SIRT1/PGC-1α通路在断指再植大鼠皮瓣成活及皮瓣自噬中的作用机制

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Abstract Objective To explore the role and possible mechanism of silencing information regulatory factor 1(SIRT1) /peroxisome proliferator-activated receptor-γ-coactivator 1α (PGC-1α) signaling pathway in replanted flaps survival and autophagy in rat finger replantation. Methods According to the experimental design, 50 SD rats were randomly divided into control group, SIRT1 agonist (SRT-1460) group, rapamycin (RAPA) group, RAPA+SIRT1 inhibitor (EX527) group and SRT-1460+RAPA group, with 10 rats in each group. The survival rate and the survival area of the skin flap were observed in each group. The expression levels of SIRT1, PGC-1α, Beclin1, microtubule-associated protein 1 light chain 3II (LC3II) in the skin flap of the rats in each group were detected by Western blotting. The mRNA expression levels of SIRT1, PGC-1α, Beclin1 and LC3II in rat skin flap were detected by RT-qPCR. Results Compared with the control group, the survival rate (74.81±4.38) and the survival area (6.22±1.15) of the rat skin flap in SRT-1460 group were significantly increased, the protein and mRNA expressions of SIRT1, PGC-1α, Beclin1, LC3II were significantly increased in rat skin flap after severed finger replantation, the survival rate (76.50±5.82) and the survival area (6.94±2.61) were increased in RAPA group, the protein and mRNA expressions of SIRT1, PGC-1α, Beclin1, LC3II were significantly increased in rat skin flap after severed finger replantation. Compared with RAPA group, the survival rate (67.23±6.28) and the survival area (5.91±1.83) of the rat skin flap in RAPA+EX527 group were decreased, the protein and mRNA expressions of SIRT1, PGC-1α, Beclin1, LC3II were significantly decreased. Compared with SRT-1460 group, the survival rate (89.71±7.13) and the survival area (8.32±1.26) of rat skin flap in SRT-1460+RAPA group were decreased, the protein and mRNA expressions of SIRT1, PGC-1α, Beclin1, LC3II were significantly decreased. Conclusion SIRT1/PGC-1α signaling pathway plays an important role in the survival of rat skin flap after finger replantation. Inhibition of this pathway can reduce autophagy and the survival of rat skin flap after finger replantation. Activation of this pathway can enhance autophagy and improve the survival of rat skin flap after finger replantation.

Key words： finger replantation replanted flaps SIRT1/PGC-1α pathway autophagy

Received: 26 August 2023

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R658.1

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	CHEN Jiaofeng
	CHEN Shengyuan
	YU Jing

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CHEN Jiaofeng,CHEN Shengyuan,YU Jing. The mechanism of SIRT1/PGC-1α pathway on skin flap survival and autophagy in rat severed finger replantation[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(6): 44-48.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2023/V20/I6/44

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