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25 June 2025, Volume 22 Issue 3
    

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    Basic Medicine
  • A novel biguanide derivative N-1-hexyl-N-5- (4-trifluoromethoxy) chloroguanidine in combination with sorafenib inhibits the proliferation of bladder cancer cells and its regulatory mechanism
    ZHOU Xiaochen, HE Fanrong, WANG Weifan, XIE Yijun, XIAO Di, LI Duo, YANG Xiaoping
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 1-7.
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    Objective Bladder cancer presents a significantly clinical challenge due to its high recurrence rate and the limited therapeutic options. Although Sorafenib has shown the promising clinical efficacy in liver cancer, its long-term use is often associated with adverse effect. Additionally, Metformin has demonstrated the potential in cancer therapy; however, its use with high doses may lead to side effect. This study is based on an intermediate derivatization strategy to design and synthesize a novel biguanide derivative, namely 6C, with the aim to evaluate its effect, as a monotherapy and in combination with Sorafenib, on bladder cancer cell proliferation and investigate its underlying mechanisms. Methods Firstly, the impact of 6C and Sorafenib alone on bladder cancer cell proliferation was assessed using the MTT assay. Subsequently, the effect of these drugs, both individually and in combination, on bladder cancer cell proliferation were further evaluated by colony formation assay. Finally, Western blotting was employed to examine the expression of epidermal growth factor receptor (EGFR) and autophagy-related proteins LC3-II and Beclin1 in bladder cancer cells by the treatment with 6C and Sorafenib combination therapy. Results Both 6C and Sorafenib alone significantly inhibited the proliferation of bladder cancer cells. The combination of 6C and Sorafenib exhibited a marked synergistic effect, demonstrating more potent inhibition of cell proliferation compared to either drug used alone. Mechanistic investigations revealed that the combination treatment significantly decreased the expression level of EGFR and upregulated the levels of autophagy markers LC3-II and Beclin1, thereby inducing autophagy of these cells. Conclusion The combination of 6C and Sorafenib decreases EGFR expression and induces autophagy, which inhibits bladder cancer cell proliferation. This study suggests that the combination therapy using 6C and Sorafenib may offer a novel therapeutic strategy with the promising application for bladder cancer treatment.
  • Analysis of dacarbazine resistance in melanoma mediated by abcb1 overexpression and its reversal through targeted inhibition
    LIAO Yilong, DENG Yuanxiong
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 8-14.
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    Objective The aim of this study was to explore the resistance of dacarbazine in melanoma A375 cells driven by the high expression of ABCB1 gene and its targeted reversal strategy through the construction of melanoma drug-resistant cell lines. Methods A375 melanoma dacarbazine resistant cell line (A375-DacR) was constructed by gradually increasing Dacarbazine concentration and long-term induction of melanoma A375 cells. CCK-8 assay and flow cytometry were used to evaluate the drug resistance level of drug-resistant cell lines. The expression levels of ABCB1 gene and its coding protein P-gp in A375 cells and A375-DACR cells were detected by qPCR and Western Blot. The cell cycle distribution was detected by flow cytometry. Dacarbazine resistant cell lines were treated with ABCB1-targeted inhibitors to evaluate the effect of resistance reversal. Results The dacarbazine-resistant A375 melanoma cell line (A375-DacR) was successfully constructed with a resistance index greater than 5. The mRNA and protein expression levels of ABCB1 in A375-DacR cells were significantly upregulated, highlighting its pivotal role in the development of drug resistance. Compared to parental A375 cells, A375-DacR cells exhibited a notable G0/G1 phase arrest. Following targeted inhibition of ABCB1, the expression of its encoded protein P-gp was reduced, leading to a decreased survival rate and clonogenic capacity of A375-DacR cells upon dacarbazine treatment. Conclusion Following long-term induction, the A375-DacR cell population exhibited a transition into the quiescent phase (G0 phase) as an adaptive response to the selective pressure of dacarbazine treatment. Moreover, the high expression of the ABCB1 gene and its encoded protein P-gp was identified as a key driver of dacarbazine resistance in melanoma A375-resistant cells. Inhibition of ABCB1 effectively restored the sensitivity of A375-DacR cells to dacarbazine. This study suggests that targeting ABCB1 may serve as an effective strategy for overcoming dacarbazine resistance in melanoma therapy.
  • Esketamine regulation miR-146a expression to alleviate inflammation and lung injury in sepsis-induced acute lung injury
    YANG Wenyu, YU Feng, LI Moyong, ZHANG Jingjing, CHEN Anji
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 15-19.
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    Objective To investigate whether esketamine exerts a protective effect against sepsis-induced acute lung injury (ALI) by regulating miR-146a expression. Methods A sepsis-induced ALI mouse model was established using lipopolysaccharide (LPS, 10 mg/kg). The therapeutic efficacy of esketamine was assessed via two administration routes: intratracheal instillation and intraperitoneal injection. Pathological changes in lung tissue were evaluated by histopathological examination. The lung wet/dry weight ratio (W/D) was calculated to assess pulmonary edema. Levels of IL-6, TNF-α, and IL-1β in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA). The expression levels of miR-146a, IL-6, TNF-β, and IL-1β in lung tissues were quantified via qRT-PCR. Results Esketamine alleviated LPS-induced lung injury in septic mice and significantly suppressed the expression of IL-6, TNF-α, and IL-1β in BALF and lung tissues. Concurrently, esketamine upregulated miR-146a levels. Conclusion Esketamine mitigates sepsis-induced ALI by activating miR-146a expression and suppressing NF-κB pathway-mediated inflammatory responses. Intraperitoneal administration demonstrated superior efficacy compared to intratracheal instillation, highlighting its potential as a clinical therapeutic strategy.
  • A novel immune strategy and detection method for preparing monoclonal antibodies based on mRNA-LNP immunogen
    ZHOU Wenting, FU Shuxiang, TIAN Fangyan, LUO Yinghui, DENG Canping, ZHU Shuying, YANG Kaihuai
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 20-28.
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    Objective To prepare monoclonal antibody (McAb) against COVID-19 in mice by using mRNA-LNP, the receptor binding region of the S protein of COVID-19 (RBD), as the immunogen. Methods This study used mRNA LNP containing the RBD protein sequence as an immunogen, . Immunization of Balb/c mice with RBD mRNA LNP via intramuscular injection. The RBD antibody titer in the serum of the mice was detected by flow cytometry (FCM) and enzyme-linked immunosorbent assay (ELISA) ; After achieving the standard for serum titer detection, stable hybridoma cells secreting RBD monoclonal antibodies were constructed using hybridoma fusion cell technology, followed by ascites production and antibody purification; Use immunofluorescence (IF), flow cytometry (FCM), enzyme-linked immunosorbent assay (ELISA), Western blot and other methods to identify its biological activity and detect the specificity of the obtained antibodies. Result After re screening, two hybridoma cells capable of stably secreting RBD McAbs were obtained and named 2D7D11 and 9B6H5, respectively. After testing, the Ig subclasses of 2D7D11 and 9B6H5 are both IgG2b, with the light chain being the kappa chain; Through IF, FCM, ELISA, and Western blot analysis, it was confirmed that the 2D7D11 and 9B6H5 RBD McAbs exhibit high specificity towards RBD protein or S-RBD (e1) - PET28 recombinant protein expressed in RBD mRNA LNP transfected cells. Conclusion This study successfully prepared two anti RBD specific monoclonal antibodies, 2D7D11 and 9B6H5, This provides an experimental basis for the delivery, translation, and modified expression of mRNA LNP as a novel immunogen in animals, the autonomous generation of humoral immune responses to produce antibodies, and the development of related detection methods and functional exploration; This provides an effective novel immune strategy for antibody development projects that are hindered by the inability to prepare effective immunogens due to various reasons.
    • Clinical Medicine
  • Observation on the efficacy of Waff Exercise combined with pelvic floor electrical stimulation biofeedback in the treatment of postpartum stress urinary incontinence
    TAN Kuailing, LIU Jiaojiao, LI Cuiying, ZU Yue'e, HUANG Fang, ZHOU Dai, PENG Yue
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 29-32.
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    Objective This study was designed to evaluate the clinical efficacy of Waff exercise combined with electrical stimulation and biofeedback in the treatment of postpartum stress urinary incontinence. Methods 90 patients with postpartum stress urinary incontinence treated in the Pelvic Floor and Postpartum Rehabilitation Center of Changsha Maternity and Child Health Hospital from February 2022 to March 2023 were selected . They were randomly classified into the control group and the intervention group, with 45 cases in each group. The control group was treated with conventional electrical stimulation and biofeedback for the pelvic floor, whereas the intervention group was treated with Waff exercise on the basis of the control group. The patients were supervised to complete all the treatments within 3 months. The results of the urine pad test and clinical symptoms, the questionnaire of Urinary Incontinence Short Form (ICIQ-UI) and the Urinary Incontinence Quality of Life Scale (I-QOL) were compared before and after the treatment. Results After treatment, the urine leakage in the one-hour urine pad test and the questionnaire of Urinary Incontinence Short Form (ICIQ-UI) in the two groups were decreased, while the patients' self-conscious symptoms, pelvic floor muscle strength and the Urinary Incontinence Quality of Life Scale (I-QOL) were improved. Compared with the control group, the patients in intervention group had statistically significant differences in subjective aspects, including self-conscious symptoms and quality of life, and objective aspects, e. g. , 1 hour urine pad test, the pelvic-abdominal dynamic disorder of postpartum women, and floor muscle strength. Conclusion Waff exercise combined the elctrical stimulation enhance the pelvic floor muscle's movement perception and pelvic floor muscles strength, and it reduces urine leakage to improve the life quality of patients.
  • Distribution of lower respiratory tract infection pathogens and the relationship between serum PCT, IL-6, CRP and disease severity, prognosis of patients with lung cancer and chronic obstructive pulmonary disease after chemotherapy
    WANG Menglan, FU Ran, LI Yue, WANG Yi, TANG Simin, WANG Ying, WAN Yufeng
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 33-36.
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    Objective To explore distribution of lower respiratory tract infection (LRTI) pathogens and the relationship between serum procalcitonin (PCT), interleukin-6 (IL-6), C-reactive protein (CRP) and disease severity, prognosis of patients with lung cancer and chronic obstructive pulmonary disease (COPD) after chemotherapy. Methods The clinical data were collected from 116 patients with lung cancer, COPD and LRTI after chemotherapy (infection group) between September 2022 and March 2024, while 50 patients with lung cancer, COPD and non-LRTI during the same period were enrolled as non-infection group. According to scores of acute physiology and chronic health evaluation II (APACHE II), patients in infection group were divided into mild-moderate group and severe group. The changes of PCT, IL-6 and CRP in different groups were observed. All patients in infection group were followed up for 3 months, and they were divided into death group and survival group according to prognosis. The predictive efficiency of serum PCT, IL-6 and CRP for prognosis of LRTI was evaluated by receiver operating characteristic (ROC) curves. Results The main pathogens of LRTI were Gram-negative bacteria (55.86%), Gram-positive bacteria (38.62%) and fungi (5.52%). The levels of PCT, IL-6 and CRP in infection group were higher than those in non-infection group (P<0.05). The levels of PCT, IL-6 and CRP in severe infection group were higher than those in mild-moderate infection group (P<0.05). The levels of serum PCT, IL-6 and CRP in death group were higher than those in survival group (P<0.05). Logistic regression analysis showed that PCT>5.133ng/mL, IL-6>52.630 pg/mL and CRP>87.949 mg/L were risk factors of poor prognosis (P<0.05). ROC curves analysis showed that sensitivity and specificity of serum PCT combined with IL-6 and CRP for evaluating prognosis of LRTI were higher (80.00%, 83.33%). Conclusion The distribution of LRTI pathogens is complex and diverse in patients with lung cancer and COPD after chemotherapy, and Gram-negative bacteria are the main pathogens. Serum PCT, IL-6 and CRP can be applied as important indexes to evaluate disease severity and prognosis.
  • Effects and clinical significance of amomum yam and crucian carp soup on the nutrition-inflammation-immune axis in patients with uremia of spleen-kidney yang deficiency type
    XIE Qinjiao, HE Zeyun, LI Dingwen, TANG Yu, ZHAO Hong
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 37-42.
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    Objective To investigate the effects and clinical relevance of Amomum yam and crucian carp soup, a traditional medicinal dietary formula, on the nutrition-inflammation-immune (NII) axis in patients with uremia characterized by spleen-kidney yang deficiency. Methods A total of 91 patients diagnosed with spleen-kidney yang deficiency and uremia with malnutrition were enrolled between July 2021 and July 2024. Using a random number table method, they were assigned to a control group (n=45) or an observation group (n=46). The control group received conventional hemodialysis combined with Jiawei Zhenwu Decoction, while the observation group was treated with Amomum-Yam-Carp Soup in addition to hemodialysis. Follow-up was conducted after three months. Clinical efficacy, Traditional Chinese Medicine syndrome scores, quality of life, inflammatory markers, and nutritional indices were assessed before and after treatment. The incidence of adverse reactions was recorded. Results The overall response rate in the observation group was significantly higher than in the control group (97.83% vs 84.44%). Compared with the control group, the observation group showed significantly lower scores for fatigue, cold extremities, shortness of breath, poor appetite, and lumbar/knee weakness after treatment. Levels of hemoglobin (Hb), albumin (ALb), transferrin (TRF), and blood urea nitrogen (BUN) were significantly increased in the observation group, while total cholesterol (TC) levels showed no significant change. Nutritional indicators, including mid-arm circumference (MAC), triceps skinfold thickness (TSF), and mid-arm muscle circumference (MAMC), were significantly improved. Immunological markers also indicated an increase in CD4+ and CD4+/CD8+ ratios and a decrease in CD8+ levels. Additionally, inflammatory markers such as interleukin-6(IL-6), high-sensitivity C-reactive protein (hs-CRP), and tumor necrosis factor-alpha (TNF-α) were significantly lower post-treatment. Quality of life scores were significantly higher in the observation group. The incidence of malnutrition after treatment was significantly lower in the observation group compared to the control group (8.70% vs 51.11%). Conclusion The administration of Amomum yam and crucian carp soup alongside hemodialysis significantly improved nutritional status, reduced systemic inflammation, and enhanced immune function in patients with spleen-kidney yang deficiency uremia, ultimately contributing to better quality of life.
  • Evaluation of the efficacy and safety of uterine massage combined with oxytocin in preventing postpartum hemorrhage in high-risk parturients
    MO Lihua, CHEN Dan
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 43-47.
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    Objective To evaluate the efficacy and safety of uterine massage combined with oxytocin in preventing postpartum hemorrhage (PPH) in high-risk parturients. Methods A prospective randomized controlled trial was conducted, enrolling 400 high-risk parturients who delivered in the obstetrics department of a tertiary hospital between January 2019 and December 2023. The participants were randomly divided into a control group and an observation group, with 200 cases in each group. The control group received routine oxytocin therapy, while the observation group received uterine massage in addition to oxytocin therapy. The primary outcomes measured included the duration of the third stage of labor, blood loss within 2 hours and 24 hours postpartum, and hemostasis time. Secondary outcomes included postpartum coagulation function, hemoglobin levels 1 hour postpartum, uterine involution, complication rates, hospital stay duration, and patient satisfaction. Results Compared with the control group, the observation group showed significant improvements in the duration of the third stage of labor (8.33±2.81 minutes vs 10.51±3.22 minutes, P<0.001), blood loss within 2 hours postpartum (187.45±46.52 mL vs 250.62±52.85 mL, P<0.001), blood loss within 24 hours postpartum (357.83±80.76 mL vs 455.23±98.42 mL, P<0.001), and hemostasis time (38.27±8.95 minutes vs 43.59±10.25 minutes, P<0.001). Additionally, the observation group experienced faster uterine involution, shorter hospital stays, and higher patient satisfaction. There was no significant difference in complication rates between the two groups. Conclusion Uterine massage combined with oxytocin is significantly more effective than oxytocin alone in preventing postpartum hemorrhage in high-risk parturients, with good safety. This combined treatment method has valuable potential for clinical application.
  • Clinical observation of trigger point blood-letting cupping therapy for post-stroke shoulder pain
    CHEN Yao, ZHANG Peiqin, LI Xinwu, HE Yongjia, SHE Chang
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 48-53.
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    Objective To evaluate the clinical efficacy of trigger point blood-letting cupping therapy in the treatment of post-stroke shoulder pain. Methods A total of 102 patients with post-stroke shoulder pain were enrolled and randomly divided into an observation group and a control group. The observation group received trigger point blood-letting cupping therapy on the basis of basic treatment and routine acupuncture, while the control group only received basic treatment and routine acupuncture. Both groups were treated for 2 weeks (10 times). The visual analogue scale (VAS) for pain, the Fugl-Meyer assessment (FMA) scale for upper limb function, the modified barthel index (MBI), the Hamilton anxiety scale (HAMA), the Pittsburgh sleep quality index (PSQI) were evaluated before treatment, after 1-week treatment, after treatment, and 1-month follow-up, and the frequency of non-steroidal anti-inflammatory drug (NSAIDs) use was recorded. Results The total effective rate was higher in the observation group than in the control group. Repeated measures ANOVA revealed that the time factor had a significant influence on all outcome measures: VAS, HAMA, and PSQI scores, as well as the frequency of non-steroidal analgesic use, decreased significantly over the course of treatment in both groups, while FMA and MBI scores increased significantly. Intergroup comparisons further demonstrated superior improvement in the observation group across multiple dimensions: For pain control (VAS) and sleep quality (PSQI), the observation group showed significantly lower scores from the end of treatment through the follow-up period. Regarding motor function (FMA) and activities of daily living (MBI), the observation group had significantly higher scores at the end of treatment and at follow-up. For anxiety (HAMA), no statistically significant difference was found between the groups during the treatment period, but the observation group's scores were significantly lower at follow-up. Concerning the frequency of analgesic use, it was already significantly lower in the observation group at one week of treatment, but no significant difference was observed between the groups post-treatment and at follow-up. Conclusion Acupuncture treatment can effectively alleviate pain, improve upper limb motor function and activities of daily living, and also has positive effects on reducing anxiety, improving sleep quality, and decreasing the reliance on non-steroidal analgesics in patients with post-stroke shoulder pain. The addition of trigger point bloodletting and cupping to conventional acupuncture therapy can further accelerate pain relief and early analgesic reduction, enhance the recovery of motor function and daily living abilities, and provide superior long-term stability in mood and sleep improvement. The overall therapeutic effect of the combined protocol is significantly superior to conventional acupuncture alone, demonstrating value for clinical application and promotion.
  • Comparison of stability between self-ligating brackets and conventional brackets in the treatment of mandibular arch orthodontics in adults
    GAO Mingying, LIU Shaochen, FAN Ruixin, ZHANG Chunhui, ZHANG Jie
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 54-58.
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    Objective To compare stability outcomes after mandibular arch orthodontic treatment for mild to moderate crowding malocclusions between a self-ligating system and a conventional system. Methods From March 2021 to March 2024, 40 patients who received orthodontic treatment in our hospital were randomly divided into two groups according to 1∶1 allocation ratio: self-ligating system (n=20) or conventional system (n=20). Orthodontic study models were collected at the debonding stage (T1), 1 month (T2), 3 months (T3), and 6 months (T4) after debonding, and the mandibular arch variables assessed were as follows: index of irregularity, intercanine width, first and second interpremolar width, intermolar width and arch length. Results Incisor irregularity for both the conventional and self-ligating systems increased significantly during the 6-month retention period. For both systems, the overall changes in intercanine width, first interpremolar width, and intermolar width from T1 to T4 were not statistically significant. No statistically significant differences in incisor irregularity or arch dimensions were recorded during the 6-month retention period between the self-ligating and conventional systems. The changes in intercanine width, first interpremolar width, second interpremolar width, intermolar width, and arch length during the 6 months in both systems were less than 1 mm. Conclusion During the 6-month retention period, the stability of self-ligating system and conventional system after orthodontic treatment of mild to moderate crowded malocclusion with mandibular arch is similar.
  • Clinical evaluation of tympanoplasty based on tragus cartilage perichondrium complex micro flap explantation in continuous perfusion mode otoendoscopy
    CHEN Guo, LIANG Siyu, XIAO Qizhen, SHI Wei, YANG Yanqiong
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 59-63.
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    Objective To evaluate the clinical efficacy of tympanoplasty for repairing tympanic membrane perforation using a microflap exoplantation technique auricle tragal cartilage-perichondrium composite during continuous perfusion mode otoscopic surgery. Methods A total of 100 patients (102 ears) undergoing tympanoplasty admitted to the Otorhinolaryngology Department of Jianyang People's Hospital between January 2021 and January 2024 were selected as study subjects. Using simple randomization, patients were divided into an observation group (microflap exoplantation technique, n=50) and a control group (conventional skin flap exoplantation technique, n=50). Both groups were repaired with tragal cartilage-perichondrium composite and underwent surgery via otoscopy. Postoperative follow-up assessments were conducted on operation duration, intraoperative blood loss, tympanic membrane healing rate, and hearing recovery. Results The observation group showed significantly better outcomes in terms of operation duration, intraoperative blood loss, and hospital stay compared to the control group. Both groups exhibited significant improvements in hearing indices at 6 months postoperatively compared to preoperative levels, but there were no significant differences between the two groups. The tympanic membrane healing rates in the observation group at 1 and 6 months postoperatively were 98.04% and 100.00%, respectively, with no statistically significant difference compared to the control group. Conclusion Continuous perfusion mode otoscopic surgery combined with the microflap exoplantation technique using tragal cartilage-perichondrium composite is an efficient and safe tympanic membrane repair technique that offers advantages such as shortened operation duration, reduced blood loss, and improved postoperative recovery quality, making it suitable for widespread clinical application.
  • Observation of the value of DWI-MRI combined with ultrasound in diagnosing placental implantation
    FENG Xinyu, ZHANG Fengli, CHEN Chuchu
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 64-68.
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    Objective To evaluate the value of DWI-MRI (diffusion-weighted magnetic resonance imaging) combined with ultrasound in the diagnosis of placental implantation. Method This study included 102 pregnant women who were admitted to Changzhou Maternal and Child Health Care Hospital from January 2021 to December 2023 and were suspected of having placental implantation. All patients underwent ultrasound examination and DWI-MRI scan. Using the results of ultrasound DWI-MRI as a diagnostic tool, compare the results of DWI-MRI combined with ultrasound and pathological diagnosis of placental implantation with postoperative pathological results, and evaluate its diagnostic accuracy. Result The pathological diagnosis of 102 pregnant women suspected of placental implantation showed 86 positive cases and 16 negative cases. Ultrasound testing identified 75 out of 86 positive cases as positive and mistakenly judged 13 negative cases as positive, while DWI-MRI combined with ultrasound identified 85 out of the same positive cases and misdiagnosed 7 negative cases as positive. The combination of DWI-MRI and ultrasound showed higher sensitivity (98.84%) and specificity (56.25%), with a positive predictive value of 92.39% and a negative predictive value of 76.47%, while the sensitivity of the ultrasound group was 87.21%, specificity was 18.75%, positive predictive value was 85.23%, and negative predictive value was 21.43%. In addition, the diagnostic accuracy of the combined group was 92.16%, while that of the ultrasound group was 76.47%. Ultrasound shows an increase in placental thickness, heterogeneous echoes, blurred interfaces, and abnormal blood flow patterns, while DWI-MRI shows high signal intensity of the placenta, interruption of the uterine wall, and heterogeneous signal enhancement. Both methods can effectively depict abnormal blood vessels. In the classification diagnosis of placental implantation, 45 cases were correctly diagnosed by ultrasound, 24 cases were adhesive type, 6 cases were penetrating type, and 3 cases were normal; The combination of DWI-MRI and ultrasound is more accurate, with 51 cases diagnosed as implanted type, 27 cases as adhesive type, 7 cases as penetrating type, and 9 cases as normal. Conclusion The combination of DWI-MRI and ultrasound has high diagnostic accuracy in the diagnosis of placental implantation and can serve as an effective tool for clinical diagnosis of placental implantation. This combined diagnostic method helps to identify high-risk pregnant women in advance, enabling appropriate clinical interventions and reducing maternal and infant risks.
  • Analysis of the incidence and susceptibility factors of pulmonary infection in patients with spinal injury and paraplegia
    YANG Kaichao, HUANG Jianying, GENG Lei, WANG Xue
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 69-72.
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    Objective To analyze the incidence and susceptibility factors of pulmonary infection in patients with spinal injury and paraplegia. Methods The clinical data of 106 patients with spinal injury and paraplegia admitted to the hospital from January 2023 to December 2024 were retrospectively analyzed. Patients enrolled were divided into the pulmonary infection group (n=42) and the non-pulmonary infection group (n=64) according to whether they had developed pulmonary infection. Univariate and multivariate logistic regression analyses were used to screen the risk factors for pulmonary infection in patients with spinal injury and paraplegia. ROC curve was used to evaluate the predictive value. Results Logistic regression analysis results showed that age (OR=1.103, 95%CI: 1.031-1.181), high paraplegia (OR=7.288, 95%CI: 1.246-42.643), MNA-SF score (OR=0.650, 95%CI: 0.489-0.862), and mechanical ventilation (OR=3.997, 95%CI: 1.356-11.778) were independent risk factors for pulmonary infection in patients with spinal injury and paraplegia. ROC analysis showed that the AUC value of combined prediction with the four factors was 0.870 and Youden index was 0.653. The values were significantly higher than those of separate prediction with each factor (Z=4.002, 4.088, 3.538, 4.116). Conclusion The incidence of pulmonary infection is relatively high in patients with spinal injury and paraplegia. Advanced age, high paraplegia, low MNA-SF score, and mechanical ventilation are susceptible factors for pulmonary infection in patients with spinal injury and paraplegia.
  • Identification of a prognostic risk signature and immune features in hepatocellular carcinoma
    HUANG Shiqiong, HE Gefei, HUANG Juanjuan
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 73-81.
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    Objective To screen signature genes and construct an effective prognostic model for hepatocellular carcinoma (HCC), ultimately providing a more accurate evaluation on prognosis for patients with HCC. Method DEGs between carcinoma tissue and normal tissue were identified based on the cancer genome atlas (TCGA) and gene expression omnibus (GEO) database. The signature genes for HCC were screened via univariate cox regression and the least absolute shrinkage and selection operator-cox regression. The multivariate cox analysis was used to construct a monogram and a prognostic risk score model. Based on this model, the patients were grouped into low-risk group and high-risk group. Enrichment analysis and gene set enrichment analysis was performed on DEGs between these two subgroups. CIBERSORT algorithm was employed to evaluate the level of tumor-infiltrating immune cells. Results A total of 5 signature genes, including CCNB2, CCNB1, NDC80, RRM2 and MCM2, were identified from 613 overlapping DEGs in HCC. The prognostic risk score model was constructed based on these candidate genes. Patients in high-risk group had a significantly shorter OS compared with those in low-risk group. Univariate and multivariate cox regression analysis showed that both the risk scores from prognostic model and tumor stages were independent prognostic factors for HCC patients. Furthermore, based on risk scores and clinical information, the nomogram plot was constructed. It was shown that high-risk group was related to immune reactions according to gene set enrichment analysis. Further immune-infiltrating analysis revealed that the expression of the signature genes was positively correlated with the level of activated CD4+T cell memory but negatively related with the level of resting CD4+T cell memory. Conclusion This study screened 5 signature genes and constructed a prognostic risk model, providing novel insights and strategies for the prognosis and therapy of HCC.
  • Clinical efficacy and safety of Tangning Tongluo tablets combined with Tangning Tongluo cream in the treatment of type 2 diabetic foot ulcer
    HU Renyue, SUN Qi, GU Haiyun, PAN Yujie, ZHOU Ranran, SHI Rongfeng, LIANG Guangwu, XU Tongtong, CHEN Jing, SUN Tiantian, TANG Zhuqi, XIA Wen, GU Yunjuan
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 82-88.
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    Objective To evaluate the clinical efficacy and safety of Tangning Tongluo tablets and Tangning Tongluo cream in patients with type 2 diabetic foot ulcer. Methods 100 patients with diabetic foot were randomly divided into 5 groups: control group (routine treatment), oral group (routine treatment+Tangning Tongluo tablet), external application group (routine treatment+Tangning Tongluo ointment), combined group (routine treatment+Tangning Tongluo tablet+Tangning Tongluo ointment) and high-dose oral group (routine treatment+Tangning Tongluo tablet). Finally, 5 cases were excluded. The outcomes of patients treated for 1 month and 3 months, the changes of serological indexes, and the ulcer outcomes with Wagner grade<4 or Wagner grade=4 were compared and observed, and the adverse reactions during the whole treatment period were recorded. Results There was no significant difference in baseline data between the groups. One month after treatment, there was no significant difference in the healing rate between the general population and patients with Wagner grade<4. Three months after treatment, there were significant differences in the healing rate between the general population and patients with Wagner grade<4, and the healing rates of oral group, external application group, combined group and high-dose oral group were significantly higher than those of the control group. Serological indicators, 3 months after treatment, the level of white blood cells in the high-dose oral group decreased significantly; Except for the control group, the neutrophil count in other groups decreased significantly, and the levels of alanine aminotransferase, aspartate aminotransferase, albumin and creatinine in each group did not change significantly before and after treatment. Safety evaluation showed that the patients did not have any adverse reactions during the whole treatment. Conclusion The combined use of Tangning Tongluo Tablet and Tangning Tongluo Cream can effectively reduce wound infection and promote wound healing. Its long-term clinical efficacy is significantly higher than that of conventional treatment, and its safety is good, which improves the clinical prognosis of patients and has high clinical application value.
  • Specificity analysis and clinical value assessment of specific serum microRNAs in the early diagnosis of B-Cell non-Hodgkin lymphoma
    QU Jiaoyan, ZHANG Yue, HUO Hongjia, ZHOU Hebing
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 89-93.
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    Objective to explore the specificity and clinical value of specific serum microRNAs (miRNAs) in the early diagnosis of B-cell non-Hodgkin's lymphoma (B-NHL). Methods A total of 115 patients with B-NHL admitted to our hospital from January 2020 to January 2023 were selected as the B-NHL group, and another 100 healthy individuals who underwent physical examinations during the same period were selected as the control group. The expression levels of specific miRNAs (including miRNA-21 and miRNA-155) in the serum of both groups were detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR). The relationship between the expression levels of specific miRNAs and the clinical pathological characteristics of B-NHL patients was analyzed. The diagnostic efficacy of specific miRNAs for B-NHL was analyzed by receiver operating characteristic curve (ROC). Results The relative expression levels of miRNA-21 and miRNA-155 in the serum of the B-NHL group were both higher than those of the control group (P<0.05). There are significant differences in the relative expression levels of miRNA-21 and miRNA-155 in the serum of B-NHL group patients with different Ann Arbor stages, B symptoms, International Prognostic Index (IPI) scores, β2-microglobulin (β2-MG) levels and lactate dehydrogenase (LDH) levels (P<0.05). The results of the ROC curve showed that the AUCs of serum miRNA-21 and miRNA-155 alone in the diagnosis of B-NHL were 0.796 and 0.842, respectively, the sensitivities were 73.30% and 78.29%, respectively, and the specificities were 78.39% and 85.01%, respectively. The positive predictive values were 80.19% and 86.42% respectively, and the negative predictive values were 72.23% and 75.90% respectively. The AUC of the combined detection for diagnosing B-NHL was 0.927, which was higher than that of the single detection, with a sensitivity of 88.35%, a specificity of 90.01%, a positive predictive value of 92.22%, and a negative predictive value of 85.73%. Conclusion serum miRNA-21 and miRNA-155 are highly expressed in patients with B-NHL and are closely related to clinical pathological features. The combined detection can improve the early diagnostic efficiency of B-NHL and can also be used to evaluate the prognosis of patients.
  • Effect of a precision psychological capital intervention on caregiver burden in patients with Alzheimer's disease
    HAO Rongrong, DING Lirong, LI Xianghua, ZHANG Yanxia, NIU Lanmin
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 94-101.
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    Objective The aim of this study is to analyze the impact and significance of Alzheimer's disease (AD) patients on caregiver burden, psychological status and quality of life from the perspective of Precision Psychological Capital Intervention (P-PCI). Methods Patients diagnosed with AD and their caregivers from our hospital between May 2019 and October 2022 were enrolled in the study, and all patients underwent conventional treatment for AD. The patients and their caregivers were divided into a control group (n=37), who underwent usual care, and an observation group (n=41), who underwent P-PCI on top of that, using a randomized numerical table. The psychological status of the carers was assessed by the positive psychological-capital questionnaire (PPQ), the readiness of the carers was assessed by the caregiver preparedness scale (CPS), the caring capacity of the carers was assessed by the family caregiver task inventory (FCTI), the carer burden was assessed by the caregiver burden inventory (CBI), the carer's social support was assessed by the social support rating scale (SSRS), and the WHOQOL-BREF assessed patient/carer quality of survival. Results After the intervention, compared with the Control group, the observation group: (1) showed a significant upward trend in PPQ dimensions: PPQ dimensions: self-efficacy, optimism, resilience, and hope, as well as the total score; (2) showed an upward trend in CPS, and an even more pronounced downward trend in FCTI; (3) showed a significant upward trend in CBI dimensions: time-dependent, developmentally limited, physical, social, and emotional load scores, as well as a significant downward trend in the total score; (4) showed a significant upward trend in all SSRS dimensions: a more pronounced upward trend in the objective support, subjective support, and utilisation of support scores, as well as in the total score; (5) showed a significant upward trend in WHOQOL-BREF score in caregiver's and patient's of all dimensions: trend in the physiological, psychological, social relationship, and environmental were more pronounced upward. Conclusion P-PCI can reduce the burden of caregiving and improve the quality of life of caregivers of patients with AD by effectively enhancing their psychological capital, which in turn leads to improved quality of life for patients.
  • Impact evaluation of auricular acupressure tablets and tongjiao limian patches on cancer patients' quality of life
    SUN Yu, SUN Peiyang, ZHU Chaolin, YOU Min, WU Yong, ZHOU Xianyang, ZHANG Liufeng, ZHU Yueping
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 102-105.
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    Objective To investigate the influence of auricular acupressure tablets combined with Tongjiao Limian patches on enhancing the quality of life for cancer patients. Methods The research retrospectively assessed 80 cancer patients hospitalized from October 2022 to September 2024. These patients were divided into two groups: a control group receiving conventional Western medicine and a combination group receiving additional auricular acupressure tablets and Tongjiao Limian patches, with 40 patients in each group. The study compared clinical outcomes, quality of life and health-related indicators before and after treatment [including karnofsky performance status (KPS) score, self rating anxiety scale (SAS) score, pittsburgh sleep quality index (PSQI) score], serum neurotransmitters [5-hydroxytryptamine (5-HT), brain-derived neurotrophic factor (BDNF), neuropeptide Y (NPY)] levels, and adverse reactions between the two groups. Results The combination group demonstrated a higher total effective rate (82.50%) than the control group (57.50%). Post - treatment, both groups showed elevated KPS scores and serum neurotransmitter levels, but the combination group had significantly higher values. SAS and PSQI scores decreased in both groups, yet the combination group had lower scores. Additionally, the combination group experienced a lower adverse reaction rate (10.00%) compared to the control group (32.50%). Conclusions The combined use of auricular acupressure tablets and Tongjiao Limian patches effectively enhances cancer patients' quality of life by improving sleep, alleviating psychological distress, and regulating neurotransmitter levels, with a satisfactory safety profile. These findings suggest.
  • Analysis of the relationship between disease uncertainty, mental resilience and pregnancy stress in pregnant women with pregnancy-induced hypertension
    YAN Wenye, YIN Yin, HUANG Mei, XU Jialu, SUN Dandan
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 106-109.
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    Objective To analyze the relationship between disease uncertainty, mental resilience and pregnancy stress in pregnant women with pregnancy-induced hypertension. Methods A total of 124 pregnant women with gestational hypertension who visited the First Affiliated Hospital of Nanjing Medical University from February 2023 to June 2024 or had regular antenatal check-ups were selected as the study objects. Mishel uncertainty in illness scale for adults (MUIS-A), connor-daviddson resilience scale (CD-RISC) and pregnancy pressure scale (PPS) were used to investigate. Multiple linear regression analysis was used to analyze the influencing factors of pregnant women's disease uncertainty. Results The scores of MUIS-A, CD-RISC and PPS were (86.58±7.63), (60.26±5.74) and (69.37±5.24) respectively. Pearson correlation analysis showed that MUIS-A was negatively correlated with CD-RISC (P<0.05). MUIS-A was positively correlated with PPS (P<0.05). Multiple linear regression analysis showed that CD-RISC score, PPS score, age and adverse pregnancy history were all influencing factors of disease uncertainty in pregnant women (P<0.05). Conclusion The uncertainty of pregnancy-induced hypertension is related to psychological resilience and pregnancy stress.
  • Influence of low-temperature plasma radiofrequency ablation combined with collagenase injection on cervical pain and function in patients with cervical spondylotic radiculopathy
    LI Sheng, WANG Dabin
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 110-114.
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    Objective To investigate the influence of low-temperature plasma radiofrequency ablation (TCAR) combined with collagenase injection on cervical pain and function in patients with cervical spondylotic radiculopathy (CSR). Methods The clinical data of CSR patients admitted from September 2021 to September 2023 were retrospectively analysed, and according to the treatment method of the patients, 46 cases were randomly selected from those who underwent collagenase injection treatment to be included in the control group, and 46 cases were randomly selected from those who underwent TCAR combined with collagenase injection treatment to be included in the combined group. The clinical efficacy at 12 months after treatment and pain degree [visual analogue scale (VAS)], cervical spine function (NDI), cervical spine activities, serum indicators [cyclooxygenase-2 (COX-2), neuropeptide Y (NPY), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α)] at different time points after treatment were compared between groups. Results After 12 months of treatment, the total effective rate of clinical efficacy in combined group was higher than that in control group. The VAS、NDI score in the two groups at 6 months and 12 months after treatment was reduced compared with that before treatment, and the score in the same group at 12 months after treatment was lower than that at 6 months after treatment, and the score at each time point after treatment was lower in combined group than that in control group. At 6 months after treatment, the cervical spine activities were increased in the two groups, and the indicators in combined group were higher compared to control group. The levels of COX-2, NPY, IL-6 and TNF-α in both groups were decreased, and the above levels were lower in combined group than those in control group. Conclusion The application of TCAR combined with collagenase injection can significantly improve the severity of cervical pain in patients with CSR, restore the cervical spine function, and help to enhance the clinical efficacy of patients.
  • A randomized controlled study of muscle energy technology combined with 3M physical therapy to improve mandibular motor function in patients with temporomandibular joint disorder
    ZHANG Xuejiao, SU Ning, TANG Chao, MA Cheng
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 115-119.
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    Objective A randomized controlled study of muscle energy technology combined with 3M physical therapy to improve mandibular motor function in patients with temporomandibular joint disorder. Methods The study subjects consisted of 100 patients with temporomandibular joint disorders admitted to our hospital from October 2022 to August 2024. They were randomly divided into a control group and an observation group using a random number table, with 50 cases in each group. Both groups received 3M physical therapy, while the observation group additionally received muscle energy technique treatment. Both groups underwent treatment for 4 weeks, and the therapeutic effects of muscle energy technique combined with 3M physical therapy on temporomandibular joint disorders were assessed. Results After treatment, the observation group had higher maximum mouth opening (MMO) and pressure pain threshold (PPT) than the control group. The observation group also had lower disability index (DI), pain index (PI), clinical management index (CMI), visual analogue scale (VAS), and mandibular function impairment questionnaire (MFIQ) compared to the control group. The total effective rates of the observation group patients were 96.00%, significantly higher than the 78.00% of the control group patients. Conclusion Muscle energy technique combined with 3M physical therapy can significantly improve clinical symptoms and enhance functional levels in patients with temporomandibular joint disorders. Compared to the application of 3M physical therapy alone, the combined treatment is more effective and worth promoting.
  • Correlation analysis of platelet count and routine laboratory parameters with liver fibrosis severity in patients with chronic hepatitis B virus infection
    YANG Zhijuan, HAN Huanqin
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 120-125.
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    Objective To investigate the correlation between routine laboratory indicators and the severity of liver fibrosis in patients with chronic hepatitis B virus (HBV) infection. Methods This retrospective study analyzed clinical data from 202 patients with chronic HBV infection who were admitted to the Department of Infectious Diseases at Maoming Clinical Medical College, Affiliated Hospital of Guangdong Medical University, between January 2023 and December 2023. Based on liver stiffness measurement (LSM) values, patients were categorized into fibrosis stages F0-F1(n=84), F2(n=56), F3(n=48), and F4(n=14). Routine blood, coagulation, and liver function indicators were measured and their correlations with liver fibrosis severity were analyzed. Receiver operating characteristic (ROC) curves were used to assess the predictive value of individual and combined indicators in evaluating fibrosis severity, and diagnostic thresholds and area under the curve (AUC) values were determined. Results With the progression of liver fibrosis, levels of platelet count (PLT), prothrombin activity (PTA), albumin (ALB), and cholinesterase (CHE) gradually decreased, reaching their lowest levels at stage F4. In contrast, levels of total bile acid to platelet ratio (TRP), AST-to-platelet ratio index (APRI), total bilirubin (TBIL), direct bilirubin (DBIL), and gamma-glutamyl transferase (GGT) increased with fibrosis stage. Pearson correlation analysis showed that TRP, APRI, TBIL, DBIL, and globulin (GLB) were positively correlated with fibrosis severity, while PLT was negatively correlated. ROC curve analysis revealed that the combination of 13 routine indicators yielded an AUC of 0.930, with a sensitivity of 0.894 and a specificity of 0.923, significantly outperforming any single indicator, indicating high predictive value for the combined assessment. Conclusion Routine laboratory indicators—including blood counts, liver function, and coagulation parameters—are closely associated with the severity of liver fibrosis in patients with chronic HBV infection. Among them, PLT shows the most significant decline in advanced fibrosis. The combined use of indicators such as TRP and APRI can effectively improve the accuracy of fibrosis prediction and holds significant clinical utility.
  • Clinical value of a multiple regression model based on multimodal ultrasound for predicting luminal and non-luminal breast cancer subtypes
    KANG Jinjuan, HUANG Ling, YU Peng, QIU Jingjing, JIANG Yuyang, WANG Jinrong
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 126-133.
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    Objective To analyze the multimodal ultrasound features of different breast cancers (BC) and explore their diagnostic value in molecular subtyping. Methods This study included 120 BC patients admitted to our hospital, with pathological diagnosis as the gold standard. Data from ultrasonic imaging (US), strain ultrasound elastography (SUE), contrast-enhanced ultrasound (CEUS), and relevant clinical information were collected. Chi-square tests were used to screen for significantly different features between groups, and two-way stepwise logistic regression analysis was performed to construct prediction models for molecular subtypes (Luminal vs. non-Luminal). Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic efficacy of single-modal and multimodal combined ultrasound techniques. Results US examination showed that Luminal subtypes (LA/LB) were significantly correlated with lymph node morphology, enhanced range, and internal echo, while non-Luminal subtypes (HER2+/TNBC) were significantly associated with lesion morphology, calcification, lymph node morphology, and margin. SUE results indicated that all molecular subtypes were significantly correlated with elasticity scores. CEUS analysis revealed that both Luminal and non-Luminal subtypes were significantly associated with enhancement defects and enhanced range. The area under the ROC curve (AUC) of the constructed prediction models was 0.859 for Luminal subtype differentiation and 0.947 for non-Luminal subtype discrimination. Efficacy comparison showed that the multimodal ultrasound combined model exhibited superior diagnostic performance compared with single-modal models. Conclusion Multimodal ultrasound can effectively distinguish BC molecular subtypes, and the combined model significantly improves diagnostic efficacy, providing an imaging basis for clinical precise subtyping.
    • Pharmacy
  • Preparation and characterisation of a novel nanoparticle for delivery of oxaliplatin
    LI Lewei, LI Jinhui, PENG Xingcheng, LIU Yi, TAO Xiaojun, HE Chunlian
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 134-141.
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    Objective Design and preparation of a novel poly (lysine) amphiphilic polymer nanodelivery system for oxaliplatin (OXA) delivery to achieve improved efficacy and target site drug release rate. Methods The mPssPC polymers were prepared by chemical synthesis, and the successful synthesis of the polymers was confirmed by infrared spectroscopy and nuclear magnetic resonance hydrogen spectroscopy. The mPssPC NPs and mPssPC-OXA NPs were prepared by physical embedding and self-assembly, and the physicochemical properties of the nanoparticles were characterized by dynamic light scattering analysis, transmission electron microscopy observation, stability test and in vitro drug release assay. The cytotoxicity, cellular uptake, and immune escape effects of mPssPC-OXA NPs were tested by in vitro cellular assays. Results mPssPC NPs and mPssPC-OXA NPs were successfully prepared. mPssPC NPs had a small particle size (190.77±2.91 nm), positive charge (43.20±0.72 mV), and excellent stability. mPssPC-OXA NPs showed a spherical shape with regular shape under transmission electron microscopy, and the drug release of mPssPC-OXA NPs was characterised by slow release and GSH-sensitive drug release. In in vitro cellular studies, mPssPC-OXA NPs were effectively taken up by HCT-116 cells and significantly inhibited tumor cell activity, and also effectively reduced phagocytic clearance of nanoparticles by RAW264.7 cells. Conclusion The mPssPC-OXA NPs constructed with polylysine as the basic backbone promoted drug uptake in tumor cells, which significantly increased cytotoxicity. Meanwhile, mPssPC-OXA NPs could achieve immune escape and more and faster drug release in a high glutathione concentration environment, achieving the effect of increasing the drug concentration at the target site.
    • Preventive Medicine
  • The research on the current situation and influencing factors of primary school students' mental health under the background of “double reduction”
    HUANG Ying, XU Jinghui, OUYANG Haijing, LUO Fang, XIANG Zhihao, HAN Zixuan, XU Minhui, Ma Xiang, WU Bohan, ZHANG Hao, XIE Peiwei, YANG Jianfeng, QIN Lulu
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 142-148.
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    Objective This study was conducted in Changsha, Hunan Province, to assess the current mental health status of primary school students and analyze its influencing factors. At the same time, it explores the relationship between primary school students’ mental health, sleep quality and learning burnout, providing a theoretical basis for further improving and enhancing the mental health of primary school students. Methods Using the method of multi - stage random cluster sampling, 7 schools were randomly selected as research sites in Changsha City. A total of 1073 primary school students were finally selected. Self - designed questionnaires were used to investigate the general demographic data of primary school students. The Kessler 6 Rating Scale (K6) was applied to conduct a questionnaire survey on the mental health of primary school students. At the same time, the sleep quality and learning burnout of primary school students were measured. Results The average mental health score of the subjects was 4.450±4.484 points. There were 75 people (6.9%) with a score of 13 points or above, indicating a relatively high risk of mental disorders. Among them, the score of question 3(uneasy or irritable) was the highest, at 1.998±1.048 points, and the score of question 5(It's laborious to do everything) was the lowest, at 1.512±1.875 points. Mental health was significantly positively correlated with sleep quality and also significantly positively correlated with learning burnout. Multiple linear regression analysis showed that sleep quality, and learning burnout were independent risk factors for mental health problems among primary school students, Academic burnout not only directly affects students’ mental health, but also indirectly influences it through sleep quality, with the mediating effect value being 30.5%. Conclusion The mental health level of primary school students in Changsha is above the medium level. Sleep quality and learning burnout are influencing factors of primary school students' mental health. Mental health is significantly positively correlated with sleep quality and learning burnout, Learning burnout positively predicts a decline in mental health, and sleep quality plays a mediating role in the relationship between learning burnout and mental health.
  • The current status and influencing factors of overweight and obesity among preschool children in Fuzhou City
    WANG Xiaomei, LI Guobo, LIU Guihua
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 149-155.
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    Objective To investigate the prevalence of overweight and obesity among preschool children in Fuzhou City, Fujian Province, and compare it with the national average and other cities. To analyze the main influencing factors of overweight and obesity among preschool children from aspects such as age, gender, screen exposure, dietary intake, sleep patterns, physical activity, grandparental feeding, parents’ weight, and parents’ educational level. To provide practical prevention and control suggestions and a basis for the education and disease control departments in Fujian Province in the prevention and health care of childhood obesity and related research. Methods A cluster random sampling method was used to include 3 811 preschool children aged 3-6 years as the research subjects. Descriptive statistics, chi-square test, and multivariate logistic regression analysis were conducted using SPSS 26.0 software. Results 1) The total prevalence of overweight among preschool children in Fuzhou City was 12.99% (14.25% for boys and 11.59% for girls), and the total prevalence of obesity was 7.01% (8.12% for boys and 5.76% for girls). There was a statistically significant difference in the prevalence of obesity between boys and girls. 2) The prevalence of overweight among children in large, medium, and small classes ranged from 12.38% to 14.20%, and the prevalence of obesity ranged from 6.67% to 7.37%, with no statistically significant differences. 3) Multivariate logistic regression analysis showed that gender (higher risk for boys), parental overweight/obesity (especially mothers), excessive screen exposure (>1 h/d), high-sugar and high-fat diet (≥3 times/week), insufficient physical activity (<1 h/d), excessive sleep time (>13 h/d), and grandparental feeding were the main influencing factors. Conclusion The prevalence of overweight and obesity among preschool children in Fuzhou City is relatively high, higher than the national average and some economically underdeveloped areas in the central and western regions, and comparable to that in other economically developed areas. Gender (higher risk for boys), parental overweight/obesity (especially mothers), excessive screen exposure (>1 h/d), high-sugar and high-fat diet (≥ 3 times/week), insufficient physical activity (<1 h/d), excessive sleep time (>13 h/d), and grandparental feeding are the main influencing factors.
    • Nursing Science
  • Application of discharge preparation plan based on behavior change wheel theory in ischemic stroke patients with coexisting diseases
    TAO Qiuhong, GAO Wenfang, ZHOU Lianfa, SHI Qi
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 156-161.
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    Objective To use behaviour change wheel theory (BCW) as the theoretical basis to explore the effect of discharge preparation plan constructed from three aspects: ability, motivation and opportunity in patients with ischemic stroke and multi-morbidity. Methods Sixty cases of ischemic stroke multimorbidity patients admitted to the neurosurgery department of a hospital in Anhui Province from December 2022 to November 2023 were selected as the study subjects and were randomly divided into a control group and an observation group, with 30 cases in each group; the control group implemented the hospital routine nursing care, and the observation group implemented the discharge readiness program based on the BCW theory. Differences in discharge readiness, medication adherence, and quality of life between the two groups were compared at the time of admission, 2 weeks after discharge, and 4 weeks after discharge. Results The scores of all dimensions of discharge preparation in the observation group were higher than those of the control group at the time of discharge. Compared with the time of admission, the scores of medication adherence after discharge were significantly higher in the observation group, and the scores of the observation group were higher than those of the control group. The scores of all dimensions of quality of life in the observation group were higher than those of the control group after 4 weeks of discharge. Conclusion A discharge readiness program based on the BCW theory improves patients' readiness for discharge, medication adherence, and quality of life, and helps to facilitate the long-term rehabilitation management of this patient population.
  • Based on the three-dimensional quality structure model, an evaluation index system of stroke service ability in grassroots medical institutions was established
    DENG Peipei, LI Meizhi, LIAO Sisi, SHI Xiaomao, ZHANG Ke, ZHU Kerong
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 162-168.
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    Objective Establish a scientific, objective, and comprehensive evaluation index system for stroke service capabilities of primary healthcare institutions. Methods Guided by the theory of three-dimensional quality structure model, this study employed literature review, group discussion, two rounds of Delphi expert consultation, and the Analytic Hierarchy Process (AHP) to screen indicators, determine weights, and establish an evaluation index system for stroke service capabilities in primary healthcare institutions. Results The recovery rate was 100%, the expert authority coefficient was 0.878 and 0.889, and the Kendall's tau-distance coefficients in the first round were 0.299, 0.513, and 0.405, respectively, and those in the second round were 0.299, 0.437, and 0.413, respectively, all with statistical significance (P<0.001). The final evaluation index system for stroke service capacity in primary medical institutions includes 3 first-level indexes (population screening management, stroke organizational management, and stroke diagnosis and treatment management), 11 second-level indexes, and 51 third-level indexes. Conclusion The evaluation index system for stroke service capabilities in primary healthcare institutions that has been established provides a reference for objectively assessing the stroke service capabilities of primary healthcare institutions, continuously optimizing processes, standardizing behaviors, and making continuous improvements.
  • Influence of multidimensional nursing based on developmental care on pulmonary function, psychomotor function and intelligent development of neonatal pneumonia
    WU Xue, LI Xiaoyu, WEI Lirong, ZHANG Guilan, MU Xiaolan, LI Hongmei, LUO Yanyan, WANG Jurong, ZHANG Chunhua
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 169-173.
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    Objective To explore the influence of multidimensional nursing based on developmental care on the pulmonary function, psychomotor function and intelligent development of neonatal pneumonia. Methods 160 children with neonatal pneumonia who were born in the hospital were selected from February 2020 to February 2024, and were randomized into observation group and control group with 80 cases in each group. The children in the control group were given routine nursing, while the children in the observation group were given multidimensional nursing based on developmental care on the basis of the control group. The clinical respiratory indicators, blood gas indicators, psychomotor function, intelligent development and nursing satisfaction of children's family members before and after intervention were compared between groups of children. Results After intervention, the respiratory rate and arterial partial pressure of carbon dioxide (PaCO2) in the two groups were significantly reduced compared with those before intervention, and the above indicators in observation group were significantly lower. The respiratory peak volume ratio, respiratory peak time ratio, arterial partial pressure of oxygen (PaO2) and blood oxygen saturation (SpO2) were significantly higher in the two groups after intervention than those before intervention, and the above indicators were significantly higher in observation group. There were no significant differences in Psychomotor Development Index (PDI) score and Mental Development Index (MDI) score before and after intervention in control group. After intervention, the scores of PDI and MDI were significantly elevated, and the scores in observation group were significantly higher than those in control group. Compared with family members of children in control group, the nursing satisfaction of family members in observation group was significantly higher. Conclusion The application of multidimensional nursing based on developmental care in children with neonatal pneumonia is helpful to promote the psychomotor function and intelligent development, accelerate the recovery of pulmonary function and improve the blood gas indicators. The family members of children are more satisfied with this nursing model.
  • The efficacy of perioperative nursing based on the FTS (Fast-Track Surgery) paradigm in managing patients during the perioperative period of hepatic artery chemoembolization for hepatocellular carcinoma
    XUAN Yajun, FANG Yuanyuan, WANG Quanlan, ZHANG Chao
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 174-179.
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    Objective To evaluate the efficacy of perioperative nursing based on the fast track surgery (FTS) concept in patients with liver cancer undergoing transcatheter arterial chemoembolization (TACE). Methods This prospective study included 103 liver cancer patients scheduled for TACE between January 2022 and June 2024. They were randomly assigned to an observation group (51 patients) and a control group (52 patients). During the study, one patient in the observation group and two in the control group were lost to follow-up, leaving 50 patients in each group for final analysis. The control group received standard care, while the observation group was managed with FTS-based nursing. Comparisons were made between the groups for perioperative liver function parameters [total bilirubin (TBIL), albumin (ALB), alkaline phosphatase (ALP)], inflammatory and stress markers [C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6)], pain intensity [via visual analogue scale (VAS)], and psychological status [using Self-Rating Anxiety Scale (SAS) and Self-Rating Depression Scale (SDS)] before and after intervention. Postoperative recovery indicators and complication rates were also assessed. Results Three days postoperatively, the observation group exhibited lower TBIL and ALP levels and a higher ALB level compared to the control group. The observation group also showed shorter times to first water intake, eating, and defecation. Both groups demonstrated reduced SAS and SDS scores post-intervention, with the observation group having lower scores than the control group. For VAS scores and levels of CRP, TNF-α, and IL-6, the observation group had lower values than the control group on days 1, 2, and 3 after surgery. Three days after surgery, the incidence of complications such as nausea and vomiting, constipation and abdominal distension was significantly lower in the observation group than in the control group. Conclusion Perioperative nursing guided by the FTS concept can enhance liver function in TACE patients, mitigate inflammatory and stress responses, alleviate anxiety and depression, promote recovery, and reduce complications.
  • Research on the application effect of evidence based detail nursing combined with facial muscle training in patients with facial neuritis
    HUANG Manhua, WU Yiqing, LUO Lixia, HE Yingyi, QIU Honglan, YANG Jialin
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 180-184.
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    Objective To investigate the application of detailed nursing care based on evidence-based concepts combined with facial muscle training in patients with facial neuritis. Methods Eighty patients with facial neuritis who visited the Department of Neurology in our hospital from January 2023 to June 2024 were selected as study subjects. They were divided into two groups of 40 patients each using the envelope method. The control group received conventional nursing interventions, while the observation group received facial muscle training combined with detailed nursing interventions based on evidence-based concepts in addition to the conventional interventions. The intervention effects of the two groups were compared. Results After 2 weeks of intervention, the Facial Nerve Function Assessment Rating Scale (House-Brackmann) scores in the observation group were better than those in the control group (P<0.05). The Facial Disability Index (FDI) scores in the observation group were significantly better than those in the control group after 2 weeks of intervention (P<0.05). Additionally, the Hamilton anxity rating scale (HAMA) and Hamilton depression rating scale (HAMD) scores in the observation group were lower than those in the control group after 2 weeks of intervention (P<0.05). Furthermore, nursing satisfaction in the observation group was significantly higher than that in the control group after 2 weeks of intervention (P<0.05). Conclusion Detailed care based on evidence-based concepts combined with facial muscle training can effectively promote the recovery of facial nerve function and reduce pain in patients with facial neuritis. This combination has a positive impact on eliminating patients’ negative psychological emotions, improving nursing satisfaction, and enhancing quality of life.
  • Development of a questionnaire for the prevention and control of respiratory infectious diseases among community residents and test of its reliability and validity
    ZOU Huangjue, WANG Weihong, OU Lu, LU Yanmei
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 185-191.
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    Objective To develop a questionnaire on the prevention and control literacy of respiratory infectious diseases for community residents and evaluate its reliability and validity. Methods Based on the theory of knowing, believing, and doing, a preliminary pool of questionnaire entries was drawn up through literature analysis. The Delphi method was applied to conduct 2 rounds of correspondence with 16 experts, and the questionnaire content was adjusted according to the experts’ opinions to form the first draft. Using stratified random sampling method, 433 community residents in Changsha City were selected in October 2024 to conduct the survey, and the questionnaire was subjected to item analysis and reliability test. Results The questionnaire on the prevention and control literacy of respiratory infectious diseases for community residents includes three dimensions and 36 items. The three dimensions are prevention and control knowledge, prevention and control skills and behaviors, and the ability to obtain and evaluate prevention and control information. Both the item analysis and the reliability and validity of the questionnaire meet the standards. The Cronbach's α coefficient of the total questionnaire is 0.967, and the split-half reliability is 0.967, indicating good reliability of the questionnaire. Exploratory factor analysis extracted a total of 3 common factors, with a cumulative variance contribution rate of 61.892%. Confirmatory factor analysis shows that all the main evaluation indicators are within the acceptable range of the judgment criteria (factor loadings range from 0.626 to 0.729, χ2/df=1.840, CFI=0.765, TLI=0.896, RMSEA=0.062, SRMR=0.064). The I-CVI of the questionnaire is 0.87 to 1.00, and S-CVI/Ave=0.968. Conclusions The questionnaire for the prevention and control of respiratory infectious diseases among community residents formed in this study has good reliability and validity and can be used to assess the literacy level of community residents in the prevention and treatment of respiratory infectious diseases.
  • The impact of social support on the self-advocacy ability of breast cancer patients
    HUANG Jiaqian, CHEN Hongtao, HE Susu, XIAO Shanshan, LI Wen, DENG Peng, SONG Xiaohua
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 192-197.
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    Objective It aims to explore the impact of social support on the self-advocacy ability of breast cancer patients, and to provide a strong basis for the development of personalized support plans and the enhancement of patients' autonomous decision-making ability. Methods A general information questionnaire, the Female Cancer Self-Advocacy Scale, the Comprehending Social Support Scale, and the Self-Identity Scale were used to investigate 364 breast cancer patients in the Breast Oncology Department of a tertiary hospital in Hunan Province. Results Self-advocacy and comprehension social support scores of 364 breast cancer patients were (77.25±8.99) and (62.92±8.32), respectively, and Pearson's correlation analysis showed that self-advocacy was positively correlated with comprehension social support (r=0.602, P<0.01). Informed decision-making dimension, effective communication dimension, and effective social support dimension were positively correlated with comprehending social support (r values of 0.605, 0.442, and 0.300, respectively, all P<0.01), and the analysis results showed that age, residence status, knowledge of breast cancer-related knowledge, whether or not to functionally exercise, and comprehending social support were the influencing factors of patients' ability to self-advocacy. Social support was an independent influence on patient self-advocacy, explaining 45% of the total variance. Conclusion The self-advocacy ability of breast cancer patients is at an intermediate to high level, and self-advocacy ability is closely related to social support; future research and clinical practice should focus on enhancing the social support system of breast cancer patients to promote the development of their self-advocacy ability. By strengthening social support, healthcare providers can effectively optimize treatment outcomes and improve the overall psychological health of patients, which has important clinical significance and potential application value.
  • A study on the correlation between depressive symptoms and lifestyles of rural elderly in Hunan province
    LIN Xue, HUANG Lifang, CHEN Zhou, LIU Yufei, GONG Fanghua
    Journal of Hunan Normal University(Medical Science). 2025, 22(3): 198-204.
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    Objective To investigate the prevalence of depressive symptoms and their influencing factors among the rural elderly population in Hunan province. Methods Stratified random sampling method was adopted to survey rural elderly people in 10 townships in 5 regions of Hunan Province from May to June 2024, and they were investigated by general information questionnaire, GDS-15 Geriatric Depression Scale, PARS-3 Physical Activity Rating Scale, ISI-7 Insomnia Index Scale, and DDS-9 Dietary Diversity Scale, and analyzed by using the χ2 test, Mann-Whitney U test, and the multifactorial bicategory Logistic regression analysis model to analyze the influencing factors of depressive symptoms in the elderly. Results A total of 6 790 questionnaires were distributed in this study, and 6 305 were returned as valid, with a depression symptom detection rate of 30.75% . Logistic regression results showed that living alone (OR=1.448, 95% CI: 1.176-1.782), not being in a marriage (OR=1.347, 95% CI: 1.161-1.564), and not using the Internet (OR=1.413, 95% CI: 1.219-1.638), smoking (OR=1.360, 95% CI: 1.148-1.610), mild insomnia (OR=2.925, 95% CI: 2.559-3.344), and moderate insomnia (OR=2.350, 95% CI: 1.705-3.238) were the risk for geriatric depression Factors. Financial subsidies from children (OR=0.690, 95% CI: 0.589-0.809), low physical activity (OR=0.553, 95% CI: 0.463-0.613), moderate physical activity (OR=0.476, 95% CI: 0.402-0.563), high physical activity (OR=0.334, 95% CI: 0.274-0.407), and dietary intake (OR=0.334, 95% CI: 0.274-0.407) were the risk factors for depression in older adults.0.407), and dietary diversity (OR=0.906, 95% CI: 0.873-0.940) were protective factors for depression in the elderly. Conclusion The prevalence of depressive symptoms is high among rural older adults in Hunan Province. Rural mental health management and services should be strengthened to effectively prevent and reduce the occurrence of geriatric depression.
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