Infection situation and S gene mutation of occult hepatitis B virus infection among natural population in Changsha from 2018 to 2021

WANG Yuanyi; ZHANG Biming; HOU Zhengli; YANG Qian; QUE Zhengshu

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Journal of Hunan Normal University(Medical Science) ›› 2023, Vol. 20 ›› Issue (4) : 60-64.

Infection situation and S gene mutation of occult hepatitis B virus infection among natural population in Changsha from 2018 to 2021

WANG Yuanyi, ZHANG Biming, HOU Zhengli, YANG Qian, QUE Zhengshu

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Abstract

Objective To research the prevalence of the occult hepatitis B virus infection (OBI) among the natural population in Changsha from 2018 to 2021 and to explore the genotype distribution of virus strain and the mutation of amino acid sequence in S region , so as to provide an epidemiological and molecular biological basis for exploring the prevention and control of OBI in this region. Methods A total of 68, 513 serum samples from september 2018 to december 2021 were collected, Among them, OBI samples were screened, yield and their epidemiological characteristics were analyzed. Confirmed OBI samples will be tested for serology and high-susceptibility viral load testing, and were genetically amplified in the S region by nested PCR amplification and sequenced, HBV gentyping and amino acid mutation analysis in the S-region were performed using MEGA6.0 software. Results 27 OBI samples were confirmed , the detection rate of OBI was 0.039%; 5 OBI serological patterns were found, anti-HBc positive rate was 88.90% in all samples; HBV DNA nucleic acid quantitative ranged from 9.0 to 191.0(IU/mL) (median value of 19.0); 8 samples of those OBI donors were amplified for S region, all those are B genotype, which was shown amino acid mutation in MHR region of S gene, especially in HBV“a”epitope. Conclusion The OBI rate of natural population in Changsha is at low level in china (0.0106％-2.71％) from 2018 to 2021; The genotype of HBV is mainly type B, The mutation in MHR region of S gene, especially in HBV “a”epitope may be one of the important factors to cause OBI.

Key words

occult hepatitis B virus / natural population / genotype of HBV / mutation analysis

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WANG Yuanyi, ZHANG Biming, HOU Zhengli, YANG Qian, QUE Zhengshu. Infection situation and S gene mutation of occult hepatitis B virus infection among natural population in Changsha from 2018 to 2021[J]. Journal of Hunan Normal University(Medical Science). 2023, 20(4): 60-64

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