目的：探讨miR-552在结直肠癌组织中表达及其与临床病理特征的关系。方法：选取2020年1月―2022年12月在我院检查的147例结直肠癌患者为研究对象,比较结直肠癌患者肿瘤组织、癌旁组织miR-552水平。根据miR-552表达情况将受试者分为高表达组（miR-552表达量≥1.28）及低表达组（miR-552表达量<1.28）,分析影响miR-552表达的危险因素,进一步探讨miR-552与病理特征相关性。结果：结直肠癌患者肿瘤组织miR-552水平为（9.86±1.22）,高于癌旁组织的（3.11±0.24）,差异有统计学意义;miR-552高表达组TNM分期处于Ⅲ~Ⅳ、肿瘤病灶直径≥5 cm、浸润深度为浆膜层、发生淋巴结转移的比例均高于miR-552低表达组,差异有统计学意义;经Logistic回归分析发现TNM分期Ⅲ~Ⅳ期、肿瘤病灶直径≥5 cm、浸润浆膜层、淋巴结转移是导致miR-552呈现高表达的危险因素;TNM分期、肿瘤病灶直径、浸润深度、淋巴结转移与miR-552表达呈现正相关。结论：结直肠癌组织中miR-552表达水平高于癌旁组织,且TNM分期、肿瘤病灶直径、浸润浆膜层及淋巴结转移为miR-552表达水平的影响因素,并与miR-552表达水平具有显著相关性。
Abstract
Objective To investigate the expression of miR-552 in colorectal cancer tissues and its relationship with clinicopathological characteristics. Methods 147 patients with colorectal cancer who were examined in our hospital from January 2020 to December 2022 were selected as the study subjects. The levels of miR-552 in tumor tissues and adjacent normal tissues were compared. According to the expression of miR-552, the subjects were divided into high expression group (miR-552 expression level was 1.28 or higher) and low expression group (miR-552 expression level was lower than 1.28). The risk factors affecting miR-552 expression were analyzed, and the correlation between miR-552 and pathological characteristics was discussed. Results The level of miR-552 in tumor tissue of patients with colorectal cancer (9.86±1.22) was higher than that in adjacent normal tissues. The proportions of TNM stage III and IV, tumor diameter ≥5 cm, serosal layer infiltration and lymph node metastasis in the high expression group were higher than those in the low expression group. Logistic regression analysis showed that TNM stage III and IV, tumor diameter ≥ 5cm, serosal layer infiltration and lymph node metastasis were independent influencing factors of high miR-552 expression. TNM stage, tumor diameter, invasion depth and lymph node metastasis were positively correlated with miR-552 expression. Conclusion The expression level of miR-552 in colorectal cancer tissue is higher than that in adjacent tissues, and TNM stage, tumor diameter, serosal layer infiltration, and lymph node metastasis are influencing factors for miR-552 expression level, which is significantly correlated with miR-552 expression level. .
关键词
miR-552 /
结直肠癌 /
临床病理特征
Key words
miR-552 /
colon cancer /
clinicopathological characteristic
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] 邹慧敏, 张莉莉, 蔡懿婷, 等. 转移相关基因1在结直肠癌组织中的表达及其与临床病理特征和预后的相关性[J]. 现代肿瘤医学, 2019, 027(21) : 3839-3842.
[2] Chen L, Heikkinen L, Wang C, et al.Trends in the development of miRNA bioinformatics tools[J]. Brief Bioinform, 2019, 20(5) : 1836-1852.
[3] 李猛, 仲雅婷, 陈成, 等. miR-552和miR-592在胃癌组织中的表达及其与临床病理特征的关系[J]. 胃肠病学和肝病学杂志, 2021, 30 (10) : 1096-1100+1106.
[4] 张政, 张澍田, 李鹏.2018年美国癌症协会结直肠癌筛查指南解读[J]. 中国实用内科杂志, 2018, 38(9) : 814-816.
[5] 洪嵩, 蔡东峰, 张靖, 等. miR-552靶向调控Wnt抑制因子-1促进MG-63骨肉瘤细胞增殖、侵袭、迁移的实验研究[J]. 临床肿瘤学杂志, 2019, 24(6) : 494-499.
[6] 杨祎, 何颖华, 贾菲, 等. 双歧杆菌三联活菌胶囊联合FOLFOX方案用于结直肠癌术后对肠道微生态及抗肿瘤免疫应答的影响[J]. 湖南师范大学学报 (医学版) , 2022, 19(1) : 95-98.
[7] Li J, Ma X, Chakravarti D, et al.Genetic and biological hallmarks of colorectal cancer[J]. Genes Dev, 2021, 35(11-12) : 787-820.
[8] Patel SG, Karlitz JJ, Yen T, et al.The rising tide of early-onset colorectal cancer: a comprehensive review of epidemiology, clinical features, biology, risk factors, prevention, and early detection[J]. Lancet Gastroenterol Hepatol, 2022, 7(3) : 262-274.
[9] 王璐, 孙小虎, 白静慧, 等. miR-584下调CCN2对结直肠癌SW1116细胞增殖、侵袭的影响及机制研究[J]. 疑难病杂志, 2022, 21(1) : 17-23.
[10] Wang H.MicroRNAs and Apoptosis in Colorectal Cancer[J]. Int J Mol Sci, 2020, 21(15) : 5353.
[11] Wang L, Cho KB, Li Y, et al.Long Noncoding RNA (lncRNA) -Mediated Competing Endogenous RNA Networks Provide Novel Potential Biomarkers and Therapeutic Targets for Colorectal Cancer[J]. Int J Mol Sci, 2019, 20(22) : 5758.
[12] Das PK, Islam F, Lam AK.The Roles of Cancer Stem Cells and Therapy Resistance in Colorectal Carcinoma[J]. Cells, 2020, 9(6) : 1392.
[13] 覃先蓬, 赵高平, 杨春, 等. 沉默circ_0000745通过靶向miR-296-5p对结直肠癌细胞增殖及转移的影响[J]. 华中科技大学学报 (医学版) , 2022, 51(1) : 31-37.
[14] 刘昌化, 罗康宁, 闫圣玉, 等. miR-515-5p通过靶向TRIM31调控PI3K/AKT信号通路对结直肠癌细胞增殖、侵袭及迁移的影响[J]. 医学研究生学报, 2022, 35(5) : 478-484.
[15] Zhang N, Hu X, Du Y, et al.The role of miRNAs in colorectal cancer progression and chemoradiotherapy[J]. Biomed Pharmacother, 2021, 134(1) : 111099.
[16] Sendi H, Yazdimamaghani M, Hu M, et al.Nanoparticle Delivery of miR-122 Inhibits Colorectal Cancer Liver Metastasis[J]. Cancer Res, 2022, 82(1) : 105-113.
基金
四川省科技创新创业项目（编号：20MZGC0036）
PDF(1905 KB)
