目的：基于微小RNA（miRNAs）分子标志物验证其在黑色素瘤的诊断价值及预后评估。方法：选取2020年1月—2023年5月江苏省盐城市第一人民医院收治的92例黑色素瘤患者作为恶性组,按照1∶1匹配原则选取同期、同年龄段92例皮肤良性病变患者作为对照组。分析两组血清miRNAs表达水平,比较不同病理特征患者血清miR-204-5p、miR-126-3p、miR-193b表达水平,受试者工作特征曲线（ROC）、曲线下面积（AUC）分析miR-204-5p、miR-126-3p、miR-193b表达水平与病理特征的相关性及对黑色素瘤诊断价值,比较不同预后患者miR-204-5p、miR-126-3p、miR-193b表达水平,对比不同miR-204-5p、miR-126-3p、miR-193b表达水平患者1年生存率。结果：恶性组miR-204-5p、miR-126-3p、miR-193b表达水平均低于对照组;不同临床分期、肿瘤侵袭性、淋巴结转移患者血清miR-204-5p、miR-126-3p、miR-193b表达水平比较,差异具有统计学意义;miR-204-5p、miR-126-3p、miR-193b与临床分期、肿瘤侵袭性、淋巴结转移呈负相关;miR-204-5p、miR-126-3p、miR-193联合的AUC最大,为0.907,明显大于各指标单独诊断;预后良好患者血清miR-204-5p、miR-126-3p、miR-193b高于预后不良患者;低水平miR-204-5p、miR-126-3p、miR-193b患者1年生存率低于高水平miR-204-5p、miR-126-3p、miR-193b。结论：黑色素瘤患者血清miR-204-5p、miR-126-3p、miR-193b与临床分期、肿瘤侵袭性、淋巴结转移及预后密切相关,miR-204-5p、miR-126-3p、miR-193b联合有助于提高黑色素瘤诊断价值。
Abstract
Objective To validate the diagnostic value and prognostic assessment of melanoma based on microRNAs (miRNAs) molecular markers. Methods A total of 92 melanoma patients admitted to the First People's Hospital of Yancheng City, Jiangsu Province from January 2020 to May 2023 were selected as the malignant group, and 92 patients with benign skin lesions in the same period and age group were selected as the control group according to the 1:1 matching principle. The expression levels of serum miRNAs were analyzed in the two groups, and the expression levels of serum miR-204-5p, miR-126-3p, and miR-193b were compared in patients with different pathological features. The correlation between the expression levels of miR-204-5p, miR-126-3p, and miR-193b and pathological features and their diagnostic value for melanoma were analyzed using the receiver operating characteristic (ROC) and area under the curve (AUC). The expression levels of miR-204-5p, miR-126-3p and miR-193b in patients with different prognosis were compared, and the 1-year survival rate of patients with different expression levels of miR-204-5p, miR-126-3p and miR-193b was compared. Results The expression levels of miR-204-5p, miR-126-3p and miR-193b in malignant group were lower than those in control group. There were statistically significant differences in the expression levels of miR-204-5p, miR-126-3p and miR-193b in patients with different clinical stages, tumor aggressiveness and lymph node metastasis. miR-204-5p, miR-126-3p and miR-193b were negatively correlated with clinical stage, tumor invasiveness and lymph node metastasis. The AUC of the combination of miR-204-5p, miR-126-3p and miR-193 was the largest (0.907), which was significantly higher than that of the single diagnosis of each index. Serum miR-204-5p, miR-126-3p and miR-193b in patients with good prognosis were higher than those in patients with poor prognosis. The 1-year survival rate of patients with low levels of miR-204-5p, miR-126-3p and miR-193b was lower than that of patients with high levels of miR-204-5p, miR-126-3p and miR-193b. Conclusion Serum miR-204-5p, miR-126-3p and miR-193b of melanoma patients are closely related to clinical stage, tumor invasiveness, lymph node metastasis and prognosis, and the combination of miR-204-5p, miR-126-3p and miR-193b can improve the diagnostic value of melanoma.
关键词
微小RNA /
分子标志物 /
黑色素瘤 /
诊断 /
预后 /
病理特征
Key words
miRNAs /
molecular markers /
melanoma /
diagnosis /
prognosis /
pathological feature
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参考文献
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基金
徐州医科大学附属医院发展基金资助项目（XYFM202303）; 江苏省优势学科建设工程项目（YSHL2001-1141）
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