目的 研究毛冬青提取物外用对放射性口腔黏膜炎模型小鼠创面愈合的影响,并初步探讨其可能的作用机制。方法 选取8周龄雄性 C3H小鼠,通过X 射线单次照射法照射口腔及舌部诱导复制小鼠放射性口腔黏膜炎（RTOM）模型,随机分为模型组、细胞生长因子组和冬青组。HE染色观察黏膜病损恢复情况、成纤维细胞密度情况。免疫组化评价炎性因子TNF-α和IL-6的表达情况,Western blot进一步检测各组TNF-α和IL-6蛋白的表达。结果 成功复制小鼠RTOM模型。模型组小鼠7d后表现为口腔溃疡溃疡III级,口腔黏膜上皮层厚度变薄,角化上皮结构轻微紊乱,毛细血管扩张,可见炎性细胞浸润;细胞生长因子组小鼠表现为口腔溃疡溃疡II级,颊黏膜上皮厚度较正常组略微增加,角化上皮结构正常;冬青组小鼠处理7 d后表现为口腔溃疡溃疡I级,颊黏膜角化增厚,角化上皮结构正常。免疫组化和Western blot结果显示,细胞生长因子组和冬青组小鼠颊黏膜TNF-α及IL-6表达明显低于模型组,冬青组小鼠颊黏膜TNF-α及IL-6表达明显低于细胞生长因子组,细胞生长因子组和冬青组小鼠TNF-α及IL-6表达阳性率明显低于模型组,冬青组小鼠TNF-α及IL-6表达阳性率明显低于细胞生长因子组。结论 毛冬青提取物外用对小鼠RTOM的溃疡创面有修复作用。

Objective To investigate the effect of Ilex pubescens extract on the wound healing effect of mice with radiation oral mucositis and to explore its possible mechanism. Methods Male C3H mice with 8 weeks old were selected to reproduce the model of radiation oral mucositis（RTOM）in mice by X-ray irradiation of the oral and tongue parts. They were randomly divided into model group,positive control and experimental groups. HE staining was used to observe the recovery of mucosal lesions and the density of fibroblasts. The expression of IL-6 and TNF-α protein was detected by immunohistochemistry. Results The mouse RTOM model was successfully replicated. After 7 days,the model group mice showed grade III oral ulcer,thinning of the oral mucosal epithelial layer,slight disorder of keratinized epithelial structure,dilation of capillaries,and visible infiltration of inflammatory cells;The mice in the cell growth factor group exhibited grade II oral ulcer,slightly increased thickness of buccal mucosal epithelium compared to the normal group,and normal keratinized epithelial structure;After 7 days of treatment,the mice in the Ilex exract group showed grade I oral ulcer,increased keratinization of the buccal mucosa,and normal keratinized epithelial structure. Immunohistochemical and Western blot results showed that the expression of TNF - and IL-6 in the cheek mucosa of mice in the cell growth factor group and Ilex exract group was significantly lower than that in the model group. The expression of TNF - and IL-6 in the cheek mucosa of mice in the Ilex exract group was significantly lower than that in the cell growth factor group. The positive rates of TNF - and IL-6 expression in the cell growth factor group and Ilex exract group were significantly lower than those in the model group. The positive rates of TNF - and IL-6 expression in the Ilex exract group were significantly lower than those in the cell growth factor group. Conclusion Extracts of Ilex have a repairing effect on ulcer wounds in mice with RTOM.