DFOG下调CD44表达抑制SKOV3卵巢癌干样细胞体外侵袭和迁移

林佳芝; 刘会玲; 邓宇傲; 张晨; 宁映霞

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湖南师范大学学报医学版 ›› 2023, Vol. 20 ›› Issue (2) : 16-21.

基础医学

DFOG下调CD44表达抑制SKOV3卵巢癌干样细胞体外侵袭和迁移

林佳芝¹, 刘会玲¹, 邓宇傲², 张晨³, 宁映霞¹

作者信息 +

DFOG inhibits invasion and migration of SKOV3 ovarian cancer stem-like cells in vitro via down-regulating CD44 expression

LIN Jiazhi¹, LIU Huiling¹, DENG Yu'ao², ZHANG Chen³, NING Yingxia¹

Author information +

文章历史 +

摘要

目的：研究金雀异黄素类似物7-二氟甲氧基-5,4'-二正辛基金雀异黄素（7-difluoromethoxyl-5, 4'-di-n-octyl genistein,DFOG）能否通过下调肿瘤干细胞表面标志蛋白CD44表达抑制SKOV3源性卵巢癌干样细胞（OCSLCs）体外侵袭和迁移能力。方法：慢病毒递送系统敲低CD44基因表达（Lent-shCD44）、DFOG（0.1、1.0和10.0 µM）或DFOG（10 μM）联合Lent-shCD44处理OCSLCs细胞。免疫蛋白印迹法分析OCSLCs细胞 CD44蛋白的表达水平。Transwell小室侵袭试验检测OCSLCs细胞体外侵袭能力以及伤口愈合法检测OCSLCs体外迁移能力。结果：Lent-shCD44有效降低SKOV3源性OCSLCs细胞侵袭率和迁移能力。DFOG以剂量依赖方式下调OCSLCs细胞肿瘤干细胞标志物蛋白CD44表达和降低侵袭能力和迁移能力。此外,Lent-shCD44协作DFOG抑制OCSLCs细胞侵袭能力和迁移能力。结论：DFOG通过下调CD44蛋白表达抑制细胞OCSLCs细胞侵袭能力和迁移能力。

Abstract

Objective To determine whether 7-Difluoromethoxyl-5, 4'-di-n-octyl genistein (DFOG) inhibits invasion and migration capabilities in SKOV3-derived ovarian cancer stem-like cells (OCSLCs) by downregulating the expression of CD44, a cancer stem cell surface marker. Methods CD44 gene was knocked down using the lentiviral delivery system. OCSLCs cells were treated with DFOG (0.1, 1.0 and 10 μM) or in combination with DFOG (10 μM) and shCD44. The expression levels of CD44 protein in OCSLCs infected with CD44 shRNA and treated with DFOG (0.1, 1.0 and 10.0 µM) were detected using western blot analysis. The cell invasion rates were determined using transwell invasion assay and cell migration rates were determined using Wound-Healing assay. Results CD44 knockdown significantly reduced the cell invasion rates and cell migration rates in OCSLCs cells. DFOG downregulated the expression of CD44 as well as attenuated cell invasion and cell migration capabilities in OCSLCs. Moreover, knockdown of CD44 gene collaborated with DFOG to inhibit the ability of cell invasion and cell migration in OCSLCs. Conclusion DFOG inhibits cell invasion and cell migration capabilities by downregulating CD44 expression in OCSLCs .

导出引用

林佳芝, 刘会玲, 邓宇傲, 张晨, 宁映霞. DFOG下调CD44表达抑制SKOV3卵巢癌干样细胞体外侵袭和迁移[J]. 湖南师范大学学报医学版. 2023, 20(2): 16-21

LIN Jiazhi, LIU Huiling, DENG Yu'ao, ZHANG Chen, NING Yingxia. DFOG inhibits invasion and migration of SKOV3 ovarian cancer stem-like cells in vitro via down-regulating CD44 expression[J]. Journal of Hunan Normal University(Medical Science). 2023, 20(2): 16-21

中图分类号： R737.31

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