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Quick Search     Advanced Search   2023 Vol.  20 No.  1 Published: 2023-02-25 Basic Medicine Clinical Medicine Preventive Medicine Nursing Science Basic Medicine 1 SUN Fei, YUAN Qing, ZHANG Jiansong Apigenin Inhibits Glycolysis and Spheroid Formation via Downregulation of HK-2 Expression in Non-Small Cell Lung Cancer H460 Cells Objective To investigate whether apigenin (API) inhibits the glycolysis and self-renewal capacity by downregulating the expression of glucose metabolism related kinase HK-2 in non-small cell lung cancer (NSCLC) H460 cells in vitro. Methods H460 cells were transfected with HK-2 cDNA, treated with sub-cytotoxicity concentrations of API (20 μM; 40 μM and 80 μM) or in combination with API (80 μM) treatment and HK-2 cDNA transfection. Western blot was utilized to detect the expression of HK-2. The levels of lactate production and glucose consumption were using measurement of lactate production and glucose consumption assays. The spheroid formation assay was employed to detect the self-renewal capacity in H460 cells. Results The sub-cytotoxic concentrations of API decreased the expression of glucose metabolism related kinase HK-2 and reduced lactiate production and glucose consumption, as well as decreased sphere formation rate in NSCLC H460 cells in a dose-dependent tendency. Moreover, overexpression of HK-2 could rescue the inhibitory effects of API on lactate production, glucose consumption and spheroid formation in NSCLC H460 cells. Conclusion API can inhibit glycolysis and self-renewal capacity by downregulating HK-2 expression in NSCLC H460 cells. 2023 Vol. 20 (1): 1-5 [Abstract] ( 505 ) [HTML 1KB] [PDF 3706KB] ( 2370 ) 6 LUO Yutao, SU Qing, LI Rui, YANG Rui, ZHENG Tongqi, WEI Yunfu, SUN Guoying Spermidine reduces LPS-induced ALI by inhibiting the NF-κB/ NLRP3 inflammasome pathway Objective The purpose of this study was to observe the effect of spermidine on the expression and activation of NLRP3 in lipopolysaccharide (LPS) - induced acute lung injury (ALI) mice. Methods Effectof spermidine on respiratory function in ALI mice was detected by a small animal ventilator, and the survival rate of mice was detected. Pathological score were used to evaluate the effect of spermidine on the morphological changes of lung tissue in ALI mice, and levels of IL-1β and IL-18 protein levels in bronchoalveolar lavage fluid (BALF) of ALI mice were detectd by ELISA. The qPCR was used to detect the expression of IL-1β, IL-18, NLRP3, ASC, pro-caspase-1 and NF-κB in mouse lungs; IκB expression of mice was detected by Western blots. Results Spermidine could improve the respiratory function and survival rate of ALI mice, alleviate LPS induced lung pathological damage, and reduce IL-1β, IL-18, NLRP3, ASC, pro-caspase-1, NF-κB and IκB expression of ALI mice. Conclusions Spermidine may reduce the assembly and activation of NLRP3 inflammasome by inhibiting the activation of NF-κB in the lung tissue of ALI mice, thereby inhibit the expression of inflammatory factors, and ultimately lessening ALI. This study can provide an experimental basis for exploring the mechanism of ALI from the perspective of regulating endogenous energy metabolism. 2023 Vol. 20 (1): 6-12 [Abstract] ( 624 ) [HTML 1KB] [PDF 2926KB] ( 2091 ) 13 LIAO Tianlong, CHU Xuetong, YANG Xiaoping Mechanism of Icariin I promoting proliferation of mouse spermatogenic stem cells Objective The medicine icariin I is applied to mouse spermatogonial stem cells to explore the effect of the medicine on the proliferation function of the cells and the related mechanism for the effect. Methods The changes in the value-added ability of mouse spermatogenic stem cells in the presence of icariin I were detected by using the MTT and EdU cell proliferation methods. The changes of Bmi1, p-Akt, and Akt proteins in mouse spermatogenic stem cells in the presence of icariin I were detected by Western Blot experiment. The expression of related genes was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Finally, inhibitors and siRNA were used to explore regulatory relationship between p-Akt and Bmi1 protein. Results Icariin I at the concentration of 0.5-1µM could significantly enhance the proliferation of mouse spermatogenic stem cells as compared with that in the control group (P<0.05). Meanwhile, within the same concentration range, the expression levels of p-Akt and Bmi1 proteins in mouse spermatogenic stem cells were significantly increased under the action of drugs (P<0.05). P-Akt and Bmi1 protein levels were significantly down-regulated in the AKT inhibitor group, and the difference was statistically significant with the control group (P<0.05). There were no significant changes in Akt mRNA levels in the siBmi1 group compared with the control group (P>0.05). Conclusion Icariin I can enhance the proliferation of mouse spermatogonial stem cells, specifically by activating Akt signaling pathway to up-regulate the expression of Bmi1. 2023 Vol. 20 (1): 13-17 [Abstract] ( 544 ) [HTML 0KB] [PDF 3277KB] ( 2575 ) 18 SHI Rong, MO Hanlin, LI Yangbo, ZHANG Zhibai, LI Hao Role and mechanism of galactoglutinin-1 in metformin inhibition of proliferation of cholangiocarcinoma cells Objective To investigate the role and molecular mechanism of galactose lectin-1(Galectin-1, Gal-1) in the inhibition of cholangiocarcinoma cell proliferation by metformin. Methods HuCCT1 cells were treated with different doses of metformin for 24, 48 and 72 h. The effect of metformin on cell survival was detected by CCK8, and cells were treated with 0 and 20 mmol /L of metformin for 24 h. The effects of metformin on cell clone formation, cell cycle and apoptotic vesicles were analyzed by clone formation assay, flow cytometry and DAPI staining. The expression of Gal-1 in cholangiocarcinoma and its effect on the survival rate of cholangiocarcinoma patients were analyzed using TCGA, GEPIA, UALCAN, HPA and other databases; the expression changes of Gal-1 in intrahepatic cholangiocarcinoma tissues and paracancerous tissues were detected by immunohistochemistry; cells were treated with 0 and 20 mmol /L metformin for 24 h, and Gal-1 and the protein PI3K, which is closely related to cell proliferation, were detected using Western Blot. The cells were treated with 0 and 20 mmol/L metformin for 24 h. The protein expression of Gal-1 and closely related proteins PI3K, AKT and mTOR were detected by Western Blot. Results The cell survival rate decreased significantly with the increase of metformin treatment concentration and treatment time, and the results of clone formation assay showed that the clone formation rates of control and 20 mmol/L metformin treated groups were 18.17% and 1.98%, respectively. The metformin treated groups showed significant G2 phase cell block and were accompanied by the formation of a large number of apoptotic vesicles. The results of biochemical analysis showed that Gal-1 expression was significantly increased in cholangiocarcinoma cells, and the results of immunohistochemistry using cancer and paraneoplastic tissues were consistent with the results of biochemical analysis, and the high expression of Gal-1 was closely associated with OS in cholangiocarcinoma patients; the expression of Gal-1 was significantly decreased in metformin-treated HuCCT1 cells, and accompanied by the inhibition of PI3K/AKT signaling pathway; in addition , inhibition of Gal-1 expression not only reduced cell proliferation ability but also restricted the inhibition of PI3K/AKT signaling pathway. Conclusion Metformin can inhibit the activation of PI3K/AKT signaling pathway by suppressing Gal-1 expression in intrahepatic cholangiocarcinoma, which in turn leads to the inhibition of cell proliferation. 2023 Vol. 20 (1): 18-23 [Abstract] ( 450 ) [HTML 0KB] [PDF 2802KB] ( 1017 ) 24 LUO Yanji, HUANG Zhihong, XIAO Chuangqing, YE Chunmei, ZHONG Jie, CAO Wenjing Study on the correlation between the reduction of islet β-cell function and the ratio of GLP-1/GLU in severely scalded MODS mice Objective To study the correlation between the reduction of islet β-cell function and the ratio of glucagon-like peptide 1/glucose (GLP-1/GLU) and explore its mechanism in severely scalded MODS mice so as to provide experimental basis for the prevention and treatment of the reduction of islet β-cell function in severely scalded MODS from a new perspective of improving the function of GLP-1 secretion in intestine. Methods 90 Kunming mice of Specific Pathogen Free (SPF) grade were randomly divided into three groups: 10 mice as the control group, 30 mice as simple scald group and 50 mice as MODS group. The mice in MODS group were made into MODS mice with 30% total body surface area Ⅲ degree scald and intraperitoneal injection of LPS. The mice in simple scald group were only inflicted with 30% total body surface area Ⅲ degree scald, and mice in the control group were sham scalded. Blood samples of mice in the control group were collected. At 1 day and 3 days after injury, 10 mice were randomly captured respectively from simple scald group and MODS group, and blood samples were collected. At 5 days after injury, blood samples of all surviving mice in scald group and MODS group were collected. The levels of GLU, plasma insulin, GLP-1 and diamine oxidase (DAO) of mice were determined. Homeostasis model assessment of β-cell function (HOMA-β) was applied, and then the index of islet β-cell function (HOMA-β) and the ratio of plasma GLP-1/GLU were calculated and observed. The correlation coefficient between HOMA-β and plasma GLP-1/GLU was calculated. The ileum tissues of mice were collected to observe the expression of GLP-1 by immunohistochemistry, and then the levels of intestinal GLP-1 secretion relative to blood glucose (intestinal GLP-1/GLU) were calculated and observed. The correlation coefficient between plasma GLP-1/GLU and intestinal GLP-1/GLU, and the correlation coefficient between intestinal GLP-1/GLU and plasma DAO were calculated. Data were statistically analyzed with one-way analysis of variance and Bonferroni test, Kruskal-Wallis and Bonferroni test, and Spearman rank correlation analysis. Results HOMA-β, plasma GLP-1/GLU and intestinal GLP-1/GLU of severely scalded MODS mice at 1, 3 and 5 days after injury were significantly lower than those of mice in the control group and simple scald group at the same time point (P<0.05). HOMA-β was positively correlated with plasma GLP-1/GLU of mice (r=0.685, P<0.001). Plasma GLP-1/GLU was positively correlated with intestinal GLP-1/GLU (r=0.851, P<0.001). Intestinal GLP-1/GLU was negatively correlated with DAO (r=-0.732, P<0.001). Conclusion The islet β-cell function and the ratio of GLP-1/GLU were decreased in severely scalded MODS mice. Insufficiency of plasma GLP-1 relative to blood glucose was correlated with the reduction of islet β-cell function. Intestinal damage led to relatively insufficient GLP-1 secretion. Protecting intestinal cells to increase the ratio of plasma GLP-1/GLU in severely scalded MODS mice can be used as an important measure to improve the reduction of islet β-cell function. 2023 Vol. 20 (1): 24-30 [Abstract] ( 520 ) [HTML 0KB] [PDF 3521KB] ( 1314 ) Clinical Medicine 31 KANG Jiawen, XIANG Xiaoqing, JIANG Jingwen, GUO Sihui, ZHANG Yong, LI Lesai Differential gene expression and immune infiltration landscape in high-grade neuroendocrine carcinoma of cervix Objective To explore gene expression characteristics and immune infiltration status of high-grade neuroendocrine cervical carcinoma (HGNECC). Methods We integrated the sequencing data of GTEx, TCGA, and GEO databases about patients with normal cervix and HGNECC. Then we searched for differential genes (DEGs) between normal and tumor samples, and GO and KEGG analysis were used to explore the enrichment of differential gene-related pathways. Then immune-related genes (IRGs) and DEGs were identified in IMMPORT website to find immune-related differential genes by VENN plots, and then core genes (Hubs) were identified by protein interaction network (PPI) and MCODE and cytohubba plugins in cytoscape software. Next, we performed the analysis of difference in immune infiltration between normal cervical tissue and HGENCC, and the ssGSEA algorithm was used to assess the immune infiltration of the microenvironment in HGNECC and to compare the expression of different immune checkpoint genes within patients with different immune activity. Finally, we predicted the drug-target interaction network in DGIdb website, expecting to explore potential small molecule targeted drugs with efficacy in HGNECC. Results Through analyzing expression of DEGs between normal and tumor samples, ten Hub genes including CXCL8, IL1B, CSF2, CCL4, IL10, CCL3, CCL3L3, PTPN11, CBL and SOS1 were identified in HGNECC. In the immune infiltration analysis by CIBERSORT algorithm and ssGSEA algorithm, we found a significant change in mast cells from quiescent to activated state in patients with high-grade neuroendocrine tumors of the cervix, and then we hypothesized that the mast cells of activation status were increased in HGNECC patients; Finally, based on the predicted Hub genes, the drug selection for HGNECC patients was recommended for ABX-IL8, RILONACEPT and other drugs. Conclusion This study provides new ideas for the clinical treatment of HGNECC by bioinformatics analysis based on public data. 2023 Vol. 20 (1): 31-38 [Abstract] ( 472 ) [HTML 0KB] [PDF 7589KB] ( 666 ) 39 HUANG Bo, YUAN Yuanyuan, ZHANG Guanying To explore the related factors and clinical significance of atherosclerosis cardiovascular disease in postmenopausal breast cancer women treated with endocrine therapy by combining proteomics and metabolomics Objective To analyze the risk factors of atherosclerotic cardiovascular disease (ASCVD) in postmenopausal breast cancer (BC) women receiving endocrine therapy and its clinical predictive significance based on proteomics and metabolomics multi-platform omics association. Methods A total of 147 patients with invasive breast cancer admitted to our hospital from January 2020 to September 2021 were selected as the research objects. According to carotid IMT examination and clinical comprehensive diagnosis, the patients were divided into ASCVD group (68 cases) and non-ASCVD group (79 cases). The clinical data of the two groups were collected, and the lipid molecules related to ASCVD were analyzed by multi-platform proteomics and metabolomics association analysis. The factors with P<0.05 in univariate analysis were included and multivariate Logistic analysis was used to analyze the risk factors of ASCVD in postmenopausal breast cancer patients with endocrine therapy. Receiver operating characteristic (ROC) curve was drawn to analyze the diagnostic value of risk factors for ASCVD in breast cancer patients. Results Triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) were identified as lipoprotein molecules significantly associated with ASCVD in breast cancer women by multi-platform omics analysis. Univariate analysis showed that there were significant differences in HDL-C, TC, LDL-C, TG, hypertension, and non-steroidal AI drugs between the ASCVD group and the non-ASCVD group. Multivariate Logistic analysis showed that the use of non-steroidal AI drugs, HDL-C, TC, LDL-C and TG were risk factors for ASCVD in postmenopausal breast cancer patients. The AUC of HDL-C, TC, LDL-C, TG, hypertension, and non-steroidal AI drugs in the diagnosis of ASCVD in postmenopausal breast cancer treated with endocrine therapy were all >0.73, and the sensitivity and specificity were all >60.00%. Conclusions The use of non-steroidal AI drugs, HDL-C, TC, LDL-C and TG are the main risk factors for ASCVD in postmenopausal breast cancer women with endocrine therapy, which have important guiding significance in predicting ASCVD in such patients. 2023 Vol. 20 (1): 39-43 [Abstract] ( 454 ) [HTML 0KB] [PDF 2212KB] ( 1505 ) 44 WEI Yan, LI Fang, MA Zhuangzhuang, ZHAO Jing, ZHANG Haiyan, LI Yanli Detection of Serum MBL, MASP1 and MASP2 in Adult Acute Leukemia and Its Clinical Value Objective To Detect the serum levels of MBL, MASP1 and MASP2 in adults with acute leukemia and study their clinical value Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of serum MBL, MASP1 and MASP2 in adult acute leukemia patients and normal healthy people, and receiver operating characteristic curve was used to analyze the sensitivity, specificity and accuracy of serum MBL, MASP1 and MASP2 in the diagnosis of adult acute leukemia. Results There was no significant difference in MBL between the leukemia group and the control group. The serum MASP1 level of the leukemia group was significantly lower than that of the control group, while the serum MASP2 level was significantly higher than that of the control group. People. The serum MBL level in patients with relapsed leukemia was significantly lower than that in patients with first-onset leukemia, and the level of MASP2 was significantly higher than that in patients with first-onset leukemia, while there was no significant difference in serum MASP1 between the two groups. Logistic regression analysis showed that serum levels of MBL, MASP1, MASP2 were associated with adult leukemia. The sensitivity, specificity and accuracy of MBL, MASP1 combined with MASP2 in the diagnosis of adult acute leukemia were 0.854, 0.810 and 0.664, respectively. Conclusion Serum MASP1 was significantly decreased while MASP2 was significantly increased in adult leukemia patients. The combined detection of MBL, MASP1 and MASP2 has a high diagnostic value for adult leukemia. 2023 Vol. 20 (1): 44-47 [Abstract] ( 494 ) [HTML 1KB] [PDF 1973KB] ( 2029 ) 48 LIAO Zhaomei, CAI Siming, CHEN Jianbiao, LV Hualong, LEI Zhixian Pathogenic bacteria characteristics and Clinical significance of immunoglobulin, CRP, WBC and PCT in children with refractory pneumonia Objective To investigate the etiological characteristics of infection and the expression significance of immunoglobulin, C-reactive protein (CRP), white blood cell count (WBC) and procalcitonin (PCT) in children with refractory pneumonia (RMPP). Methods The clinical data of 213 children with RMPP and 87 children with general pneumonia (GMPP) admitted from January 2019 to March 2021 were retrospectively analyzed. Peripheral blood of two groups of children was collected to detect the levels of immunoglobulin, CRP, WBC, and PCT, bronchoalveolar lavage fluid (BALF) of RMPP children was collected for pathogen culture and drug sensitivity test. The receiver operating characteristic (ROC) curve was used to analyze the value of immunoglobulin, CRP, WBC, and PCT in the differential diagnosis of RMPP. Results A total of 158 strains of pathogens were detected in 13 children with RMPP, including 98 strains of Gram-negative bacteria and 60 strains of Gram-positive bacteria. The Gram-negative bacteria were mainly Klebsiella pneumoniae (29 strains), while the Gram-positive bacteria were mainly Streptococcus pneumoniae (31 strains) and Staphylococcus aureus (24 strains). The results showed that Klebsiella pneumoniae was sensitive to imipenem, ertapenem, piperacillin/tazobactam, tobramycin and amikacin. Streptococcus pneumoniae was sensitive to ceftriaxone, cefotaxime, meropenem, ertabenem, vancomycin, Linezolid, chloramphenicol, moxifloxacin, levofloxacin, while Staphylococcus aureus was sensitive to gentamicin, vancomycin, quinuprtin/Dafoputine, linezolid, tegacycline, moxifloxacin, and rifampicin. There was no difference in the levels of IgA and WBC between the two groups; The levels of IgG, IgM, CRP, and PCT in the RMPP group were higher than those in the GMPP group. The ROC curve shows that IgG, IgM, CRP, and PCT have a certain differential diagnosis value for RMPP (AUC=0.770, 0.778, 0.824, 0.806, P<0.001), and the combination of the four (AUC=0.902, P<0.001) has a differential diagnosis value Higher. Conclusion The pathogenic bacterias of children with RMPP are mainly Klebsiella pneumoniae, Streptococcus pneumoniae and Staphylococcus aureus. The above pathogenic bacterias should be covered during empirical treatment, and the pathogen should be detected as soon as possible. If the curative effect is not good, drug resistance should be considered . And promptly give targeted treatment based on the results of drug sensitivity. The IgG, IgM, CRP and PCT level apparently higher than GMPP group, and RMPP will not have a significant impact on WBC. Combined detection of IgG, IgM, CRP, and PCT can be used for the differential diagnosis of RMPP. 2023 Vol. 20 (1): 48-51 [Abstract] ( 448 ) [HTML 1KB] [PDF 2248KB] ( 1051 ) 52 YANG Wei, XU Ming, WANG De'en, ZHANG Xiangcheng Bedside Transthoracic Echocardiography in Evaluation of Left Ventricular Function in Patients with Fulminant Myocarditis and Myocardial Infarction Undergoing VA-ECMO Support Objective To evaluate the left ventricular systolic function of patients with fulminant myocarditis and myocardial infarction during veno-arterial extracorporeal membrane oxygenation (VA-ECMO) therapy using bedside transthoracic echocardiography, so as to explore the application value of bedside echocardiography during VA-ECMO therapy. Methods A retrospective analysis was carried out on 32 patients undergoing VA-ECMO support in our hospital. Sixteen patients with cardiogenic shock and successful weaning were selected and divided into two groups: the fulminant myocarditis group (n=8) and the myocardial infarction group (n=8). Further inter-group and intra-group comparisons were performed on the general clinical data, left atrial diameter (LAD), left ventricular end-diastolic diameter (LVDD), and left ventricular ejection fraction (LVEF) before VA-ECMO support, 24 hours after VA-ECMO support, and after the successful weaning, respectively. Results (1) Comparison of general clinical data: The average age of the fulminant myocarditis group was smaller than that of the myocardial infarction group [(34.2±9.0) vs. (64.1±6.9) years]. (2) Comparison within groups: The average level of LVDD before VA-ECMO support establishment in explosive myocarditis group was lower than that after 24h (46.7±8.8) mm. The average level of LVEF after successful weaning was higher than that 24 hours after VA-ECMO support [ (51.5±21.3) % vs. (33.9±18.4) %]. Furthermore, in the myocardial infarction group, there were no significant differences in LAD, LVDD and LVEF before VA-ECMO support was established, 24h after VA-ECMO support was established, and after successful withdrawal. (3) Comparison between the two groups: The LAD in explosive myocarditis group was lower than that in acute myocardial infarction group before VA-ECMO support was established, 24h after VA-ECMO support was established and after successful withdrawal. The average LVDD level of the myocardial infarction group was higher than that of the fulminant myocarditis group before VA-ECMO support. Besides, The LVEF of explosive myocarditis group was higher than that of acute myocardial infarction group. Conclusions VA-ECMO support can significantly improve LVEF in patients with myocarditis and the impact of fulminant myocarditis on cardiac function is possibly reversible. VA-ECMO support did not significantly improve LAD, LVDD and LVEF in myocardial infarction group, but could buy a period of time for other purposes of treatment. LAD in explosive myocarditis group was lower than that in acute myocardial infarction group before VA-ECMO support was established, 24h after VA-ECMO support was established, and before ICU transfer after successful withdrawal . Age, LAD and LVDD may affect the recovery of cardiac function. 2023 Vol. 20 (1): 52-56 [Abstract] ( 495 ) [HTML 0KB] [PDF 2203KB] ( 1381 ) 57 WU Pengyu, LIU Jun, SHAN Jing, LIU Li, PU Wenjing, XUE Mei, SUN Xiaobin, WANG Qiong Comparison of effects of early fluid resuscitation at different rates on hemodynamics and prognosis of initially assessed non-severe acute pancreatitis Objective To investigate the application effects of different rates of early fluid resuscitation on patients with non-severe acute pancreatitis (NSAP) at initial assessment. Methods The clinical data of 336 patients with NSAP at initial assessment were retrospectively analyzed. According to the early fluid infusion rate, the patients were divided into high-rate fluid infusion group (191 cases, total fluid resuscitation at 24 h >1/2 of total fluid resuscitation at 48 h after admission) and low-rate fluid infusion group (145 cases, total fluid resuscitation at 24 h ≤1/2 of total fluid resuscitation at 48 h after admission). The total fluid infusion at 24 h, 48 h and 72 h after admission was recorded in the two groups, and the changes in peripheral blood biochemical indicators [C-reactive protein (CRP), hematocrit (HCT), serum creatinine (Cre) ] were compared at 12 h, 48 h, 72 h, and 7 d after admission. The prognosis were analyzed in the two groups of patients. Results The total fluid infusion at 24 h and 48 h after admission in high-rate fluid infusion group was significantly higher than that in low-rate fluid infusion group (P<0.05), and the CRP and HCT levels at 12 h after admission and CRP level at 72 h after admission, time of vital signs reaching the standard, incidence rate of systemic inflammatory response syndrome (SIRS), incidence rate of multiple organ dysfunction syndrome (MODS), rate of invasive (interventional) operation, mechanical ventilation rate, rate of intensive care treatment and rate of conversion to severe condition were significantly lower than those in low-rate fluid infusion group (P<0.05). Conclusion High-rate fluid infusion is beneficial to improving the hemodynamics and prognosis of patients with NSAP at initial assessment, with high clinical application value. 2023 Vol. 20 (1): 57-60 [Abstract] ( 488 ) [HTML 0KB] [PDF 1776KB] ( 1128 ) 61 MAO Wanfu, HE Jun, SU Xiaotao, LIU Wei Clinical studies of percutaneous pedicle screws in the treatment of thoracolumbar fractures: unilateral, bilateral screws and screw-free in the injured vertebrae Objective To evaluate the effect of percutaneous pedicle screw fixation (PPSF) using three different treatments on thoracolumbar fractures. Methods A total of 63 patients with single-segment thoracolumbar fractures treated with PPSF, without neurological symptoms, and with a Load sharing classification (LSC) score <7 were selected. The patients were divided into Group A (transvertebral fixation), Group B (unilateral pedicle screw fixation), and Group C (bilateral pedicle screw fixation). The clinic data of operative time, bleeding, Visual analog score (VAS), and Oswestry disability index (ODI) were assessed in the three groups. The changes of the Anterior height ratio of injured vertebral (AHRIV) and the Cobb angle of injured vertebral (CAIV) in the sagittal plane were also assessed using imaging techniques. Results There was no statistically significant difference between the three groups in terms of VAS scores at each postoperative follow-up and ODI scores at the last follow-up. In terms of operative time, the differences between the three groups were statistically significant when compared with each other. In terms of bleeding volume, the differences were statistically significant in group C compared with groups A and B, respectively. In terms of AHRIV, there was a statistically significant difference between group A and group C at the last postoperative follow-up, and there was a statistically significant difference between group A compared with its own first postoperative follow-up. In terms of postoperative CAIV loss, there was a statistically significant difference between the two follow-ups in group A, and the difference was statistically significant in group A compared with group B and with group C. Conclusion The use of PPSF for single-segment thoracolumbar fracture with TLSC <7 has achieved good results by performing Unilateral pedicle screw fixation, and can effectively reduce loss of AHRIV and CAIV in the middle and distant stages of repositioning, which can achieve more satisfactory clinical results. 2023 Vol. 20 (1): 61-67 [Abstract] ( 476 ) [HTML 1KB] [PDF 2058KB] ( 2010 ) 68 FENG Dongqian, ZHANG Bin, YU Jiasheng, BAO Lei, XIN Bing Comparison of cement perfusion combined with PFNA internal fixation and PFNA alone in the treatment of osteoporotic intertrochanteric fractures in the elderly Objective To compare the efficacy of bone cement perfusion combined with proximal femoral nail anti-rotation (PFNA) internal fixation and PFNA alone in the treatment of osteoporotic intertrochanteric fractures in the elderly. Methods A total of 96 elderly patients with osteoporotic intertrochanteric fracture admitted to our hospital from January 2019 to December 2021 were included in the study and divided into observation group and control group by random number table method, with 48 cases in each group. The control group was treated with PFNA internal fixation, while the observation group was treated with bone cement perfusion combined with PFNA internal fixation. Recorded and compared the perioperative indicators of the two groups, evaluated the hip function of the patients at 1 month, 3 months and 6 months after operation through Harris Hip scale, evaluated the pain degree of the patients before operation, 1 month, 3 months and 6 months after operation through Visual analogue scale (VAS), recorded postoperative complications in the two groups, and calculated the total incidence of complications. Results The intraoperative blood loss, length of hospital stay and postoperative weight-bearing time in the observation group were significantly lower than those in the control group, and the operation time in the observation group was significantly longer than that in the control group. There was no significant difference in postoperative blood loss between the two groups. Compared with 1 month after operation, the Harris score of the observation group and the control group was significantly increased at 3 months and 6 months after operation, and the Harris score of the observation group was significantly higher than that of the control group at 1 month, 3 months and 6 months after operation. The VAS scores of the observation group and the control group at 1 month, 3 months and 6 months after operation were significantly decreased compared with those before operation, and the VAS scores of the observation group at 1 month, 3 months and 6 months after operation were significantly lower than those of the control group. There was no significant difference in the total incidence of complications between the two groups. Conclusion Bone cement perfusion combined PFNA internal fixation in the treatment of osteoporotic elderly femoral intertrochanteric fracture curative effect is better than that of pure PFNA treatment, patients can significantly reduce intraoperative blood loss, shorten the patients' hospitalization duration, down load time, at the same time can effectively improve the function of patients with hip, relieve patients' pain, and has high security, have clinical application value. 2023 Vol. 20 (1): 68-71 [Abstract] ( 494 ) [HTML 0KB] [PDF 1918KB] ( 1394 ) 72 SUN Xudu, DONG Mei, ZHANG Shan Effect of dagglitazone combined with amiodarone in the treatment of type 2 diabetes mellitus complicated with paroxysmal atrial fibrillation and its effect on left atrial function and left atrial appendage volume Objective To observe the efficacy of Dapagliflozin combined with amiodarone in the treatment of patients with type 2 diabetes mellitus (T2DM) complicated with paroxysmal atrial fibrillation (PAF) and its effect on left atrial function and left atrial appendage volume. Methods 94 patients with T2DM complicated with PAF admitted to the Department of Cardiology of the third Xingtai hospital from August 2020 to November 2021 were selected as the research objects. They were randomly divided into control group and observation group, 47 cases in each group. The patients in the control group were treated with amiodarone orally after the treatment of basic diseases, and the patients in the observation group were treated with Dapagliflozin orally on the basis of the treatment of the control group. Both groups were treated for 6 months. The frequency of PAF, duration and average heart rate were observed before treatment, 3 months after treatment and 6 months after treatment; Doppler ultrasound was used to examine the changes of left atrial anteroposterior diameter (LAD), left atrial end diastolic area (LAEDA), atrial volume (AV), left atrial ejection fraction (LAEF) and left atrial appendage end diastolic volume (LAAEDV). Results The results of repeated measurement of variance showed that there were significant differences between the observation group and the control group in PAF attack frequency, duration, mean heart rate, FPG, 2hPG, HbA1c, LAD, LAEDA, AV, LAEF and LAAEDV at groups and time points before treatment, 3 months after treatment and 6 months after treatment; Compared with the control group, the number of PAF episodes, PAF duration, mean heart rate, FPG, 2hPG, HbA1c, LAD, LAEDA, AV and LAAEDV decreased, and LAEF increased in the observation group after treatment; The clinical efficacy of the observation group was better than that of the control group, and there was no significant difference in the incidence of adverse reactions between the two groups. Conclusion Dapagliflozin combined with amiodarone has a good clinical effect in the treatment of T2DM complicated with PAF. The mechanism may be related to the further improvement of glucose metabolism and the improvement of left atrial structure and function. 2023 Vol. 20 (1): 72-75 [Abstract] ( 576 ) [HTML 0KB] [PDF 2192KB] ( 1515 ) 76 CAO Yang, LIU Fang Volume-effect relationship between total blood loss and serum biochemical index changes after total hip arthroplasty Objective To investigate the pattern of changes in serum potassium, calcium, albumin (ALB) and hemoglobin (HGB) after traumatic total hip replacement (THA), so as to provide a basis for perioperative safety management. Methods The data of 112 patients who underwent THA for femoral neck fracture at the Department of Arthroplasty of Yueyang People's Hospital from September 2017 to September 2022 were retrospectively analyzed. Results The mean total blood loss after THA was (798.74±265.34) mL, and the hidden blood loss was (428.12±195.45) mL, accounting for 54.21% of the total blood loss; serum calcium, ALB, and HGB levels decreased significantly after total hip replacement compared with preoperative levels, while serum potassium levels did not change significantly compared with preoperative levels; total THA blood loss was significantly and positively correlated with the amount of change in patients’ serum calcium, ALB and HGB, and the regression equations were Y=0.000216X, Y=0.00919X, Y=0.0280X, with R2: 0.738, 0.860, 0.945, respectively; The incidence of postoperative biochemical index abnormalities was significantly increased in patients with preoperative serum calcium <2.27mmol/L, ALB<37.26g/L, HGB<122.11g/L. Conclusion There is a quantitative relationship between the decrease in serum calcium, ALB, and HGB concentrations and the total blood loss in THA. In clinical practice, the change values of postoperative blood biochemical indexes can be predicted based on the mean blood loss in the general population, so as to set up the preoperative warning value of relevant indicators, which provides effective help to estimate the changes of corresponding indicators after THA and advance intervention. 2023 Vol. 20 (1): 76-79 [Abstract] ( 478 ) [HTML 0KB] [PDF 2328KB] ( 1150 ) 80 YANG Hongxiang, YAN Hongmei, WEN Junjie Correlation between serum NT-proBNP, hs-cTnI, CysC and heart failure in acute myocardial infarction after PCI Objective To explore the correlation between serum N-terminal pro-brain natriuretic peptide (NT-proBNP), high-sensitive cardiac troponin (hs-cTnI), cystatin C (CysC) and heart failure (HF) in acute myocardial infarction (AMI) after percutaneous coronary intervention (PCI). Methods A total of 154 patients with AMI undergoing PCI in Guang’an People's Hospital were enrolled as AMI group between January 2020 and January 2022. According to presence or absence of HF at 6 months after PCI, they were divided into HF group (28 cases) and non-HF group (126 cases). A total of 77 healthy controls (HC) during the same period were enrolled as HC group. The levels of serum NT-proBNP, hs-cTnI and CysC between AMI group and HC group, as well as the clinical characteristics and levels of serum NT-proBNP, hs-cTnI and CysC between HF group and non-HF group were compared. The relationship among serum NT-proBNP, hs-cTnI and CysC was analyzed by Pearson correlation analysis. The risk factors of HF were analyzed by Logistic regression analysis. The diagnostic value of NT-proBNP, hs-cTnI and CysC for HF was analyzed by receiver operating characteristic (ROC) curves. Results The levels of serum NT-proBNP, hs-cTnI and CysC in AMI group were higher than those in HC group. There were significant differences in age, interval from onset to admission, number of coronary lesions, Gensini score, LVEF, Tei index and CK-MB between HF group and non-HF group. The levels of serum NT-proBNP, hs-cTnI and CysC in HF group were higher than those in non-HF group. Pearson correlation analysis showed that levels of serum NT-proBNP, hs-cTn and CysC were positively correlated. Logistic regression analysis showed that age, Gensini score, NT-proBNP, hs-cTnI and CysC were all influencing factors of HF. ROC curves analysis showed that AUC values of serum NT-proBNP, hs-cTnI and CysC in the diagnosis of HF were 0.878, 0.732 and 0.710, respectively. AUC, sensitivity and specificity of combined detection were 0.889, 94.74% and 75.76%, respectively. Conclusion The levels of serum NT-proBNP, hs-cTnI and CysC are closely related to HF in AMI patients after PCI, and the three indexes are correlated. 2023 Vol. 20 (1): 80-84 [Abstract] ( 505 ) [HTML 0KB] [PDF 1832KB] ( 1236 ) 85 LIU Xiaoting, JIANG Zhichao Mechanism on the Ginseng Lotion In the treatment of vaginitis Based on Network Pharmacology Objective To explore the mechanism of Ginseng Lotion in the treatment of vaginitis by network pharmacology, so as to provide a theoretical basis for later experimental research. Methods The active ingredients and their targets of ginseng lotion Decoction were screened by the Traditional Chinese Medicine Systems Pharmacology Database (TCMSP), vaginitis targets were obtained using GeneCards database. The R language is used to obtain a target set for the treatment of vaginitis by Ginseng Lotion. The target set uses to construct “drug-disease-target” network and the Protein-Protein interaction (PPI) network to exPlore its mechanism. Finally, Functional enrichment analysis of gene ontology (GO) and Pathway enrichment analysis based on the Kyoto EncycloPedia of Genes and Genomes (KEGG) were Performed using R language. Results There were 112 active comPounds and 83 targets related to the treatment of vaginitis in Ginseng Lotion. The key targets involved RELA、MAPK3、MYC、HIF1A、MAPK1、TP53, etc. It mainly involves TNF signaling pathway, IL-17 signaling pathway, HIF-1 signaling pathway and PI3K-Akt signaling pathway to Play a theraPeutic role in stomach cancer. Conclusion Through network pharmacology methods, it is concluded that Ginseng Lotion can treat vaginitis through multi-component, multi-target and multi-pathway. 2023 Vol. 20 (1): 85-91 [Abstract] ( 494 ) [HTML 0KB] [PDF 5048KB] ( 967 ) 92 HONG Lan, YANG Shanyi, SUI Weishu, YUAN Chiyu, YUAN Huabing, YIN Meilong, HU Yuzhang Meta-analysis of the relationship between milk intake and the incidence of children with allergic rhinitis Objective This study aims to investigate the effect of milk on the incidence of allergic rhinitis (AR). Methods English literature on the relationship between milk and AR from 2001 to 2019 was selected in PubMed, Embase and Cochrane Library. Literature was screened and quality score and data extraction were performed on the final literature. Stata 12.0 statistical software was used for meta-analysis. Results A total of 12 studies and 165, 966 patients were included. Milk intake was associated with AR (RR=1.05, 95% CI: 1.01-1.09), and subgroup results showed that milk intake increased the incidence of AR in Asian children (RR=1.24, 95% CI: 1.04-1.4 4) and in children older than seven years (RR=1.25, 95% CI: 1.05-1.46). Begg's (P=0.950) and Egger's (P=0.782) indicated that the relevant studies did not show publication bias. Conclusion The results of this study suggest that intake of milk is associated with an increased incidence of AR, but further studies are needed to confirm this. 2023 Vol. 20 (1): 92-96 [Abstract] ( 512 ) [HTML 0KB] [PDF 2757KB] ( 1195 ) 97 SHI Ying, DENG Xiaowen, SONG Guilin Correlation between clinical characteristics, hematological parameters and prognosis in patients with sudden unilateral total sensorineural hearing loss Objective To investigate the clinical characteristics and laboratory test results (such as hematological parameters) of patients with sudden unilateral total sensorineural hearing loss and to explore the factors affecting the curative effect of hearing recovery. Methods The clinical and laboratory examination data of 89 patients with sudden unilateral total sensorineural hearing loss hospitalized in Changsha Fourth Hospital Affiliated to Hunan Normal University from September 2019 to October 2022 were collected, and the gender, age, affected ears, time from onset to medical treatment, accompanied symptoms (tinnitus, dizziness, ear tightness), accompanied diseases (hypertension, diabetes, heart disease, hyperlipidemia) and the influence of hematological parameters (neutrophil number, lymphocyte number, fibrinogen, albumin, blood lipid) on prognosis. Results Univariate analysis showed that the time from onset to treatment, gender, affected ear, tinnitus, ear fullness, hypertension, diabetes, heart disease and hyperlipidemia were not related to the efficacy of patients with unilateral total sudden deafness. The effective rate of patients over 40 years old was 33.90%, which was significantly lower than 86.96% of patients under 40 years old. The effective rate of patients with vertigo was 25%, which was lower than 63.4% of patients without vertigo. Neutrophil count, lymphocyte count, platelet count, fibrinogen, albumin, triglyceride, low-density lipoprotein, FAR, PLR, NLR were not related to the prognosis of patients with unilateral total sudden deafness. There was a significant correlation between total cholesterol and high-density lipoprotein. Multivariate Logistic regression analysis of age, vertigo, total cholesterol and high-density lipoprotein showed that age was not related to the prognosis of patients with unilateral sudden deafness, while vertigo, total cholesterol and high-density lipoprotein were significantly related. Conclusion vertigo, total cholesterol and high-density lipoprotein are important risk factors for auditory recovery in patients with sudden unilateral total deafness. 2023 Vol. 20 (1): 97-100 [Abstract] ( 495 ) [HTML 0KB] [PDF 2213KB] ( 1181 ) 101 HONG Lun, YANG Yimei The relationship between the expression of T cells in peripheral blood and pulmonary function and quality of life in patients with silicosis Objective To investigate the relationship between peripheral blood T-cell Profiles and pulmonary function and quality of life in silicosis patients. Methods 90 patients who underwent silicosis hospitalization in our hospital from September 2019-March 2021 were enrolled in the silicosis group. At the same time, 90 healthy people who underwent physical examination at the same time were selected as healthy controls. The differences of peripheral blood T cell expression between the two groups were compared, and the relationship between peripheral blood T cell expression and lung function status and quality of life in silicosis group was analyzed. Results Compared with control subjects, patients with silicosis had lower FEV1, FEV1/FVC, DLco, CD4+T cells and CD4+/CD8+, and higher RV and CD8+T cells. There were significant differences in FEV1, FEV1/FVC, RV and SGRQ symptom score, activity score and total score among stageⅠ, Ⅱ and Ⅲ silicosis patients. The percentage of CD8+ T cells was negatively correlated with DLco, and positively correlated with symptom score and activity score. The percentage of CD4+ T cells was negatively correlated with FEV1 and FEV1/FVC. Conclusion The percentage of CD8+ T cells in peripheral blood of silicosis patients is positively correlated with the abnormal degree of gas exchange, while the percentage of CD4+ T cells is negatively correlated with the abnormal degree of gas exchange. 2023 Vol. 20 (1): 101-104 [Abstract] ( 467 ) [HTML 0KB] [PDF 1780KB] ( 1750 ) 105 CHEN Ling, HU Weicai, CHEN Weifeng, HUANG Ling, DENG Chunlei Observation on the therapeutic effect of isotretinoin capsules combined with ozone hydrotherapy method on acne Objective To observe the clinical effect of isotretinoic acid combined with ozone hydrotherapy on acne vulgaris. Methods 112 patients with acne vulgaris were retrospectively analyzed and divided into observation group (56 cases) and control group (56 cases). The patients in the control group received oral intervention with isotretinoic acid capsules, while the patients in the observation group received ozone hydrotherapy intervention on the basis of isotretinoic acid capsules, and the clinical effects of the two groups were observed. Results The total effective rate of patients in the observation group was 92.86%, significantly higher than 73.21% in the control group. The regression rate of white head acne, black head acne, sebum overflow, papules and pustules in the observation group was significantly higher than that in the control group. There was no significant difference in the incidence of adverse reactions between the two groups. Conclusion Isoretinoic acid combined with ozone hydrotherapy can significantly improve the efficacy of acne vulgaris and promote the regression of skin lesions, which is suitable for clinical application. 2023 Vol. 20 (1): 105-107 [Abstract] ( 448 ) [HTML 0KB] [PDF 1889KB] ( 1490 ) 108 LIU Xiaofu, PANG Jing Comparison of three detection methods in the early clinical diagnosis of osteoarticular tuberculosis Objective To investigate the clinical value of T-SPOT. TB, PPD and LAM-IgG in the early diagnosis of osteoarticular tuberculosis. Methods Ninety-one patients with suspected bosteoarticular uberculosis admitted from January 2019 to July 2021 were selected for the cell spot test (T-SPOT. TB), pure protein derivative (PPD) test and tuberculosis antibody (LAM-IgG) test to diagnose patients, compare the specificity, sensitivity, false positive rate (FPR) and other indicators of the three diagnostic methods, and evaluate their clinical value in the diagnosis of bone and joint tuberculosis. Results The sensitivity of T cell spot test was significantly higher than that of PPD test and tuberculosis antibody test (LAM-IgG), and the specificity was higher than that of PPD skin test. There was no significant difference between the specificity of T-SPOT.TB and LAM-IgG. The false positive rate of T-SPOT. TB was significantly lower than that of PPD test; There was no significant difference between the false-positive rate of T-SPOT.TB and LAM-IgG. Conclusion T-SPOT. TB has excellent sensitivity and specificity in the early diagnosis of osteoarticular tuberculosis and is an important auxiliary diagnostic index in the early diagnosis of osteoarticular tuberculosis. 2023 Vol. 20 (1): 108-110 [Abstract] ( 468 ) [HTML 0KB] [PDF 2148KB] ( 1457 ) 111 ZHANG Jin, BAI Lan, CHEN Siyu The relationship between RF, ESR, CRP and MCV and disease changes in RA patients Objective To investigate the relationship between serum rheumatoid factor (RF), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and mean corpuscular volume (MCV) and the changes of rheumatoid arthritis (RA) patients. Methods Selected 120 RA patients diagnosed in the First People's Court of Shuangliu District (RA group) and 120 healthy physical examination subjects (control group), the serum RF, ESR, CRP and MCV levels of the two groups were detected, and the RA group was tested according to the disease activity index (DAS28) group analysis. Results The levels of RF, ESR and CRP in the RA group were higher than those in the control group, and the MCV level in the RA group was lower than that in the control group; The levels of RF, ESR, and CRP in the RA group in the severe and mild active stages were higher than those in the stable stage. The MCV level of patients in the RA group in the severe active stage and mild active stage was lower than that in the stable stage patients; The levels of RF, ESR, and CRP in the RA group in the severe active stage were higher than those in the mild active stage, and the MCV level in the severely active RA group was lower than that in the mild active stage; The levels of RF, ESR and CRP in the RA group had a significant positive correlation with the DAS28 index, and the MCV level in the RA group had a significant negative correlation with the DAS28 index. Conclusion The levels of RF, ESR, and CRP in RA patients were increased, and the levels of MCV were decreased, and there was a certain correlation with the disease activity of patients. 2023 Vol. 20 (1): 111-113 [Abstract] ( 450 ) [HTML 0KB] [PDF 2389KB] ( 1305 ) Preventive Medicine 114 CHEN Can, ZHOU Caihong Study on current situation and Influencing factors of AIDS knowledge and life skills of students in school of medicine Objective To understand the current status of AIDS knowledge and anti-AIDS life skills among college students in a school of medicine located in Changsha, as well as to analyze the factors influencing the anti-AIDS life skills of college students. Methods The stratified whole-group sampling method was employed to extract college students from a school of medicine located in Changsha as the research subjects, so as to carry out a questionnaire survey regarding AIDS knowledge and anti-AIDS life skills. Meanwhile, the data analysis was conducted on the results, with P<0.05 as the difference being statistically relevant. Results The awareness rate of students in school of medicine about AIDS is 90.91%, their possession of anti-AIDS life skills is 51.08%, while their risk identification skills, negotiation skills, help-seeking skills and refusal skills are 82.68%, 55.41%, 11.68% and 64.94% respectively. The multivariate unconditional logistic regression analysis demonstrated that female students are 2.26 times (95% CI: 1.28-1.82) more likely than male students to be equipped with anti-AIDS life skills; 5.35 times (95% CI: 2.38-12.01) more likely to be in their fourth year than in their first year; 2.52 times more likely to be willing to perform HIV testing than those who are not (95% CI.1.07-5.94); 1.83 times more students in cities or towns than in rural areas (95% CI: 1.03-3.26). In addition, high AIDS knowledge (OR=1.36, 95% CI=1.02-1.82) scores serves as a facilitator of possessing anti-AIDS life skills among college students in school of medicine. Conclusion The college students in school of medicine are equipped with a relatively excellent level of AIDS knowledge, whereas the rate of possessing anti-AIDS life skills is comparatively low. It is imperative to further reinforce the training of anti-AIDS skills for students in school of medicine who are male, of lower grades, have no intention to conduct HIV testing, are of rural household registration, as well as with relatively insufficient level of AIDS knowledge. In the meantime, it is advisable to improve the level of AIDS knowledge among college students, to cultivate awareness of healthy behaviors, to further upgrade the anti-AIDS life skills of college students, in addition to advancing the implementation of the education on AIDS prevention and control in school of medicine. 2023 Vol. 20 (1): 114-118 [Abstract] ( 438 ) [HTML 0KB] [PDF 1767KB] ( 1436 ) Nursing Science 119 LI Jialing, CHEN Yuan, WU Shuang, PENG Qiang, LIU Weiming, WANG Weihong Status and influencing factors of drug literacy in rural elderly patients with cardiovascular diseases Objective To explore the current status and influencing factors of drug literacy in rural elderly patients with cardiovascular diseases. Method 486 rural elderly patients with cardiovascular diseases were selected by multistage random sampling. General data questionnaire, a self-administered Drug Literacy Questionnaire, Social Support Rating Scale and Self-efficacy scale for managing chronic disease were used to carry the survey, The multiple logistic regression analysis was used to explore the influencing factors of drug literacy. Results The total score of drug literacy among 486 rural elderly cardiovascular disease patients in Hunan province was (111.00 ± 12.36), the total social support score was (38.78 ± 7.26), and the chronic disease management self-efficacy score was (6.30 ± 1.77). The results of the Logistic regression analysis showed that age, level of social support, Self-care ability, and medication education were the influencing factors of medication literacy. Conclusion Drug literacy in rural elderly patients with cardiovascular disease was moderate, and Age over 80, low level of social support, lack of relevant drug education, and being unable to take care of themselves completely were risk factors for drug literacy in rural elderly patients with cardiovascular disease. 2023 Vol. 20 (1): 119-124 [Abstract] ( 486 ) [HTML 0KB] [PDF 1793KB] ( 1184 ) 125 PENG Yaqin, CHEN Ouying, ZHANG Juan, YIN Hongfang Relationship between Hypoglycemic Fear, Emotional Intelligence and Resilience in Type 2 diabetes Patients Objective To analyze the relationship between hypoglycemic fear, emotional intelligence and resilience in patients with type 2 diabetes (T2DM). Methods 186 patients with T2DM who experienced hypoglycemia were randomly selected from the Endocrinology Department of Hunan Provincial People's Hospital from April 2021 to April 2022. Questionnaires for general information, hypoglycemia fear scale II (CHFS II), emotional intelligence scale (WLEIS) and psychological resilience scale (CD-RISC) were distributed to the research subjects to investigate the general information, hypoglycemia fear, emotional intelligence of the patients, psychological resilience. To analyze the correlation of hypoglycemia fear with emotional intelligence and psychological resilience in T2DM patients. Results The total scores of CHFSⅡ, WLEIS and CD-RISC of 186 T2DM patients were (45.05±7.49), (60.68±7.15), and (53.38±6.73), respectively. The scores of CHFS Ⅱ, WLEIS and CD-RISC scales of T2DM patients with different age, education, work status and course of disease were significantly different (P<0.05). Pearson correlation analysis showed that T2DM patients' emotional control score (r=-0.534), self-emotional perception score (r=-0.581), emotional use score (r=-0.521), others' emotional perception score (r=-0.489), WLEIS total score (r=-0.545), tenacity score (r=-0.583), optimism score (r=-0.562), strength score (r=-0.541) The total score of CD-RISC (r=- 0.568) was negatively correlated with the total score of CHFS II (P<0.05). The results of multiple linear regression analysis showed that age, education level, course of disease, WLEIS total score and CD-RISC total score were the influencing factors of hypoglycemic fear in T2DM patients (P<0.05). Conclusion The T2DM patients admitted to our hospital have different degrees of fear of hypoglycemia, and both emotional intelligence and psychological resilience are related to it. By improving the level of emotional intelligence and psychological resilience, the fear of hypoglycemia in T2DM patients can be improved. 2023 Vol. 20 (1): 125-128 [Abstract] ( 512 ) [HTML 0KB] [PDF 2219KB] ( 1343 ) 129 SONG Caini, CHEN Huisu, HE Taolao, LIU Lihua The effect of social support on sleep and depression in COVID-19 survivors: the mediating role of anxiety Objective To explore the effect and mechanism of social support on sleep and depression in patients recovering from COVID-19, in order to provide a reference for improving sleep and depression in patients recovering from COVID-19. Methods The convenience sampling method was used to select 120 COVID-19 patients who donated convalescence plasma in Changsha, Hunan Province between January 2022 and July 2022. The investigation used a general information questionnaire, a social support scale, a self-rating anxiety scale, the Pittsburgh Sleep Scale, and a patient health questionnaire and 120 valid questionnaires were obtained. The mplus 8.7 structural equation model was used to investigate how anxiety affects social support, sleep, and depression. Results Result Anxiety, insomnia, and depression were all common among the patients. Anxiety was associated with poor sleep and depression. Anxiety was negatively correlated with social support; social support influences sleep and depression via anxiety’s mediating role. Conclusion Social support can help COVID-19 patients sleep better and feel better. 2023 Vol. 20 (1): 129-132 [Abstract] ( 511 ) [HTML 0KB] [PDF 1854KB] ( 1290 ) 133 LI Ling, HU Jiao, LI Qun, JIANG Jieqiong, CHEN Wei Mediating effect of self-compassion on the relationship between social support and diabetes distress in young and middle-aged patients with type 2 diabetes Objective To explore the mediating effect of self-compassion between social support and diabetes distress in young and middle-aged patients with type 2 diabetes. Methods A total of 225 young and middle-aged patients with type 2 diabetes from the endocrinology wards of 3 comprehensive tertiary hospitals in Hunan were recruited by convenience sampling. The questionnaires include Social Support Rating Scale (SSRS), Self-Compassion Scale (SCS), and Diabetes Distress Scale (DDS). SPSS25.0 and Process macro programs were used for statistical analysis. Results The scores of social support, self-compassion, and diabetes distress in young and middle-aged type 2 diabetes patients were (38.99±5.63), (72.95±8.49), and (36.28±7.39) respectively. Social support was negatively correlated with diabetes distress (r=-0.619, P<0.01) but positively correlated with self-compassion (r=0.577, P<0.01); self-compassion was negatively correlated with diabetes distress (r=-0.591, P<0.01). Self-compassion was the mediating variable of social support and diabetes distress, and the mediating effect accounted for 32.63% of the total effect. Conclusion Diabetes distress in young and middle-aged patients with type 2 diabetes was in the middle. Self-compassion is an intermediate variable between social support and diabetes distress in young and middle-aged patients with type 2 diabetes. Healthcare professionals should attempt to construct social support-based self-compassion intervention programs young and middle-aged patients with type 2 diabetes, so as to improve their level of self-compassion and thereby reduce their diabetes distress. 2023 Vol. 20 (1): 133-138 [Abstract] ( 562 ) [HTML 0KB] [PDF 1908KB] ( 1386 ) 139 LIU Jie, YAO Huiling, WU Xiaoyu, LI Guifei Construction of Medication Self-management Ability Questionnaire for the Community Home Elderly and Its Reliability and Validity Objective To develop a medication self-management ability questionnaire for the community elderly at home and to test the reliability and validity. Methods Guided by social cognitive theory, self-efficacy theory, and self-management theory, the initial questionnaire was formed by literature review, semi-structured interview, Delphi expert consultation, and pre-survey. From August to September 2022, a total of 334 older people at home with medication self-management in 4 community health service centers in Changsha were selected by multi-stage random sampling method to conduct a survey to test the reliability and validity of the questionnaire, and the score level of the questionnaire was classified. Results The questionnaire consisted of 27 items in 5 dimensions with a cumulative variance contribution of 52.388%. The item-level content validity index ranged from 0.800 to 1.000. The total Cronbach's α coefficient was 0.877, and the retest reliability was 0.903. The total score ranged from 27 to 135, among which the score of 27 to 92 recommended as low level, 93 to 107 as medium level, and 108 to 135 as high level. Conclusion The questionnaire developed in this study has good reliability and validity, which can be used to assess the medication self-management ability of community-dwelling older people. 2023 Vol. 20 (1): 139-144 [Abstract] ( 483 ) [HTML 0KB] [PDF 1858KB] ( 1064 )

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