Clinical significance of USP1 expression in ovarian cancer and its effect on malignant phenotype of ovarian cancer cells

YU Bo; PENG Peng; HUANG Liang; BAO Liwei; WANG Zihan; WANG Weimin; ZHANG Xia

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Journal of Hunan Normal University(Medical Science) ›› 2024, Vol. 21 ›› Issue (2) : 39-44.

Clinical significance of USP1 expression in ovarian cancer and its effect on malignant phenotype of ovarian cancer cells

YU Bo^1,2, PENG Peng¹, HUANG Liang¹, BAO Liwei¹, WANG Zihan¹, WANG Weimin¹, ZHANG Xia¹

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Abstract

Objective To investigate the expression of ubiquitin-specific peptidase 1(USP1) in ovarian cancer and its correlation with clinicopathologic features of patients, as well as to explore the impact of USP1 on the malignant phenotype of ovarian cancer cells. Methods The expression level of USP1 in ovarian cancer patients and its relationship with clinical stage were analyzed by bioinformatics. Immunohistochemistry was used to detect the expression of USP1 in ovarian cancer patients and normal ovarian tissues, and to analyze the relationship between its expression level and the clinical characteristics of the patients. The SKOV3 cell line overexpressing USP1 was constructed by lentiviral infection, and the cell proliferation and clone formation abilities were detected by CCK-8 and clone formation assay, while the migration and invasion abilities were detected by Transwell assay. Results The expression level of USP1 in ovarian cancer tissues was significantly higher than that in normal ovarian tissues, and was correlated with the pathological type, peritoneal metastasis and the size or invasion degree of tumor in situ, but was not correlated with the expression levels of Ki67, ER, PR and P53. The results of in vitro experiments showed that USP1 promoted the proliferation, colony formation, migration and invasion ability of ovarian cancer cells. Conclusion USP1 expression was enhanced in ovarian cancer tissues and correlated with patients＇ pathological type, peritoneal metastasis, and clinical stage, and USP1 promoted the progression of the malignant phenotype of ovarian cancer cells.

Key words

ubiquitin specific peptidase 1(USP1) / ovarian cancer / cell proliferation / migration

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YU Bo, PENG Peng, HUANG Liang, BAO Liwei, WANG Zihan, WANG Weimin, ZHANG Xia. Clinical significance of USP1 expression in ovarian cancer and its effect on malignant phenotype of ovarian cancer cells[J]. Journal of Hunan Normal University(Medical Science). 2024, 21(2): 39-44

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