目的：研究长链非编码RNA（LncRNA）母系表达基因3（MEG3）及微小RNA-23b-3p（miR-23b-3p）与艾滋病患者特应性皮炎（atopic dermatitis,AD）严重程度的相关性。方法：选取2020年1月―2021年3月于本院感染科、皮肤科收治的65例艾滋病AD患者为研究对象（患病组）,根据特应性皮炎评分标准（SCORAD）分为重度组（9例）、中度组（36例）和轻度组（20例）;同时选取本院体检的65例健康人为对照组。采集所有入组对象静脉血,采用qRT-PCR法检测血清中LncRNA MEG3、miR-23b-3p的水平;Starbase数据库检索两者靶向结合位点;分析两者相关性以及两者水平分别与艾滋病AD患者SCORAD评分的相关性;采用Cox回归模型分析艾滋病AD患者预后不良的危险因素,按照艾滋病AD患者血清中LncRNA MEG3、miR-23b-3p表达水平进行分组,绘制生存曲线,并对生存曲线进行比较。结果：患病组血清中LncRNA MEG3表达水平较对照组明显降低;患病组中轻度组、中度组、重度组LncRNA MEG3表达水平差异有统计学意义;艾滋病AD患者血清中LncRNA MEG3水平、miR-23b-3p水平与SCORAD评分呈负相关;Starbase预测结果显示LncRNA MEG3、miR-23b-3p存在结合位点;艾滋病AD患者血清中LncRNA MEG3水平与miR-23b-3p水平呈正相关。单变量Log-rank 分析结果显示：LncRNA MEG3低表达、miR-23b-3p低表达与总生存期缩短显著相关。多变量Cox回归分析结果显示,LncRNA MEG3、miR-23b-3p表达水平降低是艾滋病AD患者预后不良的独立危险因素。结论：艾滋病AD患者血清中LncRNA MEG3、miR-23b-3p低表达与疾病的严重程度相关,且二者均为艾滋病AD患者预后不良的独立危险因素。

Objective Materaly expressed gene 3 ( MEG3), long noncodsne RNA(Lnc RNA ) and micrornA-23b-3p (miR-23b-3p) in relation to the severity of atopic dermatitis (AD) in AIDS patients. Methods Sixty-five AIDS patients with AD admitted to the Department of Infectious Diseases and Dermatology of our hospital from January 2020 to March 2021 were selected as research objects, recorded as the diseased group, and the severity of AIDS patients with AD was assessed on the basis of Scoring Atopic Dermatitis Index (SCORAD). They were recorded as severe group (9 cases), moderate group (36 cases) and mild group (20 cases), and 65 healthy people who underwent physical examination in our hospital were selected as control group. The serum levels of LncRNA MEG3 and miR-23b-3p were detected by qRT-PCR using equal volume blood of the diseased group and the control group, respectively. Starbase database was used to search the target binding sites of both. Pearson method was used to analyze the correlation between the two levels and SCORAD score of AIDS patients. Cox regression model was used to analyze the risk factors of poor prognosis in AIDS patients with AD. The groups were grouped according to the expression levels of LncRNA MEG3 and miR-23b-3p in serum of AIDS patients with AD (the mean expression levels of LncRNA MEG3 and miR-23b-3p in 65 AIDS patients with AD were taken as critical values). The survival curve was drawn and compared by Log-rank method. Results The expression levels of LncRNA MEG3 in serum of the infected group were significantly lower than those of the control group. There were statistically significant differences in the expression levels of LncRNA MEG3 and miR-23b-3p in mild group, moderate group and severe group. Correlation analysis results showed that the serum LncRNA MEG3 level and miR-23b-3p level of AIDS patients with AD were negatively correlated with SCORAD score. Starbase predicted that LncRNA MEG3 and miR-23b-3p had binding sites. There was a positive correlation between LncRNA MEG3 and miR-23b-3p in serum of AIDS patients with AD. Univariate Log-rank analysis showed that the low expression of LncRNA MEG3 and miR-23b-3p were significantly correlated with the shortened overall survival time. Cox regression model was used to analyze the risk factors affecting the prognosis of AIDS patients with AD. Multivariate Cox regression analysis showed that decreased expression level of LncRNA MEG3 and decreased expression level of miR-23b-3p were independent risk factors for poor prognosis in AIDS patients with AD. Conclusion The low expression of LncRNA MEG3 and miR-23b-3p in serum of AIDS patients with AD is related to the severity of the disease, and both are independent risk factors for poor prognosis in AIDS patients with AD.