目的：探讨宫颈鳞状细胞癌（SCC）和高级别鳞状上皮内病变（HSIL）患者血液中微小RNAs（microRNA,miRNAs）的表达差异。方法：于2020年1月―2022年1月,纳入35名HSIL和35名宫颈SCC患者和30名健康对照进行研究。收集患者的血液标本,采用qRT-PCR分析血清中miR-409、miR-32、miR-454、miR-17、miR-520和miR-373的相对miRNA表达水平。采用接受者操作特征曲线（ROC）分析差异表达的miRNAs的诊断潜力。结果：与健康对照组比较,HSIL和SCC组织中miR-409表达显著下调,miR-32、miR-454、miR-17表达均显著上调。与HSIL组比较,SCC组的miR-409表达显著下调,miR-32、miR-454、miR-17表达均显著上调。ROC分析显示,miR-409（AUC=0.972）、miR-32（AUC=0.942）、miR-454（AUC=0.905）、miR-17（AUC=0.969）在区分对照组和SCC组方面具有高特异度和高敏感度。miR-409（AUC=0.921）、miR-17（AUC=0.851）在区分HSIL组和SCC组方面具有较高的特异度和敏感度。miR-32、miR-454在区分HSIL组和SCC组方面价值较低。结论：宫颈癌及癌前病变中的miRNAs（miR-409、miR-32、miR-454、miR-17）具有明显差异表达,且在区分正常和SCC方面显示出良好的敏感性和特异性。其中,miR-409和miR-17还可作为区分HSIL和SCC的潜在标志物。
Abstract
Objective To investigate the expression of miRNAs in the blood of patients with squamous cell carcinoma (SCC) and high-grade squamous intraepithelial lesion (HSIL) of the cervix and to determine the impact of these miRNAs in the clinical course of cervical cancer development. Methods Between January 2020 and January 2022, a total of 35 patients with HSIL and 35 patients with cervical SCC and 30 healthy controls were enrolled in the study. Blood specimens from patients were collected and the relative miRNA expression levels of miR-409, miR-32, miR-454, miR-17, miR-520 and miR-373 in serum were analyzed by qRT-PCR. Receiver operating characteristic curve (ROC) was used to analyze the diagnostic potential of differentially expressed miRNAs. Results Compared with healthy controls, miR-409 was found to be significantly down-regulated, and miR-32, miR-454, and miR-17 were significantly up-regulated in HSIL and cervical SCC tissues. In addition, miR-409 was significantly downregulated, and miR-32, miR-454, and miR-17 were significantly upregulated in the cervical SCC group compared with the HSIL group. roC analysis showed that miR-409(AUC=0.972), miR-32(AUC=0.942), and miR-454(AUC=0.905), and miR-17(AUC=0.969) had high specificity and sensitivity in distinguishing the control and SCC groups. miR-409(AUC=0.921) and miR-17(AUC=0.851) had high specificity and sensitivity in distinguishing the HSIL and SCC groups. In addition, miR-32 and miR-454 were less sensitive in distinguishing HSIL group from SCC group. Conclusion In this study, four miRNAs (miR-409, miR-32, miR-454, miR-17) differentially expressed in the sera of normal controls, HSIL and SCC patients are identified, and these miRNAs show good sensitivity and specificity in distinguishing normal from SCC. Among them, miR-409 and miR-17 could also be used as potential markers to distinguish HSIL from SCC.
关键词
microRNA /
宫颈癌 /
高级别鳞状上皮内病变
Key words
microRNA /
cervical cancer /
high-grade squamous intraepithelial lesion
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基金
陕西省卫生健康委员会科研项目（20210186）
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