Objective To investigate the effect of regulatory T cells (Tregs) on the long-term behavior of neurological function in mice with hypoxic-ischemic encephalopathy (HIE), i. e. , learning and memory functions. Methods Six were randomly selected from 24 mice as the sham group. The remaining 18 models were modeled using the Rice-Vannucci method. Before modeling, 18 mice were randomly divided into experimental group (HIBD) group, treatment (Tregs) group, and depletion (CD25) group, with 6 mice in each group. The experimental group made normal molds; The treatment group was injected with Tregs through the ventricle within 72 hours after modeling. The depletion group was intraperitoneally injected with anti-CD25 antibody 1 day before modeling. Statistical analysis of mouse behavior was performed after modeling and intervention. Results After modeling, compared with the sham operation group, the cerebral blood flow on the side of the model was significantly reduced. In the water maze experiment (Morris Water Maze), the comparison of the waiting time in the target quadrant was significantly prolonged between the SHAM group and the HIBD group and the CD25 group, and between the Tregs group and the HIBD group and the CD25 group. Novel Object Recognition: Compared with the SHAM group, HIBD group, and CD25 group, the Tregs group had a significantly higher recognition index (RI) compared with the HIBD group and the CD25 group. Conclusion Treg cells can exert a neuroprotective effect in the treatment of ischemic-hypoxic brain injury.
Key words
ischemic-hypoxic encephalopathy /
regulatory T cells /
ventricular injection /
behavioral tests /
morris water maze /
novel object recognition
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