目的：研究大肠癌组织及癌旁正常组织PTPRO 基因启动子甲基化状态及mRNA 表达情况，并探讨其
在大肠癌发生中的关系。方法：应用甲基化特异性PCR（MSP）和RT-PCR 分别检测 0 例大肠癌组织及癌旁正常
组织中PTPRO 基因启动子甲基化状态及mRNA 表达状态。结果：大肠癌组织中PTPRO 基因启动子甲基化率高于
癌旁正常组织，其甲基化样本中PTPRO 基因mRNA 表达明显受抑。结论：PTPRO 基因启动子甲基化在大肠癌发
生中起着重要作用，可能与大肠癌的发生、发展及预后有关。
Abstract
Objective Research the PTPRO Gene promoter methylation and the expression of PTPRO gene mRNA in
colorectal cancer tissue and normal tissue adjacent to carcinoma,and to discuss their relationship in colorectal cancer. Methods
Methylation specific PCR (MSP) and RT-PCR were use to detected PTPRO gene promoter methylation status and mRNA’s
expression in 30 cases of colorectal cancer tissue and in normal tissue adjacent to carcinoma. Results The PTPRO gene promoter
methylation rate of colorectal cancer tissues is higher than normal tissue adjacent to carcinoma,the PTPRO gene mRNA’s expression of the methylation samples is obviously inhibitive. Conclusion The PTPRO gene promoter methylation plays an
important role in colorectal cancer, and it may be related to the development and prognosis of colorectal cancer
关键词
大肠癌 /
PTPRO /
启动子 /
DNA 甲基化
Key words
colorectal cancer /
PTPRO /
promoter /
DNA methylation
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