Effect of miR-567 and CacyBP on A549 cells of non-small cell lung cancer

LI Haiyang; ZHAO Zhenshan; LI Jing; RONG Yao; ZHENG Aiming; HAO Menghui; TIAN Faming

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Journal of Hunan Normal University(Medical Science) ›› 2024, Vol. 21 ›› Issue (1) : 15-20.

Basic Medicine

Effect of miR-567 and CacyBP on A549 cells of non-small cell lung cancer

LI Haiyang¹, ZHAO Zhenshan¹, LI Jing¹, RONG Yao¹, ZHENG Aiming², HAO Menghui¹, TIAN Faming³

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Abstract

Objective To investigate the effects of miR-567 and calcyin-binding protein (CacyBP) on proliferation, invasion and apoptosis of non-small cell lung cancer (NSCLC) A549 cells. Methods NSCLC A549 with high expression of miR-567 and CacyBP was selected as the study object. The experiment was divided into 4 groups, blank control group (NC group), transfected with pcDNA6.2 miR-567(si-miR-567 group). Transfected pcDNA6.2 CacyBP siRNA (si-CacyBP group) and pcDNA6.2 miR-567+CacyBP siRNA group (si-miR-567+CacyBP group); mRNA expressions of miR-567 and CacyBP were detected by qRT-PCR. Cell proliferation was detected by CCK-8 and MTT assay. Transwell assay was used to test cell migration and invasion. The apoptosis rate of NSCLC A549 cells was determined by flow cytometry. The changes of apoptotic proteins in each group were detected by Western bolt. Results Compared with NC group, the expression of miR-567 in si-miR-567 groupand si-miR-567+CacyBP group was significantly decreased. Compared with NC group, the CacyBP mRNA expression in si-CacyBP group and si-miR-567+CacyBP group was significantly decreased. The results of MTT assay showed that the cell proliferation inhibition rate of three groups was significantly higher than that of NC group, and the cell proliferation inhibition rate of si-miR-567+CacyBP group was significantly higher than that of si-miR-567 and si-CacyBP groups. The results of CCK-8 experiment showed that the cell proliferation count of three groups was significantly lower than that of NC group, and the cell proliferation count of si-miR-567+CacyBP group was significantly lower than that of si-miR-567 and si-CacyBP groups. Transwell experiment showed that the number of cell migration and invasion in NC group was significantly higher than that in si-miR-567 group, si-CacyBP group and si-miR-567+CacyBP group. The number of cell migration and invasion in si-miR-567 group and si-CacyBP group was significantly higher than that in si-miR-567+CacyBP group. Flow cytometry showed that compared with NC group, the apoptosis rate of A549 cells in si-miR-567 group, si-CacyBP group and si-miR-567+CacyBP group was significantly increased. The apoptosis rate of A549 cells in si-miR-567+CacyBP group was significantly higher than that in si-miR-567 and si-CacyBP groups. Conclusion Combined intervention of miR-567 and CacyBP can effectively reduce the proliferation, migration and invasion ability of NSCLC A549 cells, and increase the apoptosis rate of cells.

Key words

calcyclin binding protein / miR-567 / non-small cell lung cancer / cell proliferation / cell apoptosis / molecular mechanism

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LI Haiyang, ZHAO Zhenshan, LI Jing, RONG Yao, ZHENG Aiming, HAO Menghui, TIAN Faming. Effect of miR-567 and CacyBP on A549 cells of non-small cell lung cancer[J]. Journal of Hunan Normal University(Medical Science). 2024, 21(1): 15-20

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