Objective The aim of this study was to evaluate the food safety and antioxidant function of S. miltiorrhiza leaf extracts, so as to provide a basis for its research and development and popularization in the field of medicinal and food applications. Methods The safety of S. miltiorr0hiza leaf extract was evaluated using acute toxicity, Ames, mammalian erythrocyte micronucleus test, in vitro mammalian cell chromosome aberration test and 90-day oral toxicity test in rats, and at the end of the 90-day oral toxicity test, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the serum and liver tissues of rats and the malondialdehyde (MDA) content. Results The oral LD50 of S. miltiorrhiza leaf extract in female and male rats was more than 10 g/kg, which was non-toxic, and the results of three genotoxicity tests were negative. The results of the 90-day feeding experiment in rats (general observation; body weight, food intake, and food utilization; hematology and blood biochemistry; urine, gross anatomy, and histopathological effects in rats) did not show any significant toxicity. The levels of SOD and GSH-Px in serum and liver tissues of female and male rats in the low-dose, medium-dose and high-dose groups were significantly higher than those in the control group. The levels of MDA in the group were significantly lower than those in the control group. Conclusion S. miltiorrhiza leaf extract was non-toxic under the experimental conditions, and did not show acute, sub-chronic toxicity and genotoxicity; the results of oxidative stress indexes in serum and liver tissues suggested that S. miltiorrhiza leaf extract had certain antioxidant functions.
Key words
Salvia miltiorrhiza leaf extract /
antioxidant /
acute toxicity /
genotoxicity /
subchronic toxicity
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