MicroRNA表达在宫颈癌及癌前病变中的差异研究

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Abstract Objective To investigate the expression of miRNAs in the blood of patients with squamous cell carcinoma (SCC) and high-grade squamous intraepithelial lesion (HSIL) of the cervix and to determine the impact of these miRNAs in the clinical course of cervical cancer development. Methods Between January 2020 and January 2022, a total of 35 patients with HSIL and 35 patients with cervical SCC and 30 healthy controls were enrolled in the study. Blood specimens from patients were collected and the relative miRNA expression levels of miR-409, miR-32, miR-454, miR-17, miR-520 and miR-373 in serum were analyzed by qRT-PCR. Receiver operating characteristic curve (ROC) was used to analyze the diagnostic potential of differentially expressed miRNAs. Results Compared with healthy controls, miR-409 was found to be significantly down-regulated, and miR-32, miR-454, and miR-17 were significantly up-regulated in HSIL and cervical SCC tissues. In addition, miR-409 was significantly downregulated, and miR-32, miR-454, and miR-17 were significantly upregulated in the cervical SCC group compared with the HSIL group. roC analysis showed that miR-409(AUC=0.972), miR-32(AUC=0.942), and miR-454(AUC=0.905), and miR-17(AUC=0.969) had high specificity and sensitivity in distinguishing the control and SCC groups. miR-409(AUC=0.921) and miR-17(AUC=0.851) had high specificity and sensitivity in distinguishing the HSIL and SCC groups. In addition, miR-32 and miR-454 were less sensitive in distinguishing HSIL group from SCC group. Conclusion In this study, four miRNAs (miR-409, miR-32, miR-454, miR-17) differentially expressed in the sera of normal controls, HSIL and SCC patients are identified, and these miRNAs show good sensitivity and specificity in distinguishing normal from SCC. Among them, miR-409 and miR-17 could also be used as potential markers to distinguish HSIL from SCC.

Key words： microRNA cervical cancer high-grade squamous intraepithelial lesion

Received: 01 November 2022

PACS:

R737.33

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	ZHAO Wenjiao
	LIU Fang
	YAN Qiongqiong

Cite this article:

ZHAO Wenjiao,LIU Fang,YAN Qiongqiong. Differential expression of MicroRNA in cervical cancer and precancerous lesions[J]. HuNan ShiFan DaXue XueBao(YiXueBan), 2023, 20(5): 84-89.

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http://yxb.hunnu.edu.cn/EN/ OR http://yxb.hunnu.edu.cn/EN/Y2023/V20/I5/84

[1] 周斌, 谢福源, 苏学英, 等. 人乳头状瘤病毒与人类疱疹病毒重叠感染在宫颈上皮内瘤变及宫颈癌中的意义[J]. 实用医学杂志, 2020, 36(12): 1654-1658.
[2] 张韶凯, 罗喜平, 李志芳, 等. 人乳头瘤病毒分型检测在宫颈癌前病变和宫颈癌筛查中的应用价值[J]. 中华肿瘤杂志, 2020, 42(3): 252-256.
[3] 龙玉惠, 杨娇娥. 人乳头瘤病毒DNA分型检测宫颈液基薄层细胞学检查及阴道镜活检组织病理学检查在宫颈癌筛查中的应用价值[J]. 中国妇幼保健, 2021, 36(10): 2419-2421.
[4] Nahand JS, Vandchali NR, Darabi H, et al.Exosomal microRNAs: novel players in cervical cancer[J]. Epigenomics, 2020, 12(18): 1651-1660.
[5] 王玮玉, 沈影, 郭滢, 等. miRNAs表达异常在宫颈癌转移机制中的研究进展[J]. 中国细胞生物学学报, 2021, 43(7): 1495-1503.
[6] Andrés-León E, Rojas AM. miARma-Seq, a comprehensive pipeline for the simultaneous study and integration of miRNA and mRNA expression data[J]. Methods, 2019, 152: 31-40.
[7] Shukla V, Varghese VK, Kabekkodu SP, et al.Enumeration of deregulated miRNAs in liquid and tissue biopsies of cervical cancer[J]. Gynecol Oncol, 2019, 155(1): 135-143.
[8] Shen S, Zhang S, Liu P, et al.Potential role of microRNAs in the treatment and diagnosis of cervical cancer[J]. Cancer Genet, 2020, 248: 25-30.
[9] 陈翠云, 王梅云, 朱庆尧, 等. 循环miR-143水平在宫颈癌患者同步放化疗后肿瘤残留的早期预测[J]. 中华放射肿瘤学杂志, 2021, 30(9): 910-916.
[10] Yoshiko Y, Minamizaki T.Emerging roles of microRNAs as extracellular vesicle cargo secreted from osteoblasts[J]. J Oral Biosci, 2020, 62(3): 228-234.
[11] Babion I, Snoek BC, Novianti PW, et al.Triage of high-risk HPV-positive women in population-based screening by miRNA expression analysis in cervical scrapes; a feasibility study[J]. Clin Epigenetics, 2018, 10(1): 76.
[12] Shen S, Zhang S, Liu P, et al.Potential role of microRNAs in the treatment and diagnosis of cervical cancer[J]. Cancer Genet, 2020, 248: 25-30.
[13] Stoen MJ, Andersen S, Rakaee M, et al.High expression of miR-17-5p in tumor epithelium is a predictor for poor prognosis for prostate cancer patients[J]. Sci Rep, 2021, 11(1): 13864.
[14] Wang Q, He Y, Kan W, et al.microRNA-32-5p targets KLF2 to promote gastric cancer by activating PI3K/AKT signaling pathway[J]. Am J Transl Res, 2019, 11(8): 4895-4908.
[15] Wu W, Chen Y, Ye S, et al.Transcription factor forkhead box K1 regulates miR-32 expression and enhances cell proliferation in colorectal cancer[J]. Oncol Lett, 2021, 21(5): 407.
[16] Wang F, Yan J.MicroRNA-454 is involved in regulating trophoblast cell proliferation, apoptosis, and invasion in preeclampsia by modulating the expression of ephrin receptor B4[J]. Biomed Pharmacother, 2018, 107: 746-753.
[17] Zuo J, Yu H, Xie P, et al.miR-454-3p exerts tumor-suppressive functions by down-regulation of NFATc2 in glioblastoma[J]. Gene, 2019, 710: 233-239.
[18] Li Y, Jiao Y, Fu Z, et al.High miR-454-3p expression predicts poor prognosis in hepatocellular carcinoma[J]. Cancer Manag Res, 2019, 11: 2795-2802.
[19] Feng J, Li K, Liu G, et al.Precision hyperthermia-induced miRNA-409-3p upregulation inhibits migration, invasion, and EMT of gastric cancer cells by targeting KLF17[J]. Biochem Biophys Res Commun, 2021, 549: 113-119.