Differential Expression and Clinical Significance of CHAC1 Based on Bioinformatics Analysis in Pan-cancer

ZHEN Yao; OU Pengfei; XIA Jie; CHEN Gonghua; MAN Jing

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Journal of Hunan Normal University(Medical Science) ›› 2026, Vol. 23 ›› Issue (1) : 51-60.

Basic Medicine

Differential Expression and Clinical Significance of CHAC1 Based on Bioinformatics Analysis in Pan-cancer

ZHEN Yao^1,3, OU Pengfei², XIA Jie³, CHEN Gonghua³, MAN Jing³

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Abstract

Objective To explore the potential role of CHAC1 in pan-cancer biology by analyzing its correlation with clinical prognosis and immune characteristics using bioinformatics methods. Methods Data from multiple databases were used to investigate the differential expression of CHAC1 across various cancers, survival analysis, immune cell infiltration, tumor stemness score (DNAss), tumor mutational burden (TMB), and microsatellite instability (MSI) were also evaluated in relation to CHAC1 expression. Western blot analysis was employed to determine CHAC1 expression levels in gastric, lung, and colon cancer cell lines, while immunohistochemistry (IHC) was utilized to evaluate CHAC1 expression in human gastric carcinoma tissues and corresponding adjacent non-tumor tissues. Results CHAC1 was found to be highly expressed in multiple tumor types, with patients exhibiting poorer prognosis. The results of Western blot and IHC demonstrated that CHAC1 is highly expressed in gastric cancer. Immune cell infiltration, including CD8⁺ cells, CD4⁺ cells, neutrophils, dendritic cells, and macrophages, was significantly correlated with CHAC1 expression. Additionally, DNA methylation, DNAss, TMB, and MSI were found to be associated with CHAC1 expression levels. Conclusion CHAC1 may serve as a valuable prognostic biomarker and a potential therapeutic target for multiple cancers. Its role in immune filtration and immune checkpoint inhibitor therapy warrants further investigation.

Key words

CHAC1 / pan-cancer / bioinformatics / immune cell infiltration

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ZHEN Yao, OU Pengfei, XIA Jie, CHEN Gonghua, MAN Jing. Differential Expression and Clinical Significance of CHAC1 Based on Bioinformatics Analysis in Pan-cancer[J]. Journal of Hunan Normal University(Medical Science). 2026, 23(1): 51-60

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