抗菌肽在食源性致病菌检测中的研究进展

李湘; 郑希; 郭玉兰; 徐坤

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湖南师范大学学报医学版 ›› 2023, Vol. 20 ›› Issue (6) : 1-8.

特邀综述

抗菌肽在食源性致病菌检测中的研究进展

李湘^1,2,3,4#, 郑希^1,2,3,4#, 郭玉兰⁵, 徐坤^1,2,3,4

作者信息 +

Research progress on the application of antimicrobial peptides in the detection of foodborne pathogenic bacteria

LI Xiang^1,2,3,4, ZHENG Xi^1,2,3,4, GUO Yulan⁵, XU Kun^1,2,3,4

Author information +

文章历史 +

摘要

由致病菌引起的食源性疾病严重威胁着人类的健康,是较为严重的公共卫生问题。快速、准确检测食品中的食源性致病菌对于防控食源性疾病具有重要意义。近年来,人们开发了多种不同的高灵敏度和特异性的快速检测方法,以期实现对食品中的病原菌的快速检测,这些方法均需要从复杂的食品基质中对细菌进行特异性的识别。为了实现这一目标,很多小分子物质被开发出来,如抗体、适配体、噬菌体、抗菌肽（antimicrobial peptides,AMPs）、凝集素、酶等。这些小分子物质在应用中存在着不同的优缺点,其中抗菌肽的应用越来越受到人们的重视,因为其稳定性高、合成简单、容易获得、对细菌的亲和力高,与抗体、DNA探针等相比,抗菌肽成本低,在恶劣环境中更稳定,易于大规模生产,因此被应用到多种快速检测方法中。本文系统总结了AMPs在食源性致病菌中的检测应用,并探讨了其应用前景。

Abstract

Foodborne diseases caused by pathogenic bacteria are a serious threat to human health and a serious public health problem. Rapid and accurate detection of foodborne pathogenic bacteria in food is important for the prevention and control of foodborne diseases. In recent years, a variety of different highly sensitive and specific rapid detection methods have been developed with the aim of realizing the rapid detection of pathogenic bacteria in food. These methods all need to specifically identify bacteria from complex food matrixes. To achieve this goal, many small molecule substances have been developed, such as antibodies, aptamers, phages, antimicrobial peptides (AMPs), lectins, and enzymes. These small molecule substances have different advantages and disadvantages in application. The application of antimicrobial peptides has received more and more attention because of their high stability, simple synthesis, easy availability, high affinity for bacteria, low cost of AMPs compared with antibodies, DNA probes, more stable in harsh environments, and easy to be mass-produced. Therefore, they are being applied to the development and utilization of a variety of rapid detection methods. In this review, we systematically summarized the application of antimicrobial peptides in the detection of foodborne pathogens, and discussed its application prospects.

导出引用

李湘, 郑希, 郭玉兰, 徐坤. 抗菌肽在食源性致病菌检测中的研究进展[J]. 湖南师范大学学报医学版. 2023, 20(6): 1-8

LI Xiang, ZHENG Xi, GUO Yulan, XU Kun. Research progress on the application of antimicrobial peptides in the detection of foodborne pathogenic bacteria[J]. Journal of Hunan Normal University(Medical Science). 2023, 20(6): 1-8

中图分类号： R155

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基金

国家自然科学基金面上项目“食品中多种病原菌的‘信号转导-级联放大’检测体系的构建”（82173572）; 湖南省自然科学基金面上项目“基于 DNAzyme 和 HCR 构建比色/荧光双信号大肠杆菌生物传感器”（2023JJ30430）,“ 双胍新衍生物SMK-002阻断PKM2入核下调脂质合成抑制EMT阻止膀胱癌转移的作用和机制研究”（82172653）