The Effect of HSP70 Gene on the Proliferation, Invasion and Migration of Cholangiocarcinoma Cells

ZHANG Bao; CHEN Hongwei; ZENG Xiaochen; SHU Shengguang

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Journal of Hunan Normal University(Medical Science) ›› 2024, Vol. 21 ›› Issue (4) : 24-28.

Basic Medicine

The Effect of HSP70 Gene on the Proliferation, Invasion and Migration of Cholangiocarcinoma Cells

ZHANG Bao, CHEN Hongwei, ZENG Xiaochen, SHU Shengguang

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Abstract

Objective To study the effect of HSP70 gene on proliferation, invasion and migration of bile duct cancer cells. Methods qPCR and Western blot were used to detect the difference of HSP70 gene expression in bile duct carcinoma and normal bile duct. RNA interference was used to silence and inhibit HSP70 gene expression in HUC-T1 human bile duct cancer cells, and then CCK-8 assay and Transwell assay were used to detect the effects of downregulation of HSP70 gene expression on the proliferation, invasion and migration ability of HUC-T1 cells. Results The expression of HSP70 gene is abnormally high in the tissues and cells of cholangiocarcinoma. After inhibiting HSP70 gene expression in HUC-T1 cells, the proliferation, invasion and migration ability of HUC-T1 cells were significantly decreased. Conclusion HSP70 plays important roles in promoting tumorigenesis and development in bile duct cancer cells, and it might be used as a therapeutic target of bile duct cancer in further study.

Key words

heat shock protein 70 / cholangiocarcinoma / proliferation / invasion / migration

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ZHANG Bao, CHEN Hongwei, ZENG Xiaochen, SHU Shengguang. The Effect of HSP70 Gene on the Proliferation, Invasion and Migration of Cholangiocarcinoma Cells[J]. Journal of Hunan Normal University(Medical Science). 2024, 21(4): 24-28

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