Esketamine regulation miR-146a expression to alleviate inflammation and lung injury in sepsis-induced acute lung injury

YANG Wenyu; YU Feng; LI Moyong; ZHANG Jingjing; CHEN Anji

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Journal of Hunan Normal University(Medical Science) ›› 2025, Vol. 22 ›› Issue (3) : 15-19.

Basic Medicine

Esketamine regulation miR-146a expression to alleviate inflammation and lung injury in sepsis-induced acute lung injury

YANG Wenyu^1,2, YU Feng³, LI Moyong^1,2, ZHANG Jingjing¹, CHEN Anji¹

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Abstract

Objective To investigate whether esketamine exerts a protective effect against sepsis-induced acute lung injury (ALI) by regulating miR-146a expression. Methods A sepsis-induced ALI mouse model was established using lipopolysaccharide (LPS, 10 mg/kg). The therapeutic efficacy of esketamine was assessed via two administration routes: intratracheal instillation and intraperitoneal injection. Pathological changes in lung tissue were evaluated by histopathological examination. The lung wet/dry weight ratio (W/D) was calculated to assess pulmonary edema. Levels of IL-6, TNF-α, and IL-1β in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA). The expression levels of miR-146a, IL-6, TNF-β, and IL-1β in lung tissues were quantified via qRT-PCR. Results Esketamine alleviated LPS-induced lung injury in septic mice and significantly suppressed the expression of IL-6, TNF-α, and IL-1β in BALF and lung tissues. Concurrently, esketamine upregulated miR-146a levels. Conclusion Esketamine mitigates sepsis-induced ALI by activating miR-146a expression and suppressing NF-κB pathway-mediated inflammatory responses. Intraperitoneal administration demonstrated superior efficacy compared to intratracheal instillation, highlighting its potential as a clinical therapeutic strategy.

Key words

esketamine / miR-146a / sepsis / acute lung injury / intraperitoneal injection

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YANG Wenyu, YU Feng, LI Moyong, ZHANG Jingjing, CHEN Anji. Esketamine regulation miR-146a expression to alleviate inflammation and lung injury in sepsis-induced acute lung injury[J]. Journal of Hunan Normal University(Medical Science). 2025, 22(3): 15-19

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