芹菜素通过下调HK-2表达抑制非小细胞肺癌H460细胞糖酵解和球形成

孙菲; 袁庆; 张坚松

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湖南师范大学学报医学版 ›› 2023, Vol. 20 ›› Issue (1) : 1-5.

基础医学

芹菜素通过下调HK-2表达抑制非小细胞肺癌H460细胞糖酵解和球形成

孙菲, 袁庆, 张坚松

作者信息 +

Apigenin Inhibits Glycolysis and Spheroid Formation via Downregulation of HK-2 Expression in Non-Small Cell Lung Cancer H460 Cells

SUN Fei, YUAN Qing, ZHANG Jiansong

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文章历史 +

摘要

目的: 研究芹菜素能否通过下调糖代谢相关激酶HK-2表达抑制体外培养非小细胞肺癌H460细胞糖酵解及自我更新能力。方法: HK-2 cDNA转染、亚细胞毒浓度的芹菜素（20μM、40μM和80μM）处理或芹菜素（80μM）联合HK-2 cDNA转染H460细胞。蛋白质印迹检测HK-2表达水平,检测乳酸产物和葡萄糖消耗水平,同时,球形成实验测验自我更新能力。结果: 亚细胞毒浓度的芹菜素以剂量依赖趋势下调非小细胞肺癌H460细胞的糖代谢相关激酶HK-2表达和减少乳酸产物和葡萄糖消耗,并且降低球形成率。此外,过表达HK-2能救援芹菜素抑制非小细胞肺癌H460细胞乳酸产生、葡萄糖消耗以及球形成率作用。结论: 芹菜素通过下调HK-2表达抑制非小细胞肺癌H460细胞糖酵解及自我更新能力。

Abstract

Objective To investigate whether apigenin (API) inhibits the glycolysis and self-renewal capacity by downregulating the expression of glucose metabolism related kinase HK-2 in non-small cell lung cancer (NSCLC) H460 cells in vitro. Methods H460 cells were transfected with HK-2 cDNA, treated with sub-cytotoxicity concentrations of API (20 μM; 40 μM and 80 μM) or in combination with API (80 μM) treatment and HK-2 cDNA transfection. Western blot was utilized to detect the expression of HK-2. The levels of lactate production and glucose consumption were using measurement of lactate production and glucose consumption assays. The spheroid formation assay was employed to detect the self-renewal capacity in H460 cells. Results The sub-cytotoxic concentrations of API decreased the expression of glucose metabolism related kinase HK-2 and reduced lactiate production and glucose consumption, as well as decreased sphere formation rate in NSCLC H460 cells in a dose-dependent tendency. Moreover, overexpression of HK-2 could rescue the inhibitory effects of API on lactate production, glucose consumption and spheroid formation in NSCLC H460 cells. Conclusion API can inhibit glycolysis and self-renewal capacity by downregulating HK-2 expression in NSCLC H460 cells.

导出引用

孙菲, 袁庆, 张坚松. 芹菜素通过下调HK-2表达抑制非小细胞肺癌H460细胞糖酵解和球形成[J]. 湖南师范大学学报医学版. 2023, 20(1): 1-5

SUN Fei, YUAN Qing, ZHANG Jiansong. Apigenin Inhibits Glycolysis and Spheroid Formation via Downregulation of HK-2 Expression in Non-Small Cell Lung Cancer H460 Cells[J]. Journal of Hunan Normal University(Medical Science). 2023, 20(1): 1-5

中图分类号： R734.2

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