Objective To explore whether oxycodone improves postoperative cognitive decline in elderly fracture models by affecting the AMPK/SIRT1 signaling pathway. Methods SD rats were randomly divided into sham operation group, model group, and 10, 20, and 30 μg·kg-¹·d-¹ oxycodone dose groups, with 10 rats in each group Open tibial fracture surgery was used to construct a model of cognitive dysfunction after fracture surgery in the elderly After 2 weeks of dosing, cognitive function was assessed using the Morris water maze test. The pathological injury of hippocampus and the number of neurons in hippocampus were observed by HE staining and Nissl staining, and the level of interleukin (IL) -1β, IL-18, tumor necrosis factor-α (TNF-α) in hippocampus was detected by ELISA. The level of p-AMPK/AMPK, SIRT1, p-NF-κB/NF-κB protein in hippocampus was detected by Western blot. Results Compared with the sham operation group, the model group showed a notable decrease in cognitive function (P<0.05), and the hippocampal tissue presented pathological damage, and the number of neurons in hippocampus decreased, and the IL-1β, IL-18 and TNF-α in hippocampus up-regulated whereas the p-AMPK/AMPK and SIRT1 down-regulated (P<0.05). After the treatment of 20, 30 μg·kg-¹·d-¹ oxycodone, the above phenomena were significantly improved, and the high dose was better. Conclusion Oxycodone may inhibit the expression of hippocampal pro-inflammatory factors by activating the AMPK/SIRT1 signaling pathway, thereby improving the postoperative cognitive decline in elderly fracture models
Key words
oxycodone /
AMPK/SIRT1 signaling pathway /
senile fracture /
postoperative cognitive decline
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