咪达唑仑通过下调circ_0000285表达影响宫颈癌CaSki细胞增殖和凋亡的实验研究

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Abstract Objective To explore the effect and possible mechanism of midazolam on the proliferation and apoptosis of cervical cancer CaSki cells. Methods CaSki cells were intervened with different doses（0,15,30,60 μg/mL）of midazolam,and the cell proliferation activity and apoptosis were measured by CCK-8 method and flow cytometry,respectively. The protein expressions of pro-caspase3 and cleaved-caspase3 were determined by western blotting,and the expression of circ_0000285 was determined by RT-qPCR. After CaSki cells that interfered with circ_0000285 expression or overexpressed circ_0000285 were constructed by transfecting circ_0000285 small interfering RNA or overexpression vector,they were intervened with 0 or 60 μg/mL midazolam,and then the same method sas above were used to measure cell proliferation activity,apoptosis and the expression of related proteins（Ki-67,pro-caspase3,cleaved-caspase3）. Results After CaSki cells were intervened by midazolam,the proliferation activity and the protein expressions of Ki-67 and pro-caspase3 were decreased,but the apoptosis rate and the protein expressions of cleaved-caspase3 were increased,and the expression of circ_0000285 in the cells was decreased. After interfering the expression of circ_0000285,the proliferation activity and the protein expressions of Ki-67 and pro-caspase3 in CaSki cells were decreased,but the apoptosis rate and the protein expressions of cleaved-caspase3 were increased. Overexpression of circ_0000285 reversed the effects of midazolam on the proliferation and apoptosis of CaSki cells. Conclusion Midazolam may inhibit the proliferation of cervical cancer CaSki cells and promote cell apoptosis by down-regulating circ_0000285.

Key words： cervical cancer midazolam circ_0000285 cell proliferation apoptosis

Received: 07 September 2022

PACS:	R737.33
	R363

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	Articles by authors
	ZHANG Jiangchuan
	WU Dan
	JIANG Wei
	ZHANG Chunmei

Cite this article:

ZHANG Jiangchuan,WU Dan,JIANG Wei等. Experimental study on the effect of midazolam on the proliferation and apoptosis of cervical cancer CaSki cells by down-regulating the expression of circ_0000285[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(5): 38-43.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2023/V20/I5/38

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