LncRNA MIR100HG调控miR-142-5p/SOX5轴对口腔鳞癌细胞增殖、侵袭能力的影响

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Abstract Objective To investigate the effect of the long non-coding RNA mir-100-let-7a-2-ir-125b-1 cluster host gene (LncRNA MIR100HG) regulating miR-14-2-5p / SRY related high mobility box protein 5(SOX 5) axis on the proliferation and invasion capacity of oral squamous cell carcinoma (OSCC) cells. Methods Quantitative real-time PCR and immunoblot were performed to detect the expression of LncRNA MIR100HG, miR-142-5p and SOX 5 in CAL 27, SAS, and SCC-25 in vitro cultured human oral epithelial cells and human OSCC-derived cells. When CAL 27 cells were cultured in vitro, They were randomly divided into control group, LncRNA MIR100HG knockdown group (transfection with LncRNA MIR100HG siRNA plasmid), miR-142-5p mimics group (transfected with miR-142-5p mimics), cotransfection negative control group (transfection with empty plasmid and miR-142-5p negative control), LncRNA MIR100HG knockdown + miR-142-5p inhibitor group (transfected with LncRNA MIR100HG The siRNA plasmid and miR-142-5p inhibitor), After the group-transfection, The expression of LncRNA MIR100HG, miR-142-5p and SOX 5 were analyzed by real-time PCR and immunoblotting; Using the method 3- (4, 5-dimethylthiazole-2) -2, 5-diphenyl tetrazolium bromine salt (MTT) method, 5-ethylene-2-2 ′ -deoxyuridine (Edu) staining and colony formation assay to detect cell proliferation in each group; CAL 27 cell invasion in each group was detected by Transwell invasion assay; Immunoblotting was used to detect protein expression associated with epithelial stromal transformation in each group of CAL 27 cells. OSCC tumor model was constructed in the area near the right armpit of the back of nude mice. The tumor volume and weight of nude mice were detected after 3 weeks of feeding. A dual luciferase reporter assay was used to detect the targeted regulation of miR-142-5p and SOX 5 by miR-142-5p. Results Compared with human oral epithelial cells, LncRNA MIR100HG, SOX 5 protein and mRNA were increased in CAL 27, SAS, SCC-25 and miR-142-5p decreased. In LncRNA MIR100HG knockdown, SOX 5 protein and mRNA expression, cell viability, proliferation rate, colony formation ratio, tumor volume and weight, invasion number, N-cadherin protein expression, miR-142-5p expression than control group. Downregulation of miR-142-5p can weaken the effect of LncRNA MIR100HG knockdown on various indicators of CAL27 cells. Targeted downregulation of miR-142-5p by LncRNA MIR100HG and miR-142-5p and SOX 5 in CAL 27 cells. Conclusion Knockdown of LncRNA MIR100HG can inhibit OSCC cells proliferation, epithelial stromal transformation and invasion by regulating the miR-142-5p/SOX5 axis, and can delay tumor growth in vivo.

Key words： LncRNA MIR100HG miR-142-5p/SOX5 oral squamous cell carcinoma proliferation invasion

Received: 08 December 2022

PACS:

R739.8

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	Articles by authors
	WEI chao
	DONG Zihan
	AN ning
	YANG Qian
	MA Yongping

Cite this article:

WEI chao,DONG Zihan,AN ning等. LncRNA MIR100HG Regulation of the miR-142-5p / SOX 5 axis on the proliferation and invasion ability of oral squamous carcinoma cells[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(5): 44-50.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2023/V20/I5/44

[1] Li H, Zhang Y, Xu M, et al.Current trends of targeted therapy for oral squamous cell carcinoma[J]. J Cancer Res Clin Oncol, 2022, 148(9): 2169-2186.
[2] Nocini R, Vianini M, Girolami I, et al.PD-L1 in oral squamous cell carcinoma: A key biomarker from the laboratory to the bedside[J]. Clin Exp Dent Res, 2022, 8(3): 690-698.
[3] Wu Y, Wang Z, Yu S, et al.LncmiRHG-MIR100HG: A new budding star in cancer[J]. Front Oncol, 2022, 12: 997532-997548.
[4] Liu H, Li D, Sun L, et al.Interaction of lncRNA MIR100HG with hnRNPA2B1 facilitates m6A-dependent stabilization of TCF7L2 mRNA and colorectal cancer progression[J]. Mol Cancer, 2022, 21(1): 74-91.
[5] Wilkins OM, Titus AJ, Salas LA, et al.MicroRNA-Related Genetic Variants Associated with Survival of Head and Neck Squamous Cell Carcinoma[J]. Cancer Epidemiol Biomarkers Prev, 2019, 28(1): 127-136.
[6] Li H, Gai L, Wu Z, et al.Maternal embryonic leucine zipper kinase serves as a potential prognostic marker and leads to sorafenib chemoresistance modified by miR-142-5p in hepatocellular carcinoma[J]. Mol Biol Rep, 2022, 49(4): 3015-3024.
[7] He YZ, Yu SL, Li XN, et al.Curcumin increases crizotinib sensitivity through the inactivation of autophagy via epigenetic modulation of the miR-142-5p/Ulk1 axis in non-small cell lung cancer[J]. Cancer Biomark, 2022, 34(2): 297-307.
[8] Dai D, Feng XD, Zhu WQ, et al.LncRNA BLACAT1 regulates the viability, migration and invasion of oral squamous cell carcinoma cells by targeting miR-142-5p[J]. Eur Rev Med Pharmacol Sci, 2019, 23(23): 10313-10323.
[9] Grimm D, Bauer J, Wise P, et al.The role of SOX family members in solid tumours and metastasis[J]. Semin Cancer Biol, 2020, 67(Pt 1): 122-153.
[10] Li Q, Wang W, Yang T, et al.LINC00520 up-regulates SOX5 to promote cell proliferation and invasion by miR-4516 in human hepatocellular carcinoma[J]. Biol Chem, 2022, 403(7): 665-678.
[11] Zhang S, Wang Q, Li W, et al.MIR100HG Regulates CALD1 Gene Expression by Targeting miR-142-5p to Affect the Progression of Bladder Cancer Cells in vitro, as Revealed by Transcriptome Sequencing[J]. Front Mol Biosci, 2022, 8: 793493-793513.
[12] 李焱. miR-142-5p靶向SOX5对胃癌细胞增殖、凋亡、迁移和侵袭的影响[J]. 现代消化及介入诊疗, 2020, 25(11): 1464-1468.
[13] 邓溪川, 刘璐, 王占利, 等. 甘草苷调控PI3K/Akt/m-TOR信号通路对口腔鳞状细胞癌细胞及裸鼠移植瘤小鼠的作用研究[J]. 实用口腔医学杂志, 2021, 37(1): 10-14.
[14] Brennan PA, Dylgjeri F, Coletta RD, et al.Surgical tumour margins and their significance in oral squamous cell carcinoma[J]. J Oral Pathol Med, 2022, 51(4): 311-314.
[15] Esmeray Sönmez E, Hatipoğlu T, Kurşun D, et al.Whole Transcriptome Sequencing Reveals Cancer-Related, Prognostically Significant Transcripts and Tumor-Infiltrating Immunocytes in Mantle Cell Lymphoma[J]. Cells, 2022, 11(21): 3394-3420.
[16] Li P, Ge D, Li P, et al.CXXC finger protein 4 inhibits the CDK18-ERK1/2 axis to suppress the immune escape of gastric cancer cells with involvement of ELK1/MIR100HG pathway[J]. J Cell Mol Med, 2020, 24(17): 10151-10165.
[17] Zhu C, Jiang X, Xiao H, et al.Tumor-derived extracellular vesicles inhibit HGF/c-Met and EGF/EGFR pathways to accelerate the radiosensitivity of nasopharyngeal carcinoma cells via microRNA-142-5p delivery[J]. Cell Death Discov, 2022, 8(1): 17-27.
[18] Liu J, Jiang X, Zou A, et al.circIGHG-Induced Epithelial-to-Mesenchymal Transition Promotes Oral Squamous Cell Carcinoma Progression via miR-142-5p/IGF2BP3 Signaling[J]. Cancer Res, 2021, 81(2): 344-355.
[19] Chen R, Zhang C, Cheng Y, et al.LncRNA UCC promotes epithelial-mesenchymal transition via the miR-143-3p/SOX5 axis in non-small-cell lung cancer[J]. Lab Invest, 2021, 101(9): 1153-1165.
[20] Le PN, Keysar SB, Miller B, et al.Wnt signaling dynamics in head and neck squamous cell cancer tumor-stroma interactions[J]. Mol Carcinog, 2019, 58(3): 398-410.