Decitabine inhibits migration and invasion of hepatocellular carcinoma cells by upregulating SOCS1 expression

DENG Tingyun; LOU Lei; WANG Lianghou; ZHANG Jiansong; LI Xiang

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Journal of Hunan Normal University(Medical Science) ›› 2024, Vol. 21 ›› Issue (4) : 1-6.

Basic Medicine

Decitabine inhibits migration and invasion of hepatocellular carcinoma cells by upregulating SOCS1 expression

DENG Tingyun, LOU Lei, WANG Lianghou, ZHANG Jiansong, LI Xiang

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Abstract

Objective This study was designed to investigate the effect of Decitabine (DAC) on hepatocellular carcinoma cell migration and invasion and to explore its molecular mechanisms. Methods MTT assay was used to detect the effect of different concentrations of DAC on the viability of hepatocellular carcinoma MHCC97H cells. The influence of different concentrations of DAC on the migratory and invasive abilities of hepatocellular carcinoma MHCC97H cells were determined by cell wound healing and transwell invasion assays. The impact of DAC on the protein expression of suppressor of cytokine signaling 1(SOCS1) in hepatocellular carcinoma MHCC97H and SK-Hep-1 cells was assessed by Western blot (WB). The siRNA transfection assay was performed to knock down the expression of SOCS1 in hepatocellular carcinoma MHCC97H cells, and changes in the migratory and invasive ability of cells were observed. Results DAC inhibited the viability of hepatocellular carcinoma MHCC97H cells in a concentration-dependent manner (IC₅₀=5.0 μM) and had the inhibitory effect on cell migration and invasion. Knocking down the expression of SOCS1 enhanced the migration and invasion of hepatocellular carcinoma MHCC97H cells. DAC upregulated the expression of SOCS1 in hepatocellular carcinoma cells (MHCC97H and SK-Hep-1) in a concentration-dependent manner, and DAC reversed the promotion of migration and invasion of hepatocellular carcinoma MHCC97H cells by low expression of SOCS1. Conclusion DAC inhibits migration and invasion of hepatocellular carcinoma cells by upregulating SOCS1 expression.

Key words

decitabine / suppressor of cytokine signaling 1(SOCS1) / hepatocellular carcinoma cells / migration / invasion

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DENG Tingyun, LOU Lei, WANG Lianghou, ZHANG Jiansong, LI Xiang. Decitabine inhibits migration and invasion of hepatocellular carcinoma cells by upregulating SOCS1 expression[J]. Journal of Hunan Normal University(Medical Science). 2024, 21(4): 1-6

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