Experimental study on the preparation method of acellular dermal matrix

LIU Jie; LIU Qiao; ZHOU Yongzhu; ZHU Yunping; YU Xing

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Journal of Hunan Normal University(Medical Science) ›› 2025, Vol. 22 ›› Issue (2) : 119-123.

Clinical Medicine

Experimental study on the preparation method of acellular dermal matrix

LIU Jie^1,2#, LIU Qiao^1#, ZHOU Yongzhu^1,2, ZHU Yunping², YU Xing¹

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Abstract

Objective To investigate an efficient and reliable method for the preparation of acellular dermal matrix. Methods Porcine skin was selected as the experimental material. Decellularization was performed using a decellularization solution composed of phospholipase A2 and sodium deoxycholate, followed by washing with a 3.4M NaCl high-salt solution and DNase solution. The prepared acellular dermal matrix was characterised using DAPI staining, hematoxylin and eosin (H&E) staining, and immunohistochemical analysis. Additionally, the quantitative assessment of collagen and other components was conducted. Results The prepared matrix demonstrated effective decellularization, exhibiting a high retention of major components such as collagen. Conclusion The decellularization method that employs a combination of phospholipase A2 and sodium deoxycholate is characterised by its efficiency, simplicity, and effectiveness. It fulfils the requirements for decellularization and is a time-efficient process. The resulting dermal matrix exhibits structural integrity and biochemical components, rendering it an ideal biomedical material.

Key words

decellularization / decellularized dermal matrix / biomedical material

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LIU Jie, LIU Qiao, ZHOU Yongzhu, ZHU Yunping, YU Xing. Experimental study on the preparation method of acellular dermal matrix[J]. Journal of Hunan Normal University(Medical Science). 2025, 22(2): 119-123

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