目的：探讨薯蓣皂苷元(Dio) 干预对野百合碱(MCT) 诱导肺动脉高压(PAH) 大鼠影响。方法： 只大鼠
随机分为正常对照组、MCT 模型组、肺动脉高压薯蓣皂苷元干预组，检测平均肺动脉压力(mPAP)、右心指数(RV/
LV+S)、形态学指标、IL- 及凋亡指数，以观察Dio 干预对MCT 诱导PAH 大鼠的影响。结果：薯蓣皂苷元干预组
mPAP、右心指数、肺小动脉管壁厚度、肺小动脉胶原纤维含量、肺组织匀浆中IL- 水平明显低于MCT 模型组，而
凋亡指数明显高于MCT 模型组。结论：Dio 可能通过抑制肺血管重构、抑制炎症因子和促进肺血管平滑肌细胞凋
亡等途径，防治MCT 诱导的大鼠PAH。
Abstract
Objective Discuss the Effects of diosgenin intervention on monocrotaline inducing pulmonary artery hypertension
on rats. Methods Divide the 36 rats into normal control group, MCT model group and pulmonary artery hypertension diosgenin
intervention group. Detect the average pulmonary artery pressure (mPAP), right- cardiac index(RV/LV+S), morphology
index, IL-6 and apoptosis index to observe the effect of Dio intervention on MCT inducing PAH on rats. Results The mPAP,
right ventricle hypertrophy index, pulmonary arteriole wall thickness, collagenous fiber content in pulmonary arteriole and IL-6
level in pulmonary tissue homogenate of the diosgenin intervention group are significantly lower than those of the MCT model
group, while the apoptosis index is significantly higher than that of the MCT model group. Conclusion Dio may protect rats
against PAH induced by MCT, through inhibiting pulmonary vascular remodeling and inflammatory factor, and promoting pulmonary
vascular smooth muscle cell apoptosis.
关键词
薯蓣皂苷元 /
野百合碱 /
肺动脉高压 /
炎症 /
凋亡
Key words
diosgenin /
monocrotaline /
pulmonary arterial hypertension;inflammation /
apoptosis
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参考文献
［1］ Galiè N, Hoeper MM, Humbert M, et al. Guidelines for the diagnosis
and treatment of pulmonary hypertension［J］. Eur Respir J, 200 , ( ):
121 -12 .
［2］ Hassoun PM, Mouthon L, Barbera JA, et al. Inflammation, growth factors,
and pulmonary vascular remodeling［J］. J Am Coll Cardiol, 200 ,
5 (1): 10-1 .
［ ］ 孙波,刘文利.右心导管测定大鼠肺动脉压的实验方法［J］.中国医
学科学院学报,1 , ( ): - .
［ ］ Cracowski JL, Chabot F, Labarère J, et al. Proinflammatory cytokine
levels are linked to death in pulmonary arterial hypertension［J］. Eur
Respir J. 201 , ( ): 15- 1 .
［5］ Chaouat A, Savale L, Chouaid C, et al. Role for interleukin- in COPDrelated
pulmonary hypertension［J］. Chest. 200 ,1 ( ): – .
［ ］ 莫清华, 周应军, 曹建国, 等. 黄荆子乙酸乙酯提取物对HL- 0细
胞生长和凋亡的影响［J］. 湖南师范大学学报(医学版). 200 , 5(1):
1 -15.
［ ］ 刘慧, 朱俐俐, 李玲. 小檗碱对酒精所致心肌细胞凋亡的影响研究
［J］. 湖南师范大学学报(医学版)，201 , (01): - 1.
［ ］ Jung DH, Park HJ,Byun HE,et al.Diosgenin inhibits macrophage-derived
inflammatory mediators thrugh downregulation of CK2, JNK, NFkappaB
and AP-1 activation［ J］. Int ImmunopharmAcol, 2010, 10( ):
10 -105.
［ ］ Wang X, Cheng Y, Wang Y, et al. Dioscin induces cancer cell apoptosis
through elevated oxidative stress mediated by downregulation of peroxiredoxins
［ J］. Cancer Biolther, 2012, 1 (1 ):1 -1 .
［10］ Wu S, Xu H, Peng J, et al. Potent anti-inflammatory effect of dioscin
mediated by suppression of TNF-alpha-induced VCAM-1, ICAM-
1and EL expression via the NF-kappaB pathway［J］. Biochimie. 2015,
110(1): 2- 2.
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