Objective To investigate the target and potential mechanism of the action of Artemisia annua in the treatment of acute myocardial infarction (AMI) based on traditional Chinese medicine network pharmacology and in vitro experiments. Methods The chemical active ingredients and target genes of Artemisia annua were retrieved from TCMSP and Uniprot databases. We then searched for AMI-related genes using GeneCards, OMIM, TTD, PharmGKB, and DrugBank databases, screened out the common targets of Artemisia annua for the treatment of AMI, constructed a drug-disease-target network using Cytoscape 3.8.0 software, and constructed a protein-protein interaction(PPI) network using the String database. Core genes were screened out and analysed for GO functional enrichment and KEGG pathway enrichment. The effects of artemisinin (ART) on CoCl2-induced proliferation and apoptosis in H9C2 cells were assessed in in vitro studies. Results 20 active ingredients were obtained from Artemisia annua, including isorhamnetin, quercetin, artemisinin, and dihydroartemisinin.193 targets were obtained by removing duplicates.3278 targets were related to AMI, and 155 drug-disease co-targets were screened out, including TP53, AKT1, MAPK1, RELA, ESR1, and MAPK14. And the GO function enrichment analysis showed that the biological processes and function of Artemisia annua in the treatment of AMI were mainly focused on oxidative stress, inflammation, and xenograft rejection et al. KEGG pathway enrichment analysis showed that the treatment of AMI by Artemisia annua were mainly related to AGE-RAGE signaling pathway, TNF pathway, IL-17 pathway, PI3K-Akt pathway and apoptosis. ART promoted H9C2 cell proliferation and inhibited apoptosis and in vitro. Conclusion The study elucidated the potential targets of Artemisia annua L. and its active compound, artemisinin, in the treatment of AMI. It offers novel insights and methods for the application of traditional Chinese medicine in managing AMI.
Key words
artemisia annua /
myocardial infarction /
network pharmacology /
experimental validation
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