USP1在卵巢癌中表达的临床意义及其对卵巢癌细胞恶性表型的影响

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Abstract Objective To investigate the expression of ubiquitin-specific peptidase 1(USP1) in ovarian cancer and its correlation with clinicopathologic features of patients, as well as to explore the impact of USP1 on the malignant phenotype of ovarian cancer cells. Methods The expression level of USP1 in ovarian cancer patients and its relationship with clinical stage were analyzed by bioinformatics. Immunohistochemistry was used to detect the expression of USP1 in ovarian cancer patients and normal ovarian tissues, and to analyze the relationship between its expression level and the clinical characteristics of the patients. The SKOV3 cell line overexpressing USP1 was constructed by lentiviral infection, and the cell proliferation and clone formation abilities were detected by CCK-8 and clone formation assay, while the migration and invasion abilities were detected by Transwell assay. Results The expression level of USP1 in ovarian cancer tissues was significantly higher than that in normal ovarian tissues, and was correlated with the pathological type, peritoneal metastasis and the size or invasion degree of tumor in situ, but was not correlated with the expression levels of Ki67, ER, PR and P53. The results of in vitro experiments showed that USP1 promoted the proliferation, colony formation, migration and invasion ability of ovarian cancer cells. Conclusion USP1 expression was enhanced in ovarian cancer tissues and correlated with patients＇ pathological type, peritoneal metastasis, and clinical stage, and USP1 promoted the progression of the malignant phenotype of ovarian cancer cells.

Key words： ubiquitin specific peptidase 1(USP1) ovarian cancer cell proliferation migration

Received: 26 January 2024

PACS:

R737.31

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	Articles by authors
	YU Bo
	PENG Peng
	HUANG Liang
	BAO Liwei
	WANG Zihan
	WANG Weimin
	ZHANG Xia

Cite this article:

YU Bo,PENG Peng,HUANG Liang等. Clinical significance of USP1 expression in ovarian cancer and its effect on malignant phenotype of ovarian cancer cells[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2024, 21(2): 39-44.

URL:

http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2024/V21/I2/39

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