SHBG与妊娠合并糖尿病的相关性及其对滋养细胞生物学行为影响的研究

Abstract
Figure/Table
References
Related Citation (1)

Download: PDF (2614 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To assess the relationship between serum sex hormone-binding globulin (SHBG) concentrations and the risk of gestational diabetes mellitus (GDM) in early and midterm pregnancies and the effect of SHBG on the biological behavior of trophoblast cells. Methods During the period from March 2020 to March 2022, 65 GDM patients (case group) and 130 healthy pregnant women (control group) who were in early pregnancy (before 14 weeks) or middle pregnancy (14-28 weeks) were selected as the research objects. Serum SHBG level was measured by ELISA kit. In vitro, the trophoblast cell line HTR-8/SVneo was selected as the research object, which was divided into high glucose (HG) group and control group [normal glucose (NG) group]. HG group cells were transfected with SHBG plasmid or Vector. Cell proliferation, migration and invasion were investigated by cell counting kit, wound healing test and Transwell test. Results Serum SHBG concentrations were significantly higher in the GDM group compared to the control group. Also, pregnant women who subsequently developed GDM showed significantly higher serum SHBG concentrations in early pregnancy than controls. Logistic regression analysis showed an adjusted OR of 2.74(95%CI: 1.17-6.43) for the occurrence of GDM in the lowest tertile of serum SHBG. In addition, the multivariate adjusted OR for GDM was 1.49(95%CI: 1.05-2.12) for each standard deviation reduction in serum SHBG. Compared with the NG group, the HG group showed reduced expression of SHBG in HTR8/SVneo cells, decreased cell viability, and diminished cell migration and invasion. Compared with the HG+Vector group, the expression of SHBG in HTR8/SVneo cells was upregulated, cell viability was increased, and cell migration and invasion were enhanced in the HG+SHBG group. Conclusions The risk of GDM in pregnant women with low serum SHBG concentration increases significantly, and the up-regulation of SHBG promotes the proliferation and migration of trophoblasts in HG background.

Key words： sex hormone binding globulin early pregnancy second trimester pregnancy gestational diabetes trophoblast

Received: 03 November 2022

PACS:

R714.256

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WANG Min
	ZHANG Xiaocai
	DUAN Bei
	PEI Qiaoli
	XU Shan
	YUAN Ningxia
	ZHU Lihong
	LI Wenting

Cite this article:

WANG Min,ZHANG Xiaocai,DUAN Bei等. Correlation of SHBG with gestational combined diabetes and its effect on the biological behavior of trophoblast cells[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(2): 40-45.

URL:

http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2023/V20/I2/40

[1] 王娜, 邓泽群, 丁焱, 等. 孕期膳食模式对妊娠期糖尿病发生风险的影响[J]. 中华糖尿病杂志, 2021, 13(9) : 865-871.
[2] 陈媛, 张家佳, 肖雄, 等. 妊娠期糖尿病患者胎盘组织和血清Gal-13, TBX5, FGF19表达水平与妊娠结局的关系[J]. 实用妇产科杂志, 2021, 37(8) : 624-630.
[3] 徐庆英, 王健. 胎盘组织中GLUT, IRS, SHBG与妊娠期糖尿病相关性[J]. 中国计划生育学杂志, 2021, 29(5) : 1025-1028.
[4] O’Reilly M W, Glisic M, Kumarendran B, et al. Serum testosterone, sex hormone‐binding globulin and sex-specific risk of incident type 2 diabetes in a retrospective primary care cohort[J]. Clin Endocrinol, 2019, 90(1) : 145-154.
[5] Zhang T, Du T, Li W, et al.Sex hormone-binding globulin levels during the first trimester may predict gestational diabetes mellitus development[J]. Biomark Med, 2018, 12(3) : 239-244.
[6] Liu W, Huang Z, Tang S, et al.Changes of serum sex hormone-binding globulin, homocysteine, and hypersensitive CRP levels during pregnancy and their relationship with gestational diabetes mellitus[J]. Gynecol Obstet Invest, 2021, 86(1-2) : 193-199.
[7] 吴红花. 重视妊娠期高血糖分类及妊娠期糖尿病诊断标准[J]. 中华医学杂志, 2022, 102(6) : 389-392.
[8] Zhang L, Zeng M, Tang F, et al.Circ-PNPT1 contributes to gestational diabetes mellitus (GDM) by regulating the function of trophoblast cells through miR-889-3p/PAK1 axis[J]. Diabetol Metab Syndr, 2021, 13(1) : 58.
[9] Wang X, Chi X, Feng C, et al.Sex hormone-binding globulin regulates the activity of the ERK pathway in the placentas of patients with gestational diabetes mellitus[J]. Biochem Biophys Res Commun, 2020, 532(4) : 613-619.
[10] Chi X, Feng C, Wang X, et al.Sex hormone-binding globulin regulates glucose metabolism in human placental trophoblasts via cAMP/PKA/CREB1[J]. J Obstet Gynaecol Res, 2020, 46(11) : 2340-2346.
[11] Qu X, Donnelly R.Sex hormone-binding globulin (SHBG) as an early biomarker and therapeutic target in polycystic ovary syndrome[J]. Int J Mol Sci, 2020, 21(21) : 8191.
[12] Siddiqui K, George T P, Joy S S, et al.Association of sex hormone binding globulin with gestational age and parity in gestational diabetes mellitus[J]. J Matern Fetal Neonatal Med, 2022, 35(8) : 1433-1438.
[13] Saade G R, Boggess K A, Sullivan S A, et al. Development and validation of a spontaneous preterm delivery predictor in asymptomatic women[J]. Am J Obstet Gynecol, 2016, 214 (5) : 633. e1-633. e24.
[14] Chi X, Feng C, Wang X, et al.Sex hormone-binding globulin regulates glucose metabolism in human placental trophoblasts via cAMP/PKA/CREB1[J]. J Obstet Gynaecol Res, 2020, 46(11) : 2340-2346.
[15] 朱冰, 牛爱琴, 王坤. 内质网应激对妊娠期糖尿病滋养层细胞的影响[J]. 中华生物医学工程杂志, 2021, 27(5) : 494-500.
[16] Ke W, Chen Y, Zheng L, et al.miR-134-5p promotes inflammation and apoptosis of trophoblast cells via regulating FOXP2 transcription in gestational diabetes mellitus[J]. Bioengineered, 2022, 13(1) : 319-330.