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Quick Search     Advanced Search   2023 Vol.  20 No.  6 Published: 2023-12-25 Basic Medicine Clinical Medicine Preventive Medicine Nursing Science Invited Review Pharmacy Invited Review 1 LI Xiang, ZHENG Xi, GUO Yulan, XU Kun Research progress on the application of antimicrobial peptides in the detection of foodborne pathogenic bacteria Foodborne diseases caused by pathogenic bacteria are a serious threat to human health and a serious public health problem. Rapid and accurate detection of foodborne pathogenic bacteria in food is important for the prevention and control of foodborne diseases. In recent years, a variety of different highly sensitive and specific rapid detection methods have been developed with the aim of realizing the rapid detection of pathogenic bacteria in food. These methods all need to specifically identify bacteria from complex food matrixes. To achieve this goal, many small molecule substances have been developed, such as antibodies, aptamers, phages, antimicrobial peptides (AMPs), lectins, and enzymes. These small molecule substances have different advantages and disadvantages in application. The application of antimicrobial peptides has received more and more attention because of their high stability, simple synthesis, easy availability, high affinity for bacteria, low cost of AMPs compared with antibodies, DNA probes, more stable in harsh environments, and easy to be mass-produced. Therefore, they are being applied to the development and utilization of a variety of rapid detection methods. In this review, we systematically summarized the application of antimicrobial peptides in the detection of foodborne pathogens, and discussed its application prospects. 2023 Vol. 20 (6): 1-8 [Abstract] ( 221 ) [HTML 1KB] [PDF 2484KB] ( 1220 ) Basic Medicine 9 ZHANG Zhirong, WANG Weifan, XIAO Di, YANG Xiaoping Mechanism of Proguanil Enhancing the Inhibitory Effect of Sotorasib on Proliferation and Migration of KRASG12C Wild-type Bladder Cancer Cells Objective To explore whether proguanil can enhance the sensitivity of KRASG12C wild-type bladder cancer cells to sotorasib and its mechanism. Methods MTT, clone formation assay and Compusyn software were used to test whether the combination of sotorasib and proguanil can enhance the inhibition of KRASG12C wild-type bladder cancer cell proliferation and how the combined effect is. Scratch experiment was used to detect the inhibitory effect on cell migration, and finally, WB experiment was used to detect the inhibitory effect on proliferation related proteins. Results Sotorasib combined with proguanil enhanced the inhibition of proliferation and migration of KRASG12C wild-type bladder cancer cells. And the inhibitory effect on the expression of proliferation related proteins such as p-cRAF and p-ERK was enhanced. Conclusion Proguanil can enhance the sensitivity of KRASG12C wild-type bladder cancer cells to sotorasib by inhibiting RAF/ERK pathway. 2023 Vol. 20 (6): 9-13 [Abstract] ( 201 ) [HTML 1KB] [PDF 2698KB] ( 1252 ) 14 SUN Qian, WANG Jian, LIU Fen, YAN Sulan, PENG Jianqiang, ZOU Qiongchao Effect and mechanism of dapagliflozin on pulmonary arterial hypertension induced by monocrotaline in rats Objective To investigate the effect of dapagliflozin (DAPA) on monocrotaline (MCT)-induced pulmonary arterial hypertension (PAH) in rats and its mechanism of action. Methods Thirty male SD rats were randomly divided into three groups: (1) Control group, (2) Model group: pulmonary arterial hypertension group, (3) DAPA group: dapagliflozin treatment group. Single subcutaneous injection of MCT (60 mg/kg) was administered to establish the PAH model. The control group and the MCT group were given 0.5% sodium carboxymethyl cellulose by gavage every day, while the DAPA group was given dapagliflozin (1 mg/kg) dissolved in 0.5% sodium carboxymethyl cellulose by gavage. After 21 days, the right ventricular systolic pressure (RVSP) and right ventricular hypertrophy index (RVHI) of rats were measured, the percentage of pulmonary arteriolar wall thickness was measured by HE staining, pulmonary arterial α-smooth muscle actin was detected by immunohistochemistry, NLRP3, IL-1β, and IL-18 protein expression in lung tissue were detected by Western blot, and plasma IL-1β and IL-18 levels were measured by Elisa. Results DAPA significantly reduced RVSP and improved right ventricular hypertrophy in MCT-induced PAH rats, inhibited the thickening of pulmonary arterioles and decreased the degree of muscularization in PAH rats. Furthermore, DAPA inhibited the activation of the NLRP3 inflammasome in lung tissue and down-regulated the levels of IL-1β and IL-18 in lung tissue and plasma. Conclusion DAPA can reduce the RVSP, improve right ventricular hypertrophy and pulmonary vascular remodeling in MCT-induced PAH rats, which may be associated with its inhibition of NLRP3 inflammasome activation and downregulation of IL-18 and IL-1β expression levels. 2023 Vol. 20 (6): 14-19 [Abstract] ( 200 ) [HTML 1KB] [PDF 4029KB] ( 1281 ) 20 TANG Xilian, LI Ning, YU Huajun, HE Liuyan, HUANG Qianqian, CHEN Rongrong, CHEN Lili, WU Jun, ZHANG Haitao Preparation of succinylated antibody to the K89 position of AIF and study of the interacting proteins with AIF Objective Our research team has previously found that AIF protein exhibits increased levels of lysine 89 succinylation modification in non-smoking female lung adenocarcinoma patients. Our team aims to prepare an antibody that can recognize the succinylation modification of lysine 89 residue on AIF, which will be used to detect AIF succinylation at this site. Methods Bioinformatics analysis was conducted using online databases to analyze the physicochemical properties, transmembrane structure, signal peptide, protein structure, and succinylation sites of AIF protein. Based on the amino acid sequence of AIF, three peptides were synthesized, including the lysine 89 residue (K89) site. Among them, two peptides containing AIF K89 were succinylated. The three synthesized peptides were coupled with KLH and then immunized in rabbits to prepare antibodies specifically recognizing AIF K89 succinylation modification. The antibody efficacy and specificity were evaluated using ELISA, Dot blot, Western blotting, and immunohistochemistry. Gel digestion and high-resolution mass spectrometry were used to analyze the proteins interacting with AIF. Results Bioinformatics analysis revealed that succinylation modification of AIF K89 led to a change in the α-helical structure formed by amino acid residues 79-99 of AIF: it transformed from a longer α-helix into two shorter α-helices with a gap. The two synthesized succinylated peptides and one non-succinylated peptide successfully generated corresponding antibodies in rabbits. The antibody titers in the immune sera detected by ELISA were 1: 486, 000, 1: 162, 000, and 1: 18, 000, respectively. The prepared antibody recognizing AIF K89 succinylation modification exhibited 27 times higher efficacy in recognizing succinylated peptides compared to non-succinylated peptides, as determined by antibody Dot blot. Western blotting results showed that the AIF succinylation antibody specifically recognized the AIF K89 succinylation modification site. Mass spectrometry identified proteins interacting with AIF, including XIAP/BIRC4, EIF3G, and PRELID1. Conclusion Bioinformatics analysis suggests that succinylation modification of AIF K89 affects the structure of AIF protein. The prepared AIF K89 succinylation antibody can specifically recognize AIF K89 succinylation modification, providing a foundation for further investigation into the mechanism of AIF succinylation. 2023 Vol. 20 (6): 20-27 [Abstract] ( 186 ) [HTML 1KB] [PDF 3109KB] ( 1422 ) 28 WU Jia, WU Jin, XIAO Kai, LING Chao The improvement effect of dapagliflozin with heart failure based on SIRT1/AMPK pathway Purpose To investigate the improvement effect of dapagliflozin with heart failure based on SIRT1/AMPK pathway. Methods Animal TAC models were developed in C57BL/6J mice and divided into sham, sham+DAPA, TAC, and TAC+DAPA groups. Gravimetric and histological analysis of organ size and weight, HE staining, WGA staining, Masson, Sirius Red staining, DHE staining and TUNEL assay to analyze histopathology, cardiomyocyte size, collagen fibers, ROS production and apoptosis, qRT- The expressions of atrial natriuretic factor (Nppa), brain natriuretic peptide (Nppb), myosin heavy chain 7(Myh7), TGF-β1 and SIRT1/AMPK signaling pathway were detected by PCR and western blotting. Results CHW/TL, HW/BW and HW/LW were significantly reduced in DAPA TAC mice compared with sham operation group. As assessed by WGA staining, the mean cross-section area of cardiocytes in TAC mice was larger. Increases in Nppa, Nppb, and Myh7, in addition to high levels of ANP and β-MHC in TAC mice, decreased mean cardiomyocyte cross-sectional area was observed in DAPA-administered TAC mice. mRNA and protein levels of markers of cardiac hypertrophy were significantly reduced. Masson and Sirius red were used to examine the degree of myocardial fibrosis. Compared with the sham operation group, the myocardial fibrosis area of TAC mice was significantly increased, the mRNA and protein levels of TGF-β1 in TAC group were increased, and the myocardial ROS levels of TAC mice were higher. TUNEL staining showed that the apoptosis rate of cardiomyocytes in TAC group was significantly increased, and the expression of SIRT1/AMPK pathway was decreased, but DAPA had the opposite effect, the difference was statistically significant. ompared with the sham-operated group, HW/TL, HW/BW and HW/LW were significantly reduced in TAC mice with DAPA, and the mean cross-sectional area of cardiomyocytes in TAC mice was large, Nppa, Nppb and Myh7 as assessed by WGA staining In addition, TAC mice had high levels of ANP and β-MHC, and a reduction in mean cardiomyocyte cross-sectional area and significantly lower mRNA and protein levels of cardiac hypertrophy markers were observed in DAPA-administered TAC mice. Masson and Sirius Red examined the degree of myocardial fibrosis. Compared with the sham-operated group, the myocardial fibrosis area of TAC mice was significantly increased, the mRNA and protein levels of TGF-β1 in the myocardium of the TAC group were increased, and the myocardial ROS level of TAC mice TUNEL staining showed that the apoptosis rate of cardiomyocytes in the TAC group was significantly increased, and the expression of SIRT1/AMPK pathway was decreased, but the effect of DAPA was the opposite, and the difference was statistically significant. Conclusion DAPA activates SIRT1/AMPK pathway signaling, thereby reducing cardiac fibrosis, hypertrophic remodeling, and myocardial oxidative stress. 2023 Vol. 20 (6): 28-34 [Abstract] ( 222 ) [HTML 1KB] [PDF 3018KB] ( 1102 ) 35 HE Bin, HU Guohuang, XIA Dan lncRNA PCBP1-AS1 Aggravates the EMT of Hepatocellular Carcinoma via Regulating PCBP1/miR-199/XBP1 Pathway Objective This study aims to investigate the regulatory role of lncRNA PCBP1-AS1 in EMT transformation of hepatocellular carcinoma and its possible mechanism, so as to provide a new target for the prevention and treatment of hepatocellular carcinoma. Methods Real-Time PCR was used to detect the expression of lncRNA PCBP1-AS1, miR-199, XBP1 and other genes in liver tissue of HCC patients or HCC cells. Pearson correlation coefficient was used to analyze the correlation between lncRNA PCBP1-AS1 expression and TNM stage of hepatocellular carcinoma. Bioinformatics was used for predicting the interaction between lncRNA PCBP1-AS1 and miR-199, which was verified via luciferase reporter gene experiment. Lipo2000 was used for transfering PCBP1-AS1 plasmid or miR-199 mimc (inhibitor) into Hep3B cells. Transwell assay was used for detecting the invasive ability of cells. Scratch test was used for detecting the migration ability of cells. CCK-8 kit was used for detecting the cell activity. LDH release assay was used for detecting the cytotoxicity. Western blot was used for detecting the expression of XBP1 and other genes. Results Compared with the normal liver tissues, the expression level of LncRNA PCBP1-AS1 in liver cancer tissues was significantlyincreased. The expression level of LncRNA PCBP1-AS1 was positively correlated with the TNM stage of hepatocellular carcinoma. It was predicted that lncRNA PCBP1-AS1 interacted with miR-199 mimic, and miR-199 mimic could significantly reduce the luciferase activity of Hep3B cells transfected with lncRNA PCBP1-AS1-wt luciferase reporter gene. LncRNA PCBP1-AS1 overexpression of can significantly increase the expression of lncRNA PCBP1-AS1, XBP1, Snai1 and Vimentin in Hep3B cells, significantly decrease the expression of miR-199 and E-cadherin, significantly increase the viability of H cells, and significantly reduce LDH release, significantly increase the migration and invasion ability of Hep3B cells, and these effects could be inhibited by miR-199 mimic or promoted by miR-199 inhibitor. Conclusion LncRNA PCBP1-AS1 can upregulatethe expression of Snai1 and Vimentin, downregulate the expression of E-cadherin in liver cancer cells and promote its epithelial-mesenchymal transformation (EMT) via sponging the expression of miR-199. LncRNA PCBP1-AS1 is a potential target for hepatocellular carcinoma. 2023 Vol. 20 (6): 35-43 [Abstract] ( 192 ) [HTML 1KB] [PDF 6596KB] ( 1044 ) 44 CHEN Jiaofeng, CHEN Shengyuan, YU Jing The mechanism of SIRT1/PGC-1α pathway on skin flap survival and autophagy in rat severed finger replantation Objective To explore the role and possible mechanism of silencing information regulatory factor 1(SIRT1) /peroxisome proliferator-activated receptor-γ-coactivator 1α (PGC-1α) signaling pathway in replanted flaps survival and autophagy in rat finger replantation. Methods According to the experimental design, 50 SD rats were randomly divided into control group, SIRT1 agonist (SRT-1460) group, rapamycin (RAPA) group, RAPA+SIRT1 inhibitor (EX527) group and SRT-1460+RAPA group, with 10 rats in each group. The survival rate and the survival area of the skin flap were observed in each group. The expression levels of SIRT1, PGC-1α, Beclin1, microtubule-associated protein 1 light chain 3II (LC3II) in the skin flap of the rats in each group were detected by Western blotting. The mRNA expression levels of SIRT1, PGC-1α, Beclin1 and LC3II in rat skin flap were detected by RT-qPCR. Results Compared with the control group, the survival rate (74.81±4.38) and the survival area (6.22±1.15) of the rat skin flap in SRT-1460 group were significantly increased, the protein and mRNA expressions of SIRT1, PGC-1α, Beclin1, LC3II were significantly increased in rat skin flap after severed finger replantation, the survival rate (76.50±5.82) and the survival area (6.94±2.61) were increased in RAPA group, the protein and mRNA expressions of SIRT1, PGC-1α, Beclin1, LC3II were significantly increased in rat skin flap after severed finger replantation. Compared with RAPA group, the survival rate (67.23±6.28) and the survival area (5.91±1.83) of the rat skin flap in RAPA+EX527 group were decreased, the protein and mRNA expressions of SIRT1, PGC-1α, Beclin1, LC3II were significantly decreased. Compared with SRT-1460 group, the survival rate (89.71±7.13) and the survival area (8.32±1.26) of rat skin flap in SRT-1460+RAPA group were decreased, the protein and mRNA expressions of SIRT1, PGC-1α, Beclin1, LC3II were significantly decreased. Conclusion SIRT1/PGC-1α signaling pathway plays an important role in the survival of rat skin flap after finger replantation. Inhibition of this pathway can reduce autophagy and the survival of rat skin flap after finger replantation. Activation of this pathway can enhance autophagy and improve the survival of rat skin flap after finger replantation. 2023 Vol. 20 (6): 44-48 [Abstract] ( 169 ) [HTML 1KB] [PDF 2562KB] ( 1413 ) 49 DENG Jun, LIU Wen Down-regulating SCD1 expression sensitizes RSL3 induced ferroptosis and inhibited bladder cancer cell growth Objective To explore the effect and mechanism of SCD1 protein expression on the ability of ferroptosis activator RSL3 to induce ferroptosis in bladder cancer cells. Method T24 cells were treated with SCD1 small interfering RNA (siRNA), specific SCD1 inhibitor (A9) or combined with RSL3; The effects of inhibiting SCD1 expression or combining with RSL3 on cell proliferation and clonogenesis were detected by CCK-8 and clonogenesis assay; Malondialdehyde (MDA) and lipid peroxides (LPO) were determined to investigate the effect of down-regulated SCD1 on the ability of RSL3 to induce ferroptosis in bladder cancer cells T24; Finally, glutathione peroxidase 4(GPX4) expression was detected by western blot. Results Both siRNA silencing and A9 treatment decreased the proliferation and colony formation ability of bladder cancer cells. Compared with the single treatment group, The two SCD1 inhibition methods combined with RSL3 further increased the MDA and LPO content in the cells, and enhanced the degree of inhibition on the proliferation and clonal colony formation of bladder cancer cells. In addition, inhibition of SCD1 enhanced the inhibitory effect of RSL3 on GPX4 protein expression. Conclusion Down-regulation of SCD1 can inhibit the growth of bladder cancer cells by enhancing the inhibition of GPX4 protein expression by RSL3 and inducing ferroptosis. 2023 Vol. 20 (6): 49-54 [Abstract] ( 185 ) [HTML 1KB] [PDF 8334KB] ( 1213 ) Clinical Medicine 55 LI Bei, CHEN Yuan, ZHANG An Analysis of Factors Influencing Improvement in Prostate Symptoms After Benign Prostatic Hyperplasia Surgery Objective This study aimed to analyze the factors influencing improvement in prostate symptoms in patients with benign prostatic hyperplasia (BPH) treated with either bipolar transurethral resection of the prostate (BPRP) or holmium laser enucleation of the prostate (HoLEP). Methods We conducted a retrospective analysis of data from 240 patients diagnosed with BPH and treated between October 2018 and October 2021 at Tongji Hospital, Wuhan. Preoperative and postoperative data were reviewed, including urodynamic parameters in some patients, International Prostate Symptom Score (IPSS), Overactive Bladder (OAB) symptoms, Quality of Life (QoL) scores, surgical approach, and perioperative information. A telephone follow-up was performed at 6 months post-surgery to record relevant follow-up data. Among the 240 patients, 124 completed urodynamic examinations, and data from these patients were statistically analyzed to explore factors influencing postoperative improvement in prostate symptoms. Improvement in IPSS was defined as (postoperative/preoperative) ≤0.50, and patients were divided into the Improvement Group (Group A, 190 cases) and the Poor Improvement Group (Group B, 50 cases). Results All 240 patients who met the inclusion criteria had successful surgeries, with 178 patients undergoing HoLEP and 62 patients undergoing BPRP. Age, disease duration, BMI, FBG, TC, TG, AST, ALT, ALB, Csr, GFR, postoperative IPSS, preoperative and postoperative QoL, preoperative and postoperative OAB, surgical duration, indwelling catheter time, length of hospital stay, preoperative urinary retention, history of cardiovascular disease, history of cerebral infarction, history of diabetes, surgical approach, Qmax, FDV, PVR, Pqmax, SDV, BOOI, and BCI were not identified as risk factors for poor postoperative IPSS improvement. Prolonged disease duration (P<0.001), prostate volume (PV) (P=0.024), total prostate-specific antigen (TPSA) (P=0.002), free prostate-specific antigen (FPSA) (P=0.006), elevated preoperative IPSS (P=0.015), and decreased hemoglobin (Hb) (P=0.016) were identified as risk factors for poor postoperative IPSS improvement, with preoperative IPSS (P=0.006) and elevated PV (P=0.035) being independent risk factors. Conclusion Patients with larger prostate volumes and higher preoperative IPSS scores may experience poorer IPSS improvement after BPRP or HoLEP treatment for BPH. These patients should be counseled before surgery regarding the possibility of persistent prostate symptoms postoperatively, and medication may be necessary to alleviate symptoms if required. 2023 Vol. 20 (6): 55-59 [Abstract] ( 178 ) [HTML 1KB] [PDF 1957KB] ( 1336 ) 60 XIANG Shiqi, ZHANG Zhou, GAO Xiaoping, YANG Jianfeng The value of endovascular recanalization in patients with non-acute large artery occlusion with impaired anterior circulation perfusion Objective Investigating the improvement of imaging and clinical symptoms of endovascular recanalization for large artery occlusion (LAO) with impaired anterior circulation perfusion and evaluating the safety and efficacy of endovascular opening therapy in multiple dimensions. Methods patients with non-acute LAO with impaired anterior circulation perfusion in Hunan Provincial People’s Hospital were included from January 2020 to February 2021, who were all treated with endovascular recanalization. We compared the multimode imaging and neurology scales between preoperative and postoperation to explore the safety and effectiveness of the treatment. Results A total of 13 patients were accept this study, including 6 cases with MCAO and 7 cases with ICAO.11 patients were successfully operated. Preoperative CTP showed that compared with that in the healthy side, The neurological deficits and functional independence rate at 30 days after operation、the cognitive function at 90 days after surgery were all improved significantly compare with preoperative. In addition, the radiographic follow-up some patients showed that no restenosis occurred in the patients with successful recanalization, and with the gradual recovery of hemodynamics, the absolute values of CBF, MTT and TTP tended to be normal. Conclusion Endovascular recanalization for LAO with impaired anterior circulation perfusion is feasible, relatively and relatively safe, improving patients’overall prognosis and quality of life. Further larger scale studies are needed to evaluate the efficacy and long-term outcome associated with this treatment. 2023 Vol. 20 (6): 60-63 [Abstract] ( 152 ) [HTML 1KB] [PDF 1909KB] ( 1340 ) 64 ZHU Xuhua, FU Dongmei, HUANG Yuxiao Effects of Zishen Huoxue Antai decoction on hormone levels and uterine artery abnormalities in patients with recurrent abortion of kidney deficiency and blood stasis type Objective To study the effects of Zishen Huoxue Antai decoction on hormone levels and uterine artery abnormalities in patients with recurrent abortion of kidney deficiency and blood stasis type. Methods A total of 80 patients with recurrent abortion of kidney deficiency and blood stasis type admitted to Beijing Daxing District Maternal and Child Health Hospital from March 2019 to March 2022 were randomly divided into the conventional group（40 cases）and the traditional Chinese medicine group（40 cases）by random number table method. The conventional group received routine fetal preservation treatment,and was treated with dydrogesterone,vitamin E capsules,and low molecular weight heparin;the traditional Chinese medicine group was treated with Zishen Huoxue Antai decoction on the basis of the conventional group. Both groups were treated for 45 d,and the patients were followed up until delivery. The clinical efficacy of the two groups after 45 d of treatment and the pregnancy outcome during follow-up were analyzed,and the traditional Chinese medical syndrome scores,serum level of hormone,coagulation function,uterine artery and endometrial abnormalities were compared between the two groups before and after 45 d of treatment. Results After 45 d of treatment,the total effective rate of the traditional Chinese medicine group（92.50%）was higher than that of the conventional group（72.50%）;During the follow-up period,the success rate of pregnancy in the traditional Chinese medicine group（82.50%）was higher than that in the conventional group（62.50%）. During the follow-up period,the pregnancy success rate in the traditional Chinese medicine group was higher than that in the conventional group. After 45 d of treatment compared with before treatment,The scores of nocturnal frequent urination,lumbar and knee tenderness,cold limbs,dark face,dizziness and tinnitus,serum levels of D-D,FIB,PAI-1,uterine artery and endometrial PI,RI,S/D decreased in both groups. The traditional Chinese medicine group was lower than the conventional group. Serum levels of E2,P and β-HCG in two groups increased,and those in the traditional Chinese medicine group were higher than those in the conventional group. The aPTT in two groups was longer,and those in the traditional Chinese medicine group were longer than those in the conventional group. Conclusion Zishen Huoxue Antai decoction combined with routine abortion treatment could improve the traditional Chinese medical syndrome of patients with recurrent abortion of kidney deficiency and blood stasis,regulate the level of hormone,improve the coagulation function of patients,promote uterine artery and endometrial blood perfusion,improve the pregnancy outcome of patients,and the curative effect was good. 2023 Vol. 20 (6): 64-69 [Abstract] ( 190 ) [HTML 1KB] [PDF 2292KB] ( 1253 ) 70 GUO Xiaoyang, HE Yue, DENG Xiaoxiu, PAN Qingdeng, BAO Huixian Study on the clinical effect of self-proposed Shenfu Zhenqi Tang combined with rivaroxaban in the treatment of chronic heart failure Objective To observed the clinical effect of self-proposed Shenfu Zhenqi Tang combined with rivaroxaban in treating chronic heart failure. Methods 80 patients with chronic heart failure were randomly divided into the control group and the observation group, and the control group received conventional treatment + rivaroxaban treatment. The observation group received conventional treatment + rivaroxaban. The observation group was treated with self-proposed Shenfu Zhenqi Tang based on the control group. The two groups were compared regarding improvement of major clinical symptoms, serum NT-pro BNP level, 6-min walking distance, improvement of cardiac function, and therapeutic effect. Results The disappearance time of clinical symptoms such as palpitation and shortness of breath in the observation group was shorter than in the control group. There was no statistically significant difference between the two groups in the disappearance time of clinical symptoms such as facial limb swelling, dry mouth, shortness of breath, chest tightness, and cough. The serum NT-pro BNP level after treatment in the observation group was lower than that in the control group. The 6-minute walking distance after treatment in the observation group was much longer than that in the control group. After treatment, LVESD, LVEDD, and LVRT of the observation group were significantly lower than those of the control group, and EF was significantly higher than that of the control group. The total effective rate after treatment in the observation group was higher than in the control group. Conclusion Self-designed Shenfu Zhenqi Tang combined with rivaroxaban in treating of chronic heart failure can reduce clinical symptoms, lower plasma NT-pro BNP level, increase 6-minute walking distance, improve myocardial function, and be suitable for clinical application. 2023 Vol. 20 (6): 70-72 [Abstract] ( 239 ) [HTML 1KB] [PDF 2281KB] ( 1323 ) 73 LI Xiaoli, PU Qiang, LV Jun Effect of Ruangan Jiangzhi Capsule on Vascular Endothelial Function and Platelet Function in Patients with Acute Coronary Syndrome Objective To study the effect of Ruangan Jiangzhi capsule on vascular endothelial function and platelet function in patients with acute coronary syndrome. Methods A total of 120 patients with acute coronary syndrome admitted to Rugao Hospital of Traditional Chinese Medicine affiliated to Nanjing University of Chinese Medicine from July 2021 to July 2022 were divided into the secondary prevention treatment group (60 cases) and combined the combined the Chinese medicine group (60 cases) by random number table method. The secondary prevention and treatment group was treated with standard secondary prevention drugs for coronary heart disease, and the combined Chinese medicine group was treated with Ruganjiangzhi capsule on the basis of the secondary prevention and treatment group, and both groups were treated for 6 months. The traditional Chinese medicine symptom score, cardiac function, serum vascular endothelial function index level, serum inflammatory factors, lipid metabolism index level, platelet function index, aspirin resistance and clopidogrel resistance were compared between the two groups, and the safety of the two groups was counted. Results After 6 months of treatment, compared with before treatment, the score of traditional Chinese medicine symptom, cardiac function and level of serum vascular endothelial function of the two groups were significantly improved, and the improvement degree of the combined Chinese medicine group was better than that of the secondary prevention treatment group . Before treatment and after 1 and 6 months of treatment, the levels of serum inflammatory factors and platelet function indexes in the two groups were significantly improved, and the improvement degree in the combined Chinese medicine group was better than that in the secondary prevention treatment group after 1 and 6 months of treatment . Before treatment and after 1, 3 and 6 months of treatment, the serum lipid metabolism indexes of the two groups were obviously improved, and the improvement degree of the combined Chinese medicine group after 1, 3 and 6 months of treatment was better than that of the secondary prevention treatment group . After 6 months of treatment, the aspirin resistance rate and clopidogrel resistance rate of the two groups increased compared with 1 month of treatment, and the combined Chinese medicine group was lower than the secondary prevention treatment group after 1 and 6 months of treatment . Conclusion Ruangan Jiangzhi Capsule could improve traditional Chinese medicine syndrome, cardiac function, vascular endothelial function and platelet function of patients with acute coronary syndrome, reduce the risk of inflammatory reaction, aspirin resistance and clopidogrel resistance, promote lipid metabolism, and at the same time, it would not increase the occurrence of adverse reactions, with good therapeutic effect. 2023 Vol. 20 (6): 73-78 [Abstract] ( 169 ) [HTML 1KB] [PDF 2012KB] ( 1154 ) 79 LI Yongchang, ZHOU Lin, FU Ke, PENG Yi, HE Cai, YU Xiang Analysis of stroke risk factors of Han and Uyghur in Turpan area Objective To statistically analyze the distribution characteristics of stroke risk factors in Han and Uygur populations in Turpan area. Methods From January 2021 to December 2021,people aged 40 and above（3269 Han and Uyghur people in total）in the Physical examination center database of Turpan People's Hospital were selected as the research objects. The statistical analysis data included general information,laboratory test results and past history. Result The prevalence of hypertension in Uyghur Chinese nationality aged 41-50 years is significantly lower than that in Hannationality,while the prevalence of hypertension in Uyghur nationality aged 71-80 is significantly higher than that in Han nationality. The smoking rate of Uyghur nationality aged 51-60 is significantly higher than that of Han nationality;The prevalence of diabetes among Uyghur nationality aged 71-80 is lower than that of Han nationality;The overweight rate of Uyghur men and women aged 41-80 was significantly higher than that of Han nationality group,while there were statistical differences in the 41-50 age groups and 51-60 age groups. The drinking rate of Uyghur nationality aged 61-70 and 71-80 is significantly lower than that of Han nationality. Conclusion There are differences in the distribution of stroke risk factors among Han and Uygur Chinese nationalityin Turpan area,and we need to monitor and intervene on stroke risk factors according to ethnicity,gender and other factors. 2023 Vol. 20 (6): 79-86 [Abstract] ( 167 ) [HTML 1KB] [PDF 5692KB] ( 1358 ) 87 SHEN Xiaojing, LI Fang, SHI Xunting Correlation of PI3K/AKT signaling pathway-related factors with abnormal glucose tolerance and insulin resistance in women during pregnancy Objective To investigate the correlation between factors related to the phosphatidylinositol 3-kinase (PI3K) /serine threonine protein kinase (AKT) signaling pathway and abnormal glucose tolerance and insulin resistance in women during pregnancy. Methods Seventy-five pregnant women with abnormal glucose tolerance during pregnancy in our hospital from March 2020 to March 2022 were selected as the study group, and another 75 pregnant women with normal glucose tolerance during the same period were selected as the control group. The clinical data, PI3K/AKT signaling pathway markers [PI3K, phosphorylated AKT (p-AKT) ], fasting blood glucose (FPG), insulin resistance index (Homa - insulin resistance, HOMA-IR) were compared between the two groups, and the effects of PI3K and p-AKT on abnormal glucose tolerance in women during pregnancy were analyzed by interaction. Pearson correlation coefficient was used to analyze the correlation between PI3K and p-AKT levels and FPG and HOMA-IR in pregnant women with abnormal glucose tolerance during pregnancy. The clinical data and serum PI3K, p-AKT, FPG levels and HOMA-IR of pregnant women with simple glucose tolerance abnormalities and pregnant women with gestational diabetes mellitus (GDM) in the study group were compared, and the factors influencing GDM were analyzed by logistic multi-factor regression model, and the value of serum PI3K and p-AKT levels in predicting GDM was analyzed by subject work characteristic (ROC) curve. RESULTS: Serum PI3K levels were lower in the study group than in the control group, and serum p-AKT, FPG levels and HOMA-IR were higher than in the control group; low PI3K levels and high p-AKT levels showed a positive interaction in a superphase multiplicative model of glucose tolerance abnormalities in pregnant women; serum PI3K levels in pregnant women with glucose tolerance abnormalities in pregnancy were negatively correlated with FPG, HOMA-IR, and p-AKT levels were negatively correlated with FPG and HOMA-IR. Body mass index, family history of diabetes, serum p-AKT, FPG levels and HOMA-IR were higher in pregnant women with GDM than in pregnant women with simple glucose tolerance abnormalities, and serum PI3K levels were lower than in pregnant women with simple glucose tolerance abnormalities; body mass index, family history of diabetes, serum PI3K and p-AKT levels were all influencing factors for GDM; serum The area under the curve (AUC) for the prediction of GDM by serum PI3K and p-AKT levels were all>0.7, and the AUC for the combined prediction of the two was the largest at 0.916, and the sensitivity and specificity of the prediction were 90.62% and 80.13%, respectively. Conclusion Changes in the levels of PI3K and p-AKT, markers of PI3K/AKT signaling pathway, are closely associated with abnormal glucose tolerance and insulin resistance in women during pregnancy, and their combination has good predictive efficacy in predicting GDM. 2023 Vol. 20 (6): 87-91 [Abstract] ( 223 ) [HTML 1KB] [PDF 2722KB] ( 1345 ) 92 WANG Lingyan, CAO Guojun, GONG Tian, ZHOU Shijing Efficacy of ginkgo leaf extract dipyridamole injection combined with alprostadil in the treatment of patients with vertebrobasilar insufficiency vertigo and its influence on hemodynamics Objective To mainly explore the influence of ginkgo leaf extract dipyridamole injection and alprostadil on patients with vertebrobasilar insufficiency vertigo (VBIV). Methods 150 patients with VBIV were selected from May 2019 to April 2023, and they were divided into control group and observation group by the odd-even number method, with 75 cases in each group. Control group was treated with alprostadil (PGE1), and observation group was given PGE1 combined with ginkgo leaf extract dipyridamole. The clinical efficacy after 2 weeks of treatment, hemodynamics [left vertebral artery (LVA) flow velocity, right vertebral artery (RVA) flow velocity, basal artery flow velocity (BA) ], hemorheology [erythrocyte sedimentation rate (ESR), hematocrit (HCT), whole blood high-shear viscosity (HSV), whole blood low-shear viscosity (LSV) ], oxidative stress indicators [serum superoxide dismutase (SOD), nitric oxide (NO), malondialdehyde (MDA), glutathione peroxidase (GSH-Px) ], inflammatory factors [hypersensitive C-reactive protein (hs-CRP), interleukin-6(IL-6) ] and vertigo degree[Visual Vertigo Analogue Scale (VVAS), dizziness handicap inventory (DHI) ] before treatment and after 2 weeks of treatment and drug safety during treatment were compared between both groups. Results After 2 weeks of treatment, the total effective rate of efficacy in observation group was significantly higher compared with that in control group. The LVA, RVA, BA, SOD, NO and GSH-Px in both groups after 2 weeks of treatment were enhanced compared with those before treatment, and the indicators were higher in observation group. The ESR, HCT, HSV, LSV, MDA, hs-CRP and IL-6 were reduced after 2 weeks of treatment in the two groups, and the indicators in observation group were lower in comparison with control group. After 2 weeks of treatment, the scores of VVAS and DHI in both groups were significantly decreased compared with those before treatment, and the scores in observation group were lower compared to control group. During treatment, the differences were not significant in the incidence rates of adverse reactions between the two groups. Conclusion Ginkgo leaf extract dipyridamole injection combined with alprostadil has a significant effect in treating VBIV, and it can effectively restore the hemodynamics and hemorheology, relieve the inflammatory response and oxidative stress response, and help improve vertigo symptoms, with high safety. 2023 Vol. 20 (6): 92-96 [Abstract] ( 205 ) [HTML 1KB] [PDF 1965KB] ( 1016 ) 97 WU Jichu, JIANG Xiaoye, SHEN Sizhe The expression and clinical significance of HOXC8 in colon cancer Objective To investigate the expression and clinical significance of Homeobox C8 (HOXC8) in colon cancer (CC) . Methods The Cancer Genome Atlas (TCGA) database and GEO database were used to analyze HOXC8 mRNA expression in CC tissues. A total of 100 patients with CC tissue specimens and clinicopathological data were collected from Chongqing Medical University First Affiliated Hospital, and HOXC8 protein was detected by immunohistochemistry. Moreover, the association between HOXC8 expression and clinicopathological characteristics was analyzed. Univariate and multivariate Cox regression models and Kaplan-Meier were used to evaluate the prognostic value of HOXC8 in CC. The GEPIA database was used to evaluate the correlation between HOXC8 and immune cell biomarkers. Results Multi databases analysis and detection of patient tissue samples showed that the mRNA and protein expression level of HOXC8 is increased in cancer tissue (P<0.05, P<0.001) . In addition, the high expression of HOXC8 is related to poor overall survival rate (In TCGA data set, P<0.001; In GEO GSE39582 data set, P=0.008) . Cox regression analysis suggests that HOXC8 is an independent risk factor affecting the prognosis of patients with CC (In TCGA data set, P=0.002; In GEO GSE39582 data set, P= 0.039) . We also studied the potential role of HOXC8 in tumor-immune interactions, and found that HOXC8 may be significantly associated with multiple immune cells in colon cancer (P< 0.001) . Conclusion Our study showed that the expression of HOXC8 is up-regulated in CC, and revealed the prognostic potential of HOXC8 in CC and its potential as an immune-related therapeutic target for CC. 2023 Vol. 20 (6): 97-104 [Abstract] ( 189 ) [HTML 1KB] [PDF 3026KB] ( 963 ) 105 ZHANG Ziyu, WEN Juan, XIE Fen, PENG Hongying, Duan Wen, YU Caihong Clinical efficacy of atorvastatin calcium tablets combined with sacubitril/valsartan in patients with chronic heart failure Objective The aim of this report is to explore the effects of atorvastatin calcium tablets combined with sacubitril/valsartan on clinical efficacy, cardiac color ultrasound indexes and serum indexes in patients with chronic heart failure. Methods We collected data of 87 patients with chronic heart failure admitted to Sanzhen Rehabilitation Hospital in Changsha, Hunan Province from January 2022 to December 2022. According to the random number table method, the patients were divided into control group (treated with atorvastatin calcium tablets orally and diuretics, etc. ) with 43 cases and observation group (treated with sacubitril/valsartan plus the same therapy as the control group) with 44 cases. Results were compared between the two groups, including post-therapy efficacy and readmission rates, cardiac ultrasound and serum indexes before and after treatment, and the occurrence of adverse reactions during treatment. Results After 2 months of treatment, the total clinical efficiency of the observation group was 95.45%, which was significantly higher than that of the control group (74.42%). And the overall incidence of adverse reactions and the readmission rate of the observation group were observably lower than those of the control group. Compared with the pre-therapy period, an increase of various indicators was significantly obvious in both groups after treatment, including left ventricular ejection fraction (LVEF), systolic peak velocity (SPV), left anterior descending branch diastolic peak velocity (DPV) and time velocity integral (TVI). And the changes in the observation group were signally more obvious than the control group, with statistically significant differences between the two groups. Both of the two groups had an evident decrease in the left ventricular end-systolic diameter (LVESD) and the left ventricular end-diastolic diameter (LVEDD). And the LVESD and LVEDD were lower in the observation group than in the control group. However, there was no statistically significant difference in LVEDD level through the intra-group comparison of the control group before and after treatment. Conclusion Atorvastatin calcium tablets combined with sacubitril/valsartan has a good application effect in patients with chronic heart failure, as it apparently contributes to improving the prognosis and enhancing the quality of their lives. 2023 Vol. 20 (6): 105-107 [Abstract] ( 230 ) [HTML 1KB] [PDF 2280KB] ( 1199 ) 108 XI Lei, SHI Ye, CUI Weiming, CAO Hui Effect of single-port thoracoscopic segmentectomy on lung function, and disease free survival in patients with early NSCLC Objective To explore the effect of single-port thoracoscopic segmentectomy on lung function, serum trauma response and long-term metastasis in patients with early non-small cell lung cancer (NSCLC). Methods 200 patients with early NSCLC admitted to our hospital from April 2020 to September 2021 were divided into the control group (100 cases) and observation group (100 cases) by using a computer to randomly generate numbers. The control group received single-port thoracoscopic lobectomy while the observation group patients recceived single-port thoracoscopic segmentectomy. The surgical conditions of the two groups was compared, including lung function [forced vital capacity (FVC), forced expiratory volume in one second (FEV1) and FEV1/FVC ratio], serum trauma response indicators [prostaglandin (PGE2), cortisol (Cor) and C-reactive protein (CRP) ] and tumor cell carcinoembryonic antigen (CEA), cytokeratin 19 fragment (CYFRA21-1) and squamous cell carcinoma antigen (SCC-Ag). The survival and long-term metastasis were tested by Kaplan-Meier curve, and the complications were compared. Results Compared with control group, the intraoperative blood loss in observation group was less and the surgical duration was shorter, but the number of lymph nodes dissection was not significantly different between the two groups. At 3 months after surgery, the lung function parameters FVC, FEV1 and FEV1/FVC in both groups were significantly decreased compared with those before surgery, but the decreases were relatively small in observation group, which had significant differences compared to control group. Serum levels of PGE2, Cor and CRP in the two groups at 1 month after surgery were significantly increased compared with those before surgery, but the increases in observation group were relatively small compared with those in control group. The levels of CEA, CYFRA21-1 and SCC-Ag in both groups was significantly reduced compared to before surgery, and the levels in observation group were lower than those in control group. At 2 years after surgery, metastasis was less in the two groups, and there was no significant difference in disease-free survival between the two groups. In addition, the complications revealed no obvious differences between the two groups. Conclusion Compared with lobectomy, single-port thoracoscopic segmentectomy in the treatment of NSCLC can better relieve the trauma response, maintain the lung function, and reduce the risk of tumor recurrence and metastasis. 2023 Vol. 20 (6): 108-112 [Abstract] ( 186 ) [HTML 1KB] [PDF 1952KB] ( 1246 ) 113 YU Wenxiao, ZHENG Gu, JIN Wangsheng, ZHANG Meng, SHEN Meixiao, WANG Yanjiang The correlation between morphological characteristics of retinal blood vessels and cerebral collateral circulation and prognosis in patients with acute minor stroke Objective To analyze the correlation between the morphological characteristics of retinal blood vessels and the collateral circulation function of the pia mater, and to explore a predictive model for collateral circulation function and prognosis in patients with acute ischemic stroke. Method 53 patients with acute ischemic stroke were included and evaluated for collateral circulation score using head and neck CTA examination. They were divided into good collateral group and bad collateral group; Using the 90 day modified Rankin score as a prognostic indicator. Analyze fundus color photographs and extract morphological parameters of retinal blood vessels, including the main and branch diameters of retinal arteries and veins in each quadrant, curvature, balance, vascular tree classification dimension, geometric dimensions of the optic cup and disc, etc. Analyze the correlation between parameters with significant inter group differences, collateral circulation score, and stroke prognosis score, and establish a regression model for further validation. Result Among the cases included in this study, the good prognosis rate of the good collateral group was significantly higher than that of the bad collateral group. Among the morphological parameters of retinal blood vessels, the central retinal artery equivalent [(43.29±5.02) vs (39.05±4.21), P=0.002] and average arterial diameter [(51.49±5.35) vs (49.07±4.29) , P=0.040)] showed significant differences between groups. There was no significant difference in the other parameters. The central retinal artery equivalent and average arterial diameter were significantly correlated with the pia mater collateral circulation score, with correlation coefficients of 0.418 (P=0.002) and 0.290 (P=0.035) , respectively. There was no significant correlation between other parameters. The logistic regression analysis of collateral circulation score showed that CRAE was correlated with collateral circulation score (OR=1.349, 95% CI 1.101-1.653, P=0.004) , and the area under the ROC curve predicted by CRAE was 0.747 (95% CI 0.615-0.879, P=0.002) ; The mean arterial diameter was not statistically significant in the regression analysis of collateral circulation score (OR=1.086, 95% CI 0.976~1.209, P=0.129) . All retinal vascular parameters were not statistically significant in the 90 day modified Rankin score logistic regression. Conclusion In mild stroke patients, the central retinal artery equivalent is significantly correlated with the pia mater collateral circulation function, which can reflect the state of cerebral collateral circulation function. The morphological parameters of retinal blood vessels tested in this study cannot predict the prognosis of ischemic stroke. 2023 Vol. 20 (6): 113-121 [Abstract] ( 196 ) [HTML 1KB] [PDF 2695KB] ( 1282 ) 122 WANG Jianqiang, CHEN Zeng, CHEN Xiaoqing, LI Yaping, GAN Wei, WANG Fachao Clinical effect of Pingchuan Guben decoction combined with Ai-Chi in the treatment of chronic obstructive pulmonary disease with deficiency of lung and kidney qi and influence of cardiopulmonary function Objective To observe clinical effect of pingchuan guben decoction combined with Ai-Chi in the treatment of chronic obstructive pulmonary disease with deficiency of lung and kidney qi . Methods 80 cases were divided into experimental group and control group, 40 cases in each group. Both groups were given routine drug therapy, nursing measures, guided functional exercise and health education. The control group received Ai-Chi training , and the experimental group received Ping Chuan Guben decoction in addition to the basic treatment of the control group. They were assessed before treatment, at 8 weeks, and 3 months after the end of treatment. Pulmonary function test, main indexes of cardiopulmonary exercise test, scores of St. George Respiratory Questionnaire (SGRQ) and chronic obstructive pulmonary disease Quality of Life Scale (CAT) were recorded. Results Compared with before treatment, FEV1%pred, FEV1, FEV1/FVC, FEV1/VC, AT%pred, VO2max, VO2max/kg, METs, peak RER, Wmax and other indexes in 2 groups were improved for 8 weeks and 3 months after treatment. Compared with the control group after 8 weeks of treatment and 3 months after the end of treatment, the above indexes in the experimental group were significantly improved, and the difference was statistically significant. In this study, there were no adverse events during clinical treatment. Conclusion Pingchuan guben decoction combined with Ai-Chi can improve cardiopulmonary function, exercise endurance, relieve clinical symptoms and improve quality of life in stable COPD patients. 2023 Vol. 20 (6): 122-126 [Abstract] ( 183 ) [HTML 1KB] [PDF 1952KB] ( 1467 ) 127 DONG Zhenghui, LI Zhengang, WANG Yating, QI Jinfang Development and validation of a prediction model of pressure injury in critically ill patients after cardiopulmonary bypass Objective To develop and validate a nomogram model for predicting pressure injury in critically ill patients after cardiopulmonary bypass,which provides a theoretical basis for selecting a pressure injury prediction tool suitable for patients after ICU cardiopulmonary bypass. Methods A total of 268 patients after cardiopulmonary bypass admitted to ICU of a general hospital from January 2021 to December 2021 were eligible for the candidates of this study. General data,treatment parameters,laboratory results and other data were collected from the electric hospital information system. The logsitic regression analysis was performed to detect the risk factors and to develop a nomogram prediction model. The area under receiver operating characteristic curve,calibration curve and decision curve were used to evaluate the prediction ability of the model. Results 1）108 patients developed pressure injury in ICU,the incidence of ICU,acquired pressure injury was approximately 40.29%.2）In logistic regression analysis,vasopressor inotropic score（OR=1.246）,duration of cardiopulmonary bypass（OR=1.015）,APACHEII（OR=1.142）and IL-6（OR=1.003）were identified as risk factors of pressure injury.3）The area under the curve of the model was 0.865,and the sensitivity and specificity were 0.907 and 0.780,respectively. The concordance index of the model was 0.916. Hosmer,lemeshow test：χ2=6.363, P=0.607. The calibration curve showed that it had a good consistency between the observed and actual probability. Conclusion The nomogram model has good predictive efficacy for pressure injury in ICU patients after cardiopulmonary bypass,which is helpful to identify patients with high risk of pressure injury. 2023 Vol. 20 (6): 127-131 [Abstract] ( 177 ) [HTML 1KB] [PDF 2030KB] ( 1152 ) Pharmacy 132 HE Yufang, CHENG Shuting, ZHOU Wangxi Exploration and practice of online pharmaceutical care model for pregnant women based on wechat public platform Objective To build an online pharmaceutical care platform in our hospital, explore the mode of online pharmaceutical care for pregnant women, and analyze the promotion mode and user needs of online pharmaceutical care. Methods With wechat public platform as the carrier, pharmaceutical care was carried out combining online and offline for pregnant women, pharmaceutical science popularization was pushed irregularly, and real-time online pharmaceutical consultation service was provided. A questionnaire survey was conducted to collect user reading and medication consultation data from July 1, 2021 to September 30, 2022, and analyze the age distribution, education level and needs of the public. Results By September 30, 2022, we had accumulated a stable number of 577 users, and promoted more than 30 popular science articles. The questionnaire survey showed that the public platform was well received by the majority of users. Conclusions Wechat public platform provides a good carrier of online pharmaceutical care for pregnant women, allowing them to enjoy professional pharmaceutical care timely, accurately and conveniently. 2023 Vol. 20 (6): 132-138 [Abstract] ( 214 ) [HTML 1KB] [PDF 2641KB] ( 1365 ) Preventive Medicine 139 CHEN Huisu, ZHANG Xingru, SONG Caini, LIU Lihua The current situation and influencing factors of repeated blood donation behavior intention of college students in Changsha Objective To understand the current situation and influencing factors of college students’ repeated blood donation behavior,which is conducive to timely adjustment of the recruitment strategy for college students' voluntary blood donation,and is of great significance to promote the healthy and rapid development of voluntary blood donation. Methods A stratified random sampling method was used to select the undergraduate students from the first to fourth grades of the target university who cooperated with this study as the object of this study. Results A total of 1440 subjects were included in this study,including 843 women,accounting for 58.5% of the total,and 597 men,accounting for 41.5% of the total. The age range was 17-24 years old. The total score of college students’ repeated blood donation behavior intention in Changsha is 120（107,135）. There are differences in each dimension of repeated blood donation behavior intention between college students with and without blood donation experience,and the difference is statistically significant（P<0.001）. Multiple linear regression analysis found that gender,grade,number of good friends,support of different family and friends for blood donation,participation of family and friends in voluntary blood donation,whether to participate in voluntary blood donation,cumulative number of blood donation,and comprehensive experience evaluation of blood donation were the influencing factors of college students' intention to repeat blood donation. Conclusion The intention of repeated blood donation among college students in Changsha city is moderate. It is suggested to strengthen the intention of repeated blood donation among college students in Changsha City from the following perspectives,such as improving blood donation service,optimizing blood donation process,improving blood donation environment and promoting the culture of voluntary blood donation,so as to stabilize the unpaid blood donation team. We will promote the long-term and healthy development of unpaid blood donation. 2023 Vol. 20 (6): 139-144 [Abstract] ( 172 ) [HTML 1KB] [PDF 2369KB] ( 1164 ) Nursing Science 145 LI Fang, ZHU Manli, ZENG Qingling, WANG Ziyan, ZHANG Xilin, QIU Hongxia Psychological Stress Status and Influencing Factors of Unrelated Hematopoietic Stem Cell Donors Objective To investigate the psychological stress of unrelated hematopoietic stem cell donors,explore the relationship between it and social's support and coping style,and analyze its influencing factors. Methods Using the convenient sampling method,94 unrelated donors who underwent hematopoietic stem cell collection in the Department of Hematology of Hunan Provincial People's Hospital from August 2020 to December 2022 were selected as the research objects. The general information questionnaire,Chinese version of Perceived Stress Scale,Perceived Social Support Scale and Simple Coping Style Questionnaire were used to investigate. Independent sample t test and one-way analysis of variance were utilized to determine the difference in psychological stress scores of unrelated donors with different characteristics. Pearson correlation analysis was applied to determine the correlation between psychological stress of unrelated donors and social support,coping style,anxiety and depression. Multiple linear regression analysis was employed to determine the influencing factors of psychological stress of unrelated donors. Results The total score of psychological stress of hematopoietic stem cell unrelated donors was (18.74±8.735),of which 20（21.3%）were health risk psychological stress. The results of univariate analysis showed that there were statistically significant differences in the psychological stress scores of unrelated donors with different ages,education levels,occupational types,permanent residence,and times of blood donation. Correlation analysis showed that the psychological stress score of unrelated donors was negatively correlated with family support,friend support,other support and positive coping style（r=-0.565~0.482,P<0.01）,and positively related to negative coping style. Multiple linear regression analysis showed that positive coping style and friend support were the main factors affecting the psychological stress of unrelated donors（R2=0.373,P<0.001）. Conclusion The psychological stress of hematopoietic stem cell unrelated donors is at a medium level,and more than one-fifth of unrelated donors are health risk psychological stress,which is affected by many factors. Medical staff should actively pay attention to the psychological stress level of unrelated donors,and formulate targeted psychological intervention measures combined with influencing factors to improve the donation willingness and experience of unrelated donors and promote the high-quality development of hematopoietic stem cell donation in China. 2023 Vol. 20 (6): 145-150 [Abstract] ( 167 ) [HTML 1KB] [PDF 1930KB] ( 1450 ) 151 LI Qun, DUAN Anyan, JIANG Jieqiong, LI Ling, HU Jiao, CHEN Wei Prevalence and risk factors of frailty in elderly hypertensive patients in the community: a Meta-analysis Objective To systematically review the prevalence and risk factors for frailty in elderly patients with hypertension. Methods We searched the CNKI, VIP, WanFang Data, SinoMed, PubMed, Web of Science, Embase, and Cochrane database from the inception to February 2023 for the eligible original studies. Studies reporting on the prevalence and risk factors for frailty in elderly hypertensive patients were included. Two reviewers independently screened literature, appraised the study methodological quality, extracted data, then performed meta-analysis using Stata12.0 and RevMan5.4. Results A total of 15 studies including 27 risk factors met the inclusion criteria. The pooled prevalence of frailty in hypertensive patients was 22.5% (95%CI: 16%, 29%). The main risk factors for frailty were age (OR=1.54, 95%CI: 1.34~1.76), females (OR=1.33, 95%CI: 1.21~1.45), hypertension course (OR=25.81, 95%CI: 2.03~327.62), BMI (OR=2.00, 95%CI: 1.28~ 3.12), education level (OR=3.50, 95%CI: 2.26, 5.43), lack of physical activity (OR=1.50, 95%CI: 1.96~ 5.18), depression (OR=1.13, 95%CI: 3.83~17.37), diabetes (OR=1.74, 95%CI: 1.29~2.55), and hearing impairment (OR=1.33, 95%CI: 2.43~4.96) were risk factors for frailty in elderly hypertensive patients. Conclusion Frailty is highly prevalent among hypertensive patients and is affected by many factors. Targeted and individualized interventions should be taken to reduce the incidence of frailty and improve the quality of life of patients. 2023 Vol. 20 (6): 151-156 [Abstract] ( 192 ) [HTML 1KB] [PDF 2195KB] ( 1184 ) 157 LIU Mengqi, HE Pingping, WANG Ping, TIAN Dandan, LIU Yating, ZHANG yingqun Mediating effect of coping style and self-concealment on the relationship between type D personality on social isolation among elderly patients with coronary heart disease Objective To explore the relationship between type D personality, social isolation, coping style and self-concealment among elderly patients with coronary heart disease. Methods A total of 322 elderly patients with coronary heart disease in three grade A tertiary general hospital in Hunan were selected as subjects. Patient were surveyed using the General Alienation Scale (GAS), the Type D personality scale (DS-14), the Medical Coping Modes Questionnaire (MCMQ) and the Self‐Concealment Scale (SCS). Results The sample scored (39.69±11.20) on social isolation, (24.17±9.49) on type D personality, (15.76±5.81) on confrontation coping, (16.02±6.42) on avoidance coping, (10.74±3.81) on submission coping and (34.80±10.26) on self-concealment. There was positively correlation between social isolation, type D personality, avoidance coping, submission coping and self-concealment, while confrontation coping was negatively correlated with any of the other variables. The results of the mediating test showed that the type D personality in elderly patients with coronary heart disease directly and positively predict the social isolation, and indirectly affect separately through confrontation coping, avoidance coping, submission coping as well as self-concealment. Social isolation also could indirectly influenced type D personality through the chain mediating effect of avoidance coping and self-concealment. The total mediating effect accounted for 60.67% of the total effect. Conclusion Social isolation in elderly patients with coronary heart disease was in moderately high levels. Coping style and self-concealment play a partial mediating role between type D personality and social isolation. Medical staffs can improve the level of social isolation in elderly patients with coronary heart disease through the perspectives of changing patients' type D personality, guiding patients to adopt positive coping styles, and reducing patients' self-concealment. 2023 Vol. 20 (6): 157-162 [Abstract] ( 157 ) [HTML 1KB] [PDF 2145KB] ( 1116 ) 163 LI Yilan, PENG Lili, LI Aoqi, XIE Xinwei, WU Fang, LIU Sipeng, CHEN Hongtao Analysis of the current status and influencing factors of family TCM nursing in breast cancer patients after surgery Objective The aim of this study paper is to understand the statues of knowledge,practice and demand of family traditional Chinese medicine nursing for breast cancer patients,and to explore the influencing factors. Methods A total of 352 home patients after breast cancer surgery were investigated by using general data questionnaire and family traditional Chinese medicine nursing knowledge,practice and demand questionnaire. Multiple linear regression was used to analyze the influencing factors of knowledge,practice and demand for family traditional Chinese medicine nursing of breast cancer patients. Results The scores of knowledge,practice and demand of family traditional Chinese medicine nursing were（67.83±7.779）,（30.84±6.942）and（31.02±5.327）,which were in the medium level. Different education level,marital status,family monthly income,time since surgery and postoperative hospital follow-up had statistical differences on the family traditional Chinese medicine nursing attitude awareness of patients. Different age,education level,family monthly income,and postoperative hospital follow-up had statistical differences on the family traditional Chinese medicine nursing behavior of patients. Different age,marital status,implementation of traditional Chinese medicine nursing and monthly family income had statistical differences on the needs of traditional Chinese medicine family nursing. Conclusion Breast cancer patients have higher cognition level and need for family traditional Chinese medicine nursing,but their traditional Chinese medicine nursing behavior needs to be further improved. Hospitals and communities should actively carry out family traditional Chinese medicine nursing health education,promote traditional family traditional Chinese medicine nursing health care technology,and strive to improve patients’ cognitive level,enhance practical ability,and meet patients’ needs at home. 2023 Vol. 20 (6): 163-170 [Abstract] ( 181 ) [HTML 1KB] [PDF 2097KB] ( 1089 ) 171 ZHOU Lihuan, XU Jingcan, HAN Huiwu, DAI Weiwei, PENG Min, ZHAO Nan, LI Xinyi, LUO Wenjing, YU Ling, ZHOU Qiuhong The intervention effect of ESM Cluster Nursing Model in Type 2 Diabetes Inpatients with High Risk Factors of Diabetic Foot Objective To explore the effect of ESM cluster nursing mode on foot nursing knowledge, behavior, foot skin condition, anxiety and depression in hospitalized type 2 diabetes high-risk foot patients. Methods From September to December 2022, a total of 88 cases of high-risk foot patients with type 2 diabetes in the Department of Endocrinology of a Grade A tertiary hospital in Changsha, Hunan Province were selected as the study objects by convenient sampling method, and were divided into control group and intervention group with 44 cases in each group. The control group received routine care, and the experimental group received ESM cluster care mode on this basis, and the intervention time was 1 month. The patients' foot care knowledge, foot care behavior, anxiety and depression, and foot skin condition were evaluated and investigated before and after the intervention. Results The improvement of foot care knowledge, foot care behavior and anxiety and depression in the intervention group was better than that in the control group, and the foot skin condition was significantly improved, with statistical significance. Conclusion The ESM cluster nursing mode can effectively improve foot nursing knowledge, promote foot nursing behavior, reduce foot skin abnormality rate, and improve anxiety and depression of patients with type 2 diabetes. 2023 Vol. 20 (6): 171-176 [Abstract] ( 177 ) [HTML 1KB] [PDF 2205KB] ( 1438 )

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