NGF、转化生长因子&beta;1、成纤维细胞生长因子在骨折愈合中的作用效果分析

王裕祥; 王卫东; 杨应忠; 等

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湖南师范大学学报医学版 ›› 2018, Vol. 15 ›› Issue (3) : 95-98.

临床医学

NGF、转化生长因子β1、成纤维细胞生长因子在骨折愈合中的作用效果分析

王裕祥¹，王卫东¹，杨应忠²，李　军¹

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Analysis of the effect of NGF, transforming growth factor β1, fibroblast growth factor in fracture healing

Wang Yu-xiang¹, Wang Wei-dong¹, Yang Ying-zhong², Li Jun¹

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摘要

目的：探讨神经生长因子（NGF）、转化生长因子β1（TGF-β1）、成纤维细胞生长因子（FGF）在骨折愈合中的作用效果。方法：选取新西兰大白兔雄性30只，随机分为5组，包括生理盐水组、NGF组、TGF-β1组、FGF组、NGF+FGF+TGF-β1组，每组6只，所有动物建立胫骨骨折模型。生理盐水组、NGF组、TGF-β1组、FGF组、NGF+FGF+TGF-β1组术后在骨折端处分别注射生理盐水、NGF、TGF-β1、FGF以及NGF+FGF+TGF-β1，观察各组X线评分、骨密度及骨钙素水平。结果：术后14d、21d和28d，NGF组、TGF-β1组、FGF组、NGF+FGF+ TGF-β1组X线评分、骨密度及血清骨钙素均明显高于生理盐水组；术后21d和28d、NGF+FGF+TGF-β1组X线评分分别为（15.63±0.92）分和（16.01±0.93）分，明显高于NGF组、TGF-β1组和FGF组；术后14d、21d和28d NGF+FGF+TGF-β1组骨密度分别为（0.604±0.019）g/mm²、（0.613±0.021）g/mm²和（0.644±0.016）g/mm²，明显高于NGF组、TGF-β1组和FGF组；术后14d、21d和28d NGF+FGF+TGF-β1组血清骨钙素分别为（10.236±0.121）ng/ml、（11.924±0.093）ng/mL和（13.210±0.083）ng/mL，明显高于NGF组、TGF-β1组和FGF组。结论：NGF、TGF-β1和FGF 有一定的骨折愈合促进作用，三者具有较强的Ð同作用，能明显促进骨折愈合。

Abstract

Objective To investigate the effect of nerve growth factor (NGF), transforming growth factor β1(TGF- β1), fibroblast growth factor (FGF) in fracture healing. Methods Selected 30 male New Zealand rabbits, were randomly divided into 5 groups, including saline group, NGF group, TGF-β1 group, FGF group, NGF+FGF+TGF-β1 group, 6 rabbits in each group, tibial fracture model was established in all animals. The saline group, NGF group, TGF-β1 group, FGF group, NGF+FGF+TGF-β1 group after operation in the fracture site were injected saline, NGF, TGF-β1, FGF and NGF+FGF+TGF- β1, observed each group the X-ray score, bone mineral density and osteocalcin levels. Results Postoperative 14d, 21d and 28d, NGF group, TGF-β1 group, FGF group, NGF+FGF+ TGF-β1 group X-ray score, bone density and serum osteocalcin were significantly higher than those in the saline group; Postoperative 21d and 28d, NGF+FGF+TGF-β1 group X-ray scores were (15.63±0.92) and (16.01±0.93), significantly higher than that of NG group F, TGF-β1 group and FGF group; Postoperative 14d, 21d and 28d NGF+FGF+TGF- β1 groups bone density were (0.604±0.019) g/mm², (0.613±0.021) g/mm² and (0.644±0.016) g/mm², significantly higher than that of NGF group, TGF- β1 group and FGF group; Postoperative 14d, 21d and 28d NGF+ FGF+ TGF-β1 groups serum osteocalcin were (10.236±0.121) ng/ml, (11.924±0.093) ng/ml and (13.210 ±0.083) ng/ml, significantly higher than that of NGF group, TGF-β1 group and FGF group. Conclusion NGF, TGF-β1 and FGF has a certain role in promoting fracture healing, them has a strong synergistic effect, can obviously promote fracture healing.

关键词

神经生长因子

/ 转化生长因子&beta / 1 / 成纤维细胞生长因子 / 骨折愈合

Key words

nerve growth factor

/ transforming growth factor β1 / fibroblast growth factor / fracture healing

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王裕祥，王卫东，杨应忠，等.

NGF、转化生长因子β1、成纤维细胞生长因子在骨折愈合中的作用效果分析

[J]. 湖南师范大学学报医学版. 2018, 15(3): 95-98

Wang Yu-xiang, Wang Wei-dong, Yang Ying-zhong,et al.

Analysis of the effect of NGF, transforming growth factor β1, fibroblast growth factor in fracture healing

[J]. Journal of Hunan Normal University(Medical Science). 2018, 15(3): 95-98

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