DFOG inhibits invasion and migration of SKOV3 ovarian cancer stem-like cells in vitro via down-regulating CD44 expression
LIN Jiazhi1, LIU Huiling1, DENG Yu'ao2, ZHANG Chen3, NING Yingxia1
1. Department of Gynecology and Obstetrics, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China; 2. Department of Gynecology and Obstetrics, Shenzhen People's Hospital, Shenzhen 518020, China; 3. Department of Clinical Pharmacy, the Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou 510120, China
Abstract：Objective To determine whether 7-Difluoromethoxyl-5, 4'-di-n-octyl genistein (DFOG) inhibits invasion and migration capabilities in SKOV3-derived ovarian cancer stem-like cells (OCSLCs) by downregulating the expression of CD44, a cancer stem cell surface marker. Methods CD44 gene was knocked down using the lentiviral delivery system. OCSLCs cells were treated with DFOG (0.1, 1.0 and 10 μM) or in combination with DFOG (10 μM) and shCD44. The expression levels of CD44 protein in OCSLCs infected with CD44 shRNA and treated with DFOG (0.1, 1.0 and 10.0 µM) were detected using western blot analysis. The cell invasion rates were determined using transwell invasion assay and cell migration rates were determined using Wound-Healing assay. Results CD44 knockdown significantly reduced the cell invasion rates and cell migration rates in OCSLCs cells. DFOG downregulated the expression of CD44 as well as attenuated cell invasion and cell migration capabilities in OCSLCs. Moreover, knockdown of CD44 gene collaborated with DFOG to inhibit the ability of cell invasion and cell migration in OCSLCs. Conclusion DFOG inhibits cell invasion and cell migration capabilities by downregulating CD44 expression in OCSLCs .
林佳芝, 刘会玲, 邓宇傲, 张晨, 宁映霞. DFOG下调CD44表达抑制SKOV3卵巢癌干样细胞体外侵袭和迁移[J]. 湖南师范大学学报(医学版), 2023, 20(2): 16-21.
LIN Jiazhi, LIU Huiling, DENG Yu'ao, ZHANG Chen, NING Yingxia. DFOG inhibits invasion and migration of SKOV3 ovarian cancer stem-like cells in vitro via down-regulating CD44 expression. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(2): 16-21.
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2023, Vol. 20 
