目的：研究富亮氨酸重复1（leucine-rich-alpha-2-glycoprotein 1，LRG1）在人肝细胞癌（hepatocellular
carcinoma, HCC）中的表达情况，探讨其与HCC 的相关性。方法：选取 个肝癌细胞系及 0 对连续的新鲜冰冻
HCC 组织及其相应的癌旁非瘤肝组织标本，采取qRT-PCR 检测LRG1mRNA 的表达；Western blot 检测LRG1 的蛋
白表达。结果：肝癌细胞系中LRG1 的mRNA、蛋白的表达水平明显高于正常肝细胞系LO2，LRG1 的表达量与肝
癌细胞系的侵袭转移能力正相关，新鲜冰冻组织中LRG1 的mRNA、蛋白的表达水平明显高于相应的癌旁非瘤肝
组织。结论：LRG1 的高表达与HCC 的恶性程度密切相关，对其研究可用于研发新的靶向药物。
Abstract
Objective To investigate the expression of leucine-rich-alpha-2-glycoprotein 1(LRG1 ) in hepatocellular carcinoma
(HCC) Cell lines and tissue, and to explore its relationship with HCC progression. Methods qRT-PCR and Western
blot analysis were used to detect LRG1 mRNA and protein expression respectively in 6 hepatocellular carcinoma cell lines, 30
paired HCC and adjacent normal liver tissues. Results The levels of LRG1mRNA and protein expression hepatocellular carcinoma
cell lines mRNA, protein levels LRG1 significantly higher than normal liver cells L02, the of invasion and metastasis
of HCC cell lines positive correlation with LRG1 expression levels. The levels of LRG1 mRNA and protein expression in HCC
were significantly higher than those in adjacent liver tissues. Conclusion LRG1 overexpression has a significant correlation
with the progression of HCC, and it would designing new targeted drugs.
关键词
肝细胞癌 /
富亮氨酸重复蛋白1 /
侵袭转移
Key words
hepatocellular carcinoma /
leucine-rich repeat protein 1 /
invasion and metastasis;
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