白藜芦醇抑制TREM-1表达并减轻脂多糖诱导的小鼠急性肺损伤

周薇; 肖明雷; 韦运富; 刘思涵; 张哲; 郭加; 邓骏杰; 朱爱萍; 孙国瑛

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湖南师范大学学报医学版 ›› 2023, Vol. 20 ›› Issue (2) : 8-15.

基础医学

白藜芦醇抑制TREM-1表达并减轻脂多糖诱导的小鼠急性肺损伤

周薇, 肖明雷, 韦运富, 刘思涵, 张哲, 郭加, 邓骏杰, 朱爱萍, 孙国瑛

作者信息 +

Resveratrol inhibits TREM-1 expression and alleviates LPS-induced acute lung injury in mice

ZHOU Wei, XIAO Minglei, Wei Yunfu, LIU Sihan, ZHANG Zhe, GUO Jia, DENG Junjie, ZHU Aiping, SUN Guoying

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摘要

目的：观察白藜芦醇对急性肺损伤（acute lung injury,ALI）时髓样细胞触发受体1（triggering receptor expressed on myeloid cells-1,TREM-1）表达的影响,以及白藜芦醇对ALI小鼠肺损伤的影响。方法：采用致死剂量脂多糖（LPS）（50 mg/kg）作用于小鼠,观察白藜芦醇对LPS诱导的ALI小鼠存活率的影响;检测白藜芦醇对LPS（10 mg/kg）诱导的ALI小鼠呼吸功能的影响;观察白藜芦醇对ALI小鼠病理损伤、支气管肺泡灌洗液（BALF）中总蛋白水平、总细胞数、中性粒细胞数、LDH活性、肺组织TREM-1、IL-1β、IL-18的表达及MPO活性的影响。结果：白藜芦醇可改善LPS诱导的ALI小鼠的存活率和呼吸功能,减轻肺组织损伤、血管的通透性、炎症细胞的浸润,并减少炎性因子的表达。本研究首次观察到白藜芦醇可降低LPS应激的小鼠巨噬细胞TREM-1 mRNA的表达,其信号通路可能与MAPK及NF-κB有关。结论：白藜芦醇通过MAPK及NF-κB通路下调LPS诱导的ALI小鼠TREM-1的表达,并可减轻ALI。

Abstract

Objective To observe the effect of resveratrol on the expression of triggering receptor expressed on myeloid cells-1(TREM-1) during acute lung injury (ALI) and the effect of resveratrol on ALI mice. Methods We observe the effect of resveratrol on the survival rate of lipopolysaccharide (LPS) (Mice were treated with a lethal dose of LPS 50 mg/kg) -induced ALI mice; To detect the effect of resveratrol on the respiratory function of ALI mice induced by LPS (10 mg/kg); To observe the effect of resveratrol on the pathological damage, the level of total protein, total cell count, neutrophil count, LDH activity in bronchoalveolar lavage fluid (BALF), and the effect of resveratrol on TREM-1, IL-1β, IL-18 expression and MPO activity in lung tissue of ALI mice. Results We observed that resveratrol can improve the survival rate and respiratory function of LPS induced ALI mice, reduce pathological damage, vascular permeability, inflammatory infiltration, and decrease the expression of inflammatory factors of lung tissue. This study is the first to observe that resveratrol can reduce the expression of TREM-1 mRNA in macrophages of stressed by LPS, and its mechanism may be related to MAPK and NF-κB signal pathway. Conclusion Resveratrol downregulates the expression of TREM-1 in LPS-induced ALI mice through MAPK and NF-κB pathway, and can alleviate ALI.

导出引用

周薇, 肖明雷, 韦运富, 刘思涵, 张哲, 郭加, 邓骏杰, 朱爱萍, 孙国瑛. 白藜芦醇抑制TREM-1表达并减轻脂多糖诱导的小鼠急性肺损伤[J]. 湖南师范大学学报医学版. 2023, 20(2): 8-15

ZHOU Wei, XIAO Minglei, Wei Yunfu, LIU Sihan, ZHANG Zhe, GUO Jia, DENG Junjie, ZHU Aiping, SUN Guoying. Resveratrol inhibits TREM-1 expression and alleviates LPS-induced acute lung injury in mice[J]. Journal of Hunan Normal University(Medical Science). 2023, 20(2): 8-15

中图分类号： R363

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