The Expression And Function of PTPRO Gene Promoter Methylation in Colorectal Cancer

Journal of Hunan Normal University(Medical Science) ›› 2015, Vol. 12 ›› Issue (2) : 16-19.

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Journal of Hunan Normal University(Medical Science) ›› 2015, Vol. 12 ›› Issue (2) : 16-19.

The Expression And Function of PTPRO Gene Promoter Methylation in Colorectal Cancer

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Abstract

Objective Research the PTPRO Gene promoter methylation and the expression of PTPRO gene mRNA in colorectal cancer tissue and normal tissue adjacent to carcinoma,and to discuss their relationship in colorectal cancer. Methods Methylation specific PCR (MSP) and RT-PCR were use to detected PTPRO gene promoter methylation status and mRNA’s expression in 30 cases of colorectal cancer tissue and in normal tissue adjacent to carcinoma. Results The PTPRO gene promoter methylation rate of colorectal cancer tissues is higher than normal tissue adjacent to carcinoma,the PTPRO gene mRNA’s expression of the methylation samples is obviously inhibitive. Conclusion The PTPRO gene promoter methylation plays an important role in colorectal cancer, and it may be related to the development and prognosis of colorectal cancer

Key words

colorectal cancer / PTPRO / promoter / DNA methylation

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The Expression And Function of PTPRO Gene Promoter Methylation in Colorectal Cancer[J]. Journal of Hunan Normal University(Medical Science). 2015, 12(2): 16-19

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