Abstract：Objective To investigate the effect of UA on renal carcinoma 786-O cells and the potential mechanism. Methods Pubchem and Swiss target Prediction databases were used to search the structure and target of UA, and the promising targets of UA were picked out according to the probability of action marks, which ware greater than 0. The effects of UA on the viability, migration and invasion of 786-O cells were detected by CCK-8, flow cytometry, Transwell migration and invasion assay, respectively. The expression levels of P62, caspase3, lc3, Bcl-2 and Bax proteins were detected by Western blotting. Results Most of the predicted targets of UA were kinases and cyclins. UA inhibited the proliferation of 786-O cells in a concentration-dependent manner, as well as inhibited the expression of p62 and bcl2 in 786-O cells. And UA inhibited the migration and invasion ability. Moreover, the expression of caspase3, bax and lc3-II were down-regulated in 786-O cells. Conclusion UA can inhibit proliferation, induce apoptosis, inhibit migration and invasion in renal carcinoma cells. The mechanism may be related to inhibiting p62, bcl2 expression and promoting caspase3, bax and lc3-II expression in renal carcinoma cells.
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2024, Vol. 21 
