Objective To establish a method for analyzing the composition of plasma fatty acid profiles in overweight and obese children based on online derivatization-gas chromatography (GC) -mass spectrometry (MS). Methods After extraction of 50 μL of children's plasma samples, 50 μL of derivatization reagent TMSH was added. The GC injection temperature was 320℃ and the residence time was 3s to achieve the instant derivatization of the derivatization reagent and fatty acid. Results A total of 11 fatty acid components were detected, and the relative standard deviations (RSD) of their relative contents were all within 20% (n=5). The plasma fatty acids of the children population were mainly composed of C16: 0, C18: 0, C18: 1n-9 and C18: 2n-6, accounting for more than 90% of the total fatty acids. Compared with normal children, the relative contents of C20: 2n-6 and C20: 3n-6 in the plasma of obese and overweight children were higher, and the relative contents of C14: 0 and C20: 3n-6 in the obese group were also higher. Compared with the overweight group, the levels of C16: 0 and SFAs in obese children were higher, while the levels of C18: 2n-6, n-6 PUFAs and PUFAs were lower, and the differences were statistically significant. Conclusion The online derivatition-GC-MS method is simple, rapid and accurate, which provides a new analytical method for the analysis of fatty acid profile in plasma. There are differences in the composition of plasma fatty acid profiles in children with different overweight and obesity status, which may be used as biomarkers for overweight and obesity in children.
Key words
online derivatization /
gas chromatography-tandem mass spectrometry /
fatty acids /
overweight and obesity
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References
[1] CAI X, SONG S, HU J, et al.Body Roundness Index Improves the Predictive Value of Cardiovascular Disease Risk in Hypertensive Patients with Obstructive Sleep Apnea: A Cohort Study[J]. Clin Exp Hypertens, 45(1): 2259132.
[2] DATTA B K, COUGHLIN S S, GUMMADI A, et al.Perceived Social Support and Cardiovascular Risk among Nonelderly Adults in the United States[J]. Am J Cardiol, 2023, 209: 146-153.
[3] ALMANDOZ J P, LINGVAY, ILDIKO.Encouraging but Slow Progress toward Incorporating Obesity Care into Diabetes Care[J]. Obesity, 2023, 31(12): 2893-2894.
[4] 张雁宇, 吕欣, 黄晓光, 等. 单不饱和脂肪酸作用机制及在糖尿病肾脏疾病中的研究进展[J]. 中国中西医结合肾病杂志, 2024, 25: 274-276.
[5] 阿尔兹海默症发生机制及脂质代谢在其中的作用[J]. 湖南师范大学医学学报 (医学版), 2023, 20(3): 1-9.
[6] GOLDSCHMIDT R B, WILLIAM. Gc Analysis of Seven Seed Oils Containing Conjugated Fatty Acids[J]. Separations, 2021, 8(4): 51.
[7] JOVANOVIC M S, SIGURD MITTELBACH, MARTIN. Gc Analysis of Primary Fatty Acid Amides in Animal Fat[J]. Eur J Lipid Sci Tech, 2021, 123(7): 1.
[8] JARUKAS L, KURAITE G, BARANAUSKAITE J, et al.Optimization and Validation of the Gc/Fid Method for the Quantification of Fatty Acids in Bee Products[J]. Applied Sciences, 2020, 11(1): 83.
[9] FILHO C B, FURLAN J M, MENEZES C R D, et al. Sample Preparation Methods for Fatty Acid Analysis in Different Raw Meat Products by Gc-Fid[J]. Food Analytical Methods, 2023, 16 749-16 758.
[10] HODSON L S, C. MURRAY FIELDING, BARBARA A. Fatty Acid Composition of Adipose Tissue and Blood in Humans and Its Use as a Biomarker of Dietary Intake[J]. Progress in Lipid Research, 2008, 47(5): 348-380.
[11] FIRL N, KIENBERGER H, HAUSER T, et al.Determination of the Fatty Acid Profile of Neutral Lipids, Free Fatty Acids and Phospholipids in Human Plasma[J]. Clin Chem Lab Med, 2013, 51(4): 799-810.
[12] FENG X, LIU N, YANG Y, et al.Isotope-Coded Chemical Derivatization Method for Highly Accurately and Sensitively Quantifying Short-Chain Fatty Acids[J]. J Agric Food Chem, 2022, 70(20): 6253-6263.
[13] XING L, ZHANG Q, LIU J, et al.Determination of Six Short‐Chain Fatty Acids in Rat Feces Using Headspace Solid‐Phase Dynamic Extraction Coupled with Gc‐Ms[J]. J Sep Sci, 2024, 47(12): e2400032.
[14] AKOTO L, VREULS R J, IRTH H, et al.Fatty Acid Profiling of Raw Human Plasma and Whole Blood Using Direct Thermal Desorption Combined with Gas Chromatography-Mass Spectrometry[J]. J Chromatogr A, 2008, 1186(1-2): 365-371.
[15] GOMES REIS M, DOS REIS M M, LEATH S, et al. Direct Analysis of Fatty Acid Profile from Milk by Thermochemolysis-Gas Chromatography-Mass Spectrometry[J]. J Chromatogr A, 2011, 1218(2): 316-323.
[16] RUDOLPH M C, YOUNG B E, LEMAS D J, et al.Early Infant Adipose Deposition Is Positively Associated with the N-6 to N-3 Fatty Acid Ratio in Human Milk Independent of Maternal Bmi[J]. Int J Obes (2005), 2017, 41(4): 510-517.
[17] FEKETE K, GYOREI E, LOHNER S, et al.Long-Chain Polyunsaturated Fatty Acid Status in Obesity: A Systematic Review and Meta-Analysis[J]. Obes Rev, 2015, 16(6): 488-497.
[18] GUERENDIAIN M, MONTES R, LOPEZ-BELMONTE G, et al.Changes in Plasma Fatty Acid Composition Are Associated with Improvements in Obesity and Related Metabolic Disorders: A Therapeutic Approach to Overweight Adolescents[J]. Clin Nutr, 2018, 37(1): 149-156.
[19] KAIKKONEN J E, JULA A, VIIKARI J S A, et al. Associations of Serum Fatty Acid Proportions with Obesity, Insulin Resistance, Blood Pressure, and Fatty Liver: The Cardiovascular Risk in Young Finns Study[J]. J Nutr, 2021, 151(4): 970-978.
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