Study on the correlation between the reduction of islet β-cell function and the ratio of GLP-1/GLU in severely scalded MODS mice

LUO Yanji, HUANG Zhihong, XIAO Chuangqing, YE Chunmei, ZHONG Jie, CAO Wenjing

Journal of Hunan Normal University(Medical Science) ›› 2023, Vol. 20 ›› Issue (1) : 24-30.

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Journal of Hunan Normal University(Medical Science) ›› 2023, Vol. 20 ›› Issue (1) : 24-30.
Basic Medicine

Study on the correlation between the reduction of islet β-cell function and the ratio of GLP-1/GLU in severely scalded MODS mice

  • LUO Yanji1, HUANG Zhihong2, XIAO Chuangqing2, YE Chunmei1, ZHONG Jie2, CAO Wenjing2
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Abstract

Objective To study the correlation between the reduction of islet β-cell function and the ratio of glucagon-like peptide 1/glucose (GLP-1/GLU) and explore its mechanism in severely scalded MODS mice so as to provide experimental basis for the prevention and treatment of the reduction of islet β-cell function in severely scalded MODS from a new perspective of improving the function of GLP-1 secretion in intestine. Methods 90 Kunming mice of Specific Pathogen Free (SPF) grade were randomly divided into three groups: 10 mice as the control group, 30 mice as simple scald group and 50 mice as MODS group. The mice in MODS group were made into MODS mice with 30% total body surface area Ⅲ degree scald and intraperitoneal injection of LPS. The mice in simple scald group were only inflicted with 30% total body surface area Ⅲ degree scald, and mice in the control group were sham scalded. Blood samples of mice in the control group were collected. At 1 day and 3 days after injury, 10 mice were randomly captured respectively from simple scald group and MODS group, and blood samples were collected. At 5 days after injury, blood samples of all surviving mice in scald group and MODS group were collected. The levels of GLU, plasma insulin, GLP-1 and diamine oxidase (DAO) of mice were determined. Homeostasis model assessment of β-cell function (HOMA-β) was applied, and then the index of islet β-cell function (HOMA-β) and the ratio of plasma GLP-1/GLU were calculated and observed. The correlation coefficient between HOMA-β and plasma GLP-1/GLU was calculated. The ileum tissues of mice were collected to observe the expression of GLP-1 by immunohistochemistry, and then the levels of intestinal GLP-1 secretion relative to blood glucose (intestinal GLP-1/GLU) were calculated and observed. The correlation coefficient between plasma GLP-1/GLU and intestinal GLP-1/GLU, and the correlation coefficient between intestinal GLP-1/GLU and plasma DAO were calculated. Data were statistically analyzed with one-way analysis of variance and Bonferroni test, Kruskal-Wallis and Bonferroni test, and Spearman rank correlation analysis. Results HOMA-β, plasma GLP-1/GLU and intestinal GLP-1/GLU of severely scalded MODS mice at 1, 3 and 5 days after injury were significantly lower than those of mice in the control group and simple scald group at the same time point (P<0.05). HOMA-β was positively correlated with plasma GLP-1/GLU of mice (r=0.685, P<0.001). Plasma GLP-1/GLU was positively correlated with intestinal GLP-1/GLU (r=0.851, P<0.001). Intestinal GLP-1/GLU was negatively correlated with DAO (r=-0.732, P<0.001). Conclusion The islet β-cell function and the ratio of GLP-1/GLU were decreased in severely scalded MODS mice. Insufficiency of plasma GLP-1 relative to blood glucose was correlated with the reduction of islet β-cell function. Intestinal damage led to relatively insufficient GLP-1 secretion. Protecting intestinal cells to increase the ratio of plasma GLP-1/GLU in severely scalded MODS mice can be used as an important measure to improve the reduction of islet β-cell function.

Key words

severe scalds / MODS / reduction of islet β-cell function / GLP-1/GLU / inflammatory cytokines

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LUO Yanji, HUANG Zhihong, XIAO Chuangqing, YE Chunmei, ZHONG Jie, CAO Wenjing. Study on the correlation between the reduction of islet β-cell function and the ratio of GLP-1/GLU in severely scalded MODS mice[J]. Journal of Hunan Normal University(Medical Science). 2023, 20(1): 24-30

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