目的：评价丹参叶提取物的食用安全性及抗氧化功能,为其药食领域的研发和推广应用提供依据。方法：采用急性毒性、Ames、哺乳动物红细胞微核试验、体外哺乳类细胞染色体畸变试验和大鼠90 d经口毒性试验对丹参叶提取物的安全性进行评价,并在90 d经口毒性试验结束时,检测大鼠血清和肝组织中超氧化物歧化酶（superoxide dismutase,SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-Px）的活性和丙二醛（malondialdehyde,MDA）含量。结果：丹参叶提取物对雌、雄性大鼠经口LD50均大于10 g/kg体质量,属无毒级,三项遗传毒性试验结果均为阴性,大鼠90 d喂养实验各项结果（动物一般观察;体重、摄食量及食物利用率;血液学及血液生化学检测结果;大鼠尿常规、大体解剖及组织病理学影响）均未见明显毒性。低、中、高剂量组雌、雄大鼠血清和肝组织SOD、GSH-Px水平均显著高于对照组,MDA水平均显著低于对照组。结论：丹参叶提取物在本实验条件下为无毒物,未显示有急性、亚慢性毒性及遗传毒性;血清和肝组织的氧化应激指标检测结果提示,丹参叶提取物具有一定的抗氧化功能。
Abstract
Objective The aim of this study was to evaluate the food safety and antioxidant function of S. miltiorrhiza leaf extracts, so as to provide a basis for its research and development and popularization in the field of medicinal and food applications. Methods The safety of S. miltiorr0hiza leaf extract was evaluated using acute toxicity, Ames, mammalian erythrocyte micronucleus test, in vitro mammalian cell chromosome aberration test and 90-day oral toxicity test in rats, and at the end of the 90-day oral toxicity test, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the serum and liver tissues of rats and the malondialdehyde (MDA) content. Results The oral LD50 of S. miltiorrhiza leaf extract in female and male rats was more than 10 g/kg, which was non-toxic, and the results of three genotoxicity tests were negative. The results of the 90-day feeding experiment in rats (general observation; body weight, food intake, and food utilization; hematology and blood biochemistry; urine, gross anatomy, and histopathological effects in rats) did not show any significant toxicity. The levels of SOD and GSH-Px in serum and liver tissues of female and male rats in the low-dose, medium-dose and high-dose groups were significantly higher than those in the control group. The levels of MDA in the group were significantly lower than those in the control group. Conclusion S. miltiorrhiza leaf extract was non-toxic under the experimental conditions, and did not show acute, sub-chronic toxicity and genotoxicity; the results of oxidative stress indexes in serum and liver tissues suggested that S. miltiorrhiza leaf extract had certain antioxidant functions.
关键词
丹参叶提取物 /
抗氧化 /
急性毒性 /
遗传毒性 /
亚慢性毒性
Key words
Salvia miltiorrhiza leaf extract /
antioxidant /
acute toxicity /
genotoxicity /
subchronic toxicity
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基金
湖南省自然科学基金医卫联合基金“Cd2+通过调控小鼠卵巢颗粒细胞中COX2/PGE2/PTGER3干扰雌激素合成的研究”（2025JJ80778）; 湖南省卫健委项目“白介素11及其受体介导JAK/STAT信号通路在石英粉尘致肺炎性反应及纤维化中的作用及机制”（D202312046027）
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