Journal of Hunan Normal University(Medical Science)

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SNRPB Modulates Hepatocellular Carcinoma Malignancy via the Hippo Signaling Pathway

HU Xinyue, LOU Lei, DAI Qianlong, PENG Shuchang, GUO Xiaowei

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 1-7.

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Objective To investigate the oncogenic role of small nuclear ribonucleoprotein polypeptides B and B1 (SNRPB) in hepatocellular carcinoma (HCC) and its underlying molecular mechanisms. Methods Multi-omics databases were utilized to analyze the expression and clinical significance of SNRPB in HCC. The effects of SNRPB on HCC cell migration and invasion were evaluated using Western blot and Transwell assay. The molecular mechanisms of SNRPB in regulating the Hippo signaling pathway were investigated through dual-luciferase reporter assay, co-immunoprecipitation (Co-IP), and ubiquitination assays. Results SNRPB was highly expressed in HCC tissues and cell lines, and its expression was significantly associated with poor prognosis in patients. Functional experiments demonstrated that SNRPB promoted HCC cell migration and invasion. Mechanistic studies revealed that SNRPB interacted with YAP protein, inhibited its ubiquitination-mediated degradation, and thereby stabilized YAP protein, enhancing the transcriptional activity of the downstream target gene CTGF. Furthermore, YAP positively regulated SNRPB expression, forming a SNRPB-YAP positive feedback loop. Conclusion SNRPB promotes HCC progression by modulating the Hippo signaling pathway, offering a novel potential therapeutic target for HCC treatment.

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The Regulatory Role of RTN4 in Proliferation, Apoptosis, and Migration of Cervical Cancer Cells

ZOU Zhi, LI Yike, LIU Bang, LIANG Yun, LI Lulu, SHI Yan

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 8-16.

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Objective To investigate the effects of RTN4 on the proliferation, apoptosis, and migration of HeLa cells, providing a new theoretical basis for the molecular mechanisms and therapeutic targets in the development of cervical cancer. Methods The expression differences of RTN4 mRNA and protein in cervical cancer and adjacent tissues were identified through databases such as TCGA, GTEx, GEO, and HPA, as well as immunohistochemical staining. After upregulating and downregulating RTN4 levels in HeLa cells, the effects of RTN4 on cell proliferation, apoptosis, and migration were observed using qPCR, Western Blotting, CCK-8 assay, colony formation assay, scratch assay, invasion assay, and apoptosis assays. Results Database analysis revealed that the levels of RTN4 mRNA and protein were significantly decreased in cervical cancer tissues compared to adjacent tissues. Immunohistochemical staining results showed that RTN4 protein was significantly underexpressed in cervical cancer compared to adjacent tissues. In HeLa cells, upregulating RTN4 levels led to a significant decrease in cell proliferation, migration, and invasion abilities, along with a significant increase in apoptosis rate. Conversely, downregulating RTN4 levels resulted in a significant increase in cell proliferation, migration, and invasion abilities, while the apoptosis rate significantly decreased. Conclusion The expression of RTN4 mRNA and protein in cervical cancer tissues is significantly lower than that in adjacent tissues. In cervical cancer cells, overexpression of RTN4 can inhibit cell proliferation, migration, and invasion, while promoting apoptosis; downregulation of RTN4 exhibits the opposite phenotype. These results suggest that RTN4 may serve as a new target for targeted therapy in cervical cancer.

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Activation of Nrf2/HO-1 Pathway by Dexmedetomidine Alleviates Oxidative Stress and Inflammatory Response in Lipopolysaccharide Induced Acute Lung Injury Rats

CHENG Long, ZHAO Shuchuan, CUI Jianhua, LI Jiangjiang, FENG Qian, WANG Jing

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 17-22.

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Objective To investigate the mechanism of dexmedetomidine regulating the nuclear factor erythroid 2-related factor 2/heme oxygenase-1(Nrf2/HO-1) signaling pathway on oxidative stress and inflammatory response in lipopolysaccharide-induced acute lung injury (ALI). Methods Mice were randomly divided into a blank control group (Control group), ALI mouse model group (ALI group), dexmedetomidine intervention group (DEX group), and dexmedetomidine combined with Nrf2 inhibitor ML385 group (DEX+ML385 group). Calculate the wet/dry weight ratio of lung tissue, detect pathological changes in lung tissue using HE staining, detect oxidative stress indicators in lung tissue and levels of inflammatory factors in alveolar lavage fluid, and detect the expression of Nrf2 and HO-1 proteins in lung tissue using Western blotting. Results Compared with the control group, mice in the ALI group showed significantly higher lung tissue wet/dry weight ratio (W/D), lung injury pathological score, counts of total cells, neutrophils and macrophages in bronchoalveolar lavage fluid (BALF), levels of tumor necrosis factor-α (TNF-α), interleukin (IL) -6, IL-1β and IL-18, as well as malondialdehyde (MDA) content and myeloperoxidase (MPO) activity in lung tissue. In contrast, glutathione peroxidase (GSH-Px) content, superoxide dismutase (SOD) activity, and protein expressions of nuclear factor erythroid 2-related factor 2(Nrf2) and heme oxygenase-1(HO-1) in lung tissue were markedly lower in the ALI group. Compared with the ALI group, the DEX group showed significantly decreased lung tissue W/D value, lung injury histopathological score, total cell count, neutrophil count, macrophage count, TNF-α, IL-6, IL-1β, IL-18 levels in BALF, lung tissue MDA content and MPO activity. Moreover, the DEX group exhibited significantly increased lung tissue GSH-Px content, SOD activity, Nrf2, and HO-1 protein expression. However, compared with the DEX group, the DEX AL385 group showed significantly increased lung tissue W/D value, lung injury histopathological score, total cell count, neutrophil count, macrophage count, TNF-α, IL-6, IL-1β, IL-18 levels in BALF, lung tissue MDA content and MPO activity. Additionally, the DEX AL385 group exhibited significantly decreased lung tissue GSH-Px content, SOD activity, Nrf2, and HO-1 protein expression (P<0.05). Conclusion Dexmetomidine can improve the antioxidant capacity and inhibit inflammatory response in lipopolysaccharide-induced acute lung injury mice, thereby exerting a protective effect on lung tissue. Its mechanism of action may be related to the activation of the Nrf2/HO-1 signaling pathway.

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Spermidine Inhibits Pathological Extracellular Matrix Remodeling in Lung Tissue of Acute Lung Injury (ALI) Mice via the Nrf2-Keap1 Axis

HUANG Zhong'en, SUN Ziyan, LI Zhouzhou, ZHU Xinyi, JIANG Chunjiang, LI Jiayi, SUN Xing, WEI Yunfu, SUN Guoying

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 23-28.

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Objective To investigate the effect and regulatory mechanism of spermidine on extracellular matrix (ECM) remodeling in the lung tissue of acute lung injury (ALI) mice. Method An ALI mouse model was constructed. Masson staining was used to observe collagen deposition in lung tissue. Real-time PCR and Western blot were employed to detect the expression of nuclear factor erythroid 2-related factor 2(Nrf2), Keap1, HO-1, PI3K, p-PI3K, MEK1, p-ERK1/2, MMP2, MMP9, TGF-β, TIMP-1, Smad2/3, and collagen I mRNA and protein in lung tissue and macrophages, respectively. Results (1) Masson staining results showed that compared with the spermidine+ALI group, the collagen deposition in the lung tissue of ALI mice pretreated with an Nrf2 agonist was significantly reduced. PCR results demonstrated that spermidine could reduce the expression of Mmp2, Mmp9, and Tgf-β mRNA in ALI lung tissue while increasing Timp-1 mRNA expression. After pretreatment with Nrf2 inhibitors, the expression of Mmp2, Mmp9, and Tgf-β mRNA in the lung tissue of ALI mice increased, whereas Timp-1 mRNA expression decreased. The use of Nrf2 agonists reduced Mmp9 mRNA expression in lung tissue and increased TIMP-1 mRNA expression. (2) Spermidine was found to increase PI3K and MEK protein levels in lung tissue during ALI. In LPS-induced macrophages, spermidine increased the protein expression of PI3K, p-PI3K, MEK1, and p-ERK1/2. Compared with the spermidine+LPS group, the use of PI3K inhibitors resulted in decreased Nrf2 expression, increased Keap1 expression, and decreased HO-1 expression in LPS-induced macrophages pretreated with spermidine. Furthermore, the use of MAPK inhibitors attenuated the LPS-induced upregulation of Nrf2 and HO-1 expression in macrophages, accompanied by decreased Keap1 expression. Conclusion Spermidine, through PI3K and MAPK signaling pathways, activates the Nrf2-Keap1 functional axis, thereby inhibiting macrophage activation, leading to decreased matrix production, ultimately inhibiting pathological ECM remodeling in the lung tissue of ALI mice.

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Construction of a New Molecular Typing and Scoring System for Risk at S/G2 Phase between Different Prognostic Risks and Treatment Responsiveness in Low-Grade Gliomas

LIU Qiao, LIU Jie, FANG Jianing, GAN Xia, ZHOU Yongzhu, YU Xing, LIU Huai, WANG Hui

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 29-45.

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Objective The major factors contributing to poor prognosis and treatment outcome in low grade glioma (LGG) include recurrence and malignant progression. Preventing cancer cells from prematurely entering the G2 stage from the S stage is crucial for tumor invasion and metastasis, and its molecular mechanisms still have research gaps in LGG. To investigate the predictive value of molecular isoforms based on risk crossing at S/G2 phase (RASG2) related genes on the prognosis and treatment responsiveness of LGG patients. Methods We downloaded data from 509 samples from the TCGA-LGG cohort, performed cluster analysis to identify different subtypes based on seven RASG2-related genes, and assessed subtype differences in clinicopathological and biological features. Estimation and single-sample gene set enrichment analyses were used to parse the tumor immune microenvironment (TIME) for LGG. A predictive scoring system was created using Cox regression analysis, Least Absolute Shrinkage with Selection Operator regression, and the Random Forest algorithm. This scoring system was used to categorize LGG patients into a high RASG2S (risk crossing at S/G2 phase score) group and a low RASG2S group. The association of RASG2S with the mutation status, prognosis, and treatment responsiveness of LGG patients was evaluated by mutation analysis, survival analysis, and drug sensitivity analysis based on the TCGA and CGGA cohorts. The differential expression of the RASG2 gene, centered on TICRR and MTBP, between LGG cells and normal cells, and its effect on the proliferation and invasive ability of LGG were verified by cellular experiments. Results All LGG patients were categorized into two subtypes, A and B, based on the expression profiles of seven RASG2-related genes. Patients with the two subtypes had distinct prognosis and TIME infiltration characteristics. Further constructed RASG2S independently predicted the prognosis and treatment responsiveness of LGG, and patients in the low RASG2S group had a significantly better prognosis, with a higher proportion of IDH mutations, 1p19q co-deletions, but with lower TIME immune cell infiltration, tumor mutational load and higher temozolomide sensitivity. Conclusion This study reveals a novel RASG2-associated gene signature that can be used to predict prognosis and treatment responsiveness in patients with LGG, which may contribute to clinical decision-making for precision treatment of LGG.

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Construction of a Nomogram Prediction Model for Post-Radiotherapy Nasopharyngeal Necrosis in Nasopharyngeal Carcinoma Based on MRI Imaging Biomarkers and Clinical Features

LU Jingcheng, LI Shirong, TU Yumei

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 46-51.

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Objective To construct a nomogram prediction model for nasopharyngeal necrosis after radiotherapy (PRNN) in patients with nasopharyngeal cancer (NPC) based on MRI radiomics and clinical features. Methods 243 cases of NPC from Meizhou People's Hospital from January 2017 to October 2023 were selected as the research subjects. They were divided into the training set (n=194) and the test set (n=49) in a ratio of 8∶2. All NPC patients received radiotherapy. The training set was divided into the PRNN group (n=57) and the non-PRNN group (n=137) based on whether the patients presented with PRNN. By comparing the clinical data of the two groups of patients in the training set, LASSO regression was used to screen the MRI imaging features, and an imaging feature score was constructed. Nomogram prediction models for the training set and test set were constructed. Multivariate logistic regression analysis was conducted to identify the influencing factors of PRNN occurrence in NPC patients. Nomogram prediction models for the training set and test set were constructed. Evaluate the performance of the prediction model using receiver operating characteristic curve (ROC), calibration curve, and decision curve analysis (DCA) curve. Result There were statistically significant differences in age, body mass index (BMI), AJCC-T stage, and the second course of radiotherapy between the non-PRNN group and the PRNN group. LASSO regression was used to screen for 35 radiomics features, and the radiomics score was calculated. Multivariate Logistic regression showed that age, BMI, AJCC-T stage, second course of radiotherapy, and radiomics score were the influencing factors for PRNN in NPC patients. Age, BMI, AJCC-T stage, second course of radiotherapy, and radiomics score were used as the clinical model, and the radiomics score as the radiomics model. Age, BMI, AJCC-T stage, second course of radiotherapy, and radiomics score were used as the combined model to construct a nomogram prediction model. ROC curve, calibration curve, and DCA curve showed that the combined prediction model in the test set and training set had significant clinical net benefits and good predictive efficacy. Conclusion Age, BMI, AJCC-T stage, second course of radiotherapy, and radiomics score were the influencing factors for PRNN in NPC patients. The nomogram prediction model constructed based on the above factors had good predictive efficacy and clinical applicability.

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Analysis of the Characteristics of Pathogens and Influencing Factors of Surgical Site Infections in Patients with Thoracolumbar Fractures after Surgery

CHEN Yating, LI Peng, HUANG Xiao, FAN Xiaochun, TANG Jing, LIU Yan

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 52-57.

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Objective To investigate the characteristics of pathogens and influencing factors of surgical site infections (SSI) in patients with thoracolumbar fractures after surgery. Methods A total of 1500 patients with thoracolumbar fractures who underwent internal fixation at Nanchong Hospital of Traditional Chinese Medicine from February 2022 to February 2025 were studied. Patients were divided into non - infection (n=1476) and infection (n=24) groups. Pathogen cultures from SSI patients were analyzed, and clinical data were collected for univariate and multivariate logistic regression analyses. Results Among the 24 patients with SSIs, a total of 28 pathogens were isolated, including 11 Gram-positive bacteria, 16 Gram-negative bacteria, and 1 fungus. The resistance rates of Staphylococcus aureus to amoxicillin, ampicillin, and penicillin were 60.00%, 60.00%, and 80.00%, respectively. The resistance rates of Staphylococcus epidermidis to amoxicillin, ampicillin, and penicillin were 50.00%, 50.00%, and 75.00%, respectively. Escherichia coli exhibited high resistance rates to amoxicillin, ampicillin, piperacillin, and gentamicin, at 50.00%, 66.67%, 83.33%, and 50.00%, respectively. Pseudomonas aeruginosa showed high resistance to co-trimoxazole, amoxicillin, ampicillin, and piperacillin, with resistance rates of 60.00%, 60.00%, 60.00%, and 80.00%, respectively. The infection group had a higher proportion of patients with surgical segments>3, intraoperative blood loss>400 mL, and postoperative drainage tube placement>3 days than the non - infection group. Albumin (ALB) levels were lower in the infection group. Surgical segments, intraoperative blood loss, and postoperative drainage time were positively correlated with SSIs, while ALB levels were negatively correlated. Multivariate analysis revealed intraoperative blood loss, postoperative drainage time, and ALB levels as independent risk factors for SSIs, with intraoperative blood loss showing good predictive value (AUC=0.765>0.700). Conclusion In patients with thoracolumbar fractures, Gram-negative bacteria account for a slightly higher proportion of surgical site infections. Intraoperative blood loss, postoperative drainage time, and ALB levels are independent risk factors for SSIs. Intraoperative blood loss has good predictive value and can serve as a potential biomarker for the early diagnosis of SSI.

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Ultrasound-Guided Minimally Invasive Treatment of Lumbar Disc Herniation in High-Altitude Regions and Changes in Inflammatory Factor Expression

CHEN Yongjun, OUYANG Jianfeng, JIAYANG Zhaxi, WU Bin, REN Li

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 58-62.

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Objective To investigate the clinical efficacy of ultrasound-guided paravertebral block (UG-PVB) and its regulatory effects on inflammatory factors, including Prostaglandin E2(PGE2), 5-Hydroxytryptamine (5-HT), and Cortisol (Cor), in patients with Lumbar Disc Herniation (LDH) in high-altitude regions. Methods A total of 120 patients with LDH admitted to a hospital in Milin County, Tibet, were enrolled and randomly assigned into an experimental group (n=60) and a control group (n=60) using a random number table. The control group received conventional pharmacological treatment, while the experimental group received additional UG-PVB therapy. Blood levels of PGE2, 5-HT, and Cor were measured before and after treatment in both groups. Visual Analog Scale (VAS), Japanese Orthopaedic Association (JOA) score, and Oswestry Disability Index (ODI) were also evaluated. Results After treatment, the experimental group showed significantly lower VAS scores (3.1±1.2 vs 4.7±1.3) and ODI scores (27.3±5.9 vs 35.9±6.1), and higher JOA scores (22.6±3.5 vs 17.8±3.2) compared to the control group (P<0.001). Reductions in inflammatory markers were more pronounced in the experimental group (ΔPGE2=145.5±68.2 vs 87.3±60.6; Δ5-HT=78.5±42.0 vs 39.6±31.4; ΔCor=144.1±58.9 vs -77.4±56.9, P<0.001). Multiple regression analysis indicated that changes in PGE2 and 5-HT were independently associated with VAS improvement (β=-0.42 and -0.38, respectively; P<0.05). Conclusion UG-PVB is effective in relieving pain caused by LDH in high-altitude environments, potentially through suppression of the PGE2/5-HT-mediated inflammatory pathway.

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Analgesic Effect of Ultrasound-Guided Bilateral Quadratus Lumborum Block and its Influences on Opioid Dosage, Postoperative Recovery and Comfort in Open Radical Operation of Ovarian Cancer under General Anesthesia

WANG Yaqi, ZHU Yingping, XU Hui

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 63-67.

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Objective To explore the analgesic effect of ultrasound-guided bilateral anterior quadratus lumborum block (QLB) and its influences on opioid dosage, postoperative recovery and comfort in open radical operation of ovarian cancer under general anesthesia. Methods A retrospective collection was performed on the data from 80 patients with ovarian cancer who underwent radical surgery in the hospital between November 2023 and December 2024. According to different clinical treatment methods, they were divided into control group[40 cases, routine general anesthesia (GA) ]and observation group (40 cases, bilateral anterior QLB+GA). After surgery, all patients were given patient-controlled intravenous analgesia (PCIA). PCIA use, analgesic effect at 4h, 8h, 12h and 24h after surgery, perioperative indexes and inflammatory factors [C-reactive protein (CRP), interleukin-6(IL-6), interleukin-8(IL-8)] before and after surgery were compared between the two groups, and postoperative adverse reactions were recorded. Results Compared with control group, PCIA frequency was lower, postoperative recovery time, extubation time and the first exhaust time were shorter, intraoperative and postoperative dosages of sufentanil were lower, and effective analgesia time was longer in observation group. At 4h, 8h, 12h and 24h after surgery, VAS scores in observation group were lower than those in control group . After surgery, levels of CRP, IL-6 and IL-8 was significantly increased in both groups, which were lower in observation group than control group. The difference in total incidence of adverse reactions between the two groups was not statistically significant. Conclusion The analgesic effect of bilateral anterior QLB is good in open radical operation of ovarian cancer under general anesthesia, which can reduce opioid dosage, relieve inflammatory stress response and promote faster postoperative recovery, with good safety.

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Dynamic Monitoring and Evaluation of Volume Responsiveness and Prognosis in Patients with Septic Shock Based on NT-proBNP and Inferior Vena Cava Variability

FU Jun, LUO Yuhong, SUN Yuewen, WU Hairong, WU Xuejiang

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 68-73.

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Objective To investigate the dynamic assessment value of N-terminal pro-brain natriuretic peptide (NT-proBNP) combined with inferior vena cava variability (IVC-RVI) for volume responsiveness and prognosis in patients with septic shock. Methods A retrospective analysis of 50 patients with septic shock admitted to the Department of Emergency, Wuxi Hospital of Traditional Chinese Medicine. All patients were treated with fluid resuscitation and divided into volume-responsive group (38 cases) and non-responsive group (12 cases) based on their volume responsiveness after treatment; and into survival group (34 cases) and death group (16 cases) based on their clinical outcomes after 28 days. Levels of NT-proBNP and IVC-RVI were measured before treatment and at 24 h and 72 h post-treatment; ROC curves were plotted to analyze the dynamic diagnostic value of NT-proBNP and IVC-RVI in volume responsiveness and clinical prognosis in patients with septic shock. Results Compared with the non-responsive group, the volume-responsive group had lower NT-proBNP levels and higher IVC-RVI levels before treatment, and the combined detection of NT-proBNP and IVC-RVI before treatment had a higher diagnostic value for volume responsiveness (AUC=0.798) than single indicators NT-proBNP (AUC=0.765) and IVC-RVI (AUC=0.770); Compared with the death group, the survival group had lower NT-proBNP levels and higher IVC-RVI levels before treatment and at 24 h and 72 h post-treatment, and the combined detection of NT-proBNP and IVC-RVI before treatment and at 24 h and 72 h post-treatment had a higher diagnostic value for clinical prognosis than single indicators, with the highest diagnostic value at 72 h post-treatment (AUC=0.972). Conclusion Low levels of NT-proBNP coupled with elevated IVC-RVI characterize septic shock patients with favorable volume responsiveness and improved clinical outcomes, while their integrated application demonstrates superior diagnostic performance.

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Risk Factors and Predictive Model Construction of Recurrent Plastic Bronchitis in Children under 12 Years Old

MA Xiaopeng, TAN Xiaohong, LIU Tian, LI Xing zhen, XIE Kunxia, HAO Tingting

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 74-78.

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Objective To investigate the risk factors of recurrent plastic bronchitis in children under 12 years old and further construct a clinical prediction model to provide more reference for early identification of children at high risk of recurrence and follow-up diagnosis and treatment. Methods 111 children with plastic bronchitis diagnosed and treated in our hospital from January 2016 to January 2024 were retrospectively included and grouped according to whether children had recurrence or not after 8 weeks of follow-up. Univariate and multivariate analysis of the risk factors of recurrent plastic bronchitis in children under 12 years old. Construction of a risk prediction model for recurrence of plastic bronchitis in children under 12 years of age and analysis of its predictive efficacy. Results In this study, 38 of 111 children with plastic bronchitis relapsed, with a recurrence rate of 34.23%. The results of univariate analysis showed that the cumulative number of pulmonary lesions, the need for invasive ventilation after plastic removal, and the combination of multiple organ function injury were related to the recurrence of plastic bronchitis in children under 12 years old. Logistic multivariate analysis results confirmed that the number of lung lobe involved, whether invasive ventilation was required after plastic removal, and whether multiple organ function injury combined with extra pulmonary injury were independent factors influencing the recurrence risk of plastic bronchitis in children under 12 years old (P<0.05). The independent influencing factors in logistic regression model and the prediction probability of the regression model were used to predict the recurrence of plastic bronchitis in children under 12 years old. The area under the curve was 0.693, 0.669, 0.702 and 0.849, respectively. Conclusion The recurrence of plastic bronchitis in children under 12 years old may be related to the number of lung lobe involved, whether invasive ventilation is required for the first plastic removal, and whether it is complicated with extra pulmonary multiple organ function injury. The data model constructed by using the above three factors has shown good efficacy in predicting the recurrence of children, which is worthy of further analysis in our work.

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Study on the Fatigue Development Trajectory and Influencing Factors of Patients after Cardiac Valve Replacement Based on the Latent Variable Growth Mixture Model

ZHOU Hao, GUO Yulin, HE Chunyue, JIA Qunmei

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 79-85.

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Objective To explore the types and influencing factors of postoperative fatigue development trajectories in patients undergoing cardiac valve replacement. Methods A questionnaire survey was conducted among 264 patients who underwent heart valve replacement surgery in our hospital from August 2023 to July 2024 using the convenience sampling method. The instruments included the general information questionnaire, the Chinese version of the Multidimensional Fatigue Scale, the Numerical Rating Scale for Pain, the Hamilton Anxiety and Depression Scale, and the Pittsburgh Sleep Quality Index Scale. The fatigue degree of the patients was evaluated by using the Chinese version of the Multidimensional Fatigue Scale at the 1st, 2nd, 3rd, 4th, 5th and 6th months of follow-up respectively. The postoperative fatigue development trajectory of patients undergoing heart valve replacement was identified through the latent variable growth mixture model, and the influencing factors were analyzed by logistic regression. Results A total of 216 patients completed 6 follow-ups. There were three categories of postoperative fatigue development trajectories in patients undergoing heart valve replacement, namely stable fatigue (32.87%), elevated fatigue (16.67%), and decreased fatigue (50.46%). The results of univariate analysis indicated that there were statistically significant differences among the three potential categories of postoperative fatigue development trajectories in patients undergoing heart valve replacement in terms of age, cardiac function classification, pain, anxiety, depression, and sleep disorders. The results of logistic regression analysis indicated that cardiac function classification, anxiety, depression, and sleep disorders were the influencing factors of the category of postoperative fatigue development trajectory in patients undergoing heart valve replacement. Conclusion There is heterogeneity in the postoperative fatigue development trajectory of patients undergoing heart valve replacement. Cardiac function classification, anxiety, depression, and sleep disorders are important influencing factors for the postoperative fatigue development trajectory of patients undergoing heart valve replacement.

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Latent Profile Analysis of Depression and Anxiety Symptoms in Adult Diabetic Patients

LI Na, YE Ying, WANG Honglin, JIN Songna, ZHOU Qiuhong

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 86-92.

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Objective To explore the potential categories and influencing factors of depression and anxiety symptoms in adult diabetic patients. Methods A convenience sampling method was used to select 505 adult diabetic patients from 15 hospitals in Changsha, Hunan Province, based on inclusion and exclusion criteria. Data were collected through a self-designed general information questionnaire, Patient Health Questionnaire, and Generalized Anxiety Disorder. Mplus 8.3 software was used for potential profile analysis, and the potential categories of depression and anxiety symptoms of patients were explored in combination with relevant indicators. SPSS 27.0 software was used to conduct chi square test and multivariate logistic regression to analyze the influencing factors of different categories. Results Depression and anxiety symptoms in adult diabetic patients can be classified into three potential categories: mild depression and anxiety group (C1, 61.0%), moderate depression and anxiety with somatic symptoms group (C2, 30.5%), and severe depression and anxiety group (C3, 8.5%). Logistic regression analysis showed that taking group C1 as the reference group, female patients (OR=1.596, 95% CI: 1.063-2.399) were more likely to belong to group C2; patients living alone (OR=2.964, 95% CI: 1.375-6.390) were more likely to belong to group C3; patients with complications (OR=0.362, 95% CI: 0.169-0.744) and family monthly income>5 000 yuan (OR=0.300, 95% CI: 0.123-0.734) were less likely to belong to group C3. Conclusion Depression and anxiety symptoms in adult diabetic patients can be classified into three potential categories: mild depression and anxiety group: moderate depression and anxiety with somatic symptoms group, and severe depression and anxiety group, and were affected by gender, whether living alone, family monthly income, and complications. Medical staff should develop targeted psychological interventions and health guidance for diabetic patients based on their specific depression and anxiety subgroups.

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Therapeutic Effect of Umbilical Cord Blood Single Nucleated Cells on Partial Rotator Cuff Tears in Rabbits

CHEN Xintong, LI Guanbo, WANG Jing

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 93-99.

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Objective The effects of umbilical blood mononuclear cells (UCB-MNCs) on the biomechanical properties, histological characteristics and molecular mechanism of partial rotator cuff tear healing in rabbits were systematically evaluated to provide an experimental basis for clinical transformation. Methods Thirty New Zealand male rabbits were randomly divided into four groups: blank group (no intervention), cell injection group (UCB-MNCs local injection), control group (modeling + normal saline injection) and experimental group (modeling +UCB-MNCs injection). At 8 and 12 weeks after surgery, the repair effect was analyzed by gross observation, biomechanical tests (maximum tensile load, elastic modulus), histopathological staining (HE, Masson, saffranine solid green), and immunohistochemistry (type I/III collagen), and the safety of UCB-MNCs was evaluated. Results After 8 weeks, scar area of the experimental group was reduced by 42% (P<0.01), and biomechanical strength was significantly increased (82.83±8.22N vs 64.26±2.46 N, P=0.002). At 12 weeks after surgery, the arrangement regularity of collagen fibers in the experimental group reached 78% of normal tendons, and the proportion of type I collagen increased from 35% to 62% (P<0.05), and there was no statistical difference in the biomechanical properties between the experimental group and the blank group (P=0.213). Histology showed that UCB-MNCs significantly inhibited the expression of inflammatory factors such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and promoted the regeneration of fibrocartilage interface. Conclusion UCB-MNCs significantly improved the healing quality of partial rotator cuff tears by inhibiting inflammatory microenvironment and promoting extracellular matrix remodeling, providing experimental basis for non-surgical treatment.

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Research on Small Supernumerary Marker Chromosome (sSMC) in One Genealogy

YI Jianhua, HE Caimei, CHEN Xiaoyan, WEN Hao, ZHOU Zan, LI Hongjun, CHEN Yan, HE Fang, HUANG Xianghong

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 100-104.

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Objective To examine marker chromosomes' impact on male infertility. Methods Peripheral blood lymphocytes were obtained from the proband and infertile couples, and subsequently cultured in a lymphocyte culture medium. After three days of incubation, the cells were harvested, slides prepared, and G-banding staining performed as per standard cytogenetic protocols. Twenty distinct splitting phases were observed under the SNP array, of which five were selected for further analysis. Gene chip tests were conducted on patients with pre-existing conditions and their family members. Results The patient was a 35 year old man with a normal phenotype, married for 7 years, with a normal sexual life, no contraception use, and no children. The semen analysis indicates severe asthenospermia. The Karyotype analysis of peripheral blood chromosomes showed 47 chromosomes, indicating a potential sSMC carrier. Using chip examination technology and the chromosomes of the patient's parents as a control, further research revealed that the patient with primary infertility had a karyotype of 47, X, Yqh-, +mar mat. It was determined that the patient carried an sSMC inherited maternally from the mother. Conclusion The patient in this study inherited the marker chromosome maternally, which may contribute to his severe asthenospermia and infertility. Diagnosis is more accurate with chromosomal examination, karyotype analysis, and comparison of parental chromosomes.

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Clinical Efficacy of Modified Sijun Wupi Decoction Combined with Spironolactone in Patients with Liver Cirrhosis Ascites

MAO Tingyu, YU Xinlei, MA Hongjun, PENG Wei, FAN Jianhu, ZHANG Qian

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 105-109.

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Objective To investigate the clinical efficacy of the addition and subtraction of Sijun Wu Pi Drink combined with spironolactone in patients with cirrhotic ascites. Methods A total of 100 patients with cirrhosis ascites treated in Hunan Combined Traditional Chinese and Western Medicine Hospital between May 2021 and May 2024 were selected. They were divided into two groups, the control group (n=50) and the observation group (n=50) by a random number table method. The control group was given spironolactone treatment, and the observation group was added with Sijun Wupi Drink plus reduction on the basis of the control group. Observe and record the clinical efficacy, ascites subsidence and liver function indexes of the two groups. Results Markedly reduced abdominal circumferences and ascites depths were observed in the observation group, alongside a superior total ascites resolution rate of 98.00%, significantly surpassing the control group's 86.00%. Symptom scores for jaundice (manifesting as yellowing of the eyes or skin), nausea, vomiting, anorexia, epigastric fullness, and abdominal distension, along with levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin (TBil), were considerably lower in the observation group after treatment. In contrast, the albumin (ALB) level was significantly elevated in the observation group. Renal function evaluations revealed that both serum creatinine (Scr) and blood urea nitrogen (BUN) levels remained within normal limits before and after treatment in both cohorts, showing no statistically significant variations within or across groups. Throughout the treatment course, the incidence rates of adverse reactions exhibited no statistically significant difference between the two groups (z=0.088, P=0.711). Conclusion The modified Sijun Wupi Decoction combined with spironolactone can effectively promote the resolution of ascites in liver cirrhosis patients, improve liver function and coagulation parameters, with no significant adverse effects on renal function, demonstrating good safety and clinical applicability.

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Mendelian Randomization of Bronchiectasis with Modifiable Risk Factors Mediated by Gastroesophageal Reflux

HU Tian, LI Wenshen, YI Shanghui, LIU Ying

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 110-117.

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Objectives To elucidate the causal relationship between gastroesophageal reflux disease (GERD) and bronchiectasis (BE), studies were analyzed using a variety of Mendelian randomization methods and to assess the potential mediating role of modifiable risk factors for bronchiectasis in the pathogenesis. Methods Pooled data on GERD and BE were obtained from publicly available genome-wide association studies (GWAS). Single nucleotide polymorphisms (SNPs) were utilized as instrumental variables. Five complementary MR methods were employed, including inverse variance weighting (IVW), MR-Egger, weighted median, weighted mode, and simple mode methods. IVW was the primary method. Additionally, sensitivity analyses, such as Cochran's Q test, MR-Egger intercept test, and leave-one-out analysis, were conducted to assess the robustness of the findings. Two-sample univariate Mendelian randomization and two-step Mendelian randomization analyses were performed to evaluate the causal relationship between GERD and BE. Results Based on the inverse variance-weighted (IVW) analysis, we concluded that there is a significant causal relationship between GERD and BE (OR=1.25, 95% CI: 1.02-1.52). Further mediation analysis indicated that eosinophils and rheumatoid arthritis mediate 4.9% and 50.4% of the effect of GERD on BE, respectively. Sensitivity analyses confirmed the robustness of this causal estimation. Conclusions There exists a causal relationship between genetically predicted GERD and the risk of bronchiectasis, which is partially mediated by rheumatoid arthritis and eosinophil activity.

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Causal Relationship between Relative Sugar Intake and Breast Cancer: A Mendelian Randomization Study

HU Yaxuan, HE Yemei, LIU Xiumei, LIAO Shiyi, XIA Qingqing, CHEN Ouying

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 118-122.

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Objective To analyze the causal relationship between relative sugar intake and breast cancer using Mendelian randomization (MR). Methods The genome-wide association study (GWAS) data on relative sugar intake and breast cancer were analyzed by MR, and single nucleotide polymorphisms (SNPs) strongly associated with relative sugar intake were used as instrumental variables (IV), and five analysis methods were used to evaluate the causal relationship between relative sugar intake and breast cancer, including the MR-Egger, the weighted median, the inverse-variance weighted (IVW), the simple model and weighted model were used to assess the causal association between relative sugar intake and breast cancer using five analytical methods. Cochran's Q test was used to detect heterogeneity, MR-Egger regression for multivariate analysis, and “leave-one-out” method for sensitivity analysis. Results A total of nine SNPs strongly associated with relative sugar intake were screened as IV, and five methods, including IVW, MR-Egger, and simple model, showed that there was no causality between relative sugar intake and breast cancer (P>0.05); Cochran's Q test showed that there existed a certain degree of heterogeneity (P<0.10); and the multivariate analysis of MR-Egger regression revealed that The MR-Egger regression analysis showed that there was no pleiotropy (P>0.05); the sensitivity analysis of the “leave-one-out” method showed that the results of the MR analysis were stable. Conclusion There is no causal relationship between relative sugar intake and breast cancer, and relative sugar intake is not a risk factor for breast cancer.

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A Preliminary Study on the Predictive Effect of PAX1 and JAM3 Methylation in Cervical Exfoliated Cells for Cervical Intraepithelial Neoplasia

ZHOU Min, ZUO Juan, ZHOU Dai, CHEN Dan, HOU Da

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 123-128.

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Objective To evaluate the predictive power of paired box1(PAX1) and junctional adhesion molecule 3(JAM3) gene methylation as combined biomarkers for high-grade cervical intraepithelial neoplasia (CIN). Methods From July 2023 to December 2024, patients who received HPV typing testing, liquid-based cytology (TCT) testing, PAX1 and JAM3 gene methylation testing, and colposcopy pathology at Changsha Maternal and Child Health Hospital were included in this study (n=144). Methylation-specific PCR was used to quantitatively detect the methylation levels of PAX1 and JAM3 genes in cervical cells, and the combined prediction and discriminant analysis of cervical lesions were carried out by combining dichotomous variables such as HPV typing, PAX1 gene methylation and JAM3 gene methylation. Results Among the 16 pathologically confirmed CIN2 patients, the positive rates of PAX1 and JAM3 gene methylation (68.8% and 56.3%, respectively) were significantly higher than those in the CIN1 group (6.4%, 6.4%) and chronic cervicitis group (1.2%, 1.2%) (P<0.001). Among patients with high-risk HPV infection, the risk ratio for progression to CIN2 was 6.32(95% CI: 3.45-11.28) in those with positive methylation. The combined detection model showed that when HPV16/18 was positive and any gene methylation was positive, the positive predictive value of CIN2 was 96.9% (95%CI: 82.4-94.3), and the F1 value was significantly better than that of single HPV detection (59.1%) (P<0.01). Conclusion PAX1/JAM3 dual gene methylation combined with HPV typing can significantly improve the early identification ability of high-grade cervical lesions (AUC=0.8789), provide a reliable basis for molecular typing for clinical decision-making, and is especially suitable for the shunt management of cytologically uncertain cases.

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The Effect of AMT Intervention on STAI Scores, Vascular Elasticity of Voluntary Whole Blood Donors and Analysis of the Best Beneficial Population

LI Tianshu, LUO Jia, GUAN Yao

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 129-133.

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Objective Study the effect of applied muscle tension (AMT) intervention on the State Trait Anxiety Inventory (STAI) score, vascular elasticity, and optimal benefit population of unpaid whole blood donors. Methods Prospectively select 600 blood donors who participated in blood donation from August 2023 to September 2024 as research objects, and randomly divide them into research group and control group according to the central random system, with 300 cases in each group. The control group received regular care for blood donation patients to complete blood donation, while the research group received AMT intervention to complete blood donation on the basis of the control group. The research group continued training for 2 months. Analyze and compare the changes in blood pressure and pulse rate, STAI score, vascular condition, adverse reactions, and influencing factors of two groups of blood donors before and after blood collection using logistic regression analysis. Results The research group showed a significant difference in systolic blood pressure difference and pulse rate difference compared to the control group, while there was no significant difference in diastolic blood pressure difference between the two groups of donors. Before blood collection, there was no significant difference in STAI-S and STAI-T between the two groups of blood donors; After blood collection, the STAI-S and STAI-T scores of the two groups of blood donors were significantly reduced, and the STAI-S and STAI-T scores of the study group were lower than those of the control group; Before blood collection, there was no significant difference in blood vessel conditions such as vessel diameter, blood flow velocity, vessel thickness, and vessel elasticity between the two groups of blood donors; After blood collection, the vascular diameter of the study group was thicker than that of the control group, and the blood flow velocity was slower than that of the control group. The number of people with thick and clear blood vessels and good vascular elasticity was significantly higher than that of the control group, and the values were better than before blood collectio. The total incidence of adverse reactions among blood donors in the control group higher than in the study group. First-time blood donation, female, age range (18-30 years old), weight<55 kg, and occupation as a school student are the main influencing factors for adverse blood donation reactions. Conclusion Interventions in muscle contraction and relaxation exercises can improve STAI scores, increase vascular elasticity. Primary blood donors, female, age range (18-30 years old), weight<55 kg, and occupation as school students are the main influencing factors for adverse reactions in blood donation. Therefore, more attention should be paid to this type of blood donor population. AMT can effectively reduce the incidence of adverse reactions, with the best beneficiaries being young women who donate blood for the first time.

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Oxycodone Activates AMPK/SIRT1 Signaling Pathway to Alleviate Cognitive Function Decline after Surgery in Elderly Fracture Model of SD Rats

SUN Hai, HUANG Teng, MAO Dongyu, LI Rong hua, REN Xiaoping, ZHU Ping

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 134-139.

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Objective To explore whether oxycodone improves postoperative cognitive decline in elderly fracture models by affecting the AMPK/SIRT1 signaling pathway. Methods SD rats were randomly divided into sham operation group, model group, and 10, 20, and 30 μg·kg-¹·d-¹ oxycodone dose groups, with 10 rats in each group Open tibial fracture surgery was used to construct a model of cognitive dysfunction after fracture surgery in the elderly After 2 weeks of dosing, cognitive function was assessed using the Morris water maze test. The pathological injury of hippocampus and the number of neurons in hippocampus were observed by HE staining and Nissl staining, and the level of interleukin (IL) -1β, IL-18, tumor necrosis factor-α (TNF-α) in hippocampus was detected by ELISA. The level of p-AMPK/AMPK, SIRT1, p-NF-κB/NF-κB protein in hippocampus was detected by Western blot. Results Compared with the sham operation group, the model group showed a notable decrease in cognitive function (P<0.05), and the hippocampal tissue presented pathological damage, and the number of neurons in hippocampus decreased, and the IL-1β, IL-18 and TNF-α in hippocampus up-regulated whereas the p-AMPK/AMPK and SIRT1 down-regulated (P<0.05). After the treatment of 20, 30 μg·kg-¹·d-¹ oxycodone, the above phenomena were significantly improved, and the high dose was better. Conclusion Oxycodone may inhibit the expression of hippocampal pro-inflammatory factors by activating the AMPK/SIRT1 signaling pathway, thereby improving the postoperative cognitive decline in elderly fracture models

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Clinical Observation of Dynamic Scalp Acupuncture Combined with Upper Limb Intelligent Feedback Training for Upper Limb Motor Dysfunction in Stroke Patients

FAN Lianbin, JIANG Dongsheng, CHE Xingwang, LI Hongrui, TANG Runhui, YUAN Huiping, LI Fei

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 140-144.

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Objective To research the clinical effects of dynamic scalp acupuncture combined with upper limbs intelligent feedback training for upper limb motor function in stroke patients. Methods Choose randomly 90 stroke patients as the research object from the patients of Fuyang People's Hospital, Anhui, from June, 2021 to December, 2022. All the patients were randomly divided into a matched group, scalp acupuncture group, and dynamic scalp acupuncture group, 30 cases in each group. All three groups received routine medication treatment. The patients in the matched group received the upper limb intelligent feedback training, the patients in scalp acupuncture group received the treatment of normal scalp acupuncture combined and then received routine scalp acupuncture, and the patients in dynamic scalp acupuncture group received the scalp acupuncture treatment combined with upper limb intelligent feedback training. The patients of three groups received treatment for 8 weeks. Then it is observed the treatment result of the upper limb function by using DASH, FMA-UE, WMFT and MBI, compared to prior treatment. Result After the treatment, the treatment scores of the three groups in FMA-UE, WMFT and MBI were higher than them of prior treatment (P<0.05), and the scores of the effort of scalp acupuncture group and dynamic scalp acupuncture group were much higher than the scores of the matched group in FMA-UE and WMFT, especially dynamic scalp acupuncture group. But the scores of scalp acupuncture group and dynamic scalp acupuncture group in DASH were obviously lower than that in the matched group. Conclusion Dynamic scalp acupuncture combined with upper limb intelligent feedback training can cure the stroke patients of upper limb motor dysfunction, and can improve upper limb motor function and activities of daily living.

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Study on Correlation between Post-Traumatic Stress Disorder and Coping Styles in Patients with Limb Fractures

GUO Ming, ZHANG Lili, ZHANG Li

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 145-149.

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Objective To analyze the status of post-traumatic stress disorder (PTSD) in patients with limb fractures and its correlation with coping styles. Methods Totally 560 patients with limb fractures admitted to Cangzhou People's Hospital from June 2021 to June 2024 were included in the survey. The general data questionnaire, post-traumatic stress disorder checklist-civilian (PCL-C) and simple coping style questionnaire (SCSQ) were used for investigation. The scores of dimensions of PCL-C and SCSQ were evaluated, and the correlation between the above scores was analyzed by Pearson correlation analysis model. Results Among patients with limb fractures, the positive rate of PTSD was 87.55% (485/554). The scores of re-experience dimension, high alertness dimension and emotional numbness dimension and total score of PCL-C in patients with limb fractures were (15.10±1.31) points, (15.95±1.24) points, (20.09±1.13) points and (51.14±2.20) points, and the scores of positive coping dimension and negative coping dimension and total score of SCSQ were (9.84±0.39) points, (10.80±0.52) points and (20.64±0.66) points. Pearson correlation analysis indicated that the scores of dimensions of PCL-C were negatively correlated with the score of positive coping dimension of SCSQ (P<0.05), and were positively correlated with negative coping dimension score of SCSQ (P<0.05). Conclusion Patients with limb fractures have a higher risk of PTSD, and the occurrence of PTSD is closely related to the coping styles.

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Risk Factor Analysis and Development of a Predictive Model for Endometrial Malignancies in Postmenopausal Bleeding

LUO Lili, HUANG Yao, LIU Xiaohua, ZHOU Dai, Liu Jun

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 150-154.

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Objective By collecting clinical data such as postmenopausal bleeding (PMB) patients, risk factors, and endometrial ultrasound imaging characteristics, a model that predicts the risk of endometrial malignant lesions in women with PMB was constructed. Methods A total of 358 PMB patients treated at Liuyang People's Hospital from December 2019 to December 2024 were enrolled. Based on pathological results, patients with endometrial cancer (EC) and atypical hyperplasia (AH) were assigned to the case group, while others formed the control group. Clinical data on endometrial ultrasound imaging features and risk factors were collected, and a logistic regression analysis was performed retrospectively. Results (1) Statistically significant differences were observed between groups in endometrial thickness, echo uniformity, and blood flow grading (grade 0, grade I, grade II, grade III); (2) Significant differences were found in high-risk factors for endometrial malignancy, including age, menopause age, endometrial thickness, recurrent vaginal bleeding, obesity, diabetes, hypertension, and CA125 levels; (3) Logistic regression identified recurrent vaginal bleeding, obesity, diabetes, endometrial thickening, non-uniform echogenicity, and CA125>21U/mL as independent risk factors; (4) The “FBDNTC” prediction model (Frequency of bleeding, Body mass index, Diabetes, Non-uniform echogenicity & Thickness, CA125) was established, with scores ranging from 0 to 21. At a cutoff of ≥8 points (maximizing Youden index), sensitivity was 84.7%, specificity 74.6%, and AUC 0.881(95%CI: 0.845-0.917). Conclusion The FBDNTC risk prediction model integrates clinical and ultrasound features to effectively assist clinicians in stratifying endometrial malignancy risk, optimizing diagnosis and treatment.

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Diagnostic Study of Spiral CT and Three-Dimensional Reconstruction Technique in Evans Typing and Stability of Intertrochanteric Fracture of Femur

SHI Pei, CHENG Houqing, LE Jianping, KUANG Haishan, CHENG Guangjin

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 155-161.

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Objective To explore the diagnostic value of spiral CT and three-dimensional reconstruction technique in Evans typing and stability of intertrochanteric fracture of femur (IFF). Methods A retrospective analysis was conducted on the case data of 133 IFF patients treated in our hospital from January 2021 to February 2025. All patients underwent X-ray examination and spiral CT examination. The diagnostic value of the two examination methods for Evans classification and stability was compared. Results Intraoperative diagnosis of Evans typing in all patients showed that there were 47 cases of type I, 8 cases of type II, 35 cases of type III, 20 cases of type IV and 23 cases of type V. Compared with intraoperative diagnosis, X-ray diagnosis had certain diagnostic efficiency on Evans typing of IFF patients. Spiral CT combined with three-dimensional reconstruction technique had high diagnostic efficiency on Evans typing, and the accuracy rates of different types were 94.74%, 90.98%, 81.95%, 81.20% and 90.23% respectively. However, for type II and type IV patients, the consistency with intraoperative diagnosis was the lowest among the 5 types (Kappa=0.353, 0.379). The accuracy of the two diagnostic methods was significantly different. The diagnostic accuracy rates for external wall integrity, coronal fracture and fracture end stability were higher than those of X-ray (P<0.05). Conclusion IFF patients with different Evans types have different CT signs. Spiral CT and three-dimensional reconstruction technique have high diagnostic value on Evans typing and stability of IFF patients.

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Survey on Service Needs of Caregivers of Children with Febrile Seizures and Analysis of Influencing Factors

LU Anli, WANG Xuejing

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 162-168.

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Objective To investigate the service needs of caregivers of children with febrile seizures (FS) and analyze the influencing factors, so as to provide a basis for formulating targeted intervention strategies. Methods A convenience sampling method was used to select 218 caregivers of FS children admitted to the Chuzhou First People's Hospital from January to December 2024 as the study subjects. A self-designed Caregiver Service Needs Questionnaire for FS Children, the Generalized Anxiety Disorder-7(GAD-7), the Patient Health Questionnaire-9(PHQ-9), and a general information questionnaire were used for investigation. Multivariate logistic regression analysis was employed to analyze the influencing factors of caregivers' service needs. Results The total score of caregivers' service needs was (55.11±12.14). The scores of each dimension, from highest to lowest, were: health information and education (11.41±4.27), home care and follow-up management (9.94±3.92), follow-up and communication channels (9.51±3.40), psychological support and emotional counseling (8.72±3.38), first aid and skills training (8.55±4.12), and social/economic support (6.99±2.28). Multivariate analysis showed that the child's first seizure episode, hospitalization, complex FS, highest fever temperature≥39℃, female caregiver, and lower education level were independent influencing factors for higher service needs (P<0.05).87.61% of caregivers obtained health information through the internet/social media, while only 29.82% had received formal health education before admission. Conclusion Caregivers of FS children have the highest demand for health information and skills training. The severity of the child's condition and the caregiver's gender and education level are the main influencing factors. Medical institutions should provide structured health education for specific groups and strengthen the supply of authoritative online information.

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Effect of Scraping and Auricular Point Pressing Therapy Combined with Celecoxib on Pain Scores in Patients with Qi-Stagnation and Blood-Stasis Type Cervical-Shoulder Syndrome

ZHENG Jun, LIU Hongfei, LIANG Xiaoming

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 169-175.

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Objective To evaluate the effect and clinical significance of scraping and auricular point pressing therapy combined with celecoxib on pain scores in patients with cervical-shoulder syndrome of the qi-stagnation and blood-stasis type. Methods From January 2022 to December 2024, 219 patients were enrolled and randomly assigned to three groups: a Western medicine group treated with celecoxib, a traditional Chinese medicine group treated with scraping and auricular point pressing, and a combination group treated with both approaches (n=73 each). The treatment course lasted 4 weeks. Cervical pain was assessed using the visual analogue scale (VAS) and neck disability index (NDI). Levels of pain mediators [β-endorphin (β-EP) and cyclooxygenase-2 (COX-2)], cervical range of motion (ROM), traditional Chinese medicine (TCM) syndrome scores, Barthel index, clinical efficacy, and safety were also compared. Results The overall analysis showed no statistically significant differences in VAS and NDI scores among the three groups before treatment. As the treatment course progressed, the VAS and NDI scores in each group showed a gradual downward trend, with scores at 2 weeks and 4 weeks of treatment being lower than before treatment. Moreover, the scores in the combination group were lower than those in the Western medicine group and the traditional Chinese medicine group at all time points. After 4 weeks of treatment, compared with the Western medicine group and the traditional Chinese medicine group, the combination group showed an increase in β-EP levels, a decrease in COX-2 levels, significant improvement in ROM in all directions, lower traditional Chinese medicine syndrome scores, and higher Barthel Index scores, with all intergroup differences being statistically significant. The overall clinical effective rate of the combined group was significantly higher than that of the Western medicine group and the traditional Chinese medicine group. No serious adverse events were observed; only mild subcutaneous bruising occurred in a few patients. Conclusion Scraping and auricular point pressing therapy combined with celecoxib provides progressive relief of cervical-shoulder pain in patients with qi-stagnation and blood-stasis type syndrome. The therapy improves cervical mobility, enhances quality of life, and demonstrates good safety, supporting its clinical application.

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Analysis of the Value of Transvaginal Color Doppler Ultrasound Combined with Hb, NLR, TIMP-1 and sFlt-1 in the Differential Diagnosis of Uterine Fibroids and Adenomyosis

LUO Junlian, HUANG Zhihao, Rao Wenting

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 176-180.

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Objective To investigate the value of transvaginal color Doppler ultrasound (TVCDS) combined with peripheral blood hemoglobin (Hb), neutrophil-to-lymphocyte ratio (NLR), serum tissue inhibitory factor of metalloproteinase 1 (TIMP-1), and soluble fms-like tyrosine kinase-1 (sFlt-1) in differential diagnosis of uterine fibroids (UF) and adenomyosis (Am). Methods 143 cases of patients with pathologically confirmed UF and Am admitted to the First People's Hospital of Zigong from June 2023 to May 2024 were selected. They were divided into the UF group (81 cases) and Am group (62 cases). The clinical data, including peripheral blood Hb, NLR, serum TIMP-1 and sFlt-1 were compared between the two groups. The risk factors of Am, the value of levels of peripheral blood Hb, NLR, serum TIMP-1 and sFlt-1 for the differential diagnosis of UF and Am, and the diagnostic value of TVCDS combined with these four indicators in distinguishing UF from AMS were analyzed. Results The proportion of dysmenorrhea, prolonged menstruation, levels of peripheral blood NLR, serum TIMP-1 and sFlt-1 were higher than the UF group, level of peripheral blood Hb was lower than the UF group. Multivariate Logistic regression analysis showed that dysmenorrhea, prolonged menstrual period, peripheral blood Hb, NLR, serum TIMP-1 and sFlt-1 were independent risk factors for Am. ROC analysis showed that the area under the curve (AUC) values for differential diagnosis of UF and Am at levels of peripheral blood Hb, NLR, serum TIMP-1, and sFlt-1 were 0.743, 0.842, 0.746, and 0.850. The sensitivity and accuracy of combined detection of TVCDS, peripheral blood Hb, NLR, serum TIMP-1 and sFlt-1 in the differential diagnosis of UF and Am were higher than those of single detection of each index; the sensitivity of TVCDS alone was higher than that of serum TIMP-1 and sFlt-1, and the accuracy was higher than that of peripheral blood Hb, NLR and serum TIMP-1; the specificity of sFlt-1 alone was higher than that of peripheral blood Hb, NLR and serum TIMP-1, and the accuracy was higher than that of serum TIMP-1. Conclusion TVCDS combined with peripheral blood Hb, NLR, serum TIMP-1, sFlt-1 was more advantageous for the differential diagnosis of UF and Am.

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The Value of SWE Combined with R-VQS Technique for Multidimensional Quantitative Assessment in the DIAGNOSIS of Hashimoto's Thyroiditis

QIU Zhiling, LI Jie, SHI Yingping, LI Yiqiong, LAN Sirong

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 181-186.

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Objective To explore the application value of shear wave elastography (SWE) combined with holographic vascular hardness analysis (R-VQS) in the diagnosis of Hashimoto's thyroiditis. Methods A total of 70 female patients with new-onset HT or previous HT who had not received antithyroid medication who presented to our hospital from July 2022 to July 2023 were selected. The healthy control group consisted of 30 age-matched healthy females who underwent physical examination at our hospital during the same period of time. The 70 patients with HT were divided into 56 cases in the abnormal thyroid function HT group and 14 cases in the normal thyroid function HT group according to normal thyroid function or not. Total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), thyroglobulin antibody (TgAb), carotid intima-media thickness (IMT), carotid PWV, stiffness index (SI), right common carotid artery systolic diameter (SD), vascular wall displacement (VWD), and Young's modulus were compared among the three groups, and the diagnosis of HT was made by ROC. To analyze the value of Young's modulus values for diagnosis of HT patients. Results Comparing the levels of TC, TG and TgAb in the three groups, the healthy control group<normal HT group<abnormal HT group; comparing the levels of HDL-C and LDL-C in the three groups, the healthy control group>normal HT group>abnormal HT group. Comparison of IMT and VWD levels among the three groups showed no statistically significant difference; comparison of SD, SI and PWV levels among the three groups showed that the healthy control group>the metabolic function normal HT group<the metabolic function abnormal HT group. Comparing the values of Young's modulus among the three groups, healthy control group<normal HT group<abnormal HT group. After ROC curve processing, the results show that the area under the curve of Young's modulus value in diagnosing HT patients is 0.818±0.039 (P<0.001, 95% CI: 0.741~0.895), the best cutoff value is 56.980 KPa, sensitivity is 0.711, and specificity is 0.829. Conclusion HT patients are often accompanied by dyslipidemia. Using SWE combined with R-VQS technology can diagnose HT patients, and help to find carotid artery lesions early, which has certain reference significance for the treatment and prognosis of HT patients.

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Influences of Along the Hand Yangming Meridian Copper Moxibustion Combined with Exercise Therapy on Rehabilitation Effect in Patients with Upper Limb Hemiplegia after Stroke

ZHENG Yulin, KANG Qun, GU Yujie, Wu Yu

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 187-191.

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Objective To explore the influences of along the hand yangming meridian copper moxibustion combined with exercise therapy on rehabilitation effect of upper limb hemiplegia after stroke. Methods According to random number table method, 68 patients with upper limb hemiplegia after stroke were divided into control group (routine drugs treatment and exercise therapy, 4 weeks) and observation group (along the hand yangming meridian copper moxibustion on basis of control group, 4 weeks), 34 cases in each group. The clinical curative effect, scores of traditional Chinese medcine (TCM) syndromes, upper limb motor function, activities of daily living, surface electromyographic signals and range of motion in the two groups were compared. Results The total response rate of treatment was significantly higher in observation group than control group [88.24% (30/34) vs 67.65% (23/34), P<0.05]. After treatment, scores of TCM syndromes (upper limb hemiplegia and weakness, limb numbness, dysphasia), integrated electromyogram (IEMG) of biceps under elbow extension state and co-contraction ratio (CR) of biceps/triceps were decreased, while scores of Fugl-Meyer Assessment-Upper Extremity (FMA-UE) and Barthel index (BI), IEMG of affected biceps under elbow flexion state, IEMG of biceps under elbow flexion/extension states and range of motion (shoulder, elbow, wrist and hand joints) were increased in both groups, and the above indexes were better in observation group than control group (P<0.05). Conclusion The along the hand yangming meridian copper moxibustion combined with exercise therapy can effectively improve upper limb motor function, regulate co-contraction pattern of muscles and improve activities of daily living in patients with upper limb hemiplegia after stroke.

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Application of 3D-Printed Patient-Specific Guides Combined with Limited Decompression in Lumbar Interbody Fusion for Degenerative Scoliosis

MA Shuang, FENG Ke, DU Kairan, ZHANG Xinbo, WANG Yaosheng

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 192-196.

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Objective To evaluate the clinical value of three-dimensional (3D) reconstructive guide-assisted accurate pedicle screw placement combined with spinous process-preserving modified posterior lumbar interbody fusion (mPLIF) in degenerative spinal deformity. Methods Eighty patients were prospectively randomized into a guide group (n=40, 3D guide-assisted screw placement + mPLIF) and a conventional group (n=40, fluoroscopy-guided screw placement + open PLIF). Perioperative parameters (operation time, radiation exposure, blood loss), screw placement accuracy (Lu classification), and 1-year outcomes (VAS, ODI, JOA) were compared. Results The guide group showed significantly shorter operation time, fewer radiation exposures, reduced screw placement duration, and lower blood loss. The guide group achieved higher acceptable screw rates and precision . At 1-year follow-up, the guide group demonstrated better pain relief, functional recovery, and neurological improvement. In terms of complications, the overall incidence rate of the guide plate group was significantly lower than that of the conventional group. Conclusion Compared with the traditional open PLIF surgery under fluoroscopy, the 3D printed individualized guide plate combined with limited decompression surgery plan significantly optimizes the surgical process, which is reflected in the significant reduction of operation time, intraoperative blood loss, and radiation exposure for both the doctor and the patient. Moreover, it improves the accuracy and safety of screw placement, thereby reducing the risk of complications such as intraoperative nerve injury and postoperative infection.

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A Study About the Impact of Different Tools on the Accommodative Microfluctuations in Myopic Children During Near Work

CHEN Jiongpu, ZHONG Dingjuan, XIANG Aiqun, CHEN Jiao, LUO Dongqiang, GUO Yingzhuo, HE Shuxi, WANG Hua

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 197-202.

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Objective To explore the effects of different tools on accommodative microfluctuations (AMFs) in myopic children during near work. Methods A randomized controlled trial was conducted. A total of 102 myopic children aged 7 to 12 (42 males and 60 females) who visited the Optometry Center of Hunan Provincial People's Hospital from February 2023 to May 2024 were recruited. They were divided into the 20 minutes group (46 cases) and the 40 minutes group (56 cases) according to different eye-using time, And divided into the book group (35 cases), the tablet group (33 cases), and the mobile phone group (34 cases) according to different tools. Participants first underwent general examinations, including vision tests, slit-lamp examinations, and axial length measurements. Subsequently, while engaging in near-vision tasks, participants had their accommodative microfluctuations high-frequency component values (HFC) and pupil diameter measured for the first time using Acomoref2. Then, they were required to read some words on a book, tablet or mobile phone at a near distance under normal lighting conditions. Then, the HFC and pupil diameter were immediately measured for the second time. One way repeated measures analysis of variance (ANOVA) was used for data analysis. Results After 40 minutes of near work, the changes in AMFs caused by reading on mobile phones, tablets, and books were the same, and there was no significant statistical difference among the three groups. After 20 minutes of near work, there was no statistical significance in the HFC when looking at the targets at infinity, 50 cm, and 33 cm among the three groups. However, there was a significant difference in the change value of HFC when looking at the target at 1 m. There was a significant difference in the change of HFC when looking at the target at infinity between 20 minutes and 40 minutes near work. Conclusion For myopic children aged 7-12, reading books at a close distance within 20 minutes makes the accommodative microfluctuations more tense compared to using tablets or mobile phones. As the near work time increases from 20 minutes to 40 minutes, the accommodative microfluctuations of myopic children aged 7-12 gradually changes from unstable to stable.

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Incidence and Risk Factors of Sleep Disorders During Radiotherapy for Lung Cancer

ZHANG Chao, WANG Fengjuan, DU Wen, LIU Qi, LI Xia, LIU Fengjuan

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 203-207.

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Objective To investigate the incidence and risk factors of sleep disorders during radiotherapy for lung cancer and propose preventive suggestions. Methods A total of 206 patients who underwent radiotherapy for lung cancer from January to April 2025 were enrolled in the study. The Pittsburgh Sleep Quality Index (PSQI) was used to assess the incidence of sleep disorders during radiotherapy. The Visual Analog Scale (VAS) was used to evaluate pain intensity. The Self-Rating Anxiety Scale (SAS) and Self-Rating Depression Scale (SDS) were used to evaluate psychological states. Patients were divided into the sleep disorder group and the non-sleep disorder group. Clinical data were compared between groups, and a logistic regression model was used to identify risk factors for sleep disorders during radiotherapy. Results The incidence of sleep disorders was 24.27% (50 cases) before radiotherapy, 33.98% (70 cases) after 2 week of radiotherapy, 35.92% (74 cases) after 4 week of radiotherapy, and 37.86% (78 cases) after 6 weeks of radiotherapy. The proportions of patients ≥60 years old, with diabetes, anxiety, depression, concurrent chemotherapy, and cancer-related fatigue in the sleep disorder group were significantly higher than those in the non-sleep disorder group (P<0.05). Age and pain VAS score of the sleep disorder group were significantly higher than those of the non-sleep disorder group (P<0.05). Logistic regression analysis showed that age ≥60 years, diabetes, anxiety, depression, pain VAS score, concurrent chemotherapy, and cancer-related fatigue were independent risk factors for sleep disorders during radiotherapy for lung cancer (P<0.05). Conclusion Lung cancer patients have a relatively high risk of sleep disorders and radiotherapy increases the incidence of sleep disorders. Advanced age, diabetes, anxiety, depression, VAS score of pain, concurrent chemotherapy, and cancer-related fatigue are main risk factors for sleep disorders. Targeted interventions addressing these factors are recommended for clinical practice.

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Influence of 1% Povidone Iodine Combined with SRP on Supragingival Plaque, Gingival Inflammation and Patient Acceptance in Patients with Stage I-II, Grade A-B Periodontitis

WANG Shu, GUO Gang, CUI Shanjun

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 208-212.

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Objective To investigate the influence of 1% povidone iodine combined with subgingival scaling and root planing (SRP) on supragingival plaque, gingival inflammation and patient acceptance in patients with stage I-II, grade A-B periodontitis. Methods 180 patients with stage I-II, grade A-B periodontitis who were treated in the hospital from December 2021 to December 2024 were selected and randomly divided into combined group (n=90, 1% povidone iodine combined with SRP) and SRP group (n=90, SRP) by envelope method. Plaque control effect[plaque index (PLI), plaque proportion], gingival inflammation degree [gingival index (GI), gingival blood flow, bleeding on probing (BOP)], periodontal tissue damage indexes [periodontal pocket depth (PD), total number of residual dental stone sites on root surface, clinical attachment loss (CAL)], Chinese version of acceptance of illness scale (AIS-CHI) score and safety were compared between the two groups. Results At 3 months after treatment, the PLI and proportion of plaque in the two groups were decreased, and the indexes in combined group were lower than those in SRP group. The GI, BOP and gingival blood flow were reduced in the two groups, and the indexes in combined group were lower. The PD, CAL and total number of residual dental stone sites on root surface in both groups were declined, and the indexes were lower in combined group than those in SRP group (P<0.05). The AIS-CHI score in the two groups was increased, and the score in combined group was higher compared to SRP group. There was no statistical difference in the total incidence rate of adverse reactions between the two groups. Conclusion 1% povidone iodine combined with SRP in the treatment of stage I-II, grade A-B periodontitis can control plaque, relieve gingival inflammation, improve the degree of periodontal tissue destruction, and enhance the acceptance of patients, with controllable safety.

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Infectious Occupational Exposure Analysis of Medical Staff in a Tertiary First-classStomatological Hospital

TANG Na, HE Si, YAO Jun, ZHU Dingyu, LI Sha, ZHOU Junhua

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 213-217.

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Objective To analyze the current situation and causes of infectious occupational exposure of medical staff in stomatological hospitals. Methods A tertiary first-class stomatological hospital was selected as the study site, and the occupational exposure data reported by all medical staff in the hospital from 2019 to 2023 were collected, and compared and analyzed from three aspects: basic situation, exposure situation, emergency treatment after exposure and reporting situation. Results (1) From 2019 to 2023, 84 medical staff had occupational exposure, and the occupational exposure rate was 2.23%; The highest occupational exposure rate was medical workers under 30 years old (3.32%) and students (4.15%), followed by doctors (1.79%). (2) There were statistically significant differences in occupational exposure composition among all groups (P<0.05). Among them, the main links of occupational exposure occurred in the final disposal of diagnostic instruments (50.00%), index finger (59.52%), hollow needle puncture wound (57.14%), blood exposure (89.29%) and oral and maxillofacial surgery (38.10%). (3) 80.95% of the exposed workers could report to the hospital infection-control department in time after occupational exposure, 51.19% of the exposed workers washed the exposed area for less than 5 minutes after occupational exposure, and 30.95% of them were less than 3 minutes (P<0.05).85.71% of medical workers were disinfected after exposure, and 47.62% were not treated properly after exposure. Conclusions Medical staff in specialized dental hospitals face a relatively high risk of infectious occupational exposure. Those under the age of 30, including students, constitute a key demographic of occupational exposure incidents. Critical moments for such exposure include the final handling and disposal of contaminated instruments, finger injuries, and hollow needle punctures. The Department of Oral and Maxillofacial Surgery is identified as a high-risk area for occupational exposure. It is imperative to enforce stringent standard precautionary measures among medical personnel, enhance their awareness of occupational protection, and improve their operational skills to prevent infections following occupational exposure in specialized dental hospitals.

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Determination of Residual Solvents in Tolbutamide Hydrochloride by GC

YANG Changhua, HU Wengsheng, LIU Xiangguang

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 218-222.

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Objective To establish a gas chromatography method for the determination of residual solvent of topazone hydrochloride. Methods The analysis was conducted using a gas chromatograph with an Agilent 7697A headspace sampler and a DB-624 capillary column (30 m×3.2 mm, 1.8 µm). Nitrogen was used as the carrier gas, and hydrogen and air served as fuel gases. The split ratio was set at 20∶1 with a flow rate of 2 ml/min. The temperatures for the FID detector and inlet were maintained at 250℃ and 200℃, respectively. Regarding headspace conditions, the equilibrium temperature was 90℃, the loop temperature was 100℃, and the transfer line temperature was 110℃, with an equilibrium time of 30 min and a cycle time of 35 min. The oven temperature program began at 40℃ for 8 min, then increased to 155℃ at a rate of 10℃/min and was held for 4 min. Results The recovery rates for the five target solvents ranged from 90% to 110%, with RSD ≤ 3.0%. The resolutions between ethanol/acetone, acetone/isopropanol, isopropanol/isopropyl ether, and isopropyl ether/toluene were 6.5, 2.3, 14.0, and 60.3, respectively. The linear correlation coefficients (r) for all five substances were ≥0.9950 within the tested concentration range. Conclusion The method is simple, accurate and repeatable to detect the residual solvent of topazone hydrochloride.

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Application of a Joint Medical-Nursing Assessment in the Construction of a Prediction Model for Port-Related Infections

ZHANG Yang, GAO Jie, LIU Weiwei, WU Qiong, LI Hongchao, DONG Qian

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 223-228.

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Objective To explore the application effect of joint medical-nursing assessment in the construction of a prediction model for port-related infections. Methods A total of 550 patients with port applications admitted to a tertiary hospital from July 2023 to December 2024 were selected as the subjects of this study, among whom 50 patients who developed infections were designated as the infection group, and 500 patients without infections were designated as the non-infection group. The reasons for infections in patients with port applications were analyzed through joint medical-nursing assessment. A prediction model was established using univariate analysis and logistic regression analysis, and a nomogram was used for visual expression. Results The main causes of infection in the infusion port were parenteral nutrition, gynecological system cancer, hematological system cancer, diabetes, and intubation time>110 days (P<0.05). The area under the ROC curve of the prediction model was 0.851, corresponding to a sensitivity of 0.802 and a specificity of 0.811. The average absolute error between the actual and predicted values of the prediction model was 0.011, indicating a high degree of agreement between the calibration curve and the ideal curve; the Hosmer-Lemeshow goodness-of-fit test result was χ²=2.873 (P=0.931). Conclusion The prediction model constructed through joint medical-nursing assessment has good predictive performance and can provide important reference for clinical staff to identify high-risk patients for port-related infections at an early stage.

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Effect of a Family Support Program Based on the Actor-Partner Interdependence Model on Postpartum Depression and Breastfeeding in Parents of Hospitalized Preterm Infants

ZHANG Liuyi, LIU Bin, GUO Yafen, SHEN Jinhua, ZHANG Xueqin

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 229-234.

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Objective To explore the effects of a family support program for hospitalized preterm infants based on Actor-Partner Interdependence Model on postnatal depression, breastfeeding, and Family Intimacy and Adaptability of preterm infants' parents. Methods A convenience sampling method was used to select 97 cases of preterm infants and their families who were hospitalized in the neonatal care unit of a tertiary general hospital in Hunan Province from March to September 2024, and divided into the experimental group and the control group according to the method of randomized numerical tables. The control group implemented routine nursing measures such as health education and early activity guidance; the experimental group implemented a family intervention program based on Actor-Partner Interdependence Model. Breastfeeding was collected at 1 month after discharge of preterm infants, and postnatal depression and Family Intimacy and Adaptability scores of preterm infants' parents were collected at 42 days postpartum. Results Five groups of families dropped out during the intervention, and ultimately 49 families with preterm infants in the experimental group and 43 families with preterm infants in the control group completed the study. Exclusive breastfeeding, mixed feeding, formula feeding were 39, 7, and 3 cases in the experimental group and 24, 15, and 4 cases in the control group, respectively, and the breastfeeding of preterm infants in the experimental group was better than that in the control group (Z=2.323, P<0.05); 38 cases of parents in experimental group and 22 cases of parents in control group were free of depression, and the detection rate of postnatal depression in preterm infants' parents was lower in the experimental group than in the control group (χ²=7.450, P<0.05); the intimacy score of families in the experimental groups was (56.50±8.59), higher than that of families in the control group (45.65±6.58) (t=6.827, P<0.05), and the adaptability score of families in the experimental groups was (46.80±10.43), higher than that of families in the control group (40.77±9.01) (t=2.974, P=0.004). Conclusion The family support program for hospitalized preterm infants based on Actor-Partner Interdependence Model can improve the breastfeeding success rate of preterm infants, reduce the probability of postnatal depression in preterm fathers and mothers, and improve family intimacy and adaptability, which can be used in clinical practice.

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Correlation Study between Otoplasty and Quality of Life in Aesthetic Patients

XIE Weicheng, LI Gaofeng

Journal of Hunan Normal University(Medical Science). 2025, 22(6): 235-240.

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Objective To investigate the impact of otoplasty on the quality of life (QoL) of aesthetic patients and analyze changes in their QoL before and after surgery. Methods Patients who underwent otoplasty at the Department of Plastic and Laser Aesthetics, Hunan Provincial People's Hospital, between January 2022 and June 2024 were selected based on inclusion and exclusion criteria. A self-designed questionnaire was used to collect demographic data, and Social Function and Psychological Function modules of FACE-Q (facial aesthetic evaluation questionnaire) scoring system were applied to assess QoL pre- and postoperatively. Data were analyzed using SPSS 30.0. Results Thirty-eight patients were enrolled, with 30 completing pre- and postoperative questionnaires (follow-up rate: 78.9%). The cohort included 16 males and 14 females, aged 16-47 years (mean: 24.9±7.6). The average preoperative social function score improved from 70.1±15.1 to 78.6±12.6 postoperatively (P<0.01), with a mean increase of 8.5 points. The psychological function score increased from 73.3±15.6 to 80.5±11.9 (P<0.01), with a mean increase of 7.2 points. There was no significant statistical difference in the improvement of the social and psychological functions of those who were of different ages, education, motivation for beauty seeking and marital status. Female patients showed significantly greater improvement in psychological function compared with male patients. Conclusion Ear plastic surgery can significantly improve the quality of life of those who seek beauty and improve their social and psychological function scores. This improvement is independent of factors such as age, education, motivation for seeking beauty, and marital status. The improvement of psychological function scores is related to gender.

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25 December 2025, Volume 22 Issue 6

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25 December 2025, Volume 22 Issue 6