目的: 研究一种高效可靠的脱细胞真皮基质的制备方法。方法: 以猪皮为实验材料,用磷脂酶A2与脱氧胆酸钠组合配制脱细胞液,用DNase溶液进行洗涤,对猪皮进行脱细胞处理。对所制备脱细胞真皮基质进行DAPI、HE及免疫组化染色鉴定,并对其胶原等成分进行定量检测。结果: 所制备基质脱细胞效果良好,胶原等主要成分含量保留较高。结论: 用磷脂酶A2与脱氧胆酸钠组合的脱细胞方法用时短,简单高效,符合脱细胞要求,制成的真皮基质结构及成分保留完整,是一种理想的生物医学材料。
Abstract
Objective To investigate an efficient and reliable method for the preparation of acellular dermal matrix. Methods Porcine skin was selected as the experimental material. Decellularization was performed using a decellularization solution composed of phospholipase A2 and sodium deoxycholate, followed by washing with a 3.4M NaCl high-salt solution and DNase solution. The prepared acellular dermal matrix was characterised using DAPI staining, hematoxylin and eosin (H&E) staining, and immunohistochemical analysis. Additionally, the quantitative assessment of collagen and other components was conducted. Results The prepared matrix demonstrated effective decellularization, exhibiting a high retention of major components such as collagen. Conclusion The decellularization method that employs a combination of phospholipase A2 and sodium deoxycholate is characterised by its efficiency, simplicity, and effectiveness. It fulfils the requirements for decellularization and is a time-efficient process. The resulting dermal matrix exhibits structural integrity and biochemical components, rendering it an ideal biomedical material.
关键词
脱细胞 /
脱细胞真皮基质 /
生物医学材料
Key words
decellularization /
decellularized dermal matrix /
biomedical material
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基金
湖南师范大学医学院2023年度开放基金（KF2022017）;湖南师范大学2023年大型仪器测试开放基金资助项目（23CSY170）
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