黄花倒水莲口山酮类成分的分离鉴定及抗肿瘤活性筛选

马珊珊; 杨茗晰; 文丹丹; 季艳; 何思颖; 邹辉; 周子虬; 龙家印

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湖南师范大学学报医学版 ›› 2026, Vol. 23 ›› Issue (1) : 197-202.

药学

黄花倒水莲口山酮类成分的分离鉴定及抗肿瘤活性筛选

马珊珊¹, 杨茗晰¹, 文丹丹¹, 季艳¹, 何思颖¹, 邹辉¹, 周子虬^1,2,*, 龙家印^1,*

作者信息 +

Isolation, Identification and Antitumor Activity Screening of Xanthones from Polygala fallax Hemsl

MA Shanshan¹, YANG Mingxi¹, WEN Dandan¹, JI Yan¹, HE Siying¹, ZOU Hui¹, ZHOU Ziqiu^1,2, LONG Jiayin¹

Author information +

文章历史 +

摘要

目的对黄花倒水莲干燥根50%乙醇提取物的成分进行了分离鉴定,旨在寻求天然的、具有抗肿瘤作用的活性成分。方法将黄花倒水莲根粉碎,采用50 %乙醇充分浸泡48 h,加热回流提取,减压浓缩得到黄花倒水莲流浸膏,流浸膏再经过大孔吸附树脂柱层析、硅胶柱层析以及Semi-preparative HPLC等技术得到分离与纯化,运用UV、HR-ESI-MS、NMR等光谱学技术对其结构进行确证。采用MTT法评价系列口山酮类化合物对膀胱癌细胞UMUC3、人肝癌细胞HepG2、人脑胶质瘤细胞T98G、人胃癌细胞SGC-7901以及人乳腺癌细胞MDA-MB-231的细胞毒作用。结果本实验从黄花倒水莲根中共分离鉴定单体化合物8个,均为口山酮类。化合物5是首次从自然界中发现的新天然产物,化合物2首次从黄花倒水莲中分离得到,化合物4对人胃癌细胞SGC-7901和人乳腺癌细胞MDA-MB-231表现出一定的细胞毒作用,其IC₅₀值分别为41.931 μM以及56.544 μM。结论本实验从黄花倒水莲中共分离鉴定了单体化合物8个,包括1个首次发现的新天然产物,筛选得到具有较好体外抗肿瘤活性的化合物1个。

Abstract

Objective To separate and determine the chemical constituents of the 50% ethanol extract from the dried roots of Polygala fallax Hemsl, intended to find natural active ingredients with good anticancer activities. Methods The air-dried-crushed roots of Polygala fallax Hemsl were soaked in 50% aqueous ethanol for 48h, heating reflux extraction and vacuum concentration to get the concentrated liquid. The concentrated liquid was subjected on a macroporous adsorption resin D101 column and a silica gel column, then edulcorated by Semi-preparative HPLC. The structures of all compounds were identified by modern technologies such as UV, HR-ESI-MS, IR and NMR. MTT assay was used to evaluate the cytotoxicity of xantones on bladder cancer cell UMUC3, human hepatoma cell HepG2, human glioma cell T98G, human gastric cancer cell SGC-7901 and human breast cancer cell MDA-MB-231. Results In this paper, eight xanthones were isolated from the traditional Chinese medicine P. fallax Hemsl. Compound 5 was first isolated from nature and compound 2 was first isolated from P. fallax Hemsl. Compound 4 exhibited moderate cytotoxicity with IC₅₀ of 41.931 μM and 56.544 μM against human gastric carcinoma SGC-7901 cells and human breast cancer MDA-MB-231 cells respectively. Conclusion In this study, eight monomeric compounds were isolated and elucidated from the roots of P. fallax Hemsl, including one new discovered naturalproduct. One compound with good antitumor activity in vitro was selected.

导出引用

马珊珊, 杨茗晰, 文丹丹, 季艳, 何思颖, 邹辉, 周子虬, 龙家印. 黄花倒水莲口山酮类成分的分离鉴定及抗肿瘤活性筛选[J]. 湖南师范大学学报医学版. 2026, 23(1): 197-202

MA Shanshan, YANG Mingxi, WEN Dandan, JI Yan, HE Siying, ZOU Hui, ZHOU Ziqiu, LONG Jiayin. Isolation, Identification and Antitumor Activity Screening of Xanthones from Polygala fallax Hemsl[J]. Journal of Hunan Normal University(Medical Science). 2026, 23(1): 197-202

中图分类号： R932

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基金

湖南省教育厅优秀青年项目“基于‘化学成分谱-网络分析-分子机制评价’模式研究黄花倒水莲降血脂的药效物质基础”（24B0109）; 湖南省2023年大学生创新创业训练项目“中药黄花倒水莲抗凝血药效成分研究”（2023043）; 湖南师范大学2025年大学生创新创业训练项目“黄花倒水莲化学成分及抗凝血活性筛选”（2025459）