HSP70基因对胆管癌细胞增殖、侵袭及迁移的影响

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摘要 目的: 研究HSP70基因对胆管癌细胞增殖、侵袭及迁移的影响。方法: qPCR及Western blot方法检测胆管癌组织和细胞以及正常胆管组织和细胞中HSP70基因的表达差异情况。利用RNA干扰技术沉默抑制人胆管癌细胞HuCC-T1中HSP70基因表达,然后分别利用CCK-8实验、Transwell实验检测HSP70基因表达下调对HuCC-T1细胞增殖、侵袭及迁移能力的影响。结果: HSP70基因在胆管癌组织和细胞中异常高表达。在HuCC-T1细胞中抑制HSP70基因表达后,HuCC-T1细胞的增殖、侵袭及迁移能力显著下降。结论: HSP70基因在胆管癌细胞中可能起着促进肿瘤发生、发展的作用,可以将其作为胆管癌基因治疗的靶点进行更深入的研究。

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	张豹
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	舒升光

关键词 ：热休克蛋白70, 胆管癌, 增殖, 侵袭, 迁移

Abstract：Objective To study the effect of HSP70 gene on proliferation, invasion and migration of bile duct cancer cells. Methods qPCR and Western blot were used to detect the difference of HSP70 gene expression in bile duct carcinoma and normal bile duct. RNA interference was used to silence and inhibit HSP70 gene expression in HUC-T1 human bile duct cancer cells, and then CCK-8 assay and Transwell assay were used to detect the effects of downregulation of HSP70 gene expression on the proliferation, invasion and migration ability of HUC-T1 cells. Results The expression of HSP70 gene is abnormally high in the tissues and cells of cholangiocarcinoma. After inhibiting HSP70 gene expression in HUC-T1 cells, the proliferation, invasion and migration ability of HUC-T1 cells were significantly decreased. Conclusion HSP70 plays important roles in promoting tumorigenesis and development in bile duct cancer cells, and it might be used as a therapeutic target of bile duct cancer in further study.

Key words： heat shock protein 70 cholangiocarcinoma proliferation invasion migration

收稿日期: 2024-05-24

基金资助:湖南省财政厅2022年创新计划项目课题“DAMP分子HSP70通过TLR4途径激活MAPK调控胆管癌机制研究”（H1617）

通讯作者: 舒升光,E-mail：919434903@qq.com

引用本文:

张豹, 陈宏伟, 曾晓晨, 舒升光. HSP70基因对胆管癌细胞增殖、侵袭及迁移的影响[J]. 湖南师范大学学报(医学版), 2024, 21(4): 24-28. ZHANG Bao, CHEN Hongwei, ZENG Xiaochen, SHU Shengguang. The Effect of HSP70 Gene on the Proliferation, Invasion and Migration of Cholangiocarcinoma Cells. HuNan ShiFan DaXue XueBao(YiXueBan), 2024, 21(4): 24-28.

链接本文:

http://yxb.hunnu.edu.cn/CN/ 或 http://yxb.hunnu.edu.cn/CN/Y2024/V21/I4/24

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