Objective To investigate the protein expressions of T-cell immunoglobulin mucin molecule 3(TIM-3), sialic acid binding Ig like lectin 9 (SIGLEC9) and Yes associated protein 1 (YAP1) in cancer tissues of patients with cervical cancer and the relationship with pathological characteristics and prognosis. Methods 82 patients with radical resection of cervical cancer were enrolled in Weifang People's Hospital from July 2020 to July 2022. The protein expressions of TIM-3, SIGLEC9 and YAP1 in cancer tissues and adjacent tissues were counted, and the relationship between protein expressions of TIM-3, SIGLEC9 and YAP1 in cancer tissues and clinicopathology was analyzed. According to the survival prognosis status of patients within 2 years of follow-up, they were divided into good prognosis group and poor prognosis group. The differences in the protein expression of TIM-3, SIGLEC9 and YAP1 in cancer tissues of patients with different survival status were compared. Kaplan-Meier survival analysis was used to analyze the relationship between TIM-3, SIGLEC9, YAP1 proteins in cancer tissues and prognosis. Results The positive expressions of TIM-3 in cancer tissues and adjacent tissues were 68.29% (56/82) and 6.10% (5/82), and the positive expressions of SIGLEC9 were 60.98% (50/82) and 2.44% (2/82), and the positive expressions of YAP1 were 76.83% (63/82) and 6.10% (5/82). The protein expression levels of TIM-3, SIGLEC9 and YAP1 in cancer tissues were significantly correlated with clinical staging, differentiation degree, depth of cervical invasion and lymph node metastasis. The positive expression rates of TIM-3, SIGLEC9 and YAP1 proteins in patients with high clinical stage, low differentiation degree, high depth of cervical invasion and lymph node metastasis were higher. The positive expression rates of TIM-3, SIGLEC9 and YAP1 proteins in poor prognosis group were significantly higher than those in good prognosis group. The progression-free-survival (PFS) in TIM-3 positive group was statistically different from TIM-3 negative group (log rank χ2=4.846, P=0.028). The PFS was statistically different between SIGLEC9 positive group and SIGLEC9 negative group (log rank χ2=4.112, P=0.043). The PFS of YAP1 in positive group and YAP1 negative group was statistically different (log rank χ2=10.011, P=0.002). Conclusion Patients with high clinical stage, low degree of differentiation, high depth of cervical invasion and lymph node metastasis are more likely to have positive protein expressions of TIM-3, SIGLEC9 and YAP1 in cancer tissues, and the positive expressions of the above proteins are related to poor prognosis.
Key words
cervical cancer /
T-cell immunoglobulin mucin molecule 3 /
sialic acid binding Ig like lectin 9 /
Yes associated protein 1 /
pathological characteristics /
prognostic analysis
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