DFMG对内皮细胞COX-2 表达和黏附功能的影响

张　婧; 张　勇; 刘诗炜; 黄　荷; 杨舒婷; 符晓华

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湖南师范大学学报医学版 ›› 2015, Vol. 12 ›› Issue (2) : 1-4.

基础医学

DFMG对内皮细胞COX-2 表达和黏附功能的影响

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The Effect of 7-Difluoromethoxy-5，4'-Dimethoxygenistein on COX-2 Expression and cell adhesion in HUVE-12 Cells

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摘要

目的:评估7-二氟甲氧基-5,4'-二甲氧基金雀异黄素(7-difluoromethoxy-5,4’-dimethoxygenistein,DFMG) 对损伤的人脐静脉内皮细胞( HUVE-12)中环氧化酶2 (cyclooxygenase-2, C0X-2)蛋白表达的影响及对HUVE-12细胞氧化损伤的保护作用。方法:肿瘤坏死因子-α（Tumor necrosis factor-α,TNF-α)诱导建立 HUVE-12损伤模型，不同浓度的DFMG对其进行诱导；流式细胞术(Flow Cytometry,FCM)检测细胞凋亡、Western blotting检测COX-2蛋白表达、蛋白定量分析法测定内皮细胞/单核细胞的黏附率、ELISA检测E-选择素、单核趋化蛋白-1 ( Monocyte chemoattractant protein-1, MCP-1)的释放。结果: DFMG 能拮抗损伤的内皮细胞活性的下降，并呈浓度依赖性，同时DFMG也能呈浓度依赖性地下调COX-2蛋白的表达、下调内皮细胞与单核细胞的黏附率。结论: DFMG可能通过减少COX-2蛋白表达水平而减少单核细胞/内皮细胞黏附作用，进一步减少炎症渗出，拮抗炎症反应，保护血管内皮细胞。

导出引用

张　婧, 张　勇, 刘诗炜, 黄　荷, 杨舒婷, 符晓华. DFMG对内皮细胞COX-2 表达和黏附功能的影响[J]. 湖南师范大学学报医学版. 2015, 12(2): 1-4

ZHANG 　Jing, ZHANG 　Yong, LIU Shi-Wei, HUANG 　He, YANG Shu-Ting, FU Xiao-Hua. The Effect of 7-Difluoromethoxy-5，4'-Dimethoxygenistein on COX-2 Expression and cell adhesion in HUVE-12 Cells[J]. Journal of Hunan Normal University(Medical Science). 2015, 12(2): 1-4

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