DEHP暴露对斑马鱼胚胎心脏发育的影响

Krishna Baral; 史洪北晨; 张阳; 陈橙; 孟阳; 赵明一

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湖南师范大学学报医学版 ›› 2025, Vol. 22 ›› Issue (1) : 16-22.

基础医学

DEHP暴露对斑马鱼胚胎心脏发育的影响

Krishna Baral, 史洪北晨, 张阳, 陈橙, 孟阳, 赵明一

作者信息 +

Effects of DEHP exposure on heart development of zebrafish embryos

Krishna Baral, SHI Hongbeichen, ZHANG Yang, CHEN Cheng, MENG Yang, ZHAO Mingyi

Author information +

文章历史 +

摘要

目的：邻苯二甲酸二（2-乙基己基）酯（Di-2-ethylhexyl phthalate,DEHP）是一种广泛应用的增塑剂,已知对人类健康产生不良影响。先前的研究集中于DEHP对心血管系统的影响,但其对早期胚胎心脏发育的影响尚未阐明。此研究创新性地观察 DEHP诱导斑马鱼幼鱼心脏发育异常情况,探讨了DEHP如何通过激活氧化应激途径影响斑马鱼胚胎心脏的发育。方法：本研究采用5hpf的斑马鱼胚胎为研究对象,设置对照组、DEPH处理组。记录并计算斑马鱼存活率、孵化率、心率、畸形率;通过心脏转基因斑马鱼cmcl2：EGFP观察心脏发育状态;荧光探针2, 7-二氯荧光黄双乙酸盐法检测斑马鱼活性氧（reactive oxygen species,ROS）含量;吖啶橙染色检测心脏部位凋亡水平。结果：与对照组相比,DEHP暴露组斑马鱼胚胎存活率及孵化率均降低,但无统计学意义;心率显著增加（P<0.0001）;心脏结构改变,静脉窦（sinus venosus,SV）和动脉球（bulbus arteriosus,BA）间距增加（P<0.05）、心包面积增大（P<0.0001）;活性氧（ROS）表达水平上升（P<0.01）,结果均具有统计学意义。结论：DEHP暴露的斑马鱼幼鱼心率增快、心脏结构异常、心脏ROS表达水平下降。DEHP可能通过激活ROS氧化应激系统诱导斑马鱼心脏发育异常。

Abstract

Objective Di-2-ethylhexyl phthalate (DEHP) is a widely used plasticizer, which is known to have adverse effects on human health. Although its impact on cardiovascular health has been extensively studied, the specific effects on early embryonic heart development remain unclear. This study innovatively observed the abnormal heart development of zebrafish larvae induced by DEHP, and discussed how DEHP affected the development of zebrafish embryonic heart by activating oxidative stress pathway. Methods Utilizing zebrafish embryos at 5 hours post fertilization (hpf), we established control and DEHP-treated groups. We recorded and analyzed survival rate, hatching rate, heart rate, and malformation rate. Heart development was monitored using a cardiac-specific transgenic zebrafish line (cmcl2: EGFP), and oxidative stress was assessed by measuring reactive oxygen species (ROS) with a fluorescent probe 2, 7-dichlorofluorescein diacetate. Apoptosis in heart cells was detected via acridine orange staining. Results Compared with the control group, the survival rate and hatching rate of zebrafish embryos in the DEHP exposure group decreased (P>0.05), but there was no statistical significance. Heart rate increased significantly (P<0.0001); the distance between sinus venosus (SV) and bulbus arteriosus (BA) increased (P<0.05), and the area of pericardium increased (P<0.0001). The expression level of reactive oxygen species (ROS) increased (P<0.01), and the results were statistically significant. Conclusion The heart rate of zebrafish larvae exposed to DEHP increased, the heart structure was abnormal, and the expression level of cardiac ROS decreased. DEHP may induce abnormal heart development in zebrafish by activating the ROS oxidative stress system.

导出引用

Krishna Baral, 史洪北晨, 张阳, 陈橙, 孟阳, 赵明一. DEHP暴露对斑马鱼胚胎心脏发育的影响[J]. 湖南师范大学学报医学版. 2025, 22(1): 16-22

Krishna Baral, SHI Hongbeichen, ZHANG Yang, CHEN Cheng, MENG Yang, ZHAO Mingyi. Effects of DEHP exposure on heart development of zebrafish embryos[J]. Journal of Hunan Normal University(Medical Science). 2025, 22(1): 16-22

中图分类号： R725.4

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