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Quick Search     Advanced Search   2024 Vol.  21 No.  2 Published: 2024-04-25 Basic Medicine Clinical Medicine Nursing Science Invited Review Laboratory Medicine Invited Review 1 LI Pingping, JIANG Xinyue, HU Xinyue, LI Yawen, SONG Yixuan, ZHANG Mingliang Metabolic Regulation on Myelination and Remyelination In the central nervous system (CNS), oligodendrocyte precursor cells (OPCs) proliferate, migrate, and differentiate into oligodendrocytes (OLs), which subsequently form myelin structure that ensheathes the axons of neurons. Myelin enables fast saltatory impulse propagation, provides energy and nutrition for neurons, and facilitates the establishment and maintenance of cognition. Notably, the (re) generation of myelin requires substantial energy consumption. Glucose, the preferred energy resource for many biological processes, plays a vital role in (re) myelination. Thus, we review the recent progress in the regulation of glucose metabolism on myelination to extend our understanding of myelination and to provide insights into the potential strategy for treating diseases of demyelination and neurodegeneration. 2024 Vol. 21 (2): 1-7 [Abstract] ( 97 ) [HTML 1KB] [PDF 2347KB] ( 729 ) Basic Medicine 8 WANG Yi, LIU Chongyang, DUAN Huahong, YU Xing Canagliflozin inhibits galectin-3-mediated proliferation and migration of intrahepatic cholangiocarcinoma cells Objective To study the role of canagliflozin in inhibiting galectin-3-mediated proliferation and migration of intrahepatic cholangiocarcinoma cells. Methods TIMER2.0, GEPIA2 and R2 databases were used to analyze the expression of Gal-3 in cholangiocarcinoma and whether it had any effect on the overall survival rate of cholangiocarcinoma patients. The expression of Gal-3 in intrahepatic cholangiocarcinoma tissues and paracarcinoma tissues was detected by immunohistochemistry. Molecular docking was used to analyze the binding of canagliflozin to Gal-3. Cell experiments were performed with the intrahepatic cholangiocarcinoma cell line HUCCT1, and cells were treated with different concentrations of canagliflozin for 24h and 48h, and the effect of canagliflozin on cell survival rate was detected by CCK8. After treating cells with 16μM canagliflozin for 24h, the effect of canagliflozin on the proliferation and migration of cells was analyzed by using EdU Fluorescent Labeling and the Wound-Healing Assay. Results Bioinformatics Analysis results showed that Gal-3 was significantly highly expressed in cholangiocarcinoma, and the immunohistochemical results using intrahepatic cholangiocarcinoma tissues and paracarcinoma tissues also showed that the expression of Gal-3 was significantly increased in intrahepatic cholangiocarcinoma, and that the high expression of Gal-3 was significantly correlated with the overall survival rate of the cholangiocarcinoma patients. The results of the molecular docking study revealed that canagliflozin is capable of recognizing Gal-3 at a novel site instead of the conventional carbohydrate binding site. As the concentration of canagliflozin treatment increased and the treatment time was prolonged, the cell survival rate decreased. After treating HUCCT1 cells with 16μM canagliflozin for 24 h, the results of EdU Fluorescent Labeling showed that the proliferative ability of the treated group was significantly lower than that of the control group. The results of the Wound-Healing Assay showed that the cell migration rate of the treated group was significantly lower than that of the control group. Conclusion Canagliflozin inhibits the proliferation and migration of intrahepatic cholangiocarcinoma cells by binding to Gal-3. 2024 Vol. 21 (2): 8-14 [Abstract] ( 83 ) [HTML 1KB] [PDF 4746KB] ( 696 ) 15 YU Tingzi, DING Cong, TANG Yongyi, LI Zhuan SIRT7 deficiency in hepatic cell mediates PAI-1 secretion, activating hepatic stellate cell and promoting liver fibrosis Background The population affected by liver fibrosis is extensive, yet treatment options are lacking. SIRT7, a member of the sirtuin family of histone deacetylases, is involved in various physiological processes such as cellular aging, metabolism, and inflammatory responses. It has been reported that SIRT7 plays crucial roles in many liver diseases, including hepatocellular carcinoma and non-alcoholic fatty liver disease, but its function in liver fibrosis remains unclear. Methods A liver fibrosis model was established by alternate-day intraperitoneal injection of CCl4 in both wild-type mice and hepatocyte SIRT7 knockout mice, followed by assessment of injury and fibrosis phenotypes. Subsequently, primary cells were used for in vitro co-culture experiments, combined with array analysis to further determine the mediators of hepatic stellate cell (HSC) activation affected by SIRT7 deficiency. Conclusion SIRT7 deficiency exacerbated CCl4-induced liver injury and fibrosis phenotypes. Further research indicated that SIRT7 deficiency mediated differential activation of hepatic stellate cells (HSCs) through hepatocyte PAI-1 secretion, thereby promoting liver fibrosis. Our results suggested that SIRT7-mediated signaling plays a significant role in liver injury and fibrosis, and therapeutic targeting SIRT7 may be an effective approach for treating liver fibrosis. 2024 Vol. 21 (2): 15-20 [Abstract] ( 84 ) [HTML 1KB] [PDF 3061KB] ( 656 ) 21 WANG Hanqin, KANG Huimin, ZHOU Ke, LI Rou, CHEN Shi Identification of inflammation related key genes in three mouse lung diseases based on experimental studies and bioinformatics Objective RNA sequencing and bioinformatics methods were utilized to identify key genes associated with mouse silicosis, idiopathic pulmonary fibrosis (IPF), and chronic obstructive pulmonary disease (COPD). This research aims to establish a novel experimental foundation for diagnosing and treating chronic inflammatory lung diseases. Methods A mouse model of silicosis was established and validated, then RNA sequencing was conducted on the lung tissues of silicosis mice. Differentially expressed genes (DEGs) were identified using the limma package, volcano and heat maps were generated with the ggplot package. Common DEGs in silicosis, IPF, and COPD were identified using a Venn diagram. A protein-protein interaction (PPI) network was constructed using the STRING database to identify key genes among the DEGs. The expression of key genes in the lung tissues of silicosis mice was validated through qPCR assays, and the expression of key genes in IPF and COPD mice was validated using the GSE218997 and GSE76205 datasets. Results A mouse silicosis model was established and RNA sequencing of mouse lung tissue was performed, in conjunction with datasets from IPF and COPD mice, a total of 8 common DEGs were identified across the three diseases. The qPCR results revealed a significant increase in Cxcl10, MMP12, and Lcn2 in the lung tissue of silicosis mice, while no significant change was observed in Ctss levels. In the validation sets of IPF and COPD, Ctss, Cxcl10, MMP12, and Lcn2 all showed high expression. Conclusion Cxcl10, MMP12, and Lcn2 are potential inflammation related key genes for mouse silicosis, IPF, and COPD. 2024 Vol. 21 (2): 21-26 [Abstract] ( 108 ) [HTML 1KB] [PDF 5929KB] ( 666 ) 27 YANG Yingying, JIANG Hua, YAN Juan The Mechanism of MiR-181a Overexpression Involved in Regulating Treg Cells Involved in Allergic Rhinitis Objective To explore the mechanism of miR-181a overexpression in regulating Treg cells to participate in allergic rhinitis (AR). Methods 20 children with AR and 20 children with similar age and without allergic history were selected as the research objects. Pearson correlation analysis was used to analyze the relationship between the number of Treg and the expression of miR-181a. The Tregs were purified from PBMCs in AR children. The miR-181a mimics and inhibitors were transfected into Tregs. The function of Tregs were evaluated by flow cytometry and ELISA assay. Mice were divided into 7 groups, with 10 mice in each group: control group, AR group, AR+ negative control group, AR+miR-181a simulation group, AR+miR-181a inhibitor group, AR+miR-181a simulation+recombinant OPN protein group, and AR+miR-181a simulation +OPN lentivirus shRNA group. AR models were established using ovalbumin, after which the functional role of miR-181a in AR was determined using gain- and loss-of-function approaches. Results Compared with the control group, the number of Treg and the expression of miR-181a in the PBMCs of patients with AR were significantly decreased. Pearson correlation analysis showed that the number of Treg in PBMCs was positively correlated with the expression of miR-181a (R2=0.335, P<0.001). MiR-181a mimics promoted the proliferation of Tregs and upregulated the mRNA expression of IL-10 and TGF-β. Luciferase assay showed that miR-181a directly targeted the 3＇UTR of OPN. In animal experiments, compared with AR and AR + miR-NC group, the inflammation in AR + miR-181a simulation group was reduced, and the inflammation in AR + miR-181a inhibitor group was more serious. The recombinant OPN protein significantly reversed the anti-inflammatory effect of miR-181a mimic. In addition, Treg cells in AR group decreased significantly, and miR-181a mimic significantly increased the number of Treg cells in AR mice, while the recombinant OPN protein significantly reversed the increase of Treg cells induced by miR-181a mimic. Conclusion Upregulation of miR-181a significantly decreases the expression of OPN, and subsequently decreases eosinophils and enhances Treg function, by which it contributes to reduce the airway inflammation in the pathogenesis of AR. 2024 Vol. 21 (2): 27-32 [Abstract] ( 124 ) [HTML 1KB] [PDF 5158KB] ( 654 ) 33 SUN Jiyou, ZHAI Zhiwei, REN Hongjie, QIAO Luming, DU Hengzhou, LI Xiaojuan To investigate the effects of sleeve gastrectomy on cognitive function in offspring of obese rats based on autophagy mediated by BDNF/TrkB pathway Objective To investigate the effects of sleeve gastrectomy (SG) on cognitive function in offspring of obese rats based on autophagy mediated by BDNF/TrkB pathway. Methods Thirty clean female SD rats were selected and divided into control group, obesity group and SG group. The rats in each group were mated with healthy male rats fed ordinary diet. After the offspring of the mating rats in each group grew up to 3 weeks old. The cognitive function of offspring was detected by Morris water maze experiment. Nissl and H&E staining were used to detect the number of Nissl bodies in the hippocampus of rat offspring. The contents of total cholesterol (TG), triglyceride (TC) and free fatty acid (FFA) in serum of rat offspring in each group were detected by the kit. The protein levels of BDNF, TrkB, Beclin1 and LC3Ⅱ in offspring hippocampal tissues were detected by Western blotting, the levels of BDNF and TrkB in hippocampal tissues were detected by immunohistochemical staining, and the number of autophagosomes was detected by transmission electron microscopy. Results Compared with the control group, the progeny escape latency of the obese rats was prolonged and the frequency of crossing the platform was reduced, the number of Nissl bodies in hippocampus decreased, and the contents of TG, TC and FFA in serum increased, the protein levels of BDNF, TrkB, Beclin1 and LC3Ⅱ in hippocampus decreased, the number of autophagosomes decreased; Compared with the obese group, the offspring of SG group had shorter escape latency and increased platform crossing times, the number of Nissl bodies in hippocampus increased, and the contents of TG, TC and FFA in serum decreased, the protein levels of BDNF, TrkB, Beclin1 and LC3Ⅱ in hippocampus were increased, the number of autophagosomes increased. Conclusion SG can improve the cognitive function of offspring of obese rats by activating autophagy mediated by BDNF/TrkB pathway. 2024 Vol. 21 (2): 33-38 [Abstract] ( 105 ) [HTML 1KB] [PDF 8614KB] ( 620 ) 39 YU Bo, PENG Peng, HUANG Liang, BAO Liwei, WANG Zihan, WANG Weimin, ZHANG Xia Clinical significance of USP1 expression in ovarian cancer and its effect on malignant phenotype of ovarian cancer cells Objective To investigate the expression of ubiquitin-specific peptidase 1(USP1) in ovarian cancer and its correlation with clinicopathologic features of patients, as well as to explore the impact of USP1 on the malignant phenotype of ovarian cancer cells. Methods The expression level of USP1 in ovarian cancer patients and its relationship with clinical stage were analyzed by bioinformatics. Immunohistochemistry was used to detect the expression of USP1 in ovarian cancer patients and normal ovarian tissues, and to analyze the relationship between its expression level and the clinical characteristics of the patients. The SKOV3 cell line overexpressing USP1 was constructed by lentiviral infection, and the cell proliferation and clone formation abilities were detected by CCK-8 and clone formation assay, while the migration and invasion abilities were detected by Transwell assay. Results The expression level of USP1 in ovarian cancer tissues was significantly higher than that in normal ovarian tissues, and was correlated with the pathological type, peritoneal metastasis and the size or invasion degree of tumor in situ, but was not correlated with the expression levels of Ki67, ER, PR and P53. The results of in vitro experiments showed that USP1 promoted the proliferation, colony formation, migration and invasion ability of ovarian cancer cells. Conclusion USP1 expression was enhanced in ovarian cancer tissues and correlated with patients＇ pathological type, peritoneal metastasis, and clinical stage, and USP1 promoted the progression of the malignant phenotype of ovarian cancer cells. 2024 Vol. 21 (2): 39-44 [Abstract] ( 89 ) [HTML 1KB] [PDF 3210KB] ( 765 ) Clinical Medicine 45 ZHAO Min, YANG Yuying, GAO Xiaoxia, LI Jiming Effect of Hif-1α/Vegf signal pathway activation on the condition of patients with basal ganglia hemorrhage Objective To investigate the relationship between the expression of hypoxia-inducible factor 1α (HIF-1α) /vascular endothelial growth factor (VEGF) and the condition and prognosis of basal ganglia hemorrhage. Methods A total of 106 patients with basal ganglia hemorrhage from January 2019 to January 2022 were selected as the research subjects. The concentrations of serum HIF-1α and VEGF were measured by sandwich enzyme-linked immunosorbent assay with double antibodies. The associations between serum HIF-1α and VEGF concentrations and early neurological deterioration and poor prognosis 3 months after injury were analyzed by multivariate logistic regression, and the predictive power of the variables was assessed by receiver operating characteristic curve. Results The serum HIF-1α and VEGF concentrations at admission were significantly positively correlated with the admission NIHSS score (r=0.6267, 0.8495, both P<0.001). With the increase of baseline hematoma size, the serum HIF-1α and VEGF concentrations were significantly increased after acute ICH (r=0.5352, 0.6418, both P<0.001). Serum HIF-1α concentration (OR=4.054, 95%CI: 1.368-12.009; P=0.012) and serum VEGF concentration (OR=1.821, 95%CI: 1.119-2.965; P=0.016) were independent of END after acute ICH predictor. Serum HIF-1α concentration (OR=4.721, 95%CI: 1.662-13.407; P=0.014) and serum VEGF concentration (OR=2.198, 95%CI: 1.204-4.013, P=0.010) were associated with worse prognosis after acute ICH independent predictors. Serum HIF-1α concentration predicted early neurological deterioration (AUC=0.746, 95%CI: 0.6572-0.8178, P<0.001) and 3-month prognosis (AUC=0.7744, 95%CI: 0.7072-0.8416; P<0.001) was better than the serum VEGF concentrations (AUC=0.6882, 95%CI: 0.6113-0.7650; P<0.001 and AUC=0.7025, 95%CI: 0.6270-0.7780; P<0.001). Conclusion Serum HIF-1α and VEGF concentrations are independently associated with END and poor prognosis 3 months after ICH. In addition, the prognostic value of serum HIF-1α concentration is better than that of serum VEGF. 2024 Vol. 21 (2): 45-50 [Abstract] ( 86 ) [HTML 1KB] [PDF 2635KB] ( 642 ) 51 ZHANG Chunmei, SONG Jie, WANG Jinxiang Morphological changes of peripheral blood cells in elderly patients with COPD and pulmonary infection and their prognostic value Objective To investigate the morphological changes of peripheral blood cells in elderly patients with chronic obstructive pulmonary disease (COPD) and pulmonary infection and its prognostic value. Methods 140 patients with COPD and pulmonary infection admitted to our hospital from January 2020 to October 2022 were selected. Peripheral blood cell morphology, lung function [Percentage of forced expiratory volume in the 1s to the expected value (FEV1%pred), forced expiratory volume in the 1s/forced vital capacity (FEV1/FVC) ] and infection-related inflammatory factors [serum C-reactive protein (CRP), touchglobin (HPT), soluble leukocyte differentiation antigen 14(sCD14), and membranin A1(ANXA1) ] were detected on the day after admission. After 30 days of follow-up, the patients were divided into survival group (n=121) and death group (n=19) according to prognosis. Peripheral blood cell morphological examination results (abnormal white blood cell morphology, red blood cell morphology and platelet count) and peripheral blood infection-related inflammatory factors were compared between the two groups, and peripheral blood cell morphology and infection-related inflammatory factors were compared in patients with different COPD grades. The correlation of peripheral blood cell morphology with lung function and infection-related inflammatory factors was analyzed, and the prognostic value of peripheral blood cell morphology in elderly patients with COPD and pulmonary infection was analyzed. Results The comparison of abnormal rates of white blood cell morphology and red blood cell morphology between the survival group and the death group showed that the survival group was lower than the death group; The comparison of FEV1% pred, FEV1/FVC, CRP, sCD14, ANXA1, and HPT between the survival group and the death group showed that the levels of FEV1% pred, FEV1/FVC in the survival group were higher than those in the death group, while the levels of CRP, sCD14, ANXA1, and HPT were lower than those in the death group; The difference in the rates of abnormal white blood cell morphology and abnormal red blood cell morphology among COPD grade I, II, and III patients is statistically significant; Comparison of FEV1% pred, FEV1/FVC, CRP, sCD14, ANXA1, and HPT levels in COPD grade I, II, and III patients; the FEV1%pred and FEV1/FVC levels of patients with COPD grade I were higher than those of patients with COPD grades II and III, while the CRP, sCD14, ANXA1, and HPT levels of patients with COPD grade I were lower than those of patients with COPD grades II and III. The abnormal morphology of white blood cells was negatively correlated with FEV1%pred (rs=-0.629, P<0.05) and FEV1/FVC (rs=-0.723, P<0.05), while the abnormal morphology of red blood cells was positively correlated with CRP level (rs=0.594, P<0.05), sCD14 level (rs=0.621, P<0.05), ANXA1 level (rs=0.638, P<0.05), and HPT level (rs=0.680, P<0.05); the AUC of the combined prediction of abnormal morphology of white blood cells and red blood cells was the largest, with an AUC of 0.873(95%CI: 0.806-0.923), a sensitivity of 89.47%, and a specificity of 85.12%. Conclusion Abnormal morphological changes of peripheral blood cells are related to pulmonary function and infection in elderly patients with COPD and pulmonary infection, and have high prognostic value for patients. 2024 Vol. 21 (2): 51-55 [Abstract] ( 94 ) [HTML 1KB] [PDF 2190KB] ( 504 ) 56 LIU Xinglin, ZHANG Hao, WEI Cui'e, LI Ming Botulinum toxin A in the local injection treatment of Raynaud phenomenon in connective tissue disease and efficacy observation Objective To investigate the clinical efficacy and safety of botulinum neurotoxin type A (BTX-A) local injection in the treatment of Raynaud's phenomenon (RP) associated with connective tissue disease. Methods Subjects with no difference in baseline data of both hands were included. Subjects were randomly selected to inject BTX-A in the area of bifurcation of neurovascular bundle around fingers with one hand as the injection side and the other hand as the control side. Visual analogue pain scale (VAS) scores, Raynaud's score (RS) and ulcer score (RS) were recorded at 0, 2, 8 and 12 weeks score, US), average fingertip skin temperature, average capillary filling time and occurrence of adverse reactions were statistically analyzed by SPSS 25.0 software. Results 27 patients completed treatment and follow-up. After 2, 8, and 12 weeks of treatment, there was a statistical difference in the overall mean VAS score between the injection side and the control side. After 8, 12 weeks of treatment, there was a statistically significant difference in the mean Reynolds score between the injection side and the control side. There were no significant differences in Reynolds score at 2 weeks, ulcer score at 2, 8 and 12 weeks, average fingerend skin temperature and average percutaneous oxygen saturation. VAS scores at 2, 8 and 12 weeks of treatment were statistically significant compared with those before treatment, Raynaud scores at 8 and 12 weeks of treatment were statistically significant compared with those before treatment, ulcer scores at 8 and 12 weeks of treatment were statistically significant compared with those before treatment, there was no significant difference in the remaining observation indicators before and after treatment. Conclusion Local injection of botulinum toxin type A can improve the pain of patients with Raynaud, reduce the frequency of Raynaud phenomenon, promote ulcer healing with less adverse reactions, and is a safe, effective, simple and feasible method. 2024 Vol. 21 (2): 56-60 [Abstract] ( 82 ) [HTML 1KB] [PDF 2777KB] ( 562 ) 61 PENG Yuan, ZHU Linfang, ZENG Ying, YUAN Huaihong, ZHAO Wenfang, MAO Mingying Analysis of factors influencing pain in MHD patients Objective Understand the occurrence of pain in maintenance hemodialysis (maintenance hemodialysis, MHD) patients, and explore the current situation of pain and its influencing factors. Methods A total of 345 MHD patients were selected in three tertiary general hospitals in Chengdu from January 2023 to February 2023. Visual analogue scoring (Visual Analogue Scale, VAS), GAD-7 anxiety self-assessment scale, health questionnaire depression self-rating scale (PHQ-9), Frail scale for pain, anxiety, depression, weakness and questionnaire, and analyzed the influencing factors. Results VAS score of MHD patients (3.81±2.25), frailty (2.10±1.76), anxiety (4.71±4.49), and depression (6.63±5.84), and showed significant positive correlation; orderly Logistic regression analysis showed that the influencing factors of pain in MHD patients were frailty, anxiety, depression, and family ability to pay medical expenses. Conclusion High incidence of pain in MHD patients, frailty, anxiety, depression, family ability to pay for medical expenses are the influencing factors of pain in MHD patients. It is recommended that medical staff provide timely assessment of pain in MHD patients, multidisciplinary personalized intervention, and precise care to improve the quality of life of MHD patients. 2024 Vol. 21 (2): 61-65 [Abstract] ( 70 ) [HTML 1KB] [PDF 1941KB] ( 637 ) 66 HUANG Feilong, LI Liang, ZHANG Yi, HU Gui, GUO Yihang, LONG Fei, LIN Changwei, LI Xiaorong Comparative study of functional recovery and quality of life in patients after robotic versus laparoscopic radical rectal cancer surgery Objective To compare the differences in postoperative complications, postoperative anal function, urinary function, and quality of life between robotic and laparoscopic radical rectal cancer surgery. Methods The clinical data of 179 patients who underwent rectal surgery in Xiangya Third Hospital of Central South University from January 2021 to July 2022 were collected, and the patients were divided into the robotic group (37 patients) and laparoscopic group (44 patients) through the inclusion and exclusion criteria and were followed up up up for up to one year, comparing the differences in postoperative complications, anal function, urinary function and quality of life of the two groups of patients. Results There were no intermediate openings and no intraoperative deaths in the two groups. Postoperative pathological data, surgical situation, perioperative recovery, the difference was not statistically significant , the total incidence of complications in the robotic group was lower than that of the laparoscopic group at 1 year after surgery ; anastomotic fistula, intestinal obstruction, and the incidence of reoperation at 1 year after surgery, the difference was not statistically significant ; the symptom scores, functional scores, and the overall health scores of the patients of the two groups were higher than that of the laparoscopic group at 1 month after surgery . Comparison, the difference was not statistically significant; 1 month after surgery, the economic hardship score of the robotic group was higher than that of the laparoscopic group , and the overall nausea and vomiting symptom score of the robotic group was higher than that of the laparoscopic group ; 3 months after surgery, the robotic group had a greater advantage over the laparoscopic group in terms of fatigue, constipation symptom scores, physical function, and overall health status; at 6 months postoperatively and 12 months postoperatively, patients in the robotic group had better overall health than patients in the laparoscopic group; and the cost of hospitalisation was higher in the robotic group than in the laparoscopic group. Conclusion Compared with laparoscopic surgery, robotic surgery for rectal cancer has advantages in reducing the incidence of postoperative complications and improving patients' postoperative quality of life. 2024 Vol. 21 (2): 66-72 [Abstract] ( 108 ) [HTML 1KB] [PDF 2471KB] ( 604 ) 73 SU Dan, ZHANG Li, PAN Dongning Effect of HP eradication therapy on the structure of intestinal flora in children Objective To explore the effect of eradication therapy of Helicobacter pylori (HP) on the structure and interaction of intestinal flora in children. Methods This cross-sectional study was conducted in our hospital from September 2021 to July 2022. Twenty-two children with Helicobacter pylori infection who received bismuth-containing quadruple therapy (BQT) were selected as BQT group, and 23 asymptomatic children who passed 13C urea breath test were recruited as control group. Fecal samples were collected at 0, 2, 6 and 26 weeks. The composition of intestinal bacteria in two groups of children was analyzed and compared by sequencing based on 16S rRNA gene. Results At baseline, there was no significant difference in α diversity (observed species, Chao 1 and Shannon index) and β diversity between BQT group and control group. Compared with the baseline level, the α diversity (species richness, Chao 1 and Shannon index) of patients in BQT group decreased significantly at the second week after eradication, and returned to the baseline level at the 26th week after eradication. PCoA analysis showed that compared with the baseline, there was a significant difference in fecal microbial community structure in BQT group (r=0.998, P=0.001) at the second week, and it was recovered at the sixth week (r=0.051, P=0.12) and the 26th week (r=0.091, P=0.97). Compared with the baseline level, the relative abundance of Firmicutes [（48.23%±15.89%） vs（22.05%±11.88%）, P<0.001] and Bacteroides [（42.99%±15.19%）vs（1.47%±2.49%）, P<0.001] in BQT group decreased significantly at the second week after eradication, while the relative abundance of Proteobacteria increased significantly [（3.13%±3.24%） vs（74.61%±12.28%）, P<0.001]. Compared with the baseline, the abundance of 55 bacterial genera in BQT group changed significantly. Among them, 11 taxa increased significantly and 44 taxa decreased. In the 6th week, 87.3% (48/55) of the significant changes were restored, and in the 26th week, 94.5% (52/55) of the significant changes were restored. In the second week of BQT group, the relative abundance of several beneficial taxa decreased, including Bacteroides, fecal Escherichia, Koala, Roche, Bifidobacterium and Eubacterium myxoides. The relative abundance of some harmful bacteria groups increased, including Escherichia, Klebsiella, Enterococcus and Streptococcus. Conclusion s BQT can cause transient ecological imbalance of intestinal microflora in children with Helicobacter pylori positive. These changes almost restored to the level before eradication after 26 weeks of treatment. 2024 Vol. 21 (2): 73-77 [Abstract] ( 84 ) [HTML 1KB] [PDF 4119KB] ( 528 ) 78 CHEN Wei, YANG Jinhui DLAT: a novel prognostic biomarker and therapeutic target for hepatocellular carcinoma Objective The aim of this study is to investigate the expression and prognostic value of dihydrolipoamide S-acetyltransferase(DLAT) in hepatocellular carcinoma (HCC), and to provide a new biomarker for prognosis evaluation and drug therapy of HCC. Methods The expression, prognostic value and related biological function of DLAT in HCC were analyzed by bioinformatics. Then, the clinicopathological data of 50 HCC patients from Hunan Provincial People's Hospital were collected. The differential expression of DLAT in tumor tissues and adjacent tissues was analyzed by immunohistochemistry, and the prognostic value of DLAT was further analyzed. HCC cell lines HepG2, Huh-7, SMMC-7721 and human normal liver tissue cell line LO2 were cultured, and the expression of DLAT mRNA in each cell line was detected by q-PCR. HepG2 cells were transfected with si-DLAT (experimental group) and si-NC (control group), and CCK8 assay and flow cytometry were used to detect the effect of DLAT on cell proliferation and apoptosis. Results The expression of DLAT in HCC tissues is higher than that in normal tissues, and expression level of DLAT are positively correlated with poor prognosis. The high expression of DLAT in HCC is closely related to the level of immune cell infiltration and cell cycle regulatory genes. After silencing DLAT, the proliferation of HepG2 cells was significantly slower than that of the control group, and the proportion of apoptosis was higher than that of the control group. Conclusion DLAT can be used as a good prognostic marker for patients with HCC, and may become a new target for the treatment of HCC. 2024 Vol. 21 (2): 78-85 [Abstract] ( 108 ) [HTML 1KB] [PDF 10807KB] ( 607 ) 86 LI Na, YANG Mingyuan, CHENG Qinghao Anesthetic effects of propofol and etomidate separately combined with remifentanil target controlled infusion in painless rigid bronchoscopy Objective To investigate the anesthetic effects of propofol and etomidate separately combined with remifentanil target controlled infusion (TCI) in painless rigid bronchoscopy. Methods A total of 60 patients who underwent painless rigid bronchoscopy were divided into propofol (P) group and etomidate (E) group with 30 patients in each group. P group received propofol combined with remifentanil TCI while E group received etomidate combined with remifentanil TCI. The time to fall asleep, wake-up time, vital signs before anesthesia (T0), immediately after the bronchoscope reaching the glottis (T1), immediately after the end of examination (T2) and at 10 min after the end of examination (T3) were compared between the two groups. Stress status was recorded, and the occurrence of adverse reactions was analyzed. Results There was no significant difference in the time to fall asleep or wake-up time between the two groups. Compared with T0, there were increases in mean arterial pressure (MAP), pulse oxygen saturation (SpO2) and heart rate (HR) in both groups at T1 and T2, and decreases at T3. From T1 to T3, MAP, SpO2 and HR in E group were higher than those in P group. The incidence rates of injection pain and hypotension in E group were lower than those in P group. The incidence rates of nausea and vomiting and bradycardia in the two groups were close. Conclusion Applying etomidate combined with remifentanil TCI in painless rigid bronchoscopy can achieve better anesthetic effects. It has obvious advantages in maintaining stable vital signs, and the incidence rates of injection pain and hypotension are lower. 2024 Vol. 21 (2): 86-88 [Abstract] ( 113 ) [HTML 1KB] [PDF 2313KB] ( 576 ) 89 ZHAO Pengyun, XIN Hanxiao, LUO Wei, BAI Xiaojing, SHAO Man Effects and safety of transfusion of washed red blood cells and suspended leukocyte-depleted red blood cells in patients with AIHA Objective To analyze the effects of transfusion of washed red blood cells and suspended leukocyte-depleted red blood cells on patients with autoimmune hemolytic anemia (AIHA). Methods From January 2021 to December 2022, 96 patients with AIHA were admitted to Baoding NO.1 Central Hospital. They were divided into the study group (49 patients washed with washed red blood cells) and the control group (47 patients washed with suspended leukocyte-depleted red blood cells) according to the treatment method. The doses in both groups were 0.1 U/kg, and the infusion rate was 1-3 mL/kg, once every other day. With 3 times of transfusion as a course of treatment, the patients were given 2 courses of treatment. Clinical effects and changes in clinical symptom scores and blood indicators before and after treatment were compared between the two groups. Adverse reactions to transfusion were recorded. Results After treatment, the clinical overall response rate in the study group reached 93.88%, which was significantly higher than 78.72% in the control group . After treatment, the study group had lower scores than the control group for clinical symptoms such as dizziness, shortness of breath, and fatigue. Compared to the control group, hemoglobin (Hb) and red blood cell count (RBC) in the study group were higher. The percentage of reticulocyte (Ret) and total bilirubin (TBIL) in the study group were lower . The incidences of adverse reactions in the study group and the control group were 8.16% and 6.38%, without significant difference between the groups. Conclusion Compared with transfusion of suspended leukocyte-depleted red blood cells, transfusion of washed red blood cells can significantly improve clinical symptoms, hematological and coagulation function indicators in patients with AIHA. 2024 Vol. 21 (2): 89-92 [Abstract] ( 121 ) [HTML 1KB] [PDF 1951KB] ( 694 ) 93 XIAO Lingyi, LIAO Dan, LUO Hongmei, LIU Boyan Curative effect of insulin aspart combined with insulin detemir in the treatment of GDM Objective To explore the curative effect of insulin aspart combined with insulin detemir in patients with gestational diabetes mellitus (GDM). Methods According to random number table method, 98 patients with GDM admitted to Affiliated Hospital of Xiangnan University were divided into control group (49 cases, insulin aspart + protamine biosynthetic human insulin) and observation group (49 cases, insulin aspart combined with insulin detemir) between August 2019 and August 2023. The curative effect, glucose fluctuation indexes [standard deviation of blood glucose fluctuation within 24h (SD), mean of daily differences (MODD), largest amplitude of glycemic excursions (LAGE), mean amplitude of glycemic excursions (MAGE) ], islet related hormones [C-peptide (C-P), leptin (LP), visfatin (VF), adiponectin (APN) ], islet cells function [fasting insulin (FINS), homeostasis model assessment insulin resistance (HOMA-IR), insulin sensitivity index (ISI), homeostasis model assessment insulin resistance β (HOMA-β) ] and incidence of adverse pregnancy outcomes were compared between the two groups. Results After treatment, total response rate of observation group was higher than that of control group. After treatment, SD, MODD, LAGE and MAGE were decreased in both groups, which were lower in observation group than control group. After treatment, C-P, APN, HOMA-β and ISI were increased, while VF, LP, FINS and HOMA-IR were decreased in both groups, and the improvement was better in observation group than control group. The incidence of adverse pregnancy outcomes in observation group than control group was lower than that in control group. Conclusion Curative effect of insulin aspart combined with insulin detemir is significant on patients with GDM, which can effectively reduce blood glucose fluctuation, improve levels of islet related hormones, promote the recovery of islet cells function and reduce the incidence of adverse pregnancy outcomes. 2024 Vol. 21 (2): 93-97 [Abstract] ( 105 ) [HTML 1KB] [PDF 1935KB] ( 619 ) 98 WU Zhao, SUN Rongxiang, ZHANG Yongjun, ZENG Zhao The clinical efficacy of pedicle cortical bone tunnel (CBT) screws combined with pedicle screws in the treatment of elderly degenerative lumbar spinal stenosis Objective To observe the clinical effect of transpedicle cortical bone channel (CBT) screw combined with pedicle nail in the treatment of senile degenerative lumbar spinal stenosis. Methods A total of 40 elderly patients with degenerative lumbar spinal stenosis who failed to receive conservative treatment in Xiangdong Hospital Affiliated to Hunan Normal University from March 2020 to March 2023 were selected and randomly divided into control group (20 cases) and observation group (20 cases) to receive lumbar posterior decompression and bone grafting internal fixation treatment. The control group was treated with single pedicle screw internal fixation. Observation group was treated with CBT screw combined with pedicle screw. Postoperative recovery, operation time, intraoperative blood loss, postoperative drainage volume, VAS score before and after operation, JOA score and so on were observed in both groups. Results The operative time, intraoperative blood loss and postoperative drainage volume in observation group were lower than those in control group. Symptoms were improved in both groups, with VAS score lower after surgery and JOA score higher than before surgery. The VAS scores of lumbago and lower limb pain in the observation group were lower than those in the control group 1 week after surgery, but there was no significant difference in VAS scores between the two groups 6 months after surgery. There was no significant difference in JOA scores between the two groups before surgery, 1 week after surgery and 6 months after surgery. There were no complications such as dural sac rupture, cerebrospinal fluid leakage, nerve injury or wound infection in the two groups. No loosening, falling off or collapse of screws were observed on X-ray films in both groups at 6 months after operation. Conclusion CBT screw combined with pedicle screw technique for lumbar internal fixation in the treatment of senile degenerative lumbar spinal stenosis has the advantages of small exposure area, less blood loss, short operation time, little muscle and soft tissue damage, strong screw holding, etc. The early curative effect is satisfactory, and has certain popularization and application value. 2024 Vol. 21 (2): 98-101 [Abstract] ( 76 ) [HTML 1KB] [PDF 2398KB] ( 533 ) 102 NI Ping, PENG Jing, LI Hua, HUANG Le, HUANG Lihui Influence of husband accompanying delivery on the outcome of parturient and postpartum depression Objective Exploring the impact of husband companionship during childbirth on the delivery outcomes and postpartum depression of parturients. Method 660 primiparous women who gave birth in our hospital from August 1, 2021 to November 1, 2021 were selected as the research subjects. A randomized controlled trial method was used to divide them into an intervention group (husband cooperating with midwife for delivery) and a control group (only midwife for delivery). Observe the differences in delivery outcomes and psychological conditions such as postpartum depression and anxiety between two groups of parturients. Results The intervention group had lower total labor process, postpartum hemorrhage, conversion rate to cesarean section, vaginal delivery rate, and neonatal asphyxia rate compared to the control group; Although the incidence of postpartum depression and anxiety in the intervention group was slightly higher, the difference was not statistically significant (P>0.05). Conclusion The postpartum outcome of the husband's participation in the accompanying delivery is good, and does not increase the incidence of postpartum depression. However, it is necessary to further standardize the process of the husband's accompanying delivery, strengthen training and education, improve the satisfaction and social effect of the husband's participation in the accompanying delivery, and increase the maternal willingness to reproduce. 2024 Vol. 21 (2): 102-106 [Abstract] ( 109 ) [HTML 1KB] [PDF 1937KB] ( 599 ) 107 DENG Tianjiao, JIANG Yafen, ZHANG Qingyun, WU Rong, LI Zhengqi, ZHANG Lin Prediction of survival and prognosis of maintenance hemodialysis patients based on nomogram Objective To develop and validate an easy-to-use assessment tool for predicting short-term survival in maintenance hemodialysis (MHD) patients. Method The clinical data of MHD patients undergoing regular dialysis at the Dialysis Center of the Fourth Hospital of Changsha City from January 2018 to December 2022 were retrospectively analyzed. A total of 543 MHD patients were enrolled and randomly allocated into the training cohort (380 patients) and the validation cohort (163 patients) at a 7: 3 ratio. Least absolute shrinkage and selection operator (LASSO) regression and Cox multifactor regression analysis were employed to identify predictors and construct a nomogram. The nomogram's performance was assessed through the C-index, receiver operating curve (ROC), area under the curve (AUC), and calibration curve. Results Cox multivariate regression analysis revealed that age (HR=1.03, 95% CI 1.01-1.05), duration of dialysis (HR=0.95, 95% CI 0.93-0.96), serum ferritin<21.8 ng/mL (HR=0.68, 95% CI 0.09-4.98), 274.66 ng/mL<serum ferritin ≤500 ng/mL (HR=1.72, 95% CI 0.94-3.12), and serum ferritin>500 ng/mL (HR=2.36, 95% CI 1.50-3.72) were statistically significant. The cross-validation on the nomogram demonstrated a C-index of 0.802 for the training cohort and 0.838 for the validation cohort, indicating a strong discriminatory capability. Discrimination ability in both cohorts was favorable (the 1-year AUC in the training cohort and the validation cohort were 0.909 and 0.948, respectively; The 3-year AUC was 0.899 and 0.928, respectively). Calibration curves for both training and validation cohorts exhibited excellent alignment, underscoring the model's robust predictive performance. Conclusion Utilizing three widely employed clinical indicators—age, duration of dialysis, and serum ferritin. We developed a nomogram capable of predicting the 1-year and 3-year survival rates of MHD patients. This nomogram holds significant clinical potential and can be effectively applied in clinical practice. 2024 Vol. 21 (2): 107-113 [Abstract] ( 87 ) [HTML 1KB] [PDF 3231KB] ( 644 ) 114 WU Jinhua, JIA Linping, LI Jing Effect of Wuhu Decoction on acute asthmatic bronchitis caused by mycoplasma infection in children and its influence on Th1/Th2 cytokines Objective To investigate the curative effect of modified Wuhu Decoction on acute asthmatic bronchitis infected with mycoplasma in children and its effect on Helper T cells (Th) 1/Th2 cytokines. Methods Prospective study. A total of 80 children with acute asthmatic bronchitis infected with phlegm-heat closed-lung mycoplasma treated in our hospital from March 2020 to March 2023 were selected and divided into two groups by random number table method. Control group (40 cases) was treated with azithromycin anti-infection combined with ambroxol and pramicol Ling Shu for 1 week, observation group (40 cases) was treated with supplemented Wuhu Decoction for 1 week on the basis of control group. Compared the therapeutic effect of the two groups, traditional Chinese medicine syndrome score, normal body temperature recovery time, disappearance time of lung crackles, disappearance time of cough and asthma, interferon-γ (IFN-γ), Tumor necrosis faction-α (TNF-α), Th2 cytokines interleukin (IL) -4, IL-6, adverse reactions. Results The effective rate of the observation group was higher than that of the control group, the time of body temperature returning to normal, the time of lung rales disappearing, the time of cough and asthma disappearing was shorter than that of the control group. The serum IFN-γ, TNF-α, IL-4 and IL-6 levels of the two groups after treatment were lower than those before treatment, and the serum difference of TCM syndrome score, IFN-γ, TNF-α, IL-4 and IL-6 levels of the observation group after treatment were lower than those of the control group (P<0.05). Conclusion Compared with Western medicine alone, adding Wuhu decoction can improve Th1/Th2 cytokine disorder in children with acute asthmatic bronchitis infected with phlegm-heat cloister-pulmonary mycoplasma and improve clinical efficacy. 2024 Vol. 21 (2): 114-117 [Abstract] ( 88 ) [HTML 1KB] [PDF 1898KB] ( 636 ) 118 CHEN Yuewei, XU Li, LIAO Hongmei, YI Mi, CHEN Mei, LIU Huayan Analysis of the Burden and Influencing Factors of Caregivers of Children with Duchenne Muscular Dystrophy Objective To investigate the burden of caregivers of children with Duchenne Muscular Dystrophy (DMD) and analyze its influencing factors. Method This was a prospective study, and the convenience sampling method was used . A total of 196 cases of children with DMD and their caregivers who sought medical treatment at a tertiary children's hospital in Hunan Province from January 1, 2019, to December 30, 2022, as the study subjects. General information questionnaires, caregiver burden scales, and social support rating scale were used for data collection. Multiple linear regression analysis was conducted to explore the influencing factors of the burden that caregivers of children with DMD carried. Results The total score of the burden scale for caregivers of children with DMD was (51.561±17.12), with 1 case (0.51%) classified as having no burden, 51cases (26.02%) as mild burden, 62 cases (31.63%) as moderate burden, and 82 cases (41.84%) as severe burden. Pearson correlation analysis showed that caregiver burden was negatively correlated with social support. Multiple linear regression analysis revealed that the self-care ability of the child, family income, parental education level, and social support were significant factors influencing the burden of caregivers of children with DMD, accounting for 95.9% of the total variation. Conclusion The burden of caregivers of DMD children is at a moderate to severe level. The main factors affecting the burden of caregivers of DMD children are the self-care ability of children, annual family income, education level of caregivers and social support level. Medical staff should take a variety of measures from multiple directions and perspectives to improve caregivers' awareness of DMD disease and strengthen social support intervention, so as to reduce the burden of caregivers of DMD children. 2024 Vol. 21 (2): 118-123 [Abstract] ( 80 ) [HTML 1KB] [PDF 1969KB] ( 585 ) 124 JIANG Yafen, WU Rong Analysis of clinical efficacy of Isomaltoside iron in patients with maintenance hemodialysis combined with iron defificiency anaemia Objective To explore the clinical effificacy of Isomaltoside Iron in patients with maintenance hemodialysis (MHD) combined with iron defificiency anaemia (IDA). Methods 61patients with MDH combined with IDA in our hospital's blood purification center from June 2021 to June 2022 were selected and divided into sucrose iron group (29 cases) and isomaltoside iron group (32 cases) according to different treatment drugs. Patients in the sucrose iron group were treated with Sucrose Iron, while those in the isomaltoside iron group were treated with Isomaltoside Iron. After 3 months of continuous treatment, the changes in biochemical indicators (urea nitrogen, creatinine, PTH, hemoglobin, ferritin, transferrin saturation) and adverse reaction were compared between the two groups. Results Before treatment, there was no statistically significant difference between the clinical indicators of the two groups. After 3 months of treatment, there was no statistically significant difference in serum BUN, Scr, UA and PTH between the two groups. both the two groups had an increase in hemoglobin、ferritin and transferrin saturation levels, and the isomaltoside iron Group increased more significantly than sucrose iron group, which was statistically significant. At the same time, There is no statistically significant difference in the incidence of adverse events between the two groups of patients. Conclusion Compared with sucrose iron, isomaltoside iron induced a non-inferior haematological response, comparably low rates of hypersensitivity reactions, and exhibits faster effects, without increasing the incidence of adverse reactions。 2024 Vol. 21 (2): 124-126 [Abstract] ( 89 ) [HTML 1KB] [PDF 2343KB] ( 533 ) 127 SHAO Lijie, ZHU Xiang, ZHU Wenjing, YIN Shi, CHEN Yanyan, WANG Lanlan Analysis of The Factors of Blood-related Indicators, Body Composition, and Nutritional Status That Influence on The Accuracy of Flash Glucose Monitoring Equipment Objective This study aims to explore risk factors impacting the accuracy of flash glucose monitoring (FGM) by analyzing various parameters including blood routine, biochemical indicators, blood glucose, body composition, and nutritional status among patients. Methods Patients were selected with type 2 diabetes who were hospitalized in the department of Gerontology at the First Affiliated Hospital of University of Science and Technology of China (Anhui Provincial Hospital) from April 2021 to April 2023. Patients' general information was collected, such as blood-related indicators, Body composition, malnutrition, and other parameters. FGM glucose readings were compared with fasting and 2-hour postprandial venous blood glucose respectively. Based on the paired blood glucose mean relative error (MARD) with its value 15% as a threshold, those with MARD less than 15% are the accurate group (MARD<15%), and those with more than 15% are the inaccurate group (MARD>15%). Among them, the patients were divided into the fasting FGM accurate group (n=25) and inaccurate group (n=18), and 2-hour postprandial FGM accurate group (n=32) and inaccurate group (n=11). Clarke grid analysis was used to evaluate the accuracy of FGM. Binary logistic regression analysis was performed to identify the independent risk factors that influence the accuracy of FGM. Results Forty-three subjects were included, yielding 86 pairs of blood glucose values. Clarke grid error analysis was performed on the FGM glucose values with venous blood glucose as the reference value. The results showed that 90.7% falls in area A, 9.3% falls in area B, and 100 % falls in area A+B, with an average MARD of 11.7%. Our results showcase that malnutrition is an independent risk factor affecting fasting FGM with an OR value of 7.979(95%CI 1.540-41.335, P=0.013) and it is also an independent risk factor affecting the accuracy of postprandial FGM with an OR value of 7.769(95% CI 1.117-59.370, P=0.048). Conclusion The FGM sensor used in this study met international standards for accuracy. Although the venous blood glucose is very different for a few individual patients during testing process, the overall accuracy for all patients is relatively high. Malnutrition was identified as a independent risk factor impacting FGM accuracy for fasting and 2-hour postprandial glucose levels, while age, body composition, and other blood routine and biochemical indicators showed no significant association with FGM accuracy. 2024 Vol. 21 (2): 127-132 [Abstract] ( 85 ) [HTML 1KB] [PDF 2172KB] ( 592 ) Laboratory Medicine 133 TAN Jie, DENG Zhongping, ZHENG Fang, DING Feng, LIU Rangjiao, TIAN Xiangrong Preliminary evaluation of the performance and clinical application of a domestic quantitative RT-PCR assay for Detection of HIV-1 nucleic acid Objective To preliminarily evaluate the detection performance and clinical applicability of a newly developed Real-Time Quantitative PCR Kit for HIV-1 RNA detection (referred to as Sansure HIV-1). Methods The genotype detection ability, limit of detection, specificity and quantitative detection accuracy of the Sansure HIV-1 were investigated by using WHO HIV-1 serum, National Standards of HIV-1 RNA and clinical samples positive for blood-borne infection pathogens collected by SANWAY Clinical Laboratories INC as samples. The remaining 374 of clinical samples tested by Roche Cobas TaqMan HIV-1 Version 2.0 (referred to as Cobas HIV-1) were collected to evaluate the clinical diagnosis performance of Sansure HIV-1. Statistical methods such as correlation and Bland-Altman analysis were used to compare the performance of the two assays. Results (1) The 10 cases of WHO Subtype Blood inventory detected by Sansure HIV-1 were all positive. (2) The absolute deviation between the logarithmic value of each sample concentration and the theoretical logarithmic value did not exceed ±0.4 log orders of magnitude. (3) The positive rates were 100% when the HIV-1 RNA National Standards were diluted to 50 IU/mL and 25 IU/mL, and the positive rate was 75% when diluted to 12.5 IU/mL. All clinically similar pathogen were tested negative using Sansure HIV-1, and no cross-reaction occurred. (4) The positive rates of Cobas HIV-1 and Sansure HIV-1 in testing 374 clinical samples were both 66.84% (250/374) . (5) Taking the Cobas HIV-1 test results as a reference, the positive consistency of the Sansure HIV-1 reached 99.20% (95%CI: 96.82%-99.86%) , and the negative consistency was 98.38% (95%CI: 93.71%-99.72%) , and the total consistency was 98.93% (95%CI: 94.04%-99.75%) . (6) The linear regression equation of 248 samples tested positive by both assays was y=0.8357x+0.7379, and the r was 0.8901 (P<0.0001) . (7) Further, Bland-Altman analysis showed the SD was 0.5606 (Log10 IU/ mL) , confirmed that the RT-PCR quantitative results detected by the two reagents were highly consistent. Conclusion The evaluated Sansure HIV-1 assay has good quantitative accuracy and good subtype detection coverage for HIV group M, Group O and group N with a detection sensitivity of 25 IU/mL. There is no cross-reaction with common clinical blood-borne pathogens. Sansure HIV-1 showed great consistency in terms of positive detection rate and quantitative results compared with Roche Cobas HIV-1 in testing clinical plasma samples, Considering cost-effectiveness and accessibility, the Sansure HIV-1 assay kit has good clinical applicability and can suitable for the requirements of most clinical applications. 2024 Vol. 21 (2): 133-138 [Abstract] ( 108 ) [HTML 1KB] [PDF 2602KB] ( 503 ) 139 JIAO Fangyan, ZHANG Yu, ZOU Guoying, JIANG Weiping, XU Fei Medical testing laboratories applying critical Sigma value to establish appropriate internal quality control schemes for quantitative tests Objective In order to avoid the possible lack of control or over-control in the design of internal quality control (IQC) scheme, an appropriate IQC scheme based on critical sigma value and meeting the quality control (QC) performance objectives was established for the Medical laboratory. Method By using QC software and office software, the probability of error detection (Ped) and the probability of false rejection (Pfr) of common QC rules were calculated when the system error ranged from 0 to 4.50s. The sigma metric graph and the operational process specifications (OPSpecs) charts were drawn. Results The critical sigma value was obtained when Ped was 90% and Pfr was less than 5%, and the appropriate QC scheme was obtained. The standardized OPSpecs charts were drawn when the analysis quality assurance was 90%. Conclusion The IQC scheme based on the critical sigma value can effectively ensure the Ped of the QC scheme and maintain a low Pfrfor the Medical laboratory, so as to prevent lack of control or over-control. It is not recommended to use only one level of QC material, which is not conducive to the use of QC rules, and can not provide quality assurance for low-medium sigma level tests. 2024 Vol. 21 (2): 139-142 [Abstract] ( 83 ) [HTML 1KB] [PDF 2984KB] ( 633 ) Nursing Science 143 LIANG Gaoke, WANG Weihong Analysis of the self-efficacy status and influencing factors of cardiac rehabilitation exercise for patients with coronary heart disease in rural areas of Hunan Province Objective To understand the current status of self-efficacy in cardiac rehabilitation exercise for patients with coronary heart disease in rural areas of Hunan Province, analyze its influencing factors, and provide reference for the development of relevant intervention measures in the future. Methods A stratified random sampling method was used to select 408 rural coronary heart disease patients in Hunan Province who met the inclusion and exclusion criteria from April 2023 to October 2023 as the study subjects. The study subjects were surveyed using a general information survey form and a self-efficacy scale for coronary heart disease rehabilitation exercise. The data were analyzed using SPSS 26.0 statistical software. Results The total self-efficacy score of cardiac rehabilitation exercise for 408 rural coronary heart disease patients in Hunan Province was (37.98±3.84), with a scoring rate of 47.48%. The results of univariate analysis showed that patients of different ages, genders, monthly incomes, and comorbidities had statistically significant differences in self-efficacy scores of cardiac rehabilitation exercise; The results of logistic regression analysis show that age and educational level are the main influencing factors of self-efficacy in cardiac rehabilitation exercise for patients with coronary heart disease in rural areas of Hunan Province. Conclusion The self-efficacy of cardiac rehabilitation exercises for rural patients with coronary heart disease in Hunan Province is generally at a medium-low level, and attention should be focused on older and less educated patients with coronary heart disease to provide them with targeted health education to enhance their self-efficacy in cardiac rehabilitation exercises. 2024 Vol. 21 (2): 143-148 [Abstract] ( 88 ) [HTML 1KB] [PDF 1916KB] ( 670 ) 149 WANG Lulu, WU Li Application value of interventional stratified nursing pathway regimen in interventional treatment of patients with acute myocardial infarction complicated with arrhythmia Objective To explore the application value of interventional stratified nursing pathway regimen in the interventional treatment of patients with acute myocardial infarction (AMI) complicated with arrhythmia. Methods 107 patients with AMI complicated with arrhythmia admitted to our hospital from January 2022 to February 2023 were selected as research subjects, and were divided into control group and study group by the random number table method. The control group (53 cases) adopted routine nursing regimen, and the study group (54 cases) adopted interventional stratified nursing pathway regimen. Cardiac function indicators, electrocardiogram parameters, quality of life and occurrence of adverse events were compared between the two groups. Results After intervention, the left ventricular ejection fraction and 6 min walking test distance in the two groups were increased, and the indicators in study group were higher than those in control group. The left ventricular end-diastolic diameter was decreased in both groups after intervention, and the indicator was lower in study group than that in control group. After intervention, the heart rate and QT dispersion of both groups were declined, and the indicators in study group were lower. After intervention, the QT interval was lengthened in both groups, and the QT interval in study group was longer than that in control group. The scores of dimensions of myocardial infarction dimensional assessment scale were declined in both groups after intervention, and the scores were lower in study group. The total incidence rate of adverse events in study group was lower compared to control group. Conclusion The application of interventional stratified nursing pathway regimen in the interventional treatment of patients with AMI and arrhythmia can not only effectively promote the recovery of cardiac function and improve the variation of electrocardiogram, but also enhance the quality of life and reduce the occurrence of adverse events. 2024 Vol. 21 (2): 149-153 [Abstract] ( 80 ) [HTML 1KB] [PDF 1886KB] ( 539 ) 154 LIU Ya, NI Zhichao, LIU Kuikui Effects of multidisciplinary team nursing combined with family support nursing on cognition, anxiety and social support level of family members of premature infants in neonatology department Objective To explore the effects of multidisciplinary team nursing combined with family support nursing on cognition, anxiety and social support level of family members of premature infants in neonatology department. Methods The clinical data were collected from 102 premature infants admitted to neonatology department of the hospital between January 2019 and November 2023. According to different nursing methods, they were divided into routine group (52 cases, routine nursing) and experimental group (50 cases, multidisciplinary team nursing combined with family support nursing). The qualification rate of cognition, psychological state ［generalized anxiety disorder-7(GAD-7), patient health questionnaire-9(PHQ-9) ］, social support ［Social Support Rating Scale (SSRS) ］, adverse events after discharge and nursing satisfaction of family members were compared between the two groups. Results At discharge, qualification rate of patients (primary caregivers) cognition in experimental group was higher than that in routine group (90.00% vs 75.00%, P<0.05). After intervention, scores of GAD-7 and PHQ-9 of patients (primary caregivers) in experimental group were lower than those in routine group. After intervention, scores of objective support, subjective support and social support utilization of patients (primary caregivers), and total score in experimental group were higher than those in routine group. The incidence of adverse events of premature infants after discharge in experimental group was lower than that in routine group (6.00% vs 21.15%, P<0.05), and nursing satisfaction of patients (primary caregivers) was higher than that in routine group (92.00% vs 76.92%, P<0.05). Conclusion Application effect of multidisciplinary team nursing combined with family support nursing is good in premature infants in neonatology department, which can improve cognition, psychological state and social support level of family members, reduce readmission risk after discharge and improve nursing satisfaction. 2024 Vol. 21 (2): 154-158 [Abstract] ( 97 ) [HTML 1KB] [PDF 1888KB] ( 559 ) 159 LIU Yating, HE Pingping, LIU Mengqi, TIAN Dandan, ZHANG Yingqun The Effect of illness intrusiveness on quality of life of the patients with chronic heart failure: Multiple mediating effect of physical activity and psychological distress Objective A multiple mediation model based on the stress action process model was developed to investigate the mediating effects of physical activity and psychological distress on illness intrusiveness and quality of life in patients with chronic heart failure (CHF), as well as to provide a theoretical basis for improving CHF patients' prognoses. Methods A convenience sampling method was used to select 229 CHF patients from two tertiary Grade-A hospitals in Hengyang, Hunan Province, from February to July 2023. A General Information Questionnaire, the Chinese Version of the Illness Intrusiveness Ratings Scale, the International Physical Activity Questionnaire (short volume), the Distress Thermometer, and the Minnesota Living with Heart Failure Questionnaire were used to investigate them. Multiple mediation effect tests were performed using the bias-corrected percentile Bootstrap method (Process plug-in). Results In CHF patients, the illness intrusiveness scores were negatively correlated with physical activity scores and positively correlated with psychological distress scores and quality of life scores; physical activity partially mediated the effect between illness intrusiveness and quality of life, the mediating effect was 0.087, accounting for 14.500% of the total effect value; psychological distress partially mediated the effect between illness intrusiveness and quality of life, the mediating effect was 0.217, accounting for 31.167% of the total effect value; physical activity and psychological distress exerted a multiple mediation effect between illness intrusiveness and quality of life, the mediating effect was 0.067, accounting for 11.166% of the total effect value. Conclusion Illness intrusiveness in CHF patients can impact quality of life through the mediation of physical activity and psychological distress. Healthcare professionals should actively pay attention to illness intrusiveness in CHF patients and, at the same time, take physical activity and psychological distress as intervention targets to promote physical and mental recovery, which in turn will enhance patients' quality of life and improve disease prognosis. 2024 Vol. 21 (2): 159-164 [Abstract] ( 86 ) [HTML 1KB] [PDF 1981KB] ( 689 )

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