Objective This study was designed to examine the effect of metformin on triggering receptors expressed on myeloid cells-1（TREM-1）in a lipopolysaccharide（LPS）induced acute lung injury（ALI）mouse model and explore whether metformin utilizes TREM-1/NF- κB pathway to reduce ALI in mice. Methods First of all,an ALI mouse model was established by intraperitoneal injection of 10 mg/kg LPS,and intervention with metformin or TREM-1 receptor antagonist was used to examine the effect of metformin on TREM-1 expression in ALI mice. Total protein level,total cell and neutrophil count,lactate dehydrogenase（LDH）and myeloperoxidase（MPO）were determined in the lavage fluid（BALF）,and the wet/dry specific gravity of lung tissues were assessed. NF-κB and TREM-1 mRNA expression was assessed through Real time-PCR,while NF-κB,IL-18,IL-1β,TNF-α and TREM-1 protein expression levels were measured by Western blots. Results Our results showed that metformin reduced the expression of TREM-1 in the lung tissue of ALI mice. After antagonizing TREM-1,metformin could alleviate pathological damage,protein exudation,and neutrophil infiltration in lung tissue,and expression of NF-κB,IL-18,IL-1β,TNF-α was inhibited. Conclusion Metformin can reduce the expression of inflammatory factors,vascular permeability,and infiltration of inflammatory cells,thereby alleviating LPS-induced ALI,and its mechanism may be related to TREM-1/NF-κ B pathway.
Key words
acute lung injury /
metformin /
triggering receptors expressed on myeloid cells-1（TREM-1） /
nuclear factor-κB
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