment of rheumatoid arthritis. Methods A total of 100 patients with rheumatoid arthritis who were treated in our hospital from April 2014 to May 2016 were selected as subjects. All patients were divided into study group and control group according to the random number table method, each group of 50 cases. The control group was treated with methotrexate. The study group was treated with celecoxib on the basis of the control group. The levels of arthritis, inflammatory factors (IL-1, IL-6), the number of joint swelling and the incidence of adverse reactions were observed and compared between the two groups before and after treatment. Results The VAS scores of the two groups were lower than those before the treatment, and the difference was statistically significant. The levels of IL-1 and IL-6 in the two groups were lower than those before treatment, and the difference was statistically significant. The number of joint swelling after treatment in the two groups was less than that before treatment, and the study group was less than the control group, the difference was statistically significant; There was no significant difference in the incidence of adverse reactions between the two groups ［8.0% vs12.0%］. Conclusion Celecoxib combined with methotrexate in the treatment of rheumatoid arthritis with significant efficacy, high safety, can effectively reduce the inflammatory response, reduce joint pain in patients with joint swelling, reduce the number of joint swelling, improve patient quality of life, worthy of clinical promotion.

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Disscussiong the machanism of SWI and DWI in T1WI high signal on neonatal Punctate white matter lesions

HuNan ShiFan DaXue XueBao(YiXueBan). 2015, 12(2): 7-10.

Abstract (1959) PDF (3502)

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Objective To probe the machanism of SWI and DWI in T1WI high singanl of neonatal Punctate white matter lesions. Methods From June 2012 to June 2013, 42 cases with PWML dignosed by MRI in our hospital was checkted by MRI (T1WI, T2WI and FLAIR), DWI and SWI sequence can scan. We disscuss the machanism of the high signal intensity in the T1WI. Results SWI in 38 cases of displayed medium signal, and there was no difference compared with the signals around. SWI in 4 cases underwent displayed low signal, and 1 case was premature infant and 3 cases was full-term children. There was no difference between preterm infants and full term by the chi-square test. 50.0% (21 cases) showed high signal on DWI, and between preterm infants and full term group, PWML in DWI on the incidence of brain hypoxic ischemia showed no statistical difference. Conclusion The high singal of T1WI in PWML was not meaned micro bleeding in most neonatal. T1WI signal was mainly associated with brain white matter hypoxic ischemia and hypoxic、ischemic -reperfusion and bleeding from the three kinds of pathological changes at the same time or at different stages.

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The expression of LRG1 gene in hepatocellular carcinoma

XI Zhen-Chang, ZHANG Jian-Song

HuNan ShiFan DaXue XueBao(YiXueBan). 2015, 12(2): 24-27.

Abstract (1959) PDF (2783)

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Objective To investigate the expression of leucine-rich-alpha-2-glycoprotein 1(LRG1 ) in hepatocellular carcinoma
(HCC) Cell lines and tissue, and to explore its relationship with HCC progression. Methods qRT-PCR and Western
blot analysis were used to detect LRG1 mRNA and protein expression respectively in 6 hepatocellular carcinoma cell lines, 30
paired HCC and adjacent normal liver tissues. Results The levels of LRG1mRNA and protein expression hepatocellular carcinoma
cell lines mRNA, protein levels LRG1 significantly higher than normal liver cells L02, the of invasion and metastasis
of HCC cell lines positive correlation with LRG1 expression levels. The levels of LRG1 mRNA and protein expression in HCC
were significantly higher than those in adjacent liver tissues. Conclusion LRG1 overexpression has a significant correlation
with the progression of HCC, and it would designing new targeted drugs.

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Study of circulating microRNA-4516 for diagnosis and prognostic valuation in hepatocellular carcinoma

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 5-10.

Abstract (1829) PDF (11221)

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目的：探讨循环 microRNA-51 在肝癌血浆及组织中的表达情况，研究其在肝癌诊断及预后中的作用。方法：采用实时荧光定量 PCR（qRT-PCR）方法检测 0 例肝癌患者和 2 例健康人（作为对照）血浆中 microRNA-51 以及 3 例肝癌组织和 23 例癌旁组织（作为对照）中 microRNA-51 的表达水平。结果：肝癌血浆及肝癌组织与对照组相比，肝癌患者血浆及癌组织标本中 microRNA-51 的表达量明显高于对照组，且差异有统计学意义。统计分析发现，在不同年龄、分期、肿瘤大小、AFP 水平以及 HBVDNA 等，患者间相对表达量差异均无统计学意义。miRNA-51 的表达与AFP 水平无明显相关性，但在AFP < 00μg/L 的肝癌患者中miRNA-51 的表达量明显高于正常人血浆及癌旁组织，且差异有统计学意义。Kaplan-Meier 分析提示 microRNA-51 表达水平高的患者具有较短的生存期限。结论：microRNA-51 可以用于肝癌的诊断，有可能作为一种新的肿瘤标志物，同时可以作为诊断肝癌及判断预后的参考。

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Effect of expression of SATB2 gene on proliferation and metastasis of colorectal cancer cells

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 24-28.

Abstract (1793) PDF (10826)

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目的：分析特定富含AT碱基DNA序列结合蛋白2（SATB2）对结直肠癌细胞增殖和转移能力的影响。方法：收集2014年9月~2016年9月期间在我院住院治疗的120例结直肠腺癌患者癌组织及其对应癌旁组织标本。均行免疫组织化学法检测，细胞培养与转染，采用实时荧光定量检测SATB2 miRNA的表达情况；并采用CCK-8法、细胞划痕实验分析SATB2干扰对SW480、LOVO细胞增殖能力与迁移能力的影响。结果：实时荧光定量PCR检测结果发现结直肠癌组织SATB2 miRNA的表达明显低于对应的癌旁组织；与免疫组织化学检测具有一致性，72.50%的结直肠癌患者癌组织SATB2呈低表达，而76.66%的结直肠癌患者癌旁组织SATB2呈高表达，两者差别有统计学意义。培养24h时，癌组织与癌旁组织中SW480、LOVO细胞的增殖速度比较无明显差异，而48h至72 h结直肠癌患者癌组织中SW480、LOVO细胞的增殖速度显著高于癌旁组织，差异有统计学意义。划痕实验结果显示癌组织SW480、LOVO细胞的细胞迁移能力较癌旁组织明显增强。结论：SATB2基因在结直肠癌癌组织和细胞中呈低表达，与结直肠癌SW480、LOVO细胞增殖和转移能力密切相关，故SATB2基因有可能成为结直肠癌新的治疗靶点。

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The research of indication value of PET imaging technique on A549-LUC2-GFP nude mouse#br# model tumor

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 15-18.

Abstract (1771) PDF (10757)

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目的：观察几种不同的分子影像学方法显示的A549 -LUC2-GFP 移植瘤裸鼠模型瘤体图像，探讨PET
成像在研究肿瘤生长特性及放疗靶区勾画方面的使用价值。方法：通过慢病毒介导构建能稳定表达荧光素酶和荧
光蛋白的人肺癌A549 -LUC2-GFP 细胞株，构建A549 -LUC2-GFP 裸鼠模型，分别在肿瘤生长的早期和后期进行荧
光成像、生物发光成像和micro 18F-FDG PET 亲肿瘤显像、micro CT 断层扫描成像，采集各种图像用计算机软件进
行处理和计算。结果：A549 -LUC2-GFP 细胞株荧光信号较强，发光稳定，生物发光成像的特异性和灵敏性高于荧
光成像。肿瘤种植后的第15 天，PET 所示肿瘤面积和周长的像素点多于生物发光，肿瘤生长第55 天PET 所示肿
瘤面积和周长的像素点少于生物发光。结论：裸鼠A549 -LUC2-GFP 模型能有效动态的监测A549 细胞的生长过
程，与荧光和生物发光图像相比，PET 图像在肿瘤早期诊断以及提示肿瘤细胞的生长状态和进展情况方面有其独
特的优势，提示核医学成像方法在更全面的描述肿瘤生长特性及确定放疗靶区方面有一定价值。
【关键词】 A549 -LUC2-GFP；生物发光成像；PET；肿瘤生物学特性；放疗靶区

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Clinical characteristics of 119 patients with Sheehan's syndrome：a retrospective study

CAO Wei-Juan, LIU Shi-Ping

HuNan ShiFan DaXue XueBao(YiXueBan). 2015, 12(2): 28-31.

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Objective To determine the clinical characteristics of 119 patients with Sheehan`s Syndrome (SS). Methods
We collected the clinical data of 119 inpatients diagnosed of SS in Department of Endocrinology, the Second Xiangya Hospital
, Central South University from January 2002 to November 2014. Results The mean period of diagnostic delay was（ 15.1±
11）years in these 119 patients with SS. 12 (10%) patients were pregnant again after being diagnosed with SS, 6（5%）of which
have long-term hormone replacement therapy, 1（0.8%） of which were pregnant again Get better. 64 (54%) developed pituitary
crisis and 55 (46%) did not, 48（75%）were pituitary crisis as first symptoms. 34（28.5%）had panhypopituitarism, while 85
（71.5%）had partial hypopituitarism. 101（97%）of the patients had gonadotropin deficiency, while 103（89%）of the patients had
TSH deficiency, and 51（100%）of the patients had ACTH deficiency. diabetes insipidus were 3 case（3%）. In 49 subjects having
magnetic resonance imaging of pituitary, 27 (55%) showed empty sella and 19 (39%) showed pituitary involution. Moreover, 40
subjects had bone mineral density examination, 10 (25%) of which showed osteoporosis and 23 (58%) of which showed osteopenia.
Conclusion Sheehan’s syndrome of severe situation is different, clinical manifestations are diverse, early correct diagnosis
is crucial. on the one hand, Prompt diagnosis and timely target gland hormone treatment can avoid pituitary crisis, osteoporosis,
etc, on the other hand, Sheehan’s syndrome of slight cases may be pregnant again, pregnant again after the SS can be improved
or alleviated.

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Polymorphism in Fas Promoter Region Associated with Risk to Breast Cancer

HuNan ShiFan DaXue XueBao(YiXueBan). 2015, 12(2): 4-7.

Abstract (1767) PDF (3310)

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Objective To evaluate the association between polymorphisms in Fas promoter region and susceptibility to breast cancer. Methods In the case-control study, we genotyped Fas-670A/G and Fas-1377A/G by PCR-RFLP in 214 patients with breast cancer and 204 healthy controls. Results were analyzed using Chi-square test. Fas gene expression wwas detected by q-RT-PCR in breast cancer. Results The distribution of Fas-670A/G genotype and allele type was significant difference between the patients and healthy controls. Subjects with GG genotype significantly increased the risk of breast cancer compared with the individuals carrying AA and AG genotype. Fas expression level in subjects with breast cancer was significantly increased compared with the healthy control. And the Fas expression in subjects with GG genotype was significantly higher than that in subjects with AA, GG genotypes. Conclusion Fas expression significantly increased in breast cancer. Fas was regulated by Fas-670A/G and significantly associated with susceptibility to breast cancer. GG genotype enhanced Fas expression and increased the risk to breast cancer.

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The study on the Clinical characteristic of diabetic foot and the analysis of clinic risk factors in#br# the severe diabetic foot

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 32-35.

Abstract (1757) PDF (10982)

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目的：回顾性分析我院糖尿病足的临床特点及医疗费用；并分析重型糖尿病足的相关危险因素，为糖尿病足的防治提供临床依据。方法：从2015年11月～2017年3月在长沙市第四人民医院内分泌科等相关科室就诊的2680例糖尿病患者中，选取符合纳入标准的170例糖尿病足（DF）患者病史采集、体格检查、生化检查及医疗费用等回顾性分析，并采用Logistic回归分析来探讨重型糖尿病足的危险因素。结果：糖尿病足的发病率为6.3%；170例糖尿病足患者中，其中1型糖尿病患者1例，2型糖尿病患者169例；男性92例，女性78例；92.8%的患者发病年龄>50岁；糖尿病病程平均101.27±27.89月；糖尿病足病程平均67.31±67.08天；空腹血糖平均水平为7.85mmol/L，平均HbA1c 水平为7.66%；103人受教育程度在高中以下；住院天数平均为25.07天；住院费用平均为19054.8元；其中66.9%患者患有糖尿病周围神经病变；57.6%患有糖尿病周围血管病变；34.1%患有糖尿病肾病；25.3%患有糖尿病视网膜病变。Wagner分级3-5级（重型糖尿病足）患者年龄、病程、空腹血糖，糖化血红蛋白，白细胞计数，SBP均高于0~2级（轻型糖尿病足）患者，而其ABI值、受教育程度均低于轻型。以上述临床资料为自变量，进行二分类Logistic回归分析示白细胞计数是发生重型糖尿病足的危险因素；高密度脂蛋白是发生重型糖尿病足的保护因素。结论：1、糖尿病足患者多发生在血糖、血压控制不满意，代谢紊乱，病程长，高龄，文化程度低的糖尿病患者。2、糖尿病足的发病率高。3、ABI值低、受教育程度低是重型糖尿病足的危险因素，白细胞数明显升高是重型糖尿病足患者的独立危险因素，高密度脂蛋白是其保护因素。

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Application of the color doppler ultrasound cardiogram examination in the diagnosis of acute
myocardial infarction

ZHU Wen-Bo-1, GAO 　Hui-2, SHI Jian-Bing

HuNan ShiFan DaXue XueBao(YiXueBan). 2015, 12(2): 43.

Abstract (1754) PDF (2508)

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Objective To investigate the color doppler ultrasound cardiogram examination in the diagnosis of acute myocardial
infarction (mi) value. Methods Select our hospital between February 2013 and February 2014, 76 cases of patients with acute myocardial
infarction (mi) as the research object, uneffective color doppler ultrasound cardiogram test, and the test results compared with
echocardiography and coronary angiography. Results The results of color doppler ultrasound cardiogram detection rate was 94.74%,
acute myocardial infarction compared with cardiogram difference was statistically significant. Conclusion Compared with echocardiography
color doppler ultrasound cardiogram in the diagnosis of acute myocardial infarction on more sensitive and accurate.

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Analysis of the effect of NGF, transforming growth factor β1, fibroblast growth factor in fracture healing

Wang Yu-xiang, Wang Wei-dong, Yang Ying-zhong,et al

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 95-98.

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Objective To investigate the effect of nerve growth factor (NGF), transforming growth factor β1(TGF- β1), fibroblast growth factor (FGF) in fracture healing. Methods Selected 30 male New Zealand rabbits, were randomly divided into 5 groups, including saline group, NGF group, TGF-β1 group, FGF group, NGF+FGF+TGF-β1 group, 6 rabbits in each group, tibial fracture model was established in all animals. The saline group, NGF group, TGF-β1 group, FGF group, NGF+FGF+TGF-β1 group after operation in the fracture site were injected saline, NGF, TGF-β1, FGF and NGF+FGF+TGF- β1, observed each group the X-ray score, bone mineral density and osteocalcin levels. Results Postoperative 14d, 21d and 28d, NGF group, TGF-β1 group, FGF group, NGF+FGF+ TGF-β1 group X-ray score, bone density and serum osteocalcin were significantly higher than those in the saline group; Postoperative 21d and 28d, NGF+FGF+TGF-β1 group X-ray scores were (15.63±0.92) and (16.01±0.93), significantly higher than that of NG group F, TGF-β1 group and FGF group; Postoperative 14d, 21d and 28d NGF+FGF+TGF- β1 groups bone density were (0.604±0.019) g/mm², (0.613±0.021) g/mm² and (0.644±0.016) g/mm², significantly higher than that of NGF group, TGF- β1 group and FGF group; Postoperative 14d, 21d and 28d NGF+ FGF+ TGF-β1 groups serum osteocalcin were (10.236±0.121) ng/ml, (11.924±0.093) ng/ml and (13.210 ±0.083) ng/ml, significantly higher than that of NGF group, TGF-β1 group and FGF group. Conclusion NGF, TGF-β1 and FGF has a certain role in promoting fracture healing, them has a strong synergistic effect, can obviously promote fracture healing.

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Effects of Feilike Mixture Assisted Budesonide Formoterol Powder Inhalants in the Treatment of Cough Variant Asthma and Airway Inflammation

Wang Chun-chang, Jin Yang, Yang Cui, et al

HuNan ShiFan DaXue XueBao(YiXueBan).

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Objective To explore the clinical efficacy of Feilike mixture combined with budesonide formoterol powder inhalants in the treatment of cough variant asthma (CVA) and its effect on airway inflammation. Methods A total of 72 patients with CVA enrolled in Respiratory Medicine Beijing First Hospital of Integrated Chinese and Western Medicine were selected for the study and randomly divided into the control group (n=36, treated with budesonide formoterol powder inhalants) and the experimental group (n=36, treated with budesonide formoterol powder inhalants and Feilike mixture). The clinical efficacy, pulmonary function, airway inflammation and adverse reactions were compared between the two groups. Results The total effective rate was 94.44% in the experimental group, which was significantly higher than that in the control group with 77.78%. After treatment, the day or nocturnal cough symptom scores, one-second forced expiratory volume (FEV1), FEV1/forced vital capacity (FVC), the ratio of PEF (PEF pred%) and interleukin-10(IL-10) level were significantly higher than those before treatment, After treatment, the levels of the eosinophil (EOS) percentage, inflammatory mediators interleukin-4(IL-4) and interleukin-5(IL-5) were significantly decreased in the two groups, and the changing degree in the experimental group was significantly higher than that in the control group. There was no significant adverse reaction between the two groups as well as the incidence rate of adverse reactions. Conclusion The use of Feilike mixture combined with budesonide formoterol powder inhalants can have significant effects in the treatment of CVA children, such as relieving the cough symptoms of children and improving lung function and airway inflammation with high safety.

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miR-124 expression in the brain and serum of chronic unpredictable mild stress mice

HuNan ShiFan DaXue XueBao(YiXueBan).

Abstract (1726) PDF (10784)

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目的：本研究旨在探究慢性应激对小鼠脑内和血清 miR-12 表达的影响。方法：通过每天随机给予小鼠束缚、潮湿垫料等应激原，持续 8 周，建立慢性不可预知温和应激模型。通过强迫游泳、蔗糖偏好动物行为学试验评估应激组和对照组小鼠抑郁样行为，通过 qPCR 检测小鼠大脑皮层和血清中 miR-12 的表达。
结果：在造模周和 8 周后，应激组小鼠蔗糖偏好率均显著低于对照组小鼠，不动时间均显著高于对照组小鼠。造模 8 周后，应激小鼠的大脑皮层和血清 miR-12 表达显著上调。结论：出现抑郁样行为的慢性应激小鼠脑内及血清的 miR12表达显著上调。

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Efficacy and prognosis of neonatal severe meconium aspiration syndrome ventilator combined with pulmonary surfactant treatment

Chen Wen-chao, Cheng Hong-bin

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 153-155.

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Objective To evaluate the efficacy and prognosis of neonatal severe meconium aspiration syndrome (MAS) ventilator combined with pulmonary surfactant (PS). Methods According to the random number table method, 112 cases of neonatal severe MAS children admitted to our hospital from May 2015 to June 2017 were divided into control group (56 cases) and study group (56 cases). The patients in the control group were treated with ventilator mechanical ventilation, and the patients in the study group were treated with PS on the basis of treatment in the control group. The ventilator parameters, blood gas analysis, inflammatory factors, comorbidities and outcome were compared and analyzed in both groups. Results The mean airway pressure (MAP), inhalation oxygen volume fraction (FiO2), peak pressure (PIP) and on the machine were lower than those in the control group. The levels of PCO2 and IL-10 in the combined group were higher than those in the control group at 2h, 12h and 24h after treatment, and the levels of oxygen partial pressure (PO2) and IL-8 were lower than those in the control group. There was no significant difference between the two groups in hypoxic - ischemic encephalopathy and intraventricular hemorrhage and mortality. The incidence of ventilator-associated pneumonia (VAP) and pulmonary air leakage in the combined group were lower than those in the control group. Conclusion Neonatal severe MAS with ventilator combined with PS treatment will help shorten the treatment time, improve blood gas analysis, reduce the inflammatory response, reduce the risk of complications and death.

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In patients with pregnancy-induced hypertension disease inhibition C, hypersensitive c-reactive protein and homocysteine levels analysis of the change and its diagnostic value

Yan Dong, Li Yan, Wang Qi

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 75-77.

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Objective To investigate the changes of cystatin C (CysC), hypersensitive C reactive protein (hs-CRP) and homocysteine (Hcy) levels in patients with pregnancy induced hypertension and their diagnostic value. Methods Choose 5 years, 2017 to July 2015 in our hospital treated 120 cases of patients with pregnancy-induced hypertension disease as the observation group, and at the same time choose in our hospital treated 150 cases of normal pregnancy pregnant women as control group. Compare two groups of patients with CysC, hs CRP and Hcy levels and pregnancy outcomes, and Logistic regression analysis. Results The CysC, hs - CRP and Hcy levels of observation group patientsr espectively were (1.68±0.41) mg/L、 (14.58±0.59) mg/L、 (20.65±1.12)μmol/L, the patients were significantly higher than the level of the control group (0.85±0.35) mg/L、 (3.43±0.37) mg/L、 (8.27±0.98)μmol/L, the difference between groups was statistically significant. Patients with severe pregnancy-induced hypertension CysC, hs - CRP and Hcy levels higher than that of patients with mild, moderate, mild gestational hypertension CysC, hs - CRP and Hcy levels higher than that of patients with mild, the comparison group difference was statistically significant. Observation group of patients with premature delivery, fetal distress and the perinatal death accounted for 20.00%, 29.17% and 29.17%, respectively, significantly higher than that of control group patients were 5.33%, 5.33%, 10.00%, diff between groups was statistically significant. Logistic regression analysis, gestational hypertension disease as the dependent variable, independent variable for CysC, hs - CRP and Hcy, the results show that CysC, hs - CRP and Hcy is associated with gestational hypertension disease difference was statistically significant. Conclusion He levels of CysC, hs-CRP and Hcy in patients with pregnancy induced hypertension are increasing with the aggravation of the disease. It is a sensitive index of the disease, and has important clinical diagnostic value.

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Application of DWI and ADC in the diagnosis of benign prostatic hyperplasia and prostate cancer

Wu Qiang, Gui Ya-ping, Wu Deng-long

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 176-179.

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Objective To investigate the application value of diffusion weighted imaging (DWI) and apparent diffusion coefficient (ADC) in the diagnosis of benign prostatic hyperplasia (BPH) and prostate cancer (PCA). Methods 42 cases of patients with PCA confirmed by biopsy or surgical pathology (PCA group) and 38 cases of patients with BPH confirmed by surgery and follow-up (BPH group) in our hospital from May 2011 to June 2016 were enrolled in the study. DWI was performed before operation, and the b value was 800s/mm². The DWI and ADC images of the two groups were observed and compared. The ADC values of non-PCA invading the prostate tissues and BPH tissues were compared, and ADC values of peripheral carcinoma tissues of PCA and the prostate tissues of BPH were also compared. Results In BPH group, the gland hyperplasia and muscle fiber hyperplasia showed inhomogeneous high signal and inhomogeneous low signal on DWI and ADC images. PCA group showed high signal on DWI and low signal on ADC. There were no significant differences between the two groups in ADC values of normal peripheral zone, glandular hyperplasia, interstitial hyperplasia, bladder and obturator muscle. Compared with the ADC value of PAC peripheral zone, the ADC values of the peripheral zone, the central gland hyperplasia and central muscle fiber hyperplasia of BPH group were significantly higher. Conclusion DWI and ADC can provide quantitative information for the diagnosis of prostate cancer. They are convenient and simple to use, and can be used as assistant tools for clinical diagnosis.

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The clinical effect of laparoscopy in patients with distal stage of gastric cancer and the clinical effect of laparoscopic surgery and three years of survival were observed

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 28-31.

Abstract (1698) PDF (10667)

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目的：探讨腹腔镜下或常规开腹行胃癌D2根治术治疗局部进展期胃癌的临床疗效及3年生存情况。方法：选取2014年4月~2017年11月期间我院收治的局部进展期胃癌患者68例进行回顾性分析，根据不同的手术方法分为观察组和对照组，每组34例。观察组患者采用腹腔镜远端胃癌D2根治术治疗；对照组患者采用常规开腹远端胃癌D2根治术治疗。观察并比较两组患者治疗后的手术情况、术后恢复情况、近期治疗效果、并发症发生率及3年生存情况。结果：治疗后观察组在术中出血量、术中输血率、手术切口长度、肛门排气时间、下床活动时间、进流质饮食时间及住院时间方面均明显优于对照组，以上差异均有统计学意义。两组近期治疗效果指标清扫淋巴结数、肿瘤近端切缘和肿瘤远端切缘比较差异无统计学意义。观察组患者治疗后并发症的发生率显著低于对照组，差异有统计学意义。两组患者3年生存率比较无统计学意义。结论：与常规开腹胃癌D2根治术相比，对局部进展期远端胃癌患者腹腔镜下行D2根治术的临床疗效好，患者术中损伤小，术后恢复快，并发症发生率低，而且3年生存率无明显差异，值得在临床上进一步推广应用。

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Relationship between body mass index and absolute renal risk of IgA nephropathy

Lv Wei, Lv Feng, Chen Feng-xiang

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 159-161.

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Objective To investigate the relationship between body mass index (BMI) and absolute renal risk (ARR) in IgA nephropathy. Methods The clinical data of 82 patients with IgA nephropathy admitted in our hospital from January 2012 to August 2017 were retrospectively analyzed. The relationship between BMI and ARR was analyzed. Patients were divided into healthy group (18.5 kg / m2 ≤ BMI <24 kg / m2, 38 cases), overweight group (24 kg / m2 ≤ BMI <28 kg / m2, 28 cases) and obese group (BMI≥28 kg / m2, 16 example). Results There were significant differences in sex, age, diabetes mellitus, hypertension, ARR score, serum uric acid, Scr, BUN and Cys C in healthy group, overweight group and obesity group. There were significant differences in renal vascular score between healthy group, overweight group and obese group. There was no significant difference in glomerular score, renal tubulointerstitial score and total score between the two groups. Multivariate and multivariate logistic regression analysis showed that BMI was an independent risk factor for ARR in patients with IgA nephropathy (OR = 1.423, 95% CI: 1.130 ～ 3.736). Conclusion Obesity is a risk factor for elevated ARR in IgA nephropathy, and obesity may indicate poor prognosis.

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Effects of azithromycin combined with cefodiazine sodium on clinical symptoms and inflammatory status in patients with pulmonary infection

Li Bao-liang, Tong Ping-li

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 146-149.

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Objective Effects of azithromycin combined with cefodiazine sodium on clinical symptoms and inflammatory status in patients with pulmonary infection. Methods Selected 112 cases of pulmonary infection from March 2014 to March 2016, randomly divided into observation group and control group, analysis of the improvement of clinical symptoms and inflammatory status in the two groups. Results After treatment in the observation group and the control group, the remarkable effect was 33(58.93%), 25(44.64%); the effective was 18(32.14%), 17(30.36%); the invalid was 5(8.93%), 14(25.00%); the total effective rate was 51(91.07%), 42(75.00%), there was significant difference. After treatment, there were significant differences in cough and expectoration, acute asthma, fever and lung rale disappearance between the observation group and the control group. After treatment, compared with the control group, the average of NO, SP-A and HMGB1 in the observation group decreased significantly, and the level of ChE increased significantly. After treatment, the serum levels of PAF, TNF-α, IL -6, MIP-1α, CRP and PCT decreased significantly in the observation group compared with the control group. The adverse reactions of the observation group and the control group were nausea and vomiting 2(3.57%), 1(1.79%), abdominal distention and diarrhea 1(1.79%) and 1(1.79%). Conclusion The combination of azithromycin and cefodizime sodium can improve the clinical symptoms of the patient, reduce the inflammatory state, and has good safety.

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Analysis on the prevalence and influence factors of senile cataract of Changsha City

ZHANG Xiao-Mei-1, YANG Jian-Feng-2

HuNan ShiFan DaXue XueBao(YiXueBan). 2015, 12(2): 40.

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Objective To investigate the prevalence and influence factors of Senile cataract in Changsha. Methods Randomly
sampling was used to select 1383 cases from the Database of Physical examination management center of Changsha
Central Hospital. The related influence factors of cataract were analyzed by One-Way ANOVA model and Logistic Regression
model. Results Age, Diabetes, Sun exposing, Long-term smoking are risk factors. Conclusions The Senile cataract is still a
high-occurrence eye disease. The Cataract prevalence rate of persons aged 50 or above in Changsha City is high. The Cataract
is influenced by many factors. We Should actively take preventive measures aimed at influencing factors, to reduce the harm of
senile cataract to the masses.

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Study on the surgerical treatment and outcome of distal humeral coronal shear fractures

YU 　Min, CHOU 　Ke

HuNan ShiFan DaXue XueBao(YiXueBan). 2015, 12(2): 38.

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Objective To investigate the treatment and outcome of distal humeral coronal shear fractures by using Herbert
screw. Methods From October 2010 to September 2013, 8 patients with coronal shear fracture of distal humeral were surgical
treated and fixed with Herbert screws. According to Dubberley classification, type II 3 cases; type III 5 cases. All are Closed
fracture, without Vascular and nerve injury. 3 months after surgery, measure the range of movement and Broberg-Morrey score
of elbow. Result all wound are good healing, pain symptoms disappeared, without infection. The reduction is good, and fracture
are stable, without internal fixation loosening, and no neurological complication. 3 months after surgery, the average range of
movement of elbow is 87.5°; and the average Broberg-Morrey score of is 89, which is excellent. Conclude: To treat the coronal
shear fractures of the distal humerus by using the Herbert screw can obtain ideal surgerical effect such as compressing the
fracture without influence the trail of elbow, satisfied recovery of elbow function.

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Study on treatment of secretory otitis media in patients with nasopharyngeal carcinoma treated by spontaneous eustachian tube blowout and balloon dilatation

Zhang Nan, Peng Xia, Shi Zeng-xia

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 78-80.

Abstract (1686) PDF (11232)

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Objective To investigate the clinical effect of automatic eustachian tube blow + balloon dilatation in the treatment of secretory otitis media in patients with nasopharyngeal carcinoma radiotherapy. Methods 80 patients with nasopharyngeal carcinoma treated with secretory otitis media were enrolled from January 2014 to April 2016. According to the random number table method, the selected subjects were randomly divided into control group (42 cases) and observation group (38 cases). The patients in the control group were treated with tympanostomy. The patients in the observation group were treated with automatic eustachian tube bleed and balloon dilatation. The clinical efficacy of the two groups after 6 months of treatment, the 3-month, 6-month, 12-month Eustachian tube dysfunction score and the Valsalva test score, and the incidence of adverse events were observed. Results The clinical effective rate (94.74%) in the observation group was significantly higher than that in the control group (76.19%), the difference was statistically significant; Two groups of patients after 3 months, 6 months, 12 months were significantly lower than before treatment, the patients in the observation group had the same time ETQR-7 score were significantly lower than those in the control group; The Valsalva score was decreasing in the two groups at 3 months, 6 months, and 12 months, and the observation group was followed by the Valsalva score was significantly lower than that in the control group. The incidence of recurrence and the recurrence rate (7.89%) in the observation group were lower than those in the control group (42.86%), which was statistically significant. Conclusion The treatment of secretory otitis media in patients with nasopharyngeal carcinoma radiotherapy has significant clinical effect, which can effectively improve the Eustachian tube dysfunction and clinical symptoms, low recurrence rate, high safety and wide In clinical application.

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The epidemiological investigation of Chronic kidney disease in Hunan province

Li Yan, Yang Hai-rong, He Yan, et al

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 180-183.

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Objective To investigate the prevalence and risk factors of chronic kidney disease (CKD) among adults in Hunan Province. Methods Using cross-sectional study, take the method of multistage sampling, the survey was performed in part of residents (aged≥18 years) from 5 cities of Hunan Province, data was obtained by questionnaire of risk factors of CKD, physical examination, and laboratory examination. Results The prevalence of chronic kidney disease in Hunan province was 10.81%, it increased with the growth of the age, the awareness rate among CKD population was 20.8%. The prevalence of albuminuria was 1.85%, and the prevalence of albuminuria in males were significantly higher than females, and it increased with age; The prevalence of hematuria was 7.40%, females were higher than males, there was a significant statistical significance about the prevalence of hematuria in different ages. The prevalence of reduced eGFR was 2.65%, There was a significant statistical significance about the prevalence of reduced eGFR both in different genders and ages. Multiple factor logistic regression analysis results show that age, high systolic blood pressure, diabetes were closely related to CKD. Conclusion The prevalence of chronic kidney disease (CKD) in the adult population of Hunan is similar to that in China. Age, high systolic blood pressure, diabetes were closely related to CKD.

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Hunan clinical blood type EQA of analysis and discussion is about the testing results of Rh weak D blood group antigen

Jiao Fang-yan, Yang Li-hua, Peng Ping, et al

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 186-189.

Abstract (1669) PDF (11075)

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Objective To evaluate the detectability of Rh weak (D) blood group antigen in External Quality Assessment (EQA) of Evaluatiaon Laboratory in Hunan province. Methods The indirect anti-human globulin tests anti-D (IgD) are field tested and surveyed by the stuff in Hunan Center For Clinical Laboratory (abbreviate Center), and The red blood cells of Rh weak (D) are tested by each laboratory in a way of microcolumn gel card method and tube method; The red blood cells of Rh weak (D) are tested by EQA Evaluatiaon Laboratory according to the report Clinical Laboratory Quality Assessment Requirements (GB/20032301-T-361) and PT Evaluation Protocols. Results Field testing and survey: The accordance rate of positive weak (D) in anti-human globulin card+anti-D (IgD) in field test and survey are obviously higher than the microcolumn gel card method and tube method ( (anti (IgM) ); 90% of EQA laboratories did not take Rh (D) negative test confirmation again, while 10% of EQA laboratories chose wrong methods and reagents during this process. EQA Evaluatiaon Laboratory: There are 407 laboratories, among of which 12 take Rh (D) positive test confirmation experiment, with only 2.7% in positive accordance rate; there are 60 blood transfusion management and maternal and child care institutions, among of which 2 take Rh (D) positive test confirmation experiment, with only 3.3% in positive accordance rate. The weak D positive accordance rate are lower than the standard with those three methods in EQA Evaluatiaon Laboratory, without any sense in statistics. Conclusion The EQA Evaluatiaon Laboratory has low level in the testing ability of Rh weak (D) blood type . Anti-human globulin test anti-D (IgD) is regarded as Rh (D) negative test confirmation after conventional serologic detection of Rh (D), especially in maternal and child health care institutions and blood transfusion management the confirmation experiments must be putted into effect.

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The value of micro probe endoscopic ultrasonography in diagnosis of colorectal precancerous lesions in analysis

Wu Qi-wei, Shen Rong, Lu Jun, Zhang Li

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 72-75.

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Objective To study the diagnostic value of miniprobe endoscopic ultrasonography in the diagnosis of colorectal precancerous lesions, and provide reference for clinical prevention and treatment. Methods From January 2016 to May 2017 received 85 cases of colorectal polypoid lesions in patients with colonoscopy examination in our hospital as the research object, and were confirmed by pathological diagnosis, there were 157 polyps like lesions. All patients underwent micro probe endoscopic ultrasonography, the pathological diagnosis as the gold standard were analyzed. Results 157 polypoid lesions, 62 hyperplastic / inflammatory polyp, 81 adenoma, 14 of adenocarcinoma; NBI lesions The contour and surface structure, microvascular morphology definition was significantly better than that of ordinary endoscopy, the difference was statistically significant; NICE typing showed that 157 lesions: 61 lesions were type I, 80 lesions were type Ⅱ, 16 lesions were type Ⅲ; sensitivity and specificity of micro probe endoscopic ultrasonography check the large bowel tumor lesions, the accuracy was 94.7%, 90.3%, 93%; stage compared with the postoperative pathological diagnosis, micro probe endoscopic ultrasonography in diagnosis of colon cancer T staging accuracy rate of diagnosis is 85.7%. Conclusion Miniprobe endoscopic ultrasonography in the diagnosis of colorectal precancerous lesions. High value, can effectively improve the detection rate of colorectal precancerous lesions, with high sensitivity and specificity, accuracy, worthy of promotion.

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Analysis of pulmonary function, inflammatory status and clinical treatment in patients with moderate to severe COPD

Zhang Yun-yun, Xie Xiang

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 119-122.

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Objective To investigate the Seretide in the treatment of moderate and severe COPD (Chronic obstructive pulmonary disease) to improve lung function in patients with you, inflammation status and clinical therapeutic effect. Methods 70 cases of patients with severe COPD from December 2015 to December 2016 in our hospital, according to the different treatment methods were divided into two groups, 35 cases in each group, two groups of patients were given conventional treatment, the control group was given the treatment of Theophylline Sustained-release Tablets, on the basis of the observation group patients were given Seretide treatment, observe two groups of patients before and after treatment, lung function, inflammatory factors expression, dyspnea score the changes recorded in patients with clinical efficacy after treatment, and compared. Results After treatment, the pulmonary function indexes such as FEV1, FVC and FEV1/FVC were improved, and the difference between the two groups was statistically significant; the expression levels of inflammatory factors in the treatment of patients before the comparison, no statistically significant; after the treatment, the expression level of serum inflammatory factors in patients with IL-6, CRP and TNF- etc were decreased, the observation group than the control group, the difference was significant after treatment; , dyspnea score of patients were lower than those before treatment, the observation group (1.56+0.32) lower than that of control group (1.98 + 0.42), significant difference; after treatment, the total effective rate was 94.29% higher than 71.43% in the control group, significant difference the adverse reactions of the observation group was 5.71%, which was lower than that of the control group (22.86%), and the difference was significant. Conclusion Given the severe Seretide treatment in patients with COPD, can improve the patient’s lung function, relieve the symptoms of breathing difficulties, inhibit the expression of inflammatory cytokines, significant effect.

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Comparison of the curative effect of different regimens on diabetic peripheral neuropathy

Wen Bao-hua, Shen Shan-mei

HuNan ShiFan DaXue XueBao(YiXueBan).

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Objective To study the curative effect of different regimens on diabetic peripheral neuropathy (DPN). Methods 96 cases ofpatients with DPN treated in our hospital fromOctober 2015 to February 2017 were selected as study subjects, andthey were divided into A, B and C three groups by random number table method, with 32 cases in each group. Group A were treated with mecobalamin on the basis of routine treatment. Based on the treatment for group A, group B were additionally treated with αlipoic acid, and group D were additionally treated with Mudan granules and α lipoic acid. The blood glucose, blood lipids, vibration sense, pressure sense, temperature sense, ankle jerk, symptom score and adverse reactions were compared among the three groups before treatment and after 10 days of treatment. Results After 10daysof treatment, the vibration perceptionthresholds (VPT) of left and right lower limbs of group C ［ (15.24±2.95) V, (15.07±2.75) V］ were significantly lower than those of group A and group B, while VPT of left and right lower limbs of group B ［ (17.39±3.63) V, (17.11±3.21) V］ were significantly lower than those of group A ［ (19.62±3.14) V, (19.35±2.87) V］. The abnormal rates of ankle jerkin group B and group C after 10 days of treatment (18.8% and 15.6%) were significantly lower than that in group A (43.8%). Conclusion Mudan granules combined with α lipoic acid and mecobalamin can promote the repair of damaged nerve tissues andrelieve symptoms of DPN.

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Research on the Prevention of Radiation Lung Injury by Bone Marrow Mesenchymal Stem Cell Transplantation and the Mechanism

Chen Chao-wei,Xie Xiao-xue,Lou Fan,Zhang Rong,Yang Pei,Yang Jing-ru,Jin He-kun

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 19-24.

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目的：探讨骨髓间充质干细胞移植对放射性肺损伤的防治作用及相关机制。方法：80 只雌性 SD 大鼠随机分为 A（单纯干细胞组），B（单纯照射组），C（干细胞治疗组），D（空白对照组）。B、C 组大鼠建立放射性肺损伤模型；A、C 组大鼠经尾静脉注射 1mL BMSCs。第 8、12 周取各组大鼠外周血及肺组织，观察并分析干细胞移植对放射性肺损伤及相关分子表达的影响。结果：TSPY 基因在 C 组大鼠肺组织中的表达显著升高。C 组的肺泡炎性反
应较 B 组轻，各组均未观察到明显的肺纤维化，但免疫组化可见 C 组促纤维化因子 TNF-α、TGF-β1 较 B 组低表
达；C 组 SP-B、Arg-1、SOD 的表达显著高于 B 组，而 iNOS 的表达显著低于 B 组。结论：（1）BMSCs 尾静脉移植可
有效减轻大鼠的放射性肺损伤。（2）TNF-α、TGF-β1、SOD、iNOS、Arg-1 分子表达水平的改变在其过程中起到重要作用。

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Clinical significance of serum tumor markers in predicting the efficacy of targeted drug therapy for advanced non-small cell lung cancer

Xu An-ping

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 129-132.

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Objective To study the tumor markers carcinoembryonic antigen (CEA) and squamous cell carcinoma associated antigen (SCCAg), neuron specific enolase (NSE) in advanced non-small cell lung cancer, in the process of evaluation of the efficacy of clinical application. Methods 78 cases of advanced non-small cell lung cancer patients as the research object, according to the targeted therapy after curative effect is divided into partial remission group 39 cases, 24 cases of stable disease group, 15 cases in group, compared three groups of subjects targeted drug therapy before and after eight weeks of treatment, tumor markers CEA, SCCAg and NSE in serum the level changes of tumor markers in evaluating the therapeutic effect of clinical application. Results The results of different pathological types of non-small cell lung cancer tumor markers: CEA increased statistically in any group of squamous cell carcinoma, squamous adenocarcinoma and adenocarcinoma; SCCAg increased and there was significant difference in any group in adenocarcinoma, squamous adenocarcinoma, squamous cell carcinoma; NSE is the highest in adenocarcinoma, no statistically significant difference in squamous cell carcinoma, squamous adenocarcinoma; partial remission group of patients with non-small cell lung cancer tumor markers CEA, SCCAg, NSE serum levels were decreased, the difference was statistically significant; stable disease group non-small cell cancer tumor markers CEA, SCCAg and NSE serum levels no significant change; partial remission in patients with tumor progression of non-small cell carcinoma markers CEA, SCCAg, NSE serum levels were elevated, the difference was statistically significant. Conclusion The monitoring of tumor markers is a reliable approach to understand the effectiveness of molecular targeted therapies, and is also the basis for molecular targeted drug selection.

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Effect of furosemide, digitalis, canola combined with BIPAP of noninvasive ventilation on COPD with left ventricular failureand pulmonary edema

Li Xue-feng

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 110-113.

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Objective To observe the clinical effect of BIPAP of noninvasive ventilation in the treatment of COPD with left ventricular failure and pulmonary edema on the basis of conventional diuresis, cardiac and central excitatory drugs such as furosemide, digitalis and canola. Methods The clinical data of 135 patients with COPD complicated with left heart failure pulmonary edema from January 1, 2014 to December 31, 2016 in our hospital were retrospectively analyzed, and the patients were divided into observation group (n=68) and control group (n=67) according to different treatment methods. Among them, the control group was treated with furosemide, digitalis, canola and other conventional diuresis, cardiac and central excitatory drugs for treatment, while the observation group added BiPAP treatment based on the control. The general clinical indicators at 2 hours after the treatment, the treatment effect, cardiac function improvement and of lung function improvement at 3 months after the treatment of the two groups were compared. Results Two hours after the treatment, the levels of SBP, SDP, HR, PaO₂, PaCO₂, SaO₂ and Brog dyspnea score in the observation group were significantly better than those in the control group. Three months after the treatment, the total effective rate was 92.65% in the observation group, which was significantly higher than 79.10% in the control group; the cardiac parametersof LVEF, 6 min walking distance and BNP level in the observation group were significantly better than those in the control group, the lung function indexes of FEV₁, FEV₁/FVC and FEV₁% Pred in the observation group were significantly better than those in the control group. Conclusion For patients with COPD complicated with left heart failure and pulmonary edema, BiPAP treatment combined with conventional diuresis, cardiac and central excitatory drugs such as furosemide, digitalis and canola can significantly rapidlyrelieve the clinical symptoms of patients, improve the patient’s heart and lung function, and the treatment effect is better.

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The effect of different anastomotic surgeries on the incidence of complications after pancreaticoduodenectomy

Tang Jun

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 166-169.

Abstract (1615) PDF (10786)

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Objective To investigate the effect of different anastomotic surgeries on the incidence of complications after pancreaticoduodenectomy (PD). Methods The clinical data of 100 patients treated with PD by pancreatic duct jejunal mucosa anastomosis (group A, n=38), encasing pancreatic and intestinal anastomosis (group B, n=32) and pancreatic jejunal bundle anastomosis (group C, n=30) were retrospectively analyzed. The incidence of postoperative complications and the severity of pancreatic fistula were compared. The pancreatic exocrine function indexes at 1 year after surgery ［fecal elastase (FE) ］, endocrine function indexes ［glucagon (GLC), somatostatin (SS) ］, nutritional status indexes ［body mass index (BMI), serum albumin, prealbumin］ and the quality of life score ［Functional Assessment of Cancer Therapy-General (FACT-G) ］ were recorded. Results There was no perioperative death in the three groups. The incidence of postoperative complications showed group C < group A < group B. The incidence of pancreatic fistula and grade C rate were significantly lower in group C than the other two groups, but there was no significant difference between group A and group B.1 year after surgery, there were no significant differences among the three groups in pancreatic exocrine function indexes, pancreatic endocrine function indexes and nutritional status. The FACT-G score showed group C > group A > group B. Conclusion The three anastomotic surgeries are of certain application value in PD, and pancreatic jejunal bundle anastomosis can effectively reduce the risk of postoperative complications, which is good for improving the quality of life of patients.

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Effects of Epidural Anesthesia Combined with General Anesthesia on Hemodynamics and Stress Response in Elderly Patients with Hypertension

Wei Li-xia, Zhou Zhi-chuan

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 122-125.

Abstract (1614) PDF (10853)

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Objective To investigate the effect of epidural anesthesia combined with general anesthesia on hemodynamics and stress status in elderly patients with hypertension. Methods One hundred and eighteen elderly patients with hypertension treated by laparoscopic cholecystectomy from January 2014 to January 2017 were selected, and were randomly divided into group A and group B, fifty-nine cases in each. General anesthesia was used in group A, while epidural anesthesia combined with general anesthesia was used in group B. The systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate (HR) and oxygen saturation (SpO₂) before anesthesia (T0), after intubation (T1), 10min after pneumoperitoneum (T2), and before extubation (T3) were compared between the two groups. The levels of growth hormone (GH), prolactin (PRL) and cortisol (Cor) in T0, T1, T2 and T3 were compared between the two groups. The breathing recovery time, awake time and extubation time after operation were compared between the two groups. The incidence of emergence delirium were compared between the two groups. Results The SBP, DBP and HR of the two groups in T1, T2 and T3 were significantly higher than those in T0. The SBP and DBP of group B in T1 T2 and T3 were significantly lower than those of group A, while the HR was significantly higher than that of group A. The levels of GH, PRL and Cor of group B in T1, T2 and T3 were significantly lower than those of group A. The breathing recovery time, awake time and extubation time after operation of group B were significantly less than those of group A . The incidence rate of emergence delirium of group B was 6.78%, which was significantly lower than that of group A. Conclusion Epidural anesthesia combined with general anesthesia is more conducive to maintaining the stability of hemodynamics in elderly patients with hypertension. It can reduce the stress response and shortening the postoperative recovery time.

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Effects of propofol combined with triptolide of cis benzenesulfonate on muscle relaxation and hemodynamics in patients undergoing laparoscopic cholecystectomy

Tang Yan-bin, Xiao Ji-feng, Wu Wen-hua, et al

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 132-135.

Abstract (1611) PDF (10357)

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Objective To observe the effects of propofol combined with atracurium cis-benzenesulfonate on muscle relaxation and hemodynamics in patients undergoing laparoscopic cholecystectomy (LC). Methods 90 patients with LC were randomly divided into the control group (n=45) and the observation group (n=45) according to the lottery method. Both groups were induced by anesthesia. The control group was given propofol combined with vecuronium anesthesia. The observation group given propofol combined with triptolide sulfonate anesthesia, and other auxiliary anesthesia drugs are the same. The effect of muscle relaxation and tracheal intubation in two groups of LC patients are observed. During after anesthesia (T0), endotracheal intubation (T1), cutaneous (T2), extubation (T3) and extubation (T4), the changes in hemodynamics were measured. Result The onset time and muscle relaxation recovery time of the muscle relaxants in the observation group were significantly lower than those in the control group. The clinical time and the rate of tracheal intubation were significantly higher in the observation group than in the control group, and the difference was statistically significant. At T0, there were no significant differences in the hemodynamic indexes between the two groups. At T1, T2, T3and T4, the systolic blood pressure (SBP), diastolic blood pressure (DBP) and heart rate (HR) of the observation group were significantly lower than those of the control group, and compared with T0 was no significant difference. While the control group SBP, DBP and HR levels was significantly higher than T0, and the difference was statistically significant. Conclusion Acetylcidate sulfonate combined with propofol anesthesia, has good muscle relaxion effect and high level of tracheal intubation assessment, and can stabilize hemodynamics, which is one of the clinical effective LC anesthesia program.

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Effect of Pancreatic Kininogenase Tablets Assisted with Ulinastatin on Microcirculation Disorder in Patients with Acute Pancreatitis

Shu Chang

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 162-165.

Abstract (1608) PDF (10832)

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Objective To observe the effect of pancreatic kininogenase tablets assisted with ulinastatin on microcirculation disorder in patients with acute pancreatitis. Methods 100 cases of patients with acute pancreatitis were randomly divided into the observation group and the control group according to the random number table method, with 50 cases in each group. The patients in the control group were treated with ulinastatin injection of 100, 000 units plus 5% glucose injection and the patients in the observation group were given pancreatic kininogenase on the basis of treatment in the control group, and the course of treatment was 2 weeks. The clinical efficacy of the two groups, the levels of TNF-α, PFA, IL-6, IL-10, whole blood high shear viscosity, whole blood low shear viscosity, plasma viscosity, erythrocyte sedimentation and other hemorheological indexes, thrombin time (TT), prothrombin time (PT), partial activated thrombin time (APTT), D-dimer and other coagulation function and the occurrence of adverse reactions were observed and compared between the two groups before and after treatment. Results The total effective rate of the observation group (94%) was significantly higher than that of the control group (76%). The expression levels of TNF-α, PFA, IL-6 and IL-10 in the two groups were significantly lower than those before treatment, and the expression levels of TNF-α, PAF, IL-6 and IL-10 in the observation group were significantly lower than those in the control group. After treatment, the whole blood high shear viscosity, whole blood low shear viscosity, plasma viscosity and erythrocyte sedimentation indexes were significantly lower than those before treatment, and the hemorheological indexes of the observation group were significantly lower than those in the control group. The coagulation function indexes of TT, PT, APTT and D-dimer in the two groups were significantly longer than those in the control group, and the coagulation function indexes of the observation group was significantly longer than those of the control group. There was no significant difference in the incidence rate of adverse reactions between the two groups. Conclusion Pancreatic kininogenase tables assisted with ulinastatin can effectively improve the clinical symptoms of patients with acute pancreatitis, can dilate the blood vessels by reducing the levels of inflammatory factors and coagulation function to improve microcirculation disorder without increasing the adverse reactions. Therefore it is worthy of clinical promotion and application.

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Analysis of Risk Factors and Risk Assessment of Deep Venous Thrombosis in Patients Undergone Hip Replacement

Xu Feng-yun

HuNan ShiFan DaXue XueBao(YiXueBan).

Abstract (1606) PDF (10595)

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Objective To analyze the risk factors of deep venous thrombosis in patients undergone hip replacement, and to investigate the application value of Autar Scale risk assessment. Methods A total of 101 cases of patients admitted to hospital from January 2015 to December 2016 and undergone hip replacement were selected as the research objects. The methods of single factor and multi-factor logistic regression analysis were used to analyze the risk factors of deep venous thrombosis in those patients, and Autar Scale was used to assess the risk degree of deep venous thrombosis. Results Among the 101 cases of patients, 15 cases had postoperative deep venous thrombosis, with the incidence rate of 14.85%. Single factor analysis showed that the incidence rate of deep venous thrombosis in female patients and patients with complicating diseases (hypertension, diabetes, cardiovascular and cerebrovascular diseases) was significantly higher than that of patients without deep venous thrombosis; the incidence rate of patients with postoperative early activities and postoperative use of anticoagulants was significantly lower than that of patients without deep venous thrombosis; the incidence rate of aged patients was significantly higher than that of patients without deep venous thrombosis. Multi-factor logistic regression analysis showed that female, advanced age, complicated hypertension, diabetes and cardiovascular and cerebrovascular disease are risk factors of deep venous thrombosis after hip replacement, postoperative early activities and postoperative use of anticoagulants are protective factors that prevent deep venous thrombosis; the incidence rate of deep venous thrombosis in high risk patients was significantly higher than that of low risk and medium risk patients; the incidence rate of deep venous thrombosis in medium risk patients was significantly higher than that in low risk patients. Conclusion The incidence rate of deep venous thrombosis in patients undergone hip replacement is high, and it is significantly correlated with gender, age and complicating diseases. The risk assessment on deep venous thrombosis should be strengthened, and predictable and targeted nursing intervention strategies should be proposed to reduce the incidence rate of deep venous thrombosis.

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Study on the curative effect of laparoscopic common bile duct exploration combined with T-tube suture in the treatment of common bile duct stones

Shen Biao1, 2, Song Zhen-shun1

HuNan ShiFan DaXue XueBao(YiXueBan). 2018, 15(3): 69-71.

Abstract (1604)

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Objective To study the efficacy of laparoscopic common bile duct exploration without T-tube suture in the treatment of common bile duct stones. Methods 408 cases of patients with common bile duct stones who were hospitalized from February 2012 to June 2016 were selected as the subjects. All the patients were randomized to the control group and the observation group, each group of 204 cases. The control group received laparoscopic common bile duct exploration (with T-tube suture); and the patients in the observation group were treated with laparoscopic common bile duct exploration without T-tube suture. The operative parameters, postoperative recovery effect and postoperative complications were observed. Results The operative time and intraoperative blood loss were significantly lower in the observation group than in the control group. The primary exhaust time, drainage time and hospital stay were lower in the observation group than those in the control group, the difference was statistically significant. There was no significant difference between the two groups in the bile duct hemorrhage, infection, stenosis and bile leakage. Conclusion In the treatment of common bile duct stones, laparoscopic common bile duct exploration without T-tube suture treatment of surgical trauma, bleeding less, can reduce the risk of postoperative complications, conducive to postoperative recovery as soon as possible, shorten the hospital Time, more with a minimally invasive surgical advantage.

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