血清LncRNA MEG3、miR-23b-3p与艾滋病患者特应性皮炎严重程度的相关性研究

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摘要 目的：研究长链非编码RNA（LncRNA）母系表达基因3（MEG3）及微小RNA-23b-3p（miR-23b-3p）与艾滋病患者特应性皮炎（atopic dermatitis,AD）严重程度的相关性。方法：选取2020年1月―2021年3月于本院感染科、皮肤科收治的65例艾滋病AD患者为研究对象（患病组）,根据特应性皮炎评分标准（SCORAD）分为重度组（9例）、中度组（36例）和轻度组（20例）;同时选取本院体检的65例健康人为对照组。采集所有入组对象静脉血,采用qRT-PCR法检测血清中LncRNA MEG3、miR-23b-3p的水平;Starbase数据库检索两者靶向结合位点;分析两者相关性以及两者水平分别与艾滋病AD患者SCORAD评分的相关性;采用Cox回归模型分析艾滋病AD患者预后不良的危险因素,按照艾滋病AD患者血清中LncRNA MEG3、miR-23b-3p表达水平进行分组,绘制生存曲线,并对生存曲线进行比较。结果：患病组血清中LncRNA MEG3表达水平较对照组明显降低;患病组中轻度组、中度组、重度组LncRNA MEG3表达水平差异有统计学意义;艾滋病AD患者血清中LncRNA MEG3水平、miR-23b-3p水平与SCORAD评分呈负相关;Starbase预测结果显示LncRNA MEG3、miR-23b-3p存在结合位点;艾滋病AD患者血清中LncRNA MEG3水平与miR-23b-3p水平呈正相关。单变量Log-rank 分析结果显示：LncRNA MEG3低表达、miR-23b-3p低表达与总生存期缩短显著相关。多变量Cox回归分析结果显示,LncRNA MEG3、miR-23b-3p表达水平降低是艾滋病AD患者预后不良的独立危险因素。结论：艾滋病AD患者血清中LncRNA MEG3、miR-23b-3p低表达与疾病的严重程度相关,且二者均为艾滋病AD患者预后不良的独立危险因素。

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	李元香
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	莫代芬
	吴二英
	钟华芬
	黄彬
	潘亚娇

关键词 ：长链非编码RNA母系表达基因3, 微小RNA-23b-3p, 艾滋病, 特应性皮炎, 病情严重程度, 预后分析

Abstract：Objective Materaly expressed gene 3 ( MEG3), long noncodsne RNA(Lnc RNA ) and micrornA-23b-3p (miR-23b-3p) in relation to the severity of atopic dermatitis (AD) in AIDS patients. Methods Sixty-five AIDS patients with AD admitted to the Department of Infectious Diseases and Dermatology of our hospital from January 2020 to March 2021 were selected as research objects, recorded as the diseased group, and the severity of AIDS patients with AD was assessed on the basis of Scoring Atopic Dermatitis Index (SCORAD). They were recorded as severe group (9 cases), moderate group (36 cases) and mild group (20 cases), and 65 healthy people who underwent physical examination in our hospital were selected as control group. The serum levels of LncRNA MEG3 and miR-23b-3p were detected by qRT-PCR using equal volume blood of the diseased group and the control group, respectively. Starbase database was used to search the target binding sites of both. Pearson method was used to analyze the correlation between the two levels and SCORAD score of AIDS patients. Cox regression model was used to analyze the risk factors of poor prognosis in AIDS patients with AD. The groups were grouped according to the expression levels of LncRNA MEG3 and miR-23b-3p in serum of AIDS patients with AD (the mean expression levels of LncRNA MEG3 and miR-23b-3p in 65 AIDS patients with AD were taken as critical values). The survival curve was drawn and compared by Log-rank method. Results The expression levels of LncRNA MEG3 in serum of the infected group were significantly lower than those of the control group. There were statistically significant differences in the expression levels of LncRNA MEG3 and miR-23b-3p in mild group, moderate group and severe group. Correlation analysis results showed that the serum LncRNA MEG3 level and miR-23b-3p level of AIDS patients with AD were negatively correlated with SCORAD score. Starbase predicted that LncRNA MEG3 and miR-23b-3p had binding sites. There was a positive correlation between LncRNA MEG3 and miR-23b-3p in serum of AIDS patients with AD. Univariate Log-rank analysis showed that the low expression of LncRNA MEG3 and miR-23b-3p were significantly correlated with the shortened overall survival time. Cox regression model was used to analyze the risk factors affecting the prognosis of AIDS patients with AD. Multivariate Cox regression analysis showed that decreased expression level of LncRNA MEG3 and decreased expression level of miR-23b-3p were independent risk factors for poor prognosis in AIDS patients with AD. Conclusion The low expression of LncRNA MEG3 and miR-23b-3p in serum of AIDS patients with AD is related to the severity of the disease, and both are independent risk factors for poor prognosis in AIDS patients with AD.

Key words： long non-coding RNA maternal expression gene 3 microrna-23b-3p AIDS atopic dermatitis the severity of the disease prognostic analysis

收稿日期: 2022-12-30

中图分类号:

R759

基金资助:湖南公共卫生联盟科研专项（KY2022-005）

通讯作者: 潘亚娇,E-mail：tuotuo816@163.com

引用本文:

李元香, 郭艳艳, 莫代芬, 吴二英, 钟华芬, 黄彬, 潘亚娇. 血清LncRNA MEG3、miR-23b-3p与艾滋病患者特应性皮炎严重程度的相关性研究[J]. journal1, 2023, 20(2): 104-108. LI Yuanxiang, GUO Yanyan, MO Daifen, WU Erying, ZHONG Huafen, HUANG Bin, PAN Yajiao. Study on the correlation between serum LncRNA MEG3 and miR-23b-3p and the severity of atopic dermatitis in AIDS patients. journal1, 2023, 20(2): 104-108.

链接本文:

http://yxb.hunnu.edu.cn/CN/ 或 http://yxb.hunnu.edu.cn/CN/Y2023/V20/I2/104

[1] 裴小平, 谷梅, 陈文静, 等. 儿童特应性皮炎严重度与25羟维生素D, 总IgE及IL-4水平的相关性研究[J]. 皮肤性病诊疗学杂志, 2020, 27(4): 227-231.
[2] Kim J, Kim BE, Leung DYM.Pathophysiology of atopic dermatitis: Clinical implications[J]. Allergy Asthma Proc, 2019, 40(2): 84-92.
[3] 李晓静, 黄刚莉, 王露, 等. 外周血白细胞介素-13与干扰素-γ在儿童特应性皮炎中的发病机制研究[J]. 现代生物医学进展, 2020, 20(13): 2533-2537.
[4] Guo FX, Wu Q, Li P, et al.The role of the LncRNA-FA2H-2-MLKL pathway in atherosclerosis by regulation of autophagy flux and inflammation through mTOR-dependent signaling[J]. Cell Death Differ, 2019, 26(9): 1670-1687.
[5] 陈宏坤, 张大森. 先天性巨结肠患者血清miR-223与炎性因子水平及其临床意义研究[J]. 临床小儿外科杂志, 2021, 20(04): 359-364.
[6] Zhong Y, Qin K, Li L, et al.Identification of Immunological Biomarkers of Atopic Dermatitis by Integrated Analysis to Determine Molecular Targets for Diagnosis and Therapy[J]. Int J Gen Med, 2021, 14: 8193-8209.
[7] 中华中医药学会防治艾滋病分会. 艾滋病皮肤瘙痒协同治疗专家共识[J]. 中医学报, 2020, 35(04): 689-693.
[8] 程浩. 艾滋病相关炎症性皮肤粘膜疾病[J]. 皮肤科学通报, 2019, 36(03): 374-381, 387.
[9] 艾滋病常见病证辨证施治技术指导原则[J]. 中医学报, 2014, 29(04): 463-465.
[10] 张学军. 皮肤性病学 (第8版) [M]. 北京: 人民卫生出版社, 2013: 111-113.
[11] 李迎, 张吉林, 宋玉国. 特应性皮炎患者外周血microRNA-155和CD4+T细胞检测的临床意义[J]. 中国免疫学杂志, 2015, 31(12): 1679-1682.
[12] Simpson EL, Flohr C, Eichenfield LF, et al.Efficacy and safety of lebrikizumab (an anti-IL-13 monoclonal antibody) in adults with moderate-to-severe atopic dermatitis inadequately controlled by topical corticosteroids: A randomized, placebo-controlled phase II trial (TREBLE)[J]. J Am Acad Dermatol, 2018, 78(5): 863-871.
[13] 李蒙娜. 长链非编码RNA在紫外线诱导皮肤光损伤过程中的功能机制研究[D]. 北京: 北京协和医学院, 2020.
[14] 李婷婷, 王艺萌, 张春雷. 长链非编码RNA在T细胞相关性皮肤病中的作用[J]. 中国皮肤性病学杂志, 2022, 36(01): 10-16.
[15] 王蓉, 袁涛. 长链非编码RNA在恶性黑色素瘤侵袭和转移中作用的研究进展[J]. 中国肿瘤生物治疗杂志, 2021, 28(03): 311-316.
[16] 叶利芳, 舒晓明, 左瑜, 等. miR-23b-3p在皮肌炎血清中的表达与临床意义[J]. 中日友好医院学报, 2018, 32(5): 271-274.
[17] 朱连海, 何长生, 申建伟, 等. LncRNA MEG3在病毒性毛细支气管炎患儿外周血单个核细胞和痰液中表达及意义[J]. 河北医学, 2020, 26(6): 946-951.
[18] 张欣, 郑美娟, 徐元宏. miRNA-143对人鼻咽癌细胞CNE-2Z增殖、迁移和侵袭的影响[J]. 安徽医科大学学报, 2017, 52(4): 480-483.
[19] Li C, Bai Y, Liu H, et al.Comparative study of microRNA profiling in keloid fibroblast and annotation of differential expressed microRNAs[J]. Acta Biochim Biophys Sin (Shanghai), 2013, 45(8): 692-699.
[20] Kozubek J, Ma Z, Fleming E, et al.In-depth characterization of microRNA transcriptome in melanoma[J]. PLoS One, 2013, 8(9): e72699.